amoebophilus asiaticus  (ATCC)


Bioz Verified Symbol ATCC is a verified supplier
Bioz Manufacturer Symbol ATCC manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Name:
    Acanthamoeba sp
    Description:

    Catalog Number:
    PRA-228
    Price:
    None
    Buy from Supplier


    Structured Review

    ATCC amoebophilus asiaticus
    Abundance of proteins with eukaryotic domains in proteomes of “ Ca . <t>Amoebophilus</t> <t>asiaticus”</t> and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains

    https://www.bioz.com/result/amoebophilus asiaticus/product/ATCC
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amoebophilus asiaticus - by Bioz Stars, 2021-01
    85/100 stars

    Related Products / Commonly Used Together

    trizol
    5a2

    Images

    1) Product Images from "The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria "

    Article Title: The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria

    Journal: Journal of Bacteriology

    doi: 10.1128/JB.01379-09

    Abundance of proteins with eukaryotic domains in proteomes of “ Ca . Amoebophilus asiaticus” and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains
    Figure Legend Snippet: Abundance of proteins with eukaryotic domains in proteomes of “ Ca . Amoebophilus asiaticus” and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains

    Techniques Used:

    Ultrastructure of “ Ca . Amoebophilus asiaticus” 5a2 within its Acanthamoeba host cell. (A) Transmission electron micrograph showing the distribution of “ Ca . Amoebophilus asiaticus” in its Acanthamoeba host cell. (B and C)
    Figure Legend Snippet: Ultrastructure of “ Ca . Amoebophilus asiaticus” 5a2 within its Acanthamoeba host cell. (A) Transmission electron micrograph showing the distribution of “ Ca . Amoebophilus asiaticus” in its Acanthamoeba host cell. (B and C)

    Techniques Used: Transmission Assay

    General features of the “ Ca . Amoebophilus asiaticus” genome.
    Figure Legend Snippet: General features of the “ Ca . Amoebophilus asiaticus” genome.

    Techniques Used:

    Related Articles

    DNA Extraction:

    Article Title: The Endosymbiont Amoebophilus asiaticus Encodes an S-Adenosylmethionine Carrier That Compensates for Its Missing Methylation Cycle
    Article Snippet: .. Acanthamoeba sp. 5a2 (ATCC PRA-228) amoebae harboring A. asiaticus cells were used for DNA isolation with the DNeasy Blood and Tissue kit (Qiagen) according to the manufacturer's recommendations. .. The Aasi_1859 gene, coding for the putative SAM transport protein, was amplified by using High Fidelity PCR enzyme mix (MBI-Fermentas) according to the instructions of the manufacturer.

    Centrifugation:

    Article Title: The Endosymbiont Amoebophilus asiaticus Encodes an S-Adenosylmethionine Carrier That Compensates for Its Missing Methylation Cycle
    Article Snippet: .. Acanthamoeba sp. strain 5a2 (ATCC PRA-228) amoebae harboring A. asiaticus 5a2 cells were harvested by centrifugation (7,000 × g for 3 min at 27°C). .. The resulting cell pellet was resuspended in 750 μl TRIzol (Invitrogen Life Technologies), transferred into a Lysing Matrix A tube (MP Biomedicals), and homogenized by using a BIO101/Savant FastPrep FP120 instrument (speed, 4.5 m/s; 30 s).

    In Vitro:

    Article Title: Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
    Article Snippet: .. Evaluation of the in Vitro Effect of Acanthamoeba in Cell Cultures Cell culture: Madin–Darby canine kidney (MDCK) epithelial cells (NBL2 ATCC CCL-34TM) and Vero cells (ATCC CCL-81) were grown in 75 cm2 cell culture flasks (Corning, Corning Incorporated, NY, USA) with RPMI 1640 medium (Gibco GranIsland, NY, USA) supplemented with penicillin (100 U/mL), streptomycin (100 pg/mL) and 10% fetal calf serum (Gibco, Gran Island, NY, USA). ..

    Isolation:

    Article Title: A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus
    Article Snippet: .. Cultivation and isolation of amoebae Amoebae harboring A. asiaticus 5a2 (ATCC no. PRA-228) and amoebae harboring A. asiaticus EIDS3 (ATCC no. PRA-221) were maintained as adherent culture in 25 cm2 tissue culture flasks containing 10 ml peptone-yeast-glucose medium (PYG: 20 g/l proteose peptone, 2 g/l yeast extract, 90 mM glucose, 4 mM MgSO4 *7H2 O, 3.4 mM C6 H5 Na3 O7 *2H2 O, 2.5 mM KH2 PO4 , 1.3 mM Na2 HPO4 *2H2 O, 51 μM Fe(NH4 )2 (SO4 )2 *6H2 O). .. Cultures were incubated at 27°C and passaged at confluency by 1:10 dilution of the culture every five to ten days.

    Article Title: The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria
    Article Snippet: .. Amoebophilus asiaticus” 5a2 (ATCC number PRA-228) was isolated from lake sediment in Austria ( ) and cultivated using peptone-yeast-glucose-medium (PYG) [containing 20 g/liter proteose peptone, 18 g/liter glucose, 2 g/liter yeast extract, 3.4 mM sodium citrate-dihydrate, 4 mM MgSO4 ·7H2 O, 2 mM Na2 HPO4 ·2H2 O, 1.7 mM KH2 PO4 , 0.05 mM Fe(NH4 )2 (SO4 )2 ·6H2 O] as described previously ( ). .. Amoebae (from 3.6-liters culture) were collected by centrifugation (5,000 × g , 10 min), resuspended in buffer A (35 mM Tris-HCl, 250 mM Sucrose, 25 mM KCl, 10 mM MgCl2 ) containing 250 mM EDTA, and subsequently homogenized using a Dounce tissue grinder (Wheaton).

    Cell Culture:

    Article Title: Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
    Article Snippet: .. Evaluation of the in Vitro Effect of Acanthamoeba in Cell Cultures Cell culture: Madin–Darby canine kidney (MDCK) epithelial cells (NBL2 ATCC CCL-34TM) and Vero cells (ATCC CCL-81) were grown in 75 cm2 cell culture flasks (Corning, Corning Incorporated, NY, USA) with RPMI 1640 medium (Gibco GranIsland, NY, USA) supplemented with penicillin (100 U/mL), streptomycin (100 pg/mL) and 10% fetal calf serum (Gibco, Gran Island, NY, USA). ..

    other:

    Article Title: Insight into the Lifestyle of Amoeba Willaertia magna during Bioreactor Growth Using Transcriptomics and Proteomics
    Article Snippet: If Vermamoeba and Acanthamoeba appear as highly susceptible to a wide range of Legionella spp., previous studies reported the elimination of pathogenic L. pneumophila strain Paris 1 (ATCC 33152) by W. magna C2c maky [ , , , ].

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90
    ATCC amoebophilus asiaticus
    Abundance of proteins with eukaryotic domains in proteomes of “ Ca . <t>Amoebophilus</t> <t>asiaticus”</t> and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains
    Amoebophilus Asiaticus, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amoebophilus asiaticus/product/ATCC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    amoebophilus asiaticus - by Bioz Stars, 2021-01
    90/100 stars
      Buy from Supplier

    Image Search Results


    Abundance of proteins with eukaryotic domains in proteomes of “ Ca . Amoebophilus asiaticus” and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains

    Journal: Journal of Bacteriology

    Article Title: The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria

    doi: 10.1128/JB.01379-09

    Figure Lengend Snippet: Abundance of proteins with eukaryotic domains in proteomes of “ Ca . Amoebophilus asiaticus” and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains

    Article Snippet: Amoebophilus asiaticus” proteins that had their 10 best BLAST hits to proteins from organisms outside the Bacteroidetes but within the same phylogenetic lineage (at the phylum or class level).

    Techniques:

    Ultrastructure of “ Ca . Amoebophilus asiaticus” 5a2 within its Acanthamoeba host cell. (A) Transmission electron micrograph showing the distribution of “ Ca . Amoebophilus asiaticus” in its Acanthamoeba host cell. (B and C)

    Journal: Journal of Bacteriology

    Article Title: The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria

    doi: 10.1128/JB.01379-09

    Figure Lengend Snippet: Ultrastructure of “ Ca . Amoebophilus asiaticus” 5a2 within its Acanthamoeba host cell. (A) Transmission electron micrograph showing the distribution of “ Ca . Amoebophilus asiaticus” in its Acanthamoeba host cell. (B and C)

    Article Snippet: Amoebophilus asiaticus” proteins that had their 10 best BLAST hits to proteins from organisms outside the Bacteroidetes but within the same phylogenetic lineage (at the phylum or class level).

    Techniques: Transmission Assay

    General features of the “ Ca . Amoebophilus asiaticus” genome.

    Journal: Journal of Bacteriology

    Article Title: The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria

    doi: 10.1128/JB.01379-09

    Figure Lengend Snippet: General features of the “ Ca . Amoebophilus asiaticus” genome.

    Article Snippet: Amoebophilus asiaticus” proteins that had their 10 best BLAST hits to proteins from organisms outside the Bacteroidetes but within the same phylogenetic lineage (at the phylum or class level).

    Techniques: