amoebophilus asiaticus (ATCC)

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Acanthamoeba sp

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PRA-228

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Structured Review

ATCC amoebophilus asiaticus
Abundance of proteins with eukaryotic domains in proteomes of “ Ca . <t>Amoebophilus</t> <t>asiaticus”</t> and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains

Abundance of proteins with eukaryotic domains in proteomes of “ Ca . <t>Amoebophilus</t> <t>asiaticus”</t> and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains

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amoebophilus asiaticus - by Bioz Stars, 2021-01

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trizol
5a2

Images

1) Product Images from "The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria "

Article Title: The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria

Journal: Journal of Bacteriology

doi: 10.1128/JB.01379-09

Abundance of proteins with eukaryotic domains in proteomes of “ Ca . Amoebophilus asiaticus” and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains

Figure Legend Snippet: Abundance of proteins with eukaryotic domains in proteomes of “ Ca . Amoebophilus asiaticus” and other bacteria. Proteins with eukaryotic domains are most abundant in intracellular bacteria. The percentage of proteins with eukaryotic domains

Techniques Used:

Ultrastructure of “ Ca . Amoebophilus asiaticus” 5a2 within its Acanthamoeba host cell. (A) Transmission electron micrograph showing the distribution of “ Ca . Amoebophilus asiaticus” in its Acanthamoeba host cell. (B and C)

Figure Legend Snippet: Ultrastructure of “ Ca . Amoebophilus asiaticus” 5a2 within its Acanthamoeba host cell. (A) Transmission electron micrograph showing the distribution of “ Ca . Amoebophilus asiaticus” in its Acanthamoeba host cell. (B and C)

Techniques Used: Transmission Assay

Figure Legend Snippet: General features of the “ Ca . Amoebophilus asiaticus” genome.

Techniques Used:

DNA Extraction:

Article Title: The Endosymbiont Amoebophilus asiaticus Encodes an S-Adenosylmethionine Carrier That Compensates for Its Missing Methylation Cycle
Article Snippet: .. Acanthamoeba sp. 5a2 (ATCC PRA-228) amoebae harboring A. asiaticus cells were used for DNA isolation with the DNeasy Blood and Tissue kit (Qiagen) according to the manufacturer's recommendations. .. The Aasi_1859 gene, coding for the putative SAM transport protein, was amplified by using High Fidelity PCR enzyme mix (MBI-Fermentas) according to the instructions of the manufacturer.

Centrifugation:

Article Title: The Endosymbiont Amoebophilus asiaticus Encodes an S-Adenosylmethionine Carrier That Compensates for Its Missing Methylation Cycle
Article Snippet: .. Acanthamoeba sp. strain 5a2 (ATCC PRA-228) amoebae harboring A. asiaticus 5a2 cells were harvested by centrifugation (7,000 × g for 3 min at 27°C). .. The resulting cell pellet was resuspended in 750 μl TRIzol (Invitrogen Life Technologies), transferred into a Lysing Matrix A tube (MP Biomedicals), and homogenized by using a BIO101/Savant FastPrep FP120 instrument (speed, 4.5 m/s; 30 s).

In Vitro:

Article Title: Isolation of Acanthamoeba T5 from Water: Characterization of Its Pathogenic Potential, Including the Production of Extracellular Vesicles
Article Snippet: .. Evaluation of the in Vitro Effect of Acanthamoeba in Cell Cultures Cell culture: Madin–Darby canine kidney (MDCK) epithelial cells (NBL2 ATCC CCL-34TM) and Vero cells (ATCC CCL-81) were grown in 75 cm2 cell culture flasks (Corning, Corning Incorporated, NY, USA) with RPMI 1640 medium (Gibco GranIsland, NY, USA) supplemented with penicillin (100 U/mL), streptomycin (100 pg/mL) and 10% fetal calf serum (Gibco, Gran Island, NY, USA). ..

Isolation:

Article Title: A bacterial genome in transition - an exceptional enrichment of IS elements but lack of evidence for recent transposition in the symbiont Amoebophilus asiaticus
Article Snippet: .. Cultivation and isolation of amoebae Amoebae harboring A. asiaticus 5a2 (ATCC no. PRA-228) and amoebae harboring A. asiaticus EIDS3 (ATCC no. PRA-221) were maintained as adherent culture in 25 cm2 tissue culture flasks containing 10 ml peptone-yeast-glucose medium (PYG: 20 g/l proteose peptone, 2 g/l yeast extract, 90 mM glucose, 4 mM MgSO4 *7H2 O, 3.4 mM C6 H5 Na3 O7 *2H2 O, 2.5 mM KH2 PO4 , 1.3 mM Na2 HPO4 *2H2 O, 51 μM Fe(NH4 )2 (SO4 )2 *6H2 O). .. Cultures were incubated at 27°C and passaged at confluency by 1:10 dilution of the culture every five to ten days.

Article Title: The Genome of the Amoeba Symbiont “Candidatus Amoebophilus asiaticus” Reveals Common Mechanisms for Host Cell Interaction among Amoeba-Associated Bacteria
Article Snippet: .. Amoebophilus asiaticus” 5a2 (ATCC number PRA-228) was isolated from lake sediment in Austria ( ) and cultivated using peptone-yeast-glucose-medium (PYG) [containing 20 g/liter proteose peptone, 18 g/liter glucose, 2 g/liter yeast extract, 3.4 mM sodium citrate-dihydrate, 4 mM MgSO4 ·7H2 O, 2 mM Na2 HPO4 ·2H2 O, 1.7 mM KH2 PO4 , 0.05 mM Fe(NH4 )2 (SO4 )2 ·6H2 O] as described previously ( ). .. Amoebae (from 3.6-liters culture) were collected by centrifugation (5,000 × g , 10 min), resuspended in buffer A (35 mM Tris-HCl, 250 mM Sucrose, 25 mM KCl, 10 mM MgCl2 ) containing 250 mM EDTA, and subsequently homogenized using a Dounce tissue grinder (Wheaton).

Cell Culture:

other:

Article Title: Insight into the Lifestyle of Amoeba Willaertia magna during Bioreactor Growth Using Transcriptomics and Proteomics
Article Snippet: If Vermamoeba and Acanthamoeba appear as highly susceptible to a wide range of Legionella spp., previous studies reported the elimination of pathogenic L. pneumophila strain Paris 1 (ATCC 33152) by W. magna C2c maky [ , , , ].