aminoallyl dutp nucleotides  (Jena Bioscience)


Bioz Verified Symbol Jena Bioscience is a verified supplier
Bioz Manufacturer Symbol Jena Bioscience manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 85
    Name:
    Aminoallyl dUTP Solution
    Description:

    Catalog Number:
    NU-803L
    Price:
    291.31
    Category:
    Nucleotides Nucleosides
    Size:
    5 x 10 µl
    Buy from Supplier


    Structured Review

    Jena Bioscience aminoallyl dutp nucleotides

    https://www.bioz.com/result/aminoallyl dutp nucleotides/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aminoallyl dutp nucleotides - by Bioz Stars, 2021-06
    85/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Using Amino-Labeled Nucleotide Probes for Simultaneous Single Molecule RNA-DNA FISH
    Article Snippet: Nick translation labeled probes were prepared using nick translation kit (Roche, Cat# 10976776001). .. Nucleotide analogs used in probe labeling were Cy3-dUTP (Amersham, Cat# PA53022) and aminoallyl-dUTP (Jena Bioscience, Cat# NU-803S). .. Mouse Xist RNA was detected with Sx9 probe, a P1 DNA construct containing a 40 kb genomic fragment covering the mouse X inactivation centre (Xic ) region.

    Article Title: Rad50-CARD9 interactions link cytosolic DNA sensing to IL-1β production
    Article Snippet: For presentation, the images were processed with Image J software ) using linear contrast enhancement on entire images. .. The direct labeling of double-stranded poly(dG:dC) DNA by incorporating ATTO 647N fluorescently labeled aminoallyl-dUTP nucleotides (Jena Bioscience) was performed by nick translation, as previously described . .. Cytokine concentrations were determined by ELISA (BD Biosciences, eBioscience, or PBL Biomedical Laboratories) or Cytometric Bead Array (CBA, BD Biosciences) according to the manufacturer’s instructions.

    Nick Translation:

    Article Title: Rad50-CARD9 interactions link cytosolic DNA sensing to IL-1β production
    Article Snippet: For presentation, the images were processed with Image J software ) using linear contrast enhancement on entire images. .. The direct labeling of double-stranded poly(dG:dC) DNA by incorporating ATTO 647N fluorescently labeled aminoallyl-dUTP nucleotides (Jena Bioscience) was performed by nick translation, as previously described . .. Cytokine concentrations were determined by ELISA (BD Biosciences, eBioscience, or PBL Biomedical Laboratories) or Cytometric Bead Array (CBA, BD Biosciences) according to the manufacturer’s instructions.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Jena Bioscience cy5 modified
    Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with <t>Cy5-fluorophores</t> (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).
    Cy5 Modified, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cy5 modified/product/Jena Bioscience
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cy5 modified - by Bioz Stars, 2021-06
    99/100 stars
      Buy from Supplier

    85
    Jena Bioscience aminoallyl dutp nucleotides
    Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with <t>Cy5-fluorophores</t> (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).
    Aminoallyl Dutp Nucleotides, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aminoallyl dutp nucleotides/product/Jena Bioscience
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aminoallyl dutp nucleotides - by Bioz Stars, 2021-06
    85/100 stars
      Buy from Supplier

    93
    Jena Bioscience fluorescent nucleotides atto 550 dutp aminoallyl dutp
    Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with <t>Cy5-fluorophores</t> (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).
    Fluorescent Nucleotides Atto 550 Dutp Aminoallyl Dutp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fluorescent nucleotides atto 550 dutp aminoallyl dutp/product/Jena Bioscience
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fluorescent nucleotides atto 550 dutp aminoallyl dutp - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with Cy5-fluorophores (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).

    Journal: eLife

    Article Title: DNA sequence encodes the position of DNA supercoils

    doi: 10.7554/eLife.36557

    Figure Lengend Snippet: Direct visualization of individual plectonemes on supercoiled DNA. ( a ) Schematic of the ISD assay. (top) A flow-stretched DNA is doubly-tethered on a PEG-coated surface via streptavidin-biotin linkage. One-end of the DNA is labeled with Cy5-fluorophores (red stars) for identifying the direction of each DNA molecule. (bottom) Binding of SxO fluorophores induces supercoiling to the torsionally constrained DNA molecule. ( b ) Representative fluorescence images of a supercoiled DNA molecule. Left: Snap-shot image of a supercoiled DNA with 100 ms exposure. Yellow arrows highlight higher DNA density, that is individual plectonemes. Right: Time-averaged DNA image by stacking 1000 images (of 100 ms exposure each). Arrows indicate peaks in the inhomogeneous average density of plectonemes. ( c ) AT-contents of two DNA samples: template1 and template2 binned to 300 bp. ( d ) Plectoneme densities obtained from individual DNA molecules. (top) Plectoneme density on template1 (grey thin lines, n = 70) and their ensemble average (red line). Arrow indicates a strong plectoneme pinning site. (bottom) Plectoneme densities obtained from individual DNA molecules of template2 (grey thin lines, n = 120) and their ensemble average (black line).

    Article Snippet: The ‘Cy5-biotin handle’ and ‘biotin handle’ were prepared by PCR methods in the presence of Cy5-modified and/or biotinylated dUTP (aminoallyl-dUTP-Cy5 and biotin-16-dUTP, Jena Bioscience).

    Techniques: Flow Cytometry, Labeling, Binding Assay, Fluorescence, Mass Spectrometry