amine peg3 biotin  (Thermo Fisher)


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    Name:
    EZ Link Amine PEG3 Biotin
    Description:
    Thermo Scientific EZ Link Amine PEG3 Biotin is a water soluble biotin compounds containing a polyethylene glycol PEG spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods Features of EZ Link Amine PEG3 Biotin • Biotinylation label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins • Amine activated primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers • Pegylated polyethylene glycol PEG groups in spacer arm enhances water solubility of biotinylated molecules • Medium length spacer arm total length added to target is 22 9 angstroms Amine PEG2 Biotin and Amine PEG3 Biotin are the shorter two of three amine modified biotin compounds that contain polyethylene glycol PEG spacer arms The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini aspartate residues or glutamate residues using EDC Part No 22980 a water soluble carbodiimide crosslinker EDC activates carboxyl groups to bind to the NH2 group of the amino biotin forming an amide bond We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications Amino biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways The most common method is to crosslink the terminal primary amine to carboxyl groups using Carboxyl groups COOH occur in aspartate or glutamate residues and the carboxy terminus of polypeptides When activated with EDC Part No 22980 carboxylates react with amino NH2 groups to form amide bonds Carboxylate molecules and surface materials can be pre activated using EDC with Sulfo NHS Part No 24510 for subsequent reaction to primary amines see NHS ester Chemistry Related Products EZ Link Amine PEG2 Biotin
    Catalog Number:
    21347
    Price:
    None
    Applications:
    Protein Biology|Protein Labeling|Protein Labeling & Crosslinking
    Category:
    Labeling Detection Products
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    Structured Review

    Thermo Fisher amine peg3 biotin
    Thermo Scientific EZ Link Amine PEG3 Biotin is a water soluble biotin compounds containing a polyethylene glycol PEG spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods Features of EZ Link Amine PEG3 Biotin • Biotinylation label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins • Amine activated primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers • Pegylated polyethylene glycol PEG groups in spacer arm enhances water solubility of biotinylated molecules • Medium length spacer arm total length added to target is 22 9 angstroms Amine PEG2 Biotin and Amine PEG3 Biotin are the shorter two of three amine modified biotin compounds that contain polyethylene glycol PEG spacer arms The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini aspartate residues or glutamate residues using EDC Part No 22980 a water soluble carbodiimide crosslinker EDC activates carboxyl groups to bind to the NH2 group of the amino biotin forming an amide bond We manufacture biotin reagents to ensure the highest possible overall product integrity consistency and performance for the intended research applications Amino biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways The most common method is to crosslink the terminal primary amine to carboxyl groups using Carboxyl groups COOH occur in aspartate or glutamate residues and the carboxy terminus of polypeptides When activated with EDC Part No 22980 carboxylates react with amino NH2 groups to form amide bonds Carboxylate molecules and surface materials can be pre activated using EDC with Sulfo NHS Part No 24510 for subsequent reaction to primary amines see NHS ester Chemistry Related Products EZ Link Amine PEG2 Biotin
    https://www.bioz.com/result/amine peg3 biotin/product/Thermo Fisher
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    amine peg3 biotin - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond
    Article Snippet: .. Protein attached chromatin capture (PAtCh-Cap) for ChIP-exo Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min. .. These beads were selected as they have the same size and core material composition as the protein G coated magnetic beads utilized in the Active Motif ChIP-exo Kit.

    In Vitro:

    Article Title: Coating of Biomaterial Scaffolds with the Collagen-Mimetic Peptide GFOGER for Bone Defect Repair
    Article Snippet: .. For in vitro detection of GFOGER on PCL scaffolds, the carboxyl end of the GFOGER peptide was biotinylated using an EZ-Link® Amine-PEG3 -Biotin kit (Pierce Biotechnology), according to manufacturer’s instructions. .. Unreacted biotin was removed via overnight dialysis against PBS using a Slide-A-Lyzer Dialysis Cassette with a molecular weight cut-off of 3500 (Thermo Scientific).

    Synthesized:

    Article Title: Site-Specific Incorporation of Quadricyclane into a Protein and Photocleavage of the Quadricyclane Ligation Adduct
    Article Snippet: .. Dithiocarbonate S1 ( ) was synthesized according to the route developed by Sletten et al. EZ-link amine-PEG3 -biotin from Thermo Scientific (53 mg) was transferred to a round bottom flask as a solution in water, concentrated to a solid on a rotary evaporator, and azeotroped from toluene (2 × 2 mL). ..

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    Thermo Fisher ez link amine peg3 biotin
    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with <t>amine-PEG3-biotin</t> (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.
    Ez Link Amine Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez link amine peg3 biotin/product/Thermo Fisher
    Average 88 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ez link amine peg3 biotin - by Bioz Stars, 2020-09
    88/100 stars
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    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Journal: Nucleic Acids Research

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond

    doi: 10.1093/nar/gkw741

    Figure Lengend Snippet: Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Article Snippet: Protein attached chromatin capture (PAtCh-Cap) for ChIP-exo Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min.

    Techniques: Magnetic Beads, Isolation, Incubation, Immunoprecipitation