amine peg3 biotin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher amine peg3 biotin
    Amine Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amine peg3 biotin/product/Thermo Fisher
    Average 87 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    amine peg3 biotin - by Bioz Stars, 2020-01
    87/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II
    Article Snippet: Amine-PEG3-biotin was purchased from Thermo Fisher Scientific (Waltham, MA). .. All other chemicals were of high-performance liquid chromatography (HPLC) grade.

    Plasmid Preparation:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

    Magnetic Beads:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Biotin-labeled DNA was precipitated using Strepavidin coated magnetic beads (NEB), digested with NspI (NEB), and unbound fragments were removed by washing with wash Buffer (10 mM Tris HCl, 1mM EDTA, 2 M NaCl).

    Labeling:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: .. Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Biotin-labeled DNA was precipitated using Strepavidin coated magnetic beads (NEB), digested with NspI (NEB), and unbound fragments were removed by washing with wash Buffer (10 mM Tris HCl, 1mM EDTA, 2 M NaCl).

    Purification:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay).

    Real-time Polymerase Chain Reaction:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay).

    Polymerase Chain Reaction:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay).

    Incubation:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

    Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
    Article Snippet: Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific). .. The membrane was blocked with Odyssey blocking buffer (LI-COR Biosciences) with 1% SDS, incubated with IRDye Streptavidin 800CW, 1/10,000 (LI-COR Biosciences) in the same buffer, washed (three times in TBS-0.1% Tween20) and allowed to dry.

    Blocking Assay:

    Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
    Article Snippet: Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific). .. The membrane was blocked with Odyssey blocking buffer (LI-COR Biosciences) with 1% SDS, incubated with IRDye Streptavidin 800CW, 1/10,000 (LI-COR Biosciences) in the same buffer, washed (three times in TBS-0.1% Tween20) and allowed to dry.

    Modification:

    Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
    Article Snippet: Selective labelling of protein-blocked DNA ends The selective labelling of protein-blocked DNA ends (SLOPE) assay is a modification of selective labelling of TOP2 cleavage sites (SLOT) protocol . .. Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific).

    Lysis:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: SLOT assay Cells were lysed in SLOT lysis buffer (2% SDS, 10 mM EDTA in 10 mM Tris-HCl pH 8) and DNA was extracted after proteinase K digestion using the Qiagen Blood and Tissue Kit (Qiagen). .. Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific).

    Recombinant:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. To test the specificity of the SLOT assay, the pRYG plasmid, which contains a TOP2 cleavage and recognition site was incubated with recombinant TOP2A (TopoGen, Inc, Port Orange, FL) for 30 min at 37°C in the absence or presence of Etoposide (Sigma).

    Article Title: Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II
    Article Snippet: Chondroitin lyase AC, Chondroitin lyase ABC from Proteus vulgaris and recombinant Flavobacterial heparinase I, II, and III were expressed in E. coli in our laboratory. .. Amine-PEG3-biotin was purchased from Thermo Fisher Scientific (Waltham, MA).

    Chromatin Immunoprecipitation:

    Article Title: Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A
    Article Snippet: CS-E (2 mg) and amine-PEG3-Biotin (2 mg, Pierce, Rockford, IL) were dissolved in 200 µl H2 O and 10 mg NaCNBH3 was added to prepare biotinylated CS-E. .. Biotinylated CS-E was collected, freeze-dried and used for streptavidin (SA) chip preparation.

    SPR Assay:

    Article Title: Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A
    Article Snippet: Paragraph title: 2.6 Surface Plasma Resonance (SPR) analysis ... CS-E (2 mg) and amine-PEG3-Biotin (2 mg, Pierce, Rockford, IL) were dissolved in 200 µl H2 O and 10 mg NaCNBH3 was added to prepare biotinylated CS-E.

    Molecular Weight:

    Article Title: Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A
    Article Snippet: CS-E (2 mg) and amine-PEG3-Biotin (2 mg, Pierce, Rockford, IL) were dissolved in 200 µl H2 O and 10 mg NaCNBH3 was added to prepare biotinylated CS-E. .. The reaction mixture was heated at 70 °C for 24 h, after that a further 10 mg NaCNBH3 was added and the reaction was heated at 70 °C for another 24 h. After cooling to room temperature, the mixture was desalted with the spin column (3,000 molecular weight cut off (MWCO)).

    Software:

    Article Title: Semi-synthesis of chondroitin sulfate-E from chondroitin sulfate-A
    Article Snippet: CS-E (2 mg) and amine-PEG3-Biotin (2 mg, Pierce, Rockford, IL) were dissolved in 200 µl H2 O and 10 mg NaCNBH3 was added to prepare biotinylated CS-E. .. CS-E (2 mg) and amine-PEG3-Biotin (2 mg, Pierce, Rockford, IL) were dissolved in 200 µl H2 O and 10 mg NaCNBH3 was added to prepare biotinylated CS-E.

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    Thermo Fisher ez link amine peg3 biotin
    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with <t>amine-PEG3-biotin</t> (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.
    Ez Link Amine Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez link amine peg3 biotin/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ez link amine peg3 biotin - by Bioz Stars, 2020-01
    90/100 stars
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    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Journal: Nucleic Acids Research

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond

    doi: 10.1093/nar/gkw741

    Figure Lengend Snippet: Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Article Snippet: Protein attached chromatin capture (PAtCh-Cap) for ChIP-exo Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min.

    Techniques: Magnetic Beads, Isolation, Incubation, Immunoprecipitation