amine peg3 biotin  (Thermo Fisher)


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    Name:
    EZ-Link Amine-PEG3-Biotin
    Description:
    Thermo Scientific EZ-Link Amine-PEG3-Biotin is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods. Features of EZ-Link Amine-PEG3-Biotin: • Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins • Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers • Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules • Medium length—spacer arm (total length added to target) is 22.9 angstroms Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the—NH2 group of the amino-biotin, forming an amide bond. We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications. Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using . Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22980), carboxylates react with amino (—NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry). Related Products EZ-Link Amine-PEG2-Biotin
    Catalog Number:
    21347
    Price:
    None
    Applications:
    Protein Biology|Protein Labeling|Protein Labeling & Crosslinking
    Conjugate:
    Biotin
    Size:
    50 mg
    Category:
    Labeling & Detection Products, Labels & Labeling Kits, Aldehyde-, Ketone-, & Carboxylate-Reactive Labels
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher amine peg3 biotin
    Thermo Scientific EZ-Link Amine-PEG3-Biotin is a water-soluble biotin compounds containing a polyethylene glycol (PEG) spacer arm and a terminal primary amine for conjugation via EDC and other crosslinker methods. Features of EZ-Link Amine-PEG3-Biotin: • Biotinylation—label molecules and surfaces for assays or affinity purification methods involving avidin or streptavidin probes and resins • Amine-activated—primary amine can be crosslinked to proteins and material surfaces using EDC and other crosslinkers • Pegylated—polyethylene glycol (PEG) groups in spacer arm enhances water solubility of biotinylated molecules • Medium length—spacer arm (total length added to target) is 22.9 angstroms Amine-PEG2-Biotin and Amine-PEG3-Biotin are the shorter two of three amine-modified biotin compounds that contain polyethylene glycol (PEG) spacer arms. The short PEG segments are hydrophilic and confer greater solubility to labeled proteins compared to reagents having only hydrocarbon spacers. The primary amines of these pegylated biotin reagents can be conjugated to carboxyl groups on carboxy termini, aspartate residues or glutamate residues using EDC (Part No. 22980), a water-soluble carbodiimide crosslinker. EDC activates carboxyl groups to bind to the—NH2 group of the amino-biotin, forming an amide bond. We manufacture biotin reagents to ensure the highest possible overall product integrity, consistency and performance for the intended research applications. Amino-biotin compounds can be conjugated to functional groups of proteins and other molecules in a variety of ways. The most common method is to crosslink the terminal primary amine to carboxyl groups using . Carboxyl groups (-COOH) occur in aspartate or glutamate residues and the carboxy-terminus of polypeptides. When activated with EDC (Part No. 22980), carboxylates react with amino (—NH2) groups to form amide bonds. Carboxylate molecules and surface materials can be pre-activated using EDC with Sulfo-NHS (Part No. 24510) for subsequent reaction to primary amines (see NHS-ester Chemistry). Related Products EZ-Link Amine-PEG2-Biotin
    https://www.bioz.com/result/amine peg3 biotin/product/Thermo Fisher
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    amine peg3 biotin - by Bioz Stars, 2019-12
    83/100 stars

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    Related Articles

    High Performance Liquid Chromatography:

    Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
    Article Snippet: DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine. .. DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine.

    Flow Cytometry:

    Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
    Article Snippet: DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine. .. HPLC purification was performed on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2 – 100% B gradient for 98 min).

    Article Title: A modular IgG-scFv bispecific antibody topology
    Article Snippet: DOTA-biotin was synthesized by adding p-SCN-B n -DOTA ( S -2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid) purchased from Macrocyclics (Dallas, TX) to Amine-PEG3 -Biotin (Thermo Fisher Scientific) in dimethyl sulfoxide (DMSO) purchased from Sigma Aldrich (St. Louis, MO) with a 10-fold molar excess of triethylamine (TEA) purchased from VWR (West Chester, PA). .. The reaction mixture was vortexed at room temperature for 3 h, and purified by high-performance liquid chromatography (HPLC) on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2–100% B gradient for 98 min).

    Synthesized:

    Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
    Article Snippet: Stock solutions of DOTA and S-2-(4-Aminobenzyl)-1,4,7,10-tetraazacyclododecane tetraacetic acid (DOTA-Bn) where purchased from Macrocyclics (Dallas, TX) and dissolved in 0.4 M sodium acetate, pH 5.2. .. DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine. .. The reaction mixture was vortexed at room temperature for 3 h, and then purified by high performance liquid chromatography (HPLC).

    Article Title: A modular IgG-scFv bispecific antibody topology
    Article Snippet: Purified bsAb and IgG were analyzed by SDS-PAGE and quantified by absorbance at 280 nm. .. DOTA-biotin was synthesized by adding p-SCN-B n -DOTA ( S -2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid) purchased from Macrocyclics (Dallas, TX) to Amine-PEG3 -Biotin (Thermo Fisher Scientific) in dimethyl sulfoxide (DMSO) purchased from Sigma Aldrich (St. Louis, MO) with a 10-fold molar excess of triethylamine (TEA) purchased from VWR (West Chester, PA). .. The reaction mixture was vortexed at room temperature for 3 h, and purified by high-performance liquid chromatography (HPLC) on a C-18 reverse-phase column (Agilent Model 1100 HPLC, 1 × 25 cm, buffer A = 0.05% trifluoroacetic acid (TFA), buffer B = 0.0425% TFA in 80% acetonitrile, 2–100% B gradient for 98 min).

    Labeling:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Cells were lysed in SLOT lysis buffer (2% SDS, 10 mM EDTA in 10 mM Tris-HCl pH 8) and DNA was extracted after proteinase K digestion using the Qiagen Blood and Tissue Kit (Qiagen). .. Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Biotin-labeled DNA was precipitated using Strepavidin coated magnetic beads (NEB), digested with NspI (NEB), and unbound fragments were removed by washing with wash Buffer (10 mM Tris HCl, 1mM EDTA, 2 M NaCl).

    Purification:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Biotin-labeled DNA was precipitated using Strepavidin coated magnetic beads (NEB), digested with NspI (NEB), and unbound fragments were removed by washing with wash Buffer (10 mM Tris HCl, 1mM EDTA, 2 M NaCl).

    Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
    Article Snippet: DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine. .. DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine.

    Real-time Polymerase Chain Reaction:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Biotin-labeled DNA was precipitated using Strepavidin coated magnetic beads (NEB), digested with NspI (NEB), and unbound fragments were removed by washing with wash Buffer (10 mM Tris HCl, 1mM EDTA, 2 M NaCl).

    Polymerase Chain Reaction:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Biotin-labeled DNA was precipitated using Strepavidin coated magnetic beads (NEB), digested with NspI (NEB), and unbound fragments were removed by washing with wash Buffer (10 mM Tris HCl, 1mM EDTA, 2 M NaCl).

    Incubation:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay).

    Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
    Article Snippet: Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific). .. Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific).

    Blocking Assay:

    Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
    Article Snippet: Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific). .. Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific).

    Mass Spectrometry:

    Article Title: Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging
    Article Snippet: DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine. .. DOTA-Bn-biotin ( ) was synthesized by dissolving Amine-PEG3 -Biotin purchased from Thermo Scientific (Rockford, IL) and S-2-(4-Isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid (p-SCN-Bn-DOTA) purchased from Macrocyclics in dimethyl sulfoxide (DMSO) with a 10 fold molar excess of triethylamine.

    Article Title: A modular IgG-scFv bispecific antibody topology
    Article Snippet: DOTA-biotin was synthesized by adding p-SCN-B n -DOTA ( S -2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid) purchased from Macrocyclics (Dallas, TX) to Amine-PEG3 -Biotin (Thermo Fisher Scientific) in dimethyl sulfoxide (DMSO) purchased from Sigma Aldrich (St. Louis, MO) with a 10-fold molar excess of triethylamine (TEA) purchased from VWR (West Chester, PA). .. DOTA-biotin was synthesized by adding p-SCN-B n -DOTA ( S -2-(4-isothiocyanatobenzyl)-1,4,7,10-tetraazacyclododecane-tetraacetic acid) purchased from Macrocyclics (Dallas, TX) to Amine-PEG3 -Biotin (Thermo Fisher Scientific) in dimethyl sulfoxide (DMSO) purchased from Sigma Aldrich (St. Louis, MO) with a 10-fold molar excess of triethylamine (TEA) purchased from VWR (West Chester, PA).

    Modification:

    Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
    Article Snippet: The selective labelling of protein-blocked DNA ends (SLOPE) assay is a modification of selective labelling of TOP2 cleavage sites (SLOT) protocol . .. Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific).

    Lysis:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Cells were lysed in SLOT lysis buffer (2% SDS, 10 mM EDTA in 10 mM Tris-HCl pH 8) and DNA was extracted after proteinase K digestion using the Qiagen Blood and Tissue Kit (Qiagen). .. Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific).

    Recombinant:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay).

    Article Title: Glycosaminoglycans from bovine eye vitreous humour and interaction with collagen type II
    Article Snippet: Chondroitin lyase AC, Chondroitin lyase ABC from Proteus vulgaris and recombinant Flavobacterial heparinase I, II, and III were expressed in E. coli in our laboratory. .. Amine-PEG3-biotin was purchased from Thermo Fisher Scientific (Waltham, MA).

    Plasmid Preparation:

    Article Title: Androgen-induced TOP2B mediated double strand breaks and prostate cancer gene rearrangements
    Article Snippet: Tyrosine residues covalently linked to DNA were labeled using EDC and Amine-PEG3-Biotin (Thermo Scientific). .. Precipitated DNA was eluted in 95% formamide, 10 mM EDTA for 5 min at 65°C, purified using the Qiagen PCR purification Kit (Qiagen) and analyzed by real-time PCR. (See for an overview of the SLOT assay).

    Software:

    Article Title: ATM specifically mediates repair of double-strand breaks with blocked DNA ends
    Article Snippet: Tyrosine residues that remain covalently linked to DNA were labelled with biotin using EDC and amine-PEG3-biotin (Thermo Scientific). .. The membrane was blocked with Odyssey blocking buffer (LI-COR Biosciences) with 1% SDS, incubated with IRDye Streptavidin 800CW, 1/10,000 (LI-COR Biosciences) in the same buffer, washed (three times in TBS-0.1% Tween20) and allowed to dry.

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  • 79
    Thermo Fisher ez link amine peg3 biotin
    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with <t>amine-PEG3-biotin</t> (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.
    Ez Link Amine Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ez link amine peg3 biotin/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ez link amine peg3 biotin - by Bioz Stars, 2019-12
    79/100 stars
      Buy from Supplier

    83
    Thermo Fisher amine peg3 biotin
    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with <t>amine-PEG3-biotin</t> (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.
    Amine Peg3 Biotin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 83/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amine peg3 biotin/product/Thermo Fisher
    Average 83 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    amine peg3 biotin - by Bioz Stars, 2019-12
    83/100 stars
      Buy from Supplier

    Image Search Results


    Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Journal: Nucleic Acids Research

    Article Title: PAtCh-Cap: input strategy for improving analysis of ChIP-exo data sets and beyond

    doi: 10.1093/nar/gkw741

    Figure Lengend Snippet: Schematic overview for the protein attached chromatin capture (PAtCh-Cap) method. Streptavidin coated magnetic beads are first conjugated with amine-PEG3-biotin (where PEG is polyethylene glycol). After standard cross-linking, chromatin isolation and DNA shearing procedures, 10% of the sample volume is removed and incubated with the pre-conjugated beads in the presence of EDC (1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride). EDC reacts with protein carboxylate groups, forming unstable o -acylisourea activated esters that can undergo nucleophilic attack by the primary amines on the amine-PEG3-biotin prosthetics. This forms a covalent amide linkage with proteins in the chromatin complexes and releases an isourea by-product. Once bead-bound, these chromatin complexes can be subjected to additional chemical processing steps in parallel with bead-bound chromatin that was separately immunoprecipitated for the target of interest.

    Article Snippet: Following two series of washes with 0.01 M PBS (pH 7.4), 50 μg of M-280 streptavidin coated Dynabeads (Thermo Fisher Scientific; equivalent to the number of beads utilized per reaction in the Active Motif ChIP-exo Kit) were conjugated with 10 μl of 50 nM EZ-link amine-PEG3-Biotin (Thermo Fisher Scientific; PEG = polyethylene glycol) in 0.01 M PBS (pH 7.4) at room temperature for 20 min.

    Techniques: Magnetic Beads, Isolation, Incubation, Immunoprecipitation