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    Structured Review

    Millipore amicon ultra centrifugal filter
    Amicon Ultra Centrifugal Filter, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amicon ultra centrifugal filter/product/Millipore
    Average 99 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    amicon ultra centrifugal filter - by Bioz Stars, 2020-01
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Paragraph title: Cloning, expression and purification of recombinant proteins ... The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Centrifugation:

    Article Title: Rapid Proteome Changes in Plasma and Cerebrospinal Fluid Following Bacterial Infection in Preterm Newborn Pigs
    Article Snippet: CSF or a plasma sample containing 100 μg protein was transferred onto an Amicon Ultra centrifugal filter (10 kDa, 0.5 mL, Millipore, Søborg, Denmark) and mixed with a buffer containing sodium deoxycholate (5%) and triethylammonium bicarbonate (50 mmol/L, pH 8.0). .. Tryptic peptides were recovered by another step of centrifugation and purified by phase extraction using ethyl acetate acidified by trifluoroacetic acid (1%, v/v).

    Article Title: Construction of a Robust Cofactor Self-Sufficient Bienzyme Biocatalytic System for Dye Decolorization and its Mathematical Modeling
    Article Snippet: The recombinant cells were collected by centrifugation at 10,000× g at 4 °C for 10 min, followed by ultrasonic decomposition and nickel-chelating affinity chromatography purification. .. The eluent was desalted and concentrated with 25 mM sodium phosphate buffer (pH 7.0) containing 10% glycerol using Amicon Ultra Centrifugal Filter (Millipore, Billerica, MA, USA) at 7500× g for 30 min. All purification procedures were implemented at 4 °C.

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: Soluble fraction of each lysate was obtained by centrifugation at 15,000 × g for 20 min at 4°C. .. The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore).

    Amplification:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: After digestion with Nhe I and either Xho I or Bam HI, the amplified cDNAs were cloned into the modified episomal expression vector pCEP-Pu-double strep II-tag (N- or C-terminal) in-frame with the 5′ sequence of the BM-40 signal peptide and confirmed by Sanger nucleotide sequencing. .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Filtration:

    Article Title: Qualitative microbiome profiling along a wastewater system in Kampala, Uganda
    Article Snippet: Concentration was performed using a benchtop tangential flow filtration unit (Natan GmbH; Zurich, Switzerland) and UP020 20 kDa filter membranes (Microdyn-Nadir; Wiesbaden, Germany) into 50 ml sterile tubes. .. Upon arrival in Switzerland, the samples were further concentrated with Amicon Ultra Centrifugal Filter Units with a molecular cut-off of 10 kDa (Millipore; Billerica, MA, USA) into a smaller volume of approximately 250–300 µl.

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Briefly, buffer exchange of protein samples to 100 mM NaHCO3, 100 mM NaCl (pH 8.3) was performed using Amicon ultra centrifugal filter unit (Millipore). .. Free dye was then separated using gel filtration by loading the protein/dye reaction mixture onto a 15-ml self-packed Sephadex G-25 (Sigma) column and elution with PBS, pH 7.4.

    Mass Spectrometry:

    Article Title: Rapid Proteome Changes in Plasma and Cerebrospinal Fluid Following Bacterial Infection in Preterm Newborn Pigs
    Article Snippet: Paragraph title: Mass Spectrometry (MS)-Based Proteomics ... CSF or a plasma sample containing 100 μg protein was transferred onto an Amicon Ultra centrifugal filter (10 kDa, 0.5 mL, Millipore, Søborg, Denmark) and mixed with a buffer containing sodium deoxycholate (5%) and triethylammonium bicarbonate (50 mmol/L, pH 8.0).

    Molecular Cloning:

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: Paragraph title: Molecular cloning, recombinant expression and purification of MAGIC Factor ... The purified protein was then concentrated into 1.5x PBS (pH 7.4), 1 mM TCEP and 10% Glycerol using Amicon Ultra centrifugal filter units (EMD Millipore) and stored at −80°C in aliquots.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions
    Article Snippet: Eluted IgG (Protein G) and flow through (Melon Gel) were concentrated, and buffer exchanged into PBS using a 30 kDa Amicon Ultra centrifugal filter (Millipore, USA). .. IgG concentrations were quantitated using the Human-IgG ELISA kit (Mabtech, Sweden).

    Incubation:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Transfected cells were cultured in DMEM medium supplemented with 10% FCS (Gibco), penicillin (1000 U/ml)/streptomycin (1000 µg/ml), and puromycin (3 µg/ml, Gold Biotechnology) for positive selection and were incubated at 37 °C/5% CO2 to allow growth to 100% confluency as adherent monolayers in cell culture triple-flasks (NuncTM , Thermo Fisher Scientific). .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Article Title: Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions
    Article Snippet: After 5 min incubation, the purified IgG was collected in the flow through following the manufacturer's instructions. .. Eluted IgG (Protein G) and flow through (Melon Gel) were concentrated, and buffer exchanged into PBS using a 30 kDa Amicon Ultra centrifugal filter (Millipore, USA).

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: .. Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label. ..

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Briefly, buffer exchange of protein samples to 100 mM NaHCO3, 100 mM NaCl (pH 8.3) was performed using Amicon ultra centrifugal filter unit (Millipore). .. Protein sample was then incubated with a five-fold amount of Alexa Fluor 555 succinimidyl ester (stock dissolved at 10 mg/ml in dry dimethylformamide) for 1 h at 25 °C followed by addition of 10% (v/v) hydroxylamine (1.5 M), pH 8.5, and incubation of the hydroxylamine-containing reaction mixture for 1 h to terminate the reaction.

    Expressing:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Paragraph title: Cloning, expression and purification of recombinant proteins ... The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: Paragraph title: Molecular cloning, recombinant expression and purification of MAGIC Factor ... The purified protein was then concentrated into 1.5x PBS (pH 7.4), 1 mM TCEP and 10% Glycerol using Amicon Ultra centrifugal filter units (EMD Millipore) and stored at −80°C in aliquots.

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: All expressions were performed at 20°C for at least 18 h except for the expression of LDH, which was performed at 37°C for 4 h. Cells were harvested by centrifugation at 5,000 × g for 20 min at 4°C. .. The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore).

    BIA-KA:

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore). .. Protein concentrations were determined using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, USA).

    Modification:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: After digestion with Nhe I and either Xho I or Bam HI, the amplified cDNAs were cloned into the modified episomal expression vector pCEP-Pu-double strep II-tag (N- or C-terminal) in-frame with the 5′ sequence of the BM-40 signal peptide and confirmed by Sanger nucleotide sequencing. .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Derivative Assay:

    Article Title: Data on the preparation of chitosan-tripolyphosphate nanoparticles and its entrapment mechanism for egg white derived peptides
    Article Snippet: Paragraph title: Entrapment of egg white derived peptides (EWDP) in CS-TPP NPs ... The excess EWDP was separated at 4000 r/min for 10 min with an Amicon Ultra centrifugal filter (10 k MWCO, Millipore Corp.).

    Gel Purification:

    Article Title: Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions
    Article Snippet: IgG was purified from plasma samples by Melon Gel purification according to the manufacturer's protocol. .. Eluted IgG (Protein G) and flow through (Melon Gel) were concentrated, and buffer exchanged into PBS using a 30 kDa Amicon Ultra centrifugal filter (Millipore, USA).

    High Performance Liquid Chromatography:

    Article Title: Development of a topical liposomal formulation of Amphotericin B for the treatment of cutaneous leishmaniasis
    Article Snippet: .. 2.4 Encapsulation efficiency of AmB in liposomes Liposomes containing AmB were diluted 1 to 10, and were transferred into a 15 mL Amicon Ultra centrifugal filter unit (with a cut off 100 kDa, Sigma, USA) and centrifuged at 4,000 × g for 30 min, then, the filtrate was analyzed for AmB using HPLC. .. 2.5 In vitro effect of Lip-AmB on L. major promastigote and amastigote growth The effect of Lip-AmB formulations on L. major promastigotes (MRHO/IR/75/ER) and amastigotes was assessed and compared with Fungizone.

    Transfection:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Transfected cells were cultured in DMEM medium supplemented with 10% FCS (Gibco), penicillin (1000 U/ml)/streptomycin (1000 µg/ml), and puromycin (3 µg/ml, Gold Biotechnology) for positive selection and were incubated at 37 °C/5% CO2 to allow growth to 100% confluency as adherent monolayers in cell culture triple-flasks (NuncTM , Thermo Fisher Scientific). .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Chromatography:

    Article Title: Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions
    Article Snippet: IgG Purification IgG was purified from plasma samples from healthy individuals, or from HIV positive individuals , via Protein G chromatography (Protein G HP Multitrap, GE Healthcare, Sweden) and Melon Gel chromatography (Melon Gel IgG Purification Kit, Thermo Fisher Scientific, USA). .. Eluted IgG (Protein G) and flow through (Melon Gel) were concentrated, and buffer exchanged into PBS using a 30 kDa Amicon Ultra centrifugal filter (Millipore, USA).

    Protease Inhibitor:

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: The cell pellet was frozen overnight and all proteins extracted using 2x CellLytic B reagent (Sigma Aldrich) adjusted to pH 7.2, 0.2 mg/ml Lysozyme, Nuclease (Thermo Scientific) and 2x EDTA-free HALT protease inhibitor cocktail (Thermo Scientific) for 30 min at 4°C. .. The purified protein was then concentrated into 1.5x PBS (pH 7.4), 1 mM TCEP and 10% Glycerol using Amicon Ultra centrifugal filter units (EMD Millipore) and stored at −80°C in aliquots.

    Buffer Exchange:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: .. Briefly, buffer exchange of protein samples to 100 mM NaHCO3, 100 mM NaCl (pH 8.3) was performed using Amicon ultra centrifugal filter unit (Millipore). .. Protein sample was then incubated with a five-fold amount of Alexa Fluor 555 succinimidyl ester (stock dissolved at 10 mg/ml in dry dimethylformamide) for 1 h at 25 °C followed by addition of 10% (v/v) hydroxylamine (1.5 M), pH 8.5, and incubation of the hydroxylamine-containing reaction mixture for 1 h to terminate the reaction.

    Cell Culture:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Cell culture supernatants containing the secreted target proteins were collected and supplemented with phenylmethylsulfonyl fluoride (1 mM, Applichem) before passing over self-packed streptactin-sepharose columns (0.5 ml, IBA). .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Article Title: Construction of a Robust Cofactor Self-Sufficient Bienzyme Biocatalytic System for Dye Decolorization and its Mathematical Modeling
    Article Snippet: Preparation of Recombinant Enzymes The recombinant cells were cultured in a 500 mL flask containing 100 mL of Luria-Bertani (LB) medium at 37 °C, with 50 μg/ml of kanamycin added. .. The eluent was desalted and concentrated with 25 mM sodium phosphate buffer (pH 7.0) containing 10% glycerol using Amicon Ultra Centrifugal Filter (Millipore, Billerica, MA, USA) at 7500× g for 30 min. All purification procedures were implemented at 4 °C.

    Protein Concentration:

    Article Title: Rapid Proteome Changes in Plasma and Cerebrospinal Fluid Following Bacterial Infection in Preterm Newborn Pigs
    Article Snippet: Protein concentration was determined by the absorbance at 280 nm on a NanoDrop Spectrophotometer (Thermo Scientific, Waltham, MA, USA) with bovine serum albumin (BSA) as a standard. .. CSF or a plasma sample containing 100 μg protein was transferred onto an Amicon Ultra centrifugal filter (10 kDa, 0.5 mL, Millipore, Søborg, Denmark) and mixed with a buffer containing sodium deoxycholate (5%) and triethylammonium bicarbonate (50 mmol/L, pH 8.0).

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: The purified protein was then concentrated into 1.5x PBS (pH 7.4), 1 mM TCEP and 10% Glycerol using Amicon Ultra centrifugal filter units (EMD Millipore) and stored at −80°C in aliquots. .. Generally, protein concentration and degree of labeling (see below) were determined using a NanoDrop device (Thermo Scientific).

    Article Title: Construction of a Robust Cofactor Self-Sufficient Bienzyme Biocatalytic System for Dye Decolorization and its Mathematical Modeling
    Article Snippet: The eluent was desalted and concentrated with 25 mM sodium phosphate buffer (pH 7.0) containing 10% glycerol using Amicon Ultra Centrifugal Filter (Millipore, Billerica, MA, USA) at 7500× g for 30 min. All purification procedures were implemented at 4 °C. .. The protein concentration was determined by Bradford’s method using bovine serum albumin as the standard.

    Sequencing:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: After digestion with Nhe I and either Xho I or Bam HI, the amplified cDNAs were cloned into the modified episomal expression vector pCEP-Pu-double strep II-tag (N- or C-terminal) in-frame with the 5′ sequence of the BM-40 signal peptide and confirmed by Sanger nucleotide sequencing. .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Affinity Purification:

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: Labeled protein was purified twice into 25 mM HEPES pH 7.2, 150 mM NaCl, 1 mM TCEP, 1 mM EDTA, 10% Glycerol using Zeba Spin Desalting Columns (Thermo Scientific) and concentrated using Amicon Ultra centrifugal filter units (EMD Millipore). .. For affinity purification of labeled MAGIC Factor (see below), the protein was purified twice into EMSA buffer (see below).

    Binding Assay:

    Article Title: Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions
    Article Snippet: For Protein G sepharose purification, plasma was diluted 2-fold with binding buffer (20 mM sodium phosphate, pH 7.0) and incubated for 1 h. IgG was eluted with 0.1 M glycine-HCl (pH 2.7), into neutralizing buffer (1 M Tris-HCl, pH 9.0). .. Eluted IgG (Protein G) and flow through (Melon Gel) were concentrated, and buffer exchanged into PBS using a 30 kDa Amicon Ultra centrifugal filter (Millipore, USA).

    Molecular Weight:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use. .. Coomassie stained gels and immunoblotting analysis For protein analysis by Coomassie staining, the purified TSPs were separated on 0.5% (w/v) agarose/3% (w/v) polyacrylamide composite gels without prior DTT treatment, while α2 δ-1S NTST and α2 NTST variants were separated using conventional SDS-PAGE on 7% gels in presence and absence of DTT.

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: .. Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label. ..

    DNA Extraction:

    Article Title: Qualitative microbiome profiling along a wastewater system in Kampala, Uganda
    Article Snippet: Upon arrival in Switzerland, the samples were further concentrated with Amicon Ultra Centrifugal Filter Units with a molecular cut-off of 10 kDa (Millipore; Billerica, MA, USA) into a smaller volume of approximately 250–300 µl. .. Nucleic acids were isolated from 250 µl of the concentrated samples using a PowerSoil DNA isolation kit (MO-BIO; Carlsbad, CA, USA) following the manufacturer’s instruction except for the elution step which was done in 60 µl purified water.

    Nucleic Acid Electrophoresis:

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore). .. Purity and size of the enzymes were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie staining.

    Fluorescence:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Paragraph title: Fluorescence labelling of TSP-4 ... Briefly, buffer exchange of protein samples to 100 mM NaHCO3, 100 mM NaCl (pH 8.3) was performed using Amicon ultra centrifugal filter unit (Millipore).

    Mutagenesis:

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: However, the single mutant PPARγQ322C was labeled with a twofold molar excess of BTFA following drug addition with the following exceptions, samples loaded with the covalent ligands were labeled with a tenfold molar excess of BTFA while the apo sample was labeled with a stoichiometric concentration of BTFA to reduce labeling on the native cysteine in the ligand binding pocket. .. Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label.

    Isolation:

    Article Title: Qualitative microbiome profiling along a wastewater system in Kampala, Uganda
    Article Snippet: Upon arrival in Switzerland, the samples were further concentrated with Amicon Ultra Centrifugal Filter Units with a molecular cut-off of 10 kDa (Millipore; Billerica, MA, USA) into a smaller volume of approximately 250–300 µl. .. Nucleic acids were isolated from 250 µl of the concentrated samples using a PowerSoil DNA isolation kit (MO-BIO; Carlsbad, CA, USA) following the manufacturer’s instruction except for the elution step which was done in 60 µl purified water.

    Flow Cytometry:

    Article Title: Qualitative microbiome profiling along a wastewater system in Kampala, Uganda
    Article Snippet: Concentration was performed using a benchtop tangential flow filtration unit (Natan GmbH; Zurich, Switzerland) and UP020 20 kDa filter membranes (Microdyn-Nadir; Wiesbaden, Germany) into 50 ml sterile tubes. .. Upon arrival in Switzerland, the samples were further concentrated with Amicon Ultra Centrifugal Filter Units with a molecular cut-off of 10 kDa (Millipore; Billerica, MA, USA) into a smaller volume of approximately 250–300 µl.

    Article Title: Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions
    Article Snippet: .. Eluted IgG (Protein G) and flow through (Melon Gel) were concentrated, and buffer exchanged into PBS using a 30 kDa Amicon Ultra centrifugal filter (Millipore, USA). .. IgG concentrations were quantitated using the Human-IgG ELISA kit (Mabtech, Sweden).

    Labeling:

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: .. Labeled protein was purified twice into 25 mM HEPES pH 7.2, 150 mM NaCl, 1 mM TCEP, 1 mM EDTA, 10% Glycerol using Zeba Spin Desalting Columns (Thermo Scientific) and concentrated using Amicon Ultra centrifugal filter units (EMD Millipore). .. Generally, MAGIC Factor was labeled at a 3:1 dye:protein ratio, except for experiments where a range of dye:protein ratio was investigated.

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: The purified protein was then concentrated into 1.5x PBS (pH 7.4), 1 mM TCEP and 10% Glycerol using Amicon Ultra centrifugal filter units (EMD Millipore) and stored at −80°C in aliquots. .. Generally, protein concentration and degree of labeling (see below) were determined using a NanoDrop device (Thermo Scientific).

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: .. Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label. ..

    Purification:

    Article Title: Rapid Proteome Changes in Plasma and Cerebrospinal Fluid Following Bacterial Infection in Preterm Newborn Pigs
    Article Snippet: CSF or a plasma sample containing 100 μg protein was transferred onto an Amicon Ultra centrifugal filter (10 kDa, 0.5 mL, Millipore, Søborg, Denmark) and mixed with a buffer containing sodium deoxycholate (5%) and triethylammonium bicarbonate (50 mmol/L, pH 8.0). .. Tryptic peptides were recovered by another step of centrifugation and purified by phase extraction using ethyl acetate acidified by trifluoroacetic acid (1%, v/v).

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: .. Labeled protein was purified twice into 25 mM HEPES pH 7.2, 150 mM NaCl, 1 mM TCEP, 1 mM EDTA, 10% Glycerol using Zeba Spin Desalting Columns (Thermo Scientific) and concentrated using Amicon Ultra centrifugal filter units (EMD Millipore). .. Generally, MAGIC Factor was labeled at a 3:1 dye:protein ratio, except for experiments where a range of dye:protein ratio was investigated.

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Paragraph title: Cloning, expression and purification of recombinant proteins ... The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Article Title: Qualitative microbiome profiling along a wastewater system in Kampala, Uganda
    Article Snippet: Upon arrival in Switzerland, the samples were further concentrated with Amicon Ultra Centrifugal Filter Units with a molecular cut-off of 10 kDa (Millipore; Billerica, MA, USA) into a smaller volume of approximately 250–300 µl. .. Nucleic acids were isolated from 250 µl of the concentrated samples using a PowerSoil DNA isolation kit (MO-BIO; Carlsbad, CA, USA) following the manufacturer’s instruction except for the elution step which was done in 60 µl purified water.

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: .. The purified protein was then concentrated into 1.5x PBS (pH 7.4), 1 mM TCEP and 10% Glycerol using Amicon Ultra centrifugal filter units (EMD Millipore) and stored at −80°C in aliquots. .. Generally, protein concentration and degree of labeling (see below) were determined using a NanoDrop device (Thermo Scientific).

    Article Title: Low pH Exposure During Immunoglobulin G Purification Methods Results in Aggregates That Avidly Bind Fcγ Receptors: Implications for Measuring Fc Dependent Antibody Functions
    Article Snippet: Paragraph title: IgG Purification ... Eluted IgG (Protein G) and flow through (Melon Gel) were concentrated, and buffer exchanged into PBS using a 30 kDa Amicon Ultra centrifugal filter (Millipore, USA).

    Article Title: Construction of a Robust Cofactor Self-Sufficient Bienzyme Biocatalytic System for Dye Decolorization and its Mathematical Modeling
    Article Snippet: .. The eluent was desalted and concentrated with 25 mM sodium phosphate buffer (pH 7.0) containing 10% glycerol using Amicon Ultra Centrifugal Filter (Millipore, Billerica, MA, USA) at 7500× g for 30 min. All purification procedures were implemented at 4 °C. .. The protein concentration was determined by Bradford’s method using bovine serum albumin as the standard.

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: In the case of PPARγC313A,Q322C the protein was labeled with a tenfold excess of BTFA during purification. .. Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label.

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: Paragraph title: Production and Purification of Recombinant Proteins ... The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore).

    Article Title: Characterization of a Novel Bispecific Antibody That Activates T Cells In Vitro and Slows Tumor Growth In Vivo
    Article Snippet: .. Fractions containing purified monomeric protein were pooled and concentrated using Amicon Ultra Centrifugal filter units (EMD Millipore, Billerica, MA). .. Bispecific antibody production Bispecific antibodies were produced from purified parental proteins using controlled Fab-arm exchange (cFAE) according to the established methods. ( , ) In a mouse framework, the F405L mutation on the heavy chain of one parental antibody and the T370K, K409R mutations on the heavy chain of another parental antibody permit efficient cFAE. CD3xEpCAM and CD3xnull were produced by mixing purified 2C11 mIgG2a sigma F405L with a 5% excess of purified anti-EpCAM or B21M mIgG2a sigma T370K/K409R; a 5% excess was used limit the presence of any residual bivalent anti-CD3ɛ.

    Affinity Chromatography:

    Article Title: Construction of a Robust Cofactor Self-Sufficient Bienzyme Biocatalytic System for Dye Decolorization and its Mathematical Modeling
    Article Snippet: The recombinant cells were collected by centrifugation at 10,000× g at 4 °C for 10 min, followed by ultrasonic decomposition and nickel-chelating affinity chromatography purification. .. The eluent was desalted and concentrated with 25 mM sodium phosphate buffer (pH 7.0) containing 10% glycerol using Amicon Ultra Centrifugal Filter (Millipore, Billerica, MA, USA) at 7500× g for 30 min. All purification procedures were implemented at 4 °C.

    Affinity Column:

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: The final soluble fractions containing the His-tagged proteins were loaded onto a 5 ml Ni-NTA affinity column (GE Healthcare) previously equilibrated with buffer containing 50 mM sodium phosphate buffer (NaP), 300 mM NaCl, and 20 mM imidazole, pH 8.0. .. The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore).

    SDS Page:

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore). .. Purity and size of the enzymes were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie staining.

    Plasmid Preparation:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: After digestion with Nhe I and either Xho I or Bam HI, the amplified cDNAs were cloned into the modified episomal expression vector pCEP-Pu-double strep II-tag (N- or C-terminal) in-frame with the 5′ sequence of the BM-40 signal peptide and confirmed by Sanger nucleotide sequencing. .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Article Title: Characterization of a Novel Bispecific Antibody That Activates T Cells In Vitro and Slows Tumor Growth In Vivo
    Article Snippet: Proteins were expressed by transfecting Expi293F cells with heavy and light chain plasmid at a ratio of 1:3, respectively, using an ExpiFectamine Kit according to the manufacturer's recommendations (Thermo Fisher Scientific, Carlsbad, CA). .. Fractions containing purified monomeric protein were pooled and concentrated using Amicon Ultra Centrifugal filter units (EMD Millipore, Billerica, MA).

    Recombinant:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use. .. Coomassie stained gels and immunoblotting analysis For protein analysis by Coomassie staining, the purified TSPs were separated on 0.5% (w/v) agarose/3% (w/v) polyacrylamide composite gels without prior DTT treatment, while α2 δ-1S NTST and α2 NTST variants were separated using conventional SDS-PAGE on 7% gels in presence and absence of DTT.

    Article Title: Multiplex live single-cell transcriptional analysis demarcates cellular functional heterogeneity
    Article Snippet: Paragraph title: Molecular cloning, recombinant expression and purification of MAGIC Factor ... The purified protein was then concentrated into 1.5x PBS (pH 7.4), 1 mM TCEP and 10% Glycerol using Amicon Ultra centrifugal filter units (EMD Millipore) and stored at −80°C in aliquots.

    Article Title: Construction of a Robust Cofactor Self-Sufficient Bienzyme Biocatalytic System for Dye Decolorization and its Mathematical Modeling
    Article Snippet: Paragraph title: 3.2. Preparation of Recombinant Enzymes ... The eluent was desalted and concentrated with 25 mM sodium phosphate buffer (pH 7.0) containing 10% glycerol using Amicon Ultra Centrifugal Filter (Millipore, Billerica, MA, USA) at 7500× g for 30 min. All purification procedures were implemented at 4 °C.

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: Paragraph title: Production and Purification of Recombinant Proteins ... The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore).

    Sample Prep:

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label. .. Following sample preparation 10% of final volume of buffered D2 O was added to all samples.

    Selection:

    Article Title: Direct, gabapentin-insensitive interaction of a soluble form of the calcium channel subunit α2δ-1 with thrombospondin-4
    Article Snippet: Transfected cells were cultured in DMEM medium supplemented with 10% FCS (Gibco), penicillin (1000 U/ml)/streptomycin (1000 µg/ml), and puromycin (3 µg/ml, Gold Biotechnology) for positive selection and were incubated at 37 °C/5% CO2 to allow growth to 100% confluency as adherent monolayers in cell culture triple-flasks (NuncTM , Thermo Fisher Scientific). .. The recombinant double strep II-tagged proteins were eluted with phosphate-buffered saline (PBS), pH 7.4 containing D-desthiobiotin (2.5 mM, Sigma), concentrated with Amicon ultra centrifugal filter units with molecular weight cutoff of 100 kDa for all recombinant proteins (Millipore), aliquoted after addition of glycerol (10% (v/v)) and stored at −80 °C until use.

    Homogenization:

    Article Title: Anemia in infancy is associated with alterations in systemic metabolism and microbial structure and function in a sex-specific manner: an observational study
    Article Snippet: After manual homogenization with a sterile microspatula (Corning), ∼250 mg fecal material was weighed, taking care to avoid any undigested food that may be present. .. The supernatant was subjected to 2 filtering steps, first through a 0.22-μm Millex GP PES membrane syringe filter (Millipore) and then through a 0.5-mL Amicon Ultra centrifugal filter (3000-MW cutoff; Millipore) at 14,000 x g for 45 min at 4°C.

    Nuclear Magnetic Resonance:

    Article Title: Anemia in infancy is associated with alterations in systemic metabolism and microbial structure and function in a sex-specific manner: an observational study
    Article Snippet: The supernatant was subjected to 2 filtering steps, first through a 0.22-μm Millex GP PES membrane syringe filter (Millipore) and then through a 0.5-mL Amicon Ultra centrifugal filter (3000-MW cutoff; Millipore) at 14,000 x g for 45 min at 4°C. .. A total of 180 μL was transferred to a 3-mm NMR tube (Bruker) and stored at 4°C until spectral acquisition.

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: Paragraph title: Preparation of NMR samples ... Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label.

    Spectrophotometry:

    Article Title: Rapid Proteome Changes in Plasma and Cerebrospinal Fluid Following Bacterial Infection in Preterm Newborn Pigs
    Article Snippet: Protein concentration was determined by the absorbance at 280 nm on a NanoDrop Spectrophotometer (Thermo Scientific, Waltham, MA, USA) with bovine serum albumin (BSA) as a standard. .. CSF or a plasma sample containing 100 μg protein was transferred onto an Amicon Ultra centrifugal filter (10 kDa, 0.5 mL, Millipore, Søborg, Denmark) and mixed with a buffer containing sodium deoxycholate (5%) and triethylammonium bicarbonate (50 mmol/L, pH 8.0).

    Article Title: Data on the preparation of chitosan-tripolyphosphate nanoparticles and its entrapment mechanism for egg white derived peptides
    Article Snippet: The excess EWDP was separated at 4000 r/min for 10 min with an Amicon Ultra centrifugal filter (10 k MWCO, Millipore Corp.). .. The unencapsulated EWDP in the filtrate was quantified with an established standard curve at 220 nm (R2 ≥ 0.999) recording from a UV–Vis spectrophotometer (UV-2550, Shimadzu, Tokyo, Japan).

    Concentration Assay:

    Article Title: Qualitative microbiome profiling along a wastewater system in Kampala, Uganda
    Article Snippet: Concentration was performed using a benchtop tangential flow filtration unit (Natan GmbH; Zurich, Switzerland) and UP020 20 kDa filter membranes (Microdyn-Nadir; Wiesbaden, Germany) into 50 ml sterile tubes. .. Upon arrival in Switzerland, the samples were further concentrated with Amicon Ultra Centrifugal Filter Units with a molecular cut-off of 10 kDa (Millipore; Billerica, MA, USA) into a smaller volume of approximately 250–300 µl.

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: However, the single mutant PPARγQ322C was labeled with a twofold molar excess of BTFA following drug addition with the following exceptions, samples loaded with the covalent ligands were labeled with a tenfold molar excess of BTFA while the apo sample was labeled with a stoichiometric concentration of BTFA to reduce labeling on the native cysteine in the ligand binding pocket. .. Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label.

    Ligand Binding Assay:

    Article Title: Definition of functionally and structurally distinct repressive states in the nuclear receptor PPARγ
    Article Snippet: However, the single mutant PPARγQ322C was labeled with a twofold molar excess of BTFA following drug addition with the following exceptions, samples loaded with the covalent ligands were labeled with a tenfold molar excess of BTFA while the apo sample was labeled with a stoichiometric concentration of BTFA to reduce labeling on the native cysteine in the ligand binding pocket. .. Following labeling samples were incubated for 30 min when loaded with ligand or 2 h for the apo sample and then buffer exchanged > 100× using an amicon ultra centrifugal filter (Millipore) with a 10 kDa molecular weight cut off to remove excess BTFA label.

    Staining:

    Article Title: Cell-Free Enzymatic Conversion of Spent Coffee Grounds Into the Platform Chemical Lactic Acid
    Article Snippet: The buffer of eluted enzyme solutions was exchanged to 50 mM HEPES pH 7.0 using an Amicon Ultra centrifugal filter units (4- and 30-kDa cutoff, Millipore). .. Purity and size of the enzymes were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by Coomassie staining.

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    Millipore amicon ultra centrifugal filters
    Amicon Ultra Centrifugal Filters, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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