E Coli Rnase H1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "A genetic selection reveals functional metastable structures embedded in a toxin-encoding mRNA"
Article Title: A genetic selection reveals functional metastable structures embedded in a toxin-encoding mRNA
Figure Legend Snippet: Gel analysis of RNase H/oligonucleotide accessibility assays. 30 fmol of internally labeled RNA were used. DNA oligonucleotides were used to a final concentration of 0 to 10 μM. Reactions were incubated for 30 min at 30°C in the presence or absence (C, control) of 0.25 U E. coli RNase H1. Reactions were stopped with 10 μl of 2X Loading Buffer and products were analyzed in an 8% denaturing PAA gel. See Figure 5C for relative band quantification. P1 and P2 indicate the two RNase H-oligonucleotide-mediated RNA cleavage products.
Techniques Used: Labeling, Concentration Assay, Incubation