Structured Review

Illumina Inc alu i digestion
Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated <t>Alu</t> I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to <t>Illumina</t> multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq
Alu I Digestion, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/alu i digestion/product/Illumina Inc
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
alu i digestion - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA"

Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA

Journal: BMC Biotechnology

doi: 10.1186/s12896-017-0409-7

Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated Alu I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to Illumina multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq
Figure Legend Snippet: Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated Alu I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to Illumina multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq

Techniques Used: Methylation, Amplification, Sequencing, Multiplexing, Polymerase Chain Reaction, Methylated DNA Immunoprecipitation

Related Articles

Sequencing:

Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA
Article Snippet: .. Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA). .. Illumina multiplexing adapter1 (sequence 5′-pho-GATCGGAAGAGCACACGTCT-3′) and adapter2 (sequence 5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′), in which all the Cs were un-methylated, were ligated to after A-tailing added the annealed DNA following the protocol of Illumina Solexa GAIIx.

Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA
Article Snippet: .. Library preparation and Illumina Solexa high-throughput sequencing Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA). .. Illumina multiplexing adapter1 (sequence 5′-pho-GATCGGAAGAGCACACGTCT-3′) and adapter2 (sequence 5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′), in which all the Cs were un-methylated, were ligated to after A-tailing added the annealed DNA following the protocol of Illumina Solexa GAIIx.

High Throughput Screening Assay:

Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA
Article Snippet: .. Library preparation and Illumina Solexa high-throughput sequencing Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA). .. Illumina multiplexing adapter1 (sequence 5′-pho-GATCGGAAGAGCACACGTCT-3′) and adapter2 (sequence 5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′), in which all the Cs were un-methylated, were ligated to after A-tailing added the annealed DNA following the protocol of Illumina Solexa GAIIx.

Construct:

Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA
Article Snippet: .. Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA). .. Illumina multiplexing adapter1 (sequence 5′-pho-GATCGGAAGAGCACACGTCT-3′) and adapter2 (sequence 5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′), in which all the Cs were un-methylated, were ligated to after A-tailing added the annealed DNA following the protocol of Illumina Solexa GAIIx.

Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA
Article Snippet: .. Library preparation and Illumina Solexa high-throughput sequencing Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA). .. Illumina multiplexing adapter1 (sequence 5′-pho-GATCGGAAGAGCACACGTCT-3′) and adapter2 (sequence 5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′), in which all the Cs were un-methylated, were ligated to after A-tailing added the annealed DNA following the protocol of Illumina Solexa GAIIx.

Purification:

Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA
Article Snippet: .. Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA). .. Illumina multiplexing adapter1 (sequence 5′-pho-GATCGGAAGAGCACACGTCT-3′) and adapter2 (sequence 5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′), in which all the Cs were un-methylated, were ligated to after A-tailing added the annealed DNA following the protocol of Illumina Solexa GAIIx.

Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA
Article Snippet: .. Library preparation and Illumina Solexa high-throughput sequencing Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA). .. Illumina multiplexing adapter1 (sequence 5′-pho-GATCGGAAGAGCACACGTCT-3′) and adapter2 (sequence 5′-ACACTCTTTCCCTACACGACGCTCTTCCGATCT-3′), in which all the Cs were un-methylated, were ligated to after A-tailing added the annealed DNA following the protocol of Illumina Solexa GAIIx.

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Illumina Inc alu i digestion
    Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated <t>Alu</t> I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to <t>Illumina</t> multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq
    Alu I Digestion, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alu i digestion/product/Illumina Inc
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    alu i digestion - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results


    Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated Alu I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to Illumina multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq

    Journal: BMC Biotechnology

    Article Title: DNA methylome profiling at single-base resolution through bisulfite sequencing of 5mC-immunoprecipitated DNA

    doi: 10.1186/s12896-017-0409-7

    Figure Lengend Snippet: Schematic outline of the MB-seq and MRB-seq experiment. a Schematic drawing of MB-seq approach. Genomic DNA was randomly fragmented to 100–300 bp and ligated to Alu I-linkers with a methylated Alu I recognition site close to the T-overhang. The ligated-fragments were captured using methylcytosine antibody, then treated with sodium bisulfite and converted to double stranded DNA by amplification using biotin labelled Alu I primers. The Alu I-linker was digested and removed by streptavidin coupled beads. The linker-removed sequence was added 3’end A-tailing, then ligated to Illumina multiplexing adaptors following PCR amplification using Illumina paired-end PCR primers. The PCR products of 230–250 bases in length were size-selected on a gel and sequenced on the Illumina platform. b Schematic drawing of MRB-seq approach. Repetitive DNA elements were removed using Cot-1 DNA after MeDIP (based on the MB-seq approach). Cot-1 DNA was labelled with biotin and coupled with streptavidin, and the streptavidin-biotin-Cot-1 DNA was hybridized to enriched methylated DNA fragments via MeDIP to remove repeat fragments. The methylated fragments obtained (single/low copy DNA fragments) were then subjected to sodium bisulfite treatment, PCR amplification, Alu I digestion and sequencing library preparation, as per MB-seq

    Article Snippet: Library preparation and Illumina Solexa high-throughput sequencing Sequencing libraries of purified DNA from Alu I digestion were constructed following the Illumina paired ends sequencing library protocol (Illumine, USA).

    Techniques: Methylation, Amplification, Sequencing, Multiplexing, Polymerase Chain Reaction, Methylated DNA Immunoprecipitation