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alpha-adaptin antibody  (NSJ Bioreagents)


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    NSJ Bioreagents alpha-adaptin antibody
    Alpha Adaptin Antibody, supplied by NSJ Bioreagents, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alpha-adaptin antibody/product/NSJ Bioreagents
    Average 94 stars, based on 1 article reviews
    alpha-adaptin antibody - by Bioz Stars, 2025-04
    94/100 stars

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    Mammary glands from lactating (5 days) mice of dab2 heterozygous controls and mosaic knockouts ( dab2 (f/df):Meox2-Cre) were analyzed by confocal immunofluorescence microscopy for several endocytosis and polarity markers. Where possible, markers were compared between Dab2-positive and Dab2-negative cells in the same section of the mosaic dab2 -deleted tissues. ( A ) Adaptin alpha and Dab2 double staining of mammary glands from a mosaic dab2 -deleted mouse, comparing Dab2-positive (arrow) and negative (arrowhead) cells; ( B ) Clathrin and Dab2 co-staining of mammary glands from a mosaic dab2 -deleted mouse, comparing Dab2-positive (arrow) and negative (arrowhead) cells; ( C ) Dab2 and E-cadherin staining: E-cadherin is localized more in the cytoplasm in Dab2-deleted cells (arrowhead); ( D ) NPT2b, a sodium-phosphate cotransporter; and ( E ) PMCA2 ATPase, a calcium pump, in Dab2-positive (arrow) and negative (arrowhead) cells. The results are the representation of multiple slides from 3 control (wildtype and heterozygous) and 3 dab2 conditional knockout mice.

    Journal: PLoS ONE

    Article Title: Hormonal Induction and Roles of Disabled-2 in Lactation and Involution

    doi: 10.1371/journal.pone.0110737

    Figure Lengend Snippet: Mammary glands from lactating (5 days) mice of dab2 heterozygous controls and mosaic knockouts ( dab2 (f/df):Meox2-Cre) were analyzed by confocal immunofluorescence microscopy for several endocytosis and polarity markers. Where possible, markers were compared between Dab2-positive and Dab2-negative cells in the same section of the mosaic dab2 -deleted tissues. ( A ) Adaptin alpha and Dab2 double staining of mammary glands from a mosaic dab2 -deleted mouse, comparing Dab2-positive (arrow) and negative (arrowhead) cells; ( B ) Clathrin and Dab2 co-staining of mammary glands from a mosaic dab2 -deleted mouse, comparing Dab2-positive (arrow) and negative (arrowhead) cells; ( C ) Dab2 and E-cadherin staining: E-cadherin is localized more in the cytoplasm in Dab2-deleted cells (arrowhead); ( D ) NPT2b, a sodium-phosphate cotransporter; and ( E ) PMCA2 ATPase, a calcium pump, in Dab2-positive (arrow) and negative (arrowhead) cells. The results are the representation of multiple slides from 3 control (wildtype and heterozygous) and 3 dab2 conditional knockout mice.

    Article Snippet: The sources and dilutions of antibodies are: anti-cytokeratin 8 (1/200) (Developmental Studies Hybridoma Bank, Ames, IA), anti-estrogen receptor alpha (1/500) (Santa Cruz, sc-543), anti-progesterone receptor (1/500) (Santa Cruz, sc-538), anti-adaptin-alpha (1/800) (BD Transduction Lab, 610501), anti-clathrin heavy chain (1/500) (BD Transduction Lab, 610499), anti-NPT2b (1/500) (Alpha Diagnostics Int.), and anti-PMCA2 (1/500) (ThermoFisher Scientific).

    Techniques: Immunofluorescence, Microscopy, Double Staining, Staining, Knock-Out