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(A) Dystrophin, nNOS and <t>alpha-Sarcoglycan</t> immunohistochemistry of TA in WT, d45-48, d45-47, d45-49 and mdx mice, at the age of 1 and 3 months. (B) Western blot analysis for dystrophin in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (C) Western blot analysis for dystrophin-homolog utrophin in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (D) Western blot analysis for nNOS in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (E) Relative Dp427 protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). (F) Relative Up395 protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). (G) Relative nNOS protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). Bar: mean ± S.D.; * P < 0.05, ** P < 0.01. Scale bar means 100 µm.
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(A) Dystrophin, nNOS and alpha-Sarcoglycan immunohistochemistry of TA in WT, d45-48, d45-47, d45-49 and mdx mice, at the age of 1 and 3 months. (B) Western blot analysis for dystrophin in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (C) Western blot analysis for dystrophin-homolog utrophin in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (D) Western blot analysis for nNOS in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (E) Relative Dp427 protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). (F) Relative Up395 protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). (G) Relative nNOS protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). Bar: mean ± S.D.; * P < 0.05, ** P < 0.01. Scale bar means 100 µm.

Journal: bioRxiv

Article Title: Becker muscular dystrophy mice showed site-specific decay of type IIa fibers with capillary change in skeletal muscle

doi: 10.1101/2024.10.15.618443

Figure Lengend Snippet: (A) Dystrophin, nNOS and alpha-Sarcoglycan immunohistochemistry of TA in WT, d45-48, d45-47, d45-49 and mdx mice, at the age of 1 and 3 months. (B) Western blot analysis for dystrophin in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (C) Western blot analysis for dystrophin-homolog utrophin in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (D) Western blot analysis for nNOS in WT, d45-48, d45-47, d45-49 and mdx mice, at the ages of 1 and 3 months. (E) Relative Dp427 protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). (F) Relative Up395 protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). (G) Relative nNOS protein levels (normalized by total protein band intensity) in WT, d45-48, d45-47, d45-49 and mdx mice (n=3). Bar: mean ± S.D.; * P < 0.05, ** P < 0.01. Scale bar means 100 µm.

Article Snippet: The primary antibodies were as follows: mouse anti-Dystrophin (NCL-DYS2, Leica Biosystems), rabbit anti-nNOS (61-7000, Invitrogen), mouse anti-alpha-Sarcoglycan (NCL-a-SARC, Leica Biosystems), mouse anti-MYH-1 (6H1, DSHB), mouse anti-MYH-2 (SC-71, DSHB), mouse anti-MYH-4 (BF-F3, DSHB), mouse anti-MYH-7 (BA-D5, Millipore), rat anti-PECAM-1 (550274, BD bioscience).

Techniques: Immunohistochemistry, Western Blot

Journal: Cell reports

Article Title: Thbs1 regulates skeletal muscle mass in a TGFβ-Smad2/3-ATF4-dependent manner

doi: 10.1016/j.celrep.2024.114149

Figure Lengend Snippet:

Article Snippet: Mouse monoclonal anti-alpha Sarcoglycan , Developmental Studies Hybridoma Bank (DSHB) , Cat# IVD3(1)A9; RRID:AB_2185657.

Techniques: Virus, Recombinant, Electron Microscopy, Plasmid Preparation, SYBR Green Assay, Membrane, Blocking Assay, Staining, Enzyme-linked Immunosorbent Assay, Activity Assay, Protein Extraction, Clone Assay, Expressing, Software, Microscopy, Real-time Polymerase Chain Reaction

Dilution factor for individual antibodies

Journal: Cureus

Article Title: Clinicopathological Profile of Muscle Diseases Presenting the Adult Population in Northern India: Preliminary Analysis in a Limited Resource Setting

doi: 10.7759/cureus.60084

Figure Lengend Snippet: Dilution factor for individual antibodies

Article Snippet: Alpha Sarcoglycan , Leica Novocastra liquid mouse monoclonal (adhalin) , Ad1/20A6 , IgG1 , 1:100.

Techniques:

Becker’s muscular dystrophy case: (A) H&E stain showing myophagocytosis (x40), (B) H&E stain showing regenerating fibers (x40), (C) dystrophin N IHC stain showing mild positivity (x20), (D) alpha sarcoglycan IHC stain showing positivity (x20), (E) gamma sarcoglycan IHC stain showing positivity (x20), (F) Spectrin IHC stain showing positivity (x20).

Journal: Cureus

Article Title: Clinicopathological Profile of Muscle Diseases Presenting the Adult Population in Northern India: Preliminary Analysis in a Limited Resource Setting

doi: 10.7759/cureus.60084

Figure Lengend Snippet: Becker’s muscular dystrophy case: (A) H&E stain showing myophagocytosis (x40), (B) H&E stain showing regenerating fibers (x40), (C) dystrophin N IHC stain showing mild positivity (x20), (D) alpha sarcoglycan IHC stain showing positivity (x20), (E) gamma sarcoglycan IHC stain showing positivity (x20), (F) Spectrin IHC stain showing positivity (x20).

Article Snippet: Alpha Sarcoglycan , Leica Novocastra liquid mouse monoclonal (adhalin) , Ad1/20A6 , IgG1 , 1:100.

Techniques: Staining

Dysferlinopathy case: (A) NADH staining showing Type I and Type II fibers (x20), (B) alpha sarcoglycan IHC stain showing positivity (x20), (C) dysferlin IHC stain showing negativity (x20), (D) dystrophin N IHC stain showing positivity (x20).

Journal: Cureus

Article Title: Clinicopathological Profile of Muscle Diseases Presenting the Adult Population in Northern India: Preliminary Analysis in a Limited Resource Setting

doi: 10.7759/cureus.60084

Figure Lengend Snippet: Dysferlinopathy case: (A) NADH staining showing Type I and Type II fibers (x20), (B) alpha sarcoglycan IHC stain showing positivity (x20), (C) dysferlin IHC stain showing negativity (x20), (D) dystrophin N IHC stain showing positivity (x20).

Article Snippet: Alpha Sarcoglycan , Leica Novocastra liquid mouse monoclonal (adhalin) , Ad1/20A6 , IgG1 , 1:100.

Techniques: Staining

Alpha sarcoglycanopathy case: (A) H&E stain showing variation in muscle fiber (x10), (B) Alpha sarcoglycan IHC stain showing negativity (x20), (C) beta sarcoglycan IHC stain showing positivity (x20), (D) Gamma sarcoglycan IHC stain showing positivity (x20).

Journal: Cureus

Article Title: Clinicopathological Profile of Muscle Diseases Presenting the Adult Population in Northern India: Preliminary Analysis in a Limited Resource Setting

doi: 10.7759/cureus.60084

Figure Lengend Snippet: Alpha sarcoglycanopathy case: (A) H&E stain showing variation in muscle fiber (x10), (B) Alpha sarcoglycan IHC stain showing negativity (x20), (C) beta sarcoglycan IHC stain showing positivity (x20), (D) Gamma sarcoglycan IHC stain showing positivity (x20).

Article Snippet: Alpha Sarcoglycan , Leica Novocastra liquid mouse monoclonal (adhalin) , Ad1/20A6 , IgG1 , 1:100.

Techniques: Staining