allprep dna rna mirna universal kit protocol  (Qiagen)

 
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    Name:
    AllPrep DNA RNA miRNA Universal Kit
    Description:
    For simultaneous purification of genomic DNA and total RNA including miRNA from cells and difficult to lyse tissue Kit contents Qiagen AllPrep DNA RNA miRNA Universal Kit 50 preps Developed for Difficult to lyse Samples High Yields of DNA RNA and miRNA from the Same Sample Delivers High quality Nucleic Acids Ready for Downstream use For Simultaneous Purification of Genomic DNA and Total RNA Including miRNA from Cells and Difficult to lyse Tissue Includes 50 AllPrep DNA Mini Spin Columns 50 RNeasy Mini Spin Columns Collection Tubes and Buffers Benefits High yields of DNA RNA and miRNA from the same sample Developed for difficult to lyse samples e g fiber or lipid rich tissue Delivers high quality nucleic acids ready for downstream use Preoptimized protocols for various sample types Convenient procedure that does not require use of pheno
    Catalog Number:
    80224
    Price:
    641
    Category:
    AllPrep DNA RNA miRNA Universal Kit
    Buy from Supplier


    Structured Review

    Qiagen allprep dna rna mirna universal kit protocol
    AllPrep DNA RNA miRNA Universal Kit
    For simultaneous purification of genomic DNA and total RNA including miRNA from cells and difficult to lyse tissue Kit contents Qiagen AllPrep DNA RNA miRNA Universal Kit 50 preps Developed for Difficult to lyse Samples High Yields of DNA RNA and miRNA from the Same Sample Delivers High quality Nucleic Acids Ready for Downstream use For Simultaneous Purification of Genomic DNA and Total RNA Including miRNA from Cells and Difficult to lyse Tissue Includes 50 AllPrep DNA Mini Spin Columns 50 RNeasy Mini Spin Columns Collection Tubes and Buffers Benefits High yields of DNA RNA and miRNA from the same sample Developed for difficult to lyse samples e g fiber or lipid rich tissue Delivers high quality nucleic acids ready for downstream use Preoptimized protocols for various sample types Convenient procedure that does not require use of pheno
    https://www.bioz.com/result/allprep dna rna mirna universal kit protocol/product/Qiagen
    Average 99 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    allprep dna rna mirna universal kit protocol - by Bioz Stars, 2020-08
    99/100 stars

    Related Products / Commonly Used Together

    rna-free dna
    combined extraction
    tissuelyser
    dna-free rna

    Images

    1) Product Images from "RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition"

    Article Title: RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0121659

    Comparison of DNA/RNA yields and RNA quality (inset). (A) Comparison between the AllPrep standard protocol (APS; described below figure), enzymatic lysis (Enz), enzymatic/bead beating (Bead), and Mirvana extraction protocols (Mirvana). (B) Comparison between different iterations of the AP protocol, examining the effect of preservation method {P}, lysis conditions {L}, and other extraction modification steps {E}. The data presents changes in yield relative to a control, where each column shows the effect of one factor relative to a control that differed in that factor only. The control was extracted using either the AP standard protocol or a modification as indicated by the X-axis labels below the horizontal lines. The factor that was changed is indicated just below the data column. The dotted line indicates no change relative to control. All data represent averages of three extractions, with error bars indicating the 95% confidence interval, except for the effect of lysozyme, which averaged the relative yield effects from multiple samples (from DL, LH, and HR samples, three replicate extractions each). Inset: RIN = RNA integrity number quality (0 (poor)- 10 (high) range) assessment by Bioanalyzer; NT = no preservation treatment.
    Figure Legend Snippet: Comparison of DNA/RNA yields and RNA quality (inset). (A) Comparison between the AllPrep standard protocol (APS; described below figure), enzymatic lysis (Enz), enzymatic/bead beating (Bead), and Mirvana extraction protocols (Mirvana). (B) Comparison between different iterations of the AP protocol, examining the effect of preservation method {P}, lysis conditions {L}, and other extraction modification steps {E}. The data presents changes in yield relative to a control, where each column shows the effect of one factor relative to a control that differed in that factor only. The control was extracted using either the AP standard protocol or a modification as indicated by the X-axis labels below the horizontal lines. The factor that was changed is indicated just below the data column. The dotted line indicates no change relative to control. All data represent averages of three extractions, with error bars indicating the 95% confidence interval, except for the effect of lysozyme, which averaged the relative yield effects from multiple samples (from DL, LH, and HR samples, three replicate extractions each). Inset: RIN = RNA integrity number quality (0 (poor)- 10 (high) range) assessment by Bioanalyzer; NT = no preservation treatment.

    Techniques Used: Lysis, Preserving, Modification

    Related Articles

    Modification:

    Article Title: Estradiol Treatment Initiated Early After Ovariectomy Regulates Myocardial Gene Expression and Inhibits Diastolic Dysfunction in Female Cynomolgus Monkeys: Potential Roles for Calcium Homeostasis and Extracellular Matrix Remodeling
    Article Snippet: .. Myocardial RNA Isolation and Microarray Analysis of Gene Expression RNA was isolated from frozen tissue sections (10–30 mg) using AllPrep ® DNA/RNA/miRNA kits (Qiagen, Valencia, CA) according to a modified manufacturer protocol using 1% β‐mercaptoethanol. ..

    Isolation:

    Article Title: Integrative microRNA and mRNA deep-sequencing expression profiling in endemic Burkitt lymphoma
    Article Snippet: .. RNA and small RNA isolation Total RNA and Small RNA molecules were extracted from eBL FNA samples in RNAlater using the AllPrep DNA/RNA/miRNA Universal kit (Qiagen) according to manufacturer’s instructions. .. Small RNA abundance and integrity were determined after isolation using Nanodrop-ND-1000 spectrophotometer (Thermo Fisher Scientific, Waltham, Massachusetts, USA) and an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA), respectively.

    Article Title: Estradiol Treatment Initiated Early After Ovariectomy Regulates Myocardial Gene Expression and Inhibits Diastolic Dysfunction in Female Cynomolgus Monkeys: Potential Roles for Calcium Homeostasis and Extracellular Matrix Remodeling
    Article Snippet: .. Myocardial RNA Isolation and Microarray Analysis of Gene Expression RNA was isolated from frozen tissue sections (10–30 mg) using AllPrep ® DNA/RNA/miRNA kits (Qiagen, Valencia, CA) according to a modified manufacturer protocol using 1% β‐mercaptoethanol. ..

    other:

    Article Title: Transcriptome-wide analysis of natural antisense transcripts shows their potential role in breast cancer
    Article Snippet: DNA/RNA/miRNA extraction DNA, RNA and miRNA were simultaneously extracted using an All Prep DNA/RNA/miRNA Universal kit (Qiagen, Belgium) according to the manufacturer’s protocol.

    Expressing:

    Article Title: Estradiol Treatment Initiated Early After Ovariectomy Regulates Myocardial Gene Expression and Inhibits Diastolic Dysfunction in Female Cynomolgus Monkeys: Potential Roles for Calcium Homeostasis and Extracellular Matrix Remodeling
    Article Snippet: .. Myocardial RNA Isolation and Microarray Analysis of Gene Expression RNA was isolated from frozen tissue sections (10–30 mg) using AllPrep ® DNA/RNA/miRNA kits (Qiagen, Valencia, CA) according to a modified manufacturer protocol using 1% β‐mercaptoethanol. ..

    Microarray:

    Article Title: Estradiol Treatment Initiated Early After Ovariectomy Regulates Myocardial Gene Expression and Inhibits Diastolic Dysfunction in Female Cynomolgus Monkeys: Potential Roles for Calcium Homeostasis and Extracellular Matrix Remodeling
    Article Snippet: .. Myocardial RNA Isolation and Microarray Analysis of Gene Expression RNA was isolated from frozen tissue sections (10–30 mg) using AllPrep ® DNA/RNA/miRNA kits (Qiagen, Valencia, CA) according to a modified manufacturer protocol using 1% β‐mercaptoethanol. ..

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  • 99
    Qiagen allprep dna rna mirna universal kit protocol
    Comparison of <t>DNA/RNA</t> yields and RNA quality (inset). (A) Comparison between the <t>AllPrep</t> standard protocol (APS; described below figure), enzymatic lysis (Enz), enzymatic/bead beating (Bead), and Mirvana extraction protocols (Mirvana). (B) Comparison between different iterations of the AP protocol, examining the effect of preservation method {P}, lysis conditions {L}, and other extraction modification steps {E}. The data presents changes in yield relative to a control, where each column shows the effect of one factor relative to a control that differed in that factor only. The control was extracted using either the AP standard protocol or a modification as indicated by the X-axis labels below the horizontal lines. The factor that was changed is indicated just below the data column. The dotted line indicates no change relative to control. All data represent averages of three extractions, with error bars indicating the 95% confidence interval, except for the effect of lysozyme, which averaged the relative yield effects from multiple samples (from DL, LH, and HR samples, three replicate extractions each). Inset: RIN = RNA integrity number quality (0 (poor)- 10 (high) range) assessment by Bioanalyzer; NT = no preservation treatment.
    Allprep Dna Rna Mirna Universal Kit Protocol, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/allprep dna rna mirna universal kit protocol/product/Qiagen
    Average 99 stars, based on 17 article reviews
    Price from $9.99 to $1999.99
    allprep dna rna mirna universal kit protocol - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    Image Search Results


    Comparison of DNA/RNA yields and RNA quality (inset). (A) Comparison between the AllPrep standard protocol (APS; described below figure), enzymatic lysis (Enz), enzymatic/bead beating (Bead), and Mirvana extraction protocols (Mirvana). (B) Comparison between different iterations of the AP protocol, examining the effect of preservation method {P}, lysis conditions {L}, and other extraction modification steps {E}. The data presents changes in yield relative to a control, where each column shows the effect of one factor relative to a control that differed in that factor only. The control was extracted using either the AP standard protocol or a modification as indicated by the X-axis labels below the horizontal lines. The factor that was changed is indicated just below the data column. The dotted line indicates no change relative to control. All data represent averages of three extractions, with error bars indicating the 95% confidence interval, except for the effect of lysozyme, which averaged the relative yield effects from multiple samples (from DL, LH, and HR samples, three replicate extractions each). Inset: RIN = RNA integrity number quality (0 (poor)- 10 (high) range) assessment by Bioanalyzer; NT = no preservation treatment.

    Journal: PLoS ONE

    Article Title: RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition

    doi: 10.1371/journal.pone.0121659

    Figure Lengend Snippet: Comparison of DNA/RNA yields and RNA quality (inset). (A) Comparison between the AllPrep standard protocol (APS; described below figure), enzymatic lysis (Enz), enzymatic/bead beating (Bead), and Mirvana extraction protocols (Mirvana). (B) Comparison between different iterations of the AP protocol, examining the effect of preservation method {P}, lysis conditions {L}, and other extraction modification steps {E}. The data presents changes in yield relative to a control, where each column shows the effect of one factor relative to a control that differed in that factor only. The control was extracted using either the AP standard protocol or a modification as indicated by the X-axis labels below the horizontal lines. The factor that was changed is indicated just below the data column. The dotted line indicates no change relative to control. All data represent averages of three extractions, with error bars indicating the 95% confidence interval, except for the effect of lysozyme, which averaged the relative yield effects from multiple samples (from DL, LH, and HR samples, three replicate extractions each). Inset: RIN = RNA integrity number quality (0 (poor)- 10 (high) range) assessment by Bioanalyzer; NT = no preservation treatment.

    Article Snippet: The combined extraction method was based on the AllPrep DNA/RNA/miRNA Universal kit protocol (Qiagen).

    Techniques: Lysis, Preserving, Modification