allprep dna rna micro kit  (Qiagen)


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    AllPrep DNA RNA Micro Kit
    Description:
    For simultaneous purification of DNA and RNA from small cell and tissue samples Kit contents Qiagen AllPrep DNA RNA Micro Kit 50 preps 5mg Tissue Sample DNA 30L RNA 10L Elution Volume Cells Tissue Sample Silica Technology Manual Processing DNA and RNA Purification 35 min Time Run Ideal for PCR RT PCR cDNA Synthesis Microarray Blotting For Simultaneous Purification of DNA and RNA from Small Cell and Tissue Samples Includes AllPrep DNA Spin Columns RNeasy minElute Spin Columns Collection Tubes Carrier RNA RNase free Water and Buffers Benefits High quality DNA and RNA from the same sample Maximal yields of DNA and RNA from precious samples Rapid purification with short streamlined protocol Ready to use DNA and RNA for any downstream analysis
    Catalog Number:
    80284
    Price:
    722
    Category:
    AllPrep DNA RNA Micro Kit
    Buy from Supplier


    Structured Review

    Qiagen allprep dna rna micro kit
    AllPrep DNA RNA Micro Kit
    For simultaneous purification of DNA and RNA from small cell and tissue samples Kit contents Qiagen AllPrep DNA RNA Micro Kit 50 preps 5mg Tissue Sample DNA 30L RNA 10L Elution Volume Cells Tissue Sample Silica Technology Manual Processing DNA and RNA Purification 35 min Time Run Ideal for PCR RT PCR cDNA Synthesis Microarray Blotting For Simultaneous Purification of DNA and RNA from Small Cell and Tissue Samples Includes AllPrep DNA Spin Columns RNeasy minElute Spin Columns Collection Tubes Carrier RNA RNase free Water and Buffers Benefits High quality DNA and RNA from the same sample Maximal yields of DNA and RNA from precious samples Rapid purification with short streamlined protocol Ready to use DNA and RNA for any downstream analysis
    https://www.bioz.com/result/allprep dna rna micro kit/product/Qiagen
    Average 90 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    allprep dna rna micro kit - by Bioz Stars, 2020-01
    90/100 stars

    Images

    1) Product Images from "Bombesin-like receptor 3 (Brs3) expression in glutamatergic, but not GABAergic, neurons is required for regulation of energy metabolism"

    Article Title: Bombesin-like receptor 3 (Brs3) expression in glutamatergic, but not GABAergic, neurons is required for regulation of energy metabolism

    Journal: Molecular Metabolism

    doi: 10.1016/j.molmet.2017.08.013

    Generation of Brs3 fl/y mice. (A) Scheme for creation of floxed Brs3 allele. Homologous recombination of the Brs3 targeting construct in embryonic stem (ES) cells produced the targeted allele, and a founder was bred with an EIIa-Cre female to remove the PGK-Neo cassette. Deletion of exon 2 by Cre recombinase generates the null allele. (B) Southern hybridization of ES DNA digested with EcoRV using a 5′ flanking probe, detected bands of 5.4 kb (WT) and 3.8 kb (floxed). (C) PCR genotyping using primers x213 and x214, produces products of 203 bp (WT) and 237 bp (floxed). (D, E) Brs3 DNA and RNA were quantified by PCR in hypothalamus of (D) Brs3 fl/y ;Vglut2-Cre (Vglut2-Cre) or (E) Brs3 fl/y ;Vgat-Cre (Vgat-Cre) and littermate control Brs3 fl/y (flox/y) mice. Primers x213 and x214 were used for DNA and x573 and x574 for RNA. N = 4–5/group; * indicates P
    Figure Legend Snippet: Generation of Brs3 fl/y mice. (A) Scheme for creation of floxed Brs3 allele. Homologous recombination of the Brs3 targeting construct in embryonic stem (ES) cells produced the targeted allele, and a founder was bred with an EIIa-Cre female to remove the PGK-Neo cassette. Deletion of exon 2 by Cre recombinase generates the null allele. (B) Southern hybridization of ES DNA digested with EcoRV using a 5′ flanking probe, detected bands of 5.4 kb (WT) and 3.8 kb (floxed). (C) PCR genotyping using primers x213 and x214, produces products of 203 bp (WT) and 237 bp (floxed). (D, E) Brs3 DNA and RNA were quantified by PCR in hypothalamus of (D) Brs3 fl/y ;Vglut2-Cre (Vglut2-Cre) or (E) Brs3 fl/y ;Vgat-Cre (Vgat-Cre) and littermate control Brs3 fl/y (flox/y) mice. Primers x213 and x214 were used for DNA and x573 and x574 for RNA. N = 4–5/group; * indicates P

    Techniques Used: Mouse Assay, Homologous Recombination, Construct, Produced, Hybridization, Polymerase Chain Reaction

    Generation of Brs3 loxTB/y mice. (A) A loxP-flanked transcriptional blocker (loxTB) was inserted in the first intron of Brs3 . Cre recombinase will cause re-expression of Brs3 from the silent Brs3 loxTB allele. (B) PCR (primers x559, x561, and x562) genotyping gives 214 bp wild type (WT) and 395 bp unrecombined loxTB products. (C) The fraction of recombined loxTB DNA was measured by subtracting the unrecombined level in the Cre-expressing mice from that in loxTB mice. PCR (primers x669 and x212) was performed on hypothalamus DNA from Brs3 loxTB/y (loxTB), Brs3 loxTB/y ;Vglut2-Cre (Vglut2-Cre), and Brs3 loxTB/y ;Vgat-Cre (Vgat-Cre) mice. (D) Brs3 RNA in hypothalamus was quantified by RT-PCR (using primers x573 and x574). N = 4–5/group.
    Figure Legend Snippet: Generation of Brs3 loxTB/y mice. (A) A loxP-flanked transcriptional blocker (loxTB) was inserted in the first intron of Brs3 . Cre recombinase will cause re-expression of Brs3 from the silent Brs3 loxTB allele. (B) PCR (primers x559, x561, and x562) genotyping gives 214 bp wild type (WT) and 395 bp unrecombined loxTB products. (C) The fraction of recombined loxTB DNA was measured by subtracting the unrecombined level in the Cre-expressing mice from that in loxTB mice. PCR (primers x669 and x212) was performed on hypothalamus DNA from Brs3 loxTB/y (loxTB), Brs3 loxTB/y ;Vglut2-Cre (Vglut2-Cre), and Brs3 loxTB/y ;Vgat-Cre (Vgat-Cre) mice. (D) Brs3 RNA in hypothalamus was quantified by RT-PCR (using primers x573 and x574). N = 4–5/group.

    Techniques Used: Mouse Assay, Expressing, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction

    2) Product Images from "Intraindividual dynamics of transcriptome and genome-wide stability of DNA methylation"

    Article Title: Intraindividual dynamics of transcriptome and genome-wide stability of DNA methylation

    Journal: Scientific Reports

    doi: 10.1038/srep26424

    Sample summaries. ( a ) Monocyte gating strategy. Typical light-scatter density-plot of PBMCs (left) and CD14 high CD16 low monocytes were isolated from the monocyte-containing gate based on CD14 and CD16 expression (right). ( b ) Isolated CD14 high CD16 low monocyte population (96.7% ± 1.0% purity). ( c ) Cell numbers (left) and DNA (middle) and RNA (right) yields obtained for each cell population. ( d ) Estimated cellular composition for each sample as determined by reference information on cell-specific DNA methylation signatures using the “estimateCellCounts” function implemented in the minfi package. Bars in ( c , d ) represent mean ± standard deviation (grey, participant #1; black, participant #2).
    Figure Legend Snippet: Sample summaries. ( a ) Monocyte gating strategy. Typical light-scatter density-plot of PBMCs (left) and CD14 high CD16 low monocytes were isolated from the monocyte-containing gate based on CD14 and CD16 expression (right). ( b ) Isolated CD14 high CD16 low monocyte population (96.7% ± 1.0% purity). ( c ) Cell numbers (left) and DNA (middle) and RNA (right) yields obtained for each cell population. ( d ) Estimated cellular composition for each sample as determined by reference information on cell-specific DNA methylation signatures using the “estimateCellCounts” function implemented in the minfi package. Bars in ( c , d ) represent mean ± standard deviation (grey, participant #1; black, participant #2).

    Techniques Used: Isolation, Expressing, DNA Methylation Assay, Standard Deviation

    Related Articles

    Methylation Sequencing:

    Article Title: Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
    Article Snippet: Methylation Sequencing Genomic DNA was isolated and purified from skeletal muscles and thyroid glands of 90-day-old chickens using an AllPrep DNA/RNA Micro Kit (QIAGEN). .. Sodium bisulfite conversion bisulfite-sequencing PCR (BSP) was performed using the EpiTech Bisulfite Kit (QIAGEN).

    Clone Assay:

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
    Article Snippet: For comparative purposes we selected two clones (C5 and C6) containing an empty vector (pIRES2-EGFP(-)) and three clones (F5, F7 and F9) with full vector (pIRES2-EGFP-GalNAc4S-6ST). .. To establish the incorporation of the pIRES2-EGFP-GalNAc4S-6ST into genomic DNA, total genomic DNA was isolated from SKOV3 cells using a Qiagen AllPrep DNA/RNA micro kit (Qiagen GmbH, Hilden, Germany) according to manufacturer's specifications.

    Amplification:

    Article Title: Cervicovaginal bacteria are a major modulator of host inflammatory responses in the female genital tract
    Article Snippet: Shotgun DNA libraries were made from total nucleic acid isolated from 12 cervical swab samples; the same total nucleic acid was used for 16S amplification. .. DNA was isolated from the total nucleic acid using an AllPrep DNA/RNA Micro Kit (Qiagen) and sheared (S2 sonicator, Covaris).

    Article Title: Imaging genetics of FOXP2 in dyslexia
    Article Snippet: Total DNA and RNA were isolated using the AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany). .. A volume of 50 ng of total RNA were amplified (Illumina TotalPrep 96-RNA Amplification Kit, Ambion/Applied Biosystems, Darmstadt, Germany).

    Article Title: Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens
    Article Snippet: DNA was extracted from a 350 µl aliquot of transport medium with the AllPrep DNA/RNA Micro Kit (Qiagen GmbH, Hilden, Germany). β-actin DNA quantification was tested by real-time PCR with QuantiTect SYBRGreen PCR (Qiagen GmbH, Hilden, Germany) using the primers 5′ CCA ACC GCG AGA AGA TGA CC 3′ (forward) and 5′ GAT CTT CAT GAG GTA GTC AGT 3′ (reverse), corresponding to positions (5′) 382–617 (3′) of the human β-actin gene (Eurofins mwg Operon, Ebersberg, Germany). .. This resulted in amplification of a 236 bp fragment, possessing a molecular weight of 15.8 kD based on an assumed average molecular weight of 660 Dalton per basepair.

    Stable Transfection:

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
    Article Snippet: Paragraph title: Generation of stably transfected SKOV-3 cell lines with DNA encoding for GalNAc4S-6ST ... To establish the incorporation of the pIRES2-EGFP-GalNAc4S-6ST into genomic DNA, total genomic DNA was isolated from SKOV3 cells using a Qiagen AllPrep DNA/RNA micro kit (Qiagen GmbH, Hilden, Germany) according to manufacturer's specifications.

    Quantitative RT-PCR:

    Article Title: Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples
    Article Snippet: .. Samples for 16S rRNA RT qPCR were subjected to combined DNA/RNA extraction by means of the AllPrep DNA/RNA Micro Kit (Qiagen) at INH (Additional file : Protocol 5 & 7). .. Subsequently, whole transcriptome RNA was transcribed to complementary DNA by means of the QuantiTect Reverse Transcription Kit (Qiagen) with random hexamer primers, genomic DNA digestion and a gDNA wipe-out control (Additional file : Protocol 7) and samples were transported to DITM.

    Real-time Polymerase Chain Reaction:

    Article Title: Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples
    Article Snippet: Samples for RLEP qPCR were stored at − 20 °C at INH until shipment to DITM by courier service and subsequently extracted by means of the Gentra Puregene method (Qiagen, Additional file : Protocol 1) for samples in CLS, or by means of a FastPrep extraction (Bio-Budget, Additional file : Protocol 6) for samples in TLS. .. Samples for 16S rRNA RT qPCR were subjected to combined DNA/RNA extraction by means of the AllPrep DNA/RNA Micro Kit (Qiagen) at INH (Additional file : Protocol 5 & 7).

    Article Title: Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens
    Article Snippet: .. DNA was extracted from a 350 µl aliquot of transport medium with the AllPrep DNA/RNA Micro Kit (Qiagen GmbH, Hilden, Germany). β-actin DNA quantification was tested by real-time PCR with QuantiTect SYBRGreen PCR (Qiagen GmbH, Hilden, Germany) using the primers 5′ CCA ACC GCG AGA AGA TGA CC 3′ (forward) and 5′ GAT CTT CAT GAG GTA GTC AGT 3′ (reverse), corresponding to positions (5′) 382–617 (3′) of the human β-actin gene (Eurofins mwg Operon, Ebersberg, Germany). .. This resulted in amplification of a 236 bp fragment, possessing a molecular weight of 15.8 kD based on an assumed average molecular weight of 660 Dalton per basepair.

    Article Title: Prognostic Impact of HER2 and ER Status of Circulating Tumor Cells in Metastatic Breast Cancer Patients with a HER2-Negative Primary Tumor
    Article Snippet: Larger than 200 bp RNA was isolated using the AllPrep DNA/RNA Micro Kit (Qiagen, Germantown, MD). .. Generation of cDNA, preamplification, and reverse transcription quantitative polymerase chain reaction to quantify the expression of ESR1 were performed as described in detail before using a validated ESR1 Taqman assay (Hs00174860 m1; Applied Biosystems, San Francisco, CA) .

    Random Hexamer Labeling:

    Article Title: Development of a combined RLEP/16S rRNA (RT) qPCR assay for the detection of viable M. leprae from nasal swab samples
    Article Snippet: Samples for 16S rRNA RT qPCR were subjected to combined DNA/RNA extraction by means of the AllPrep DNA/RNA Micro Kit (Qiagen) at INH (Additional file : Protocol 5 & 7). .. Subsequently, whole transcriptome RNA was transcribed to complementary DNA by means of the QuantiTect Reverse Transcription Kit (Qiagen) with random hexamer primers, genomic DNA digestion and a gDNA wipe-out control (Additional file : Protocol 7) and samples were transported to DITM.

    Expressing:

    Article Title: Translational study identifies XPF and MUS81 as predictive biomarkers for oxaliplatin-based peri-operative chemotherapy in patients with esophageal adenocarcinoma
    Article Snippet: Gene expression was measured in the tumor biopsies and post-surgical resection samples. .. RNA was extracted using the Qiagen AllPrep DNA/RNA Micro Kit (Qiagen Inc., Valencia, CA) following the manufacturer’s instructions.

    Article Title: Imaging genetics of FOXP2 in dyslexia
    Article Snippet: Paragraph title: Analysis of differential allelic gene expression in human hippocampus ... Total DNA and RNA were isolated using the AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany).

    Article Title: Sonic Hedgehog Ligand: A Role in Formation of a Mesenchymal Niche in Human Pancreatic Ductal Adenocarcinoma
    Article Snippet: .. RNA Extraction, cDNA Synthesis, and Gene Expression Analysis Total RNA was extracted from cells using TRI reagent (Sigma-Aldrich), and from tissue samples using the AllPrep DNA/RNA Micro kit (Qiagen, Crawley, UK). .. RNA samples were reverse transcribed into cDNA using Superscript II Reverse Transcriptase (ThermoFisher Scientific).

    Article Title: Prognostic Impact of HER2 and ER Status of Circulating Tumor Cells in Metastatic Breast Cancer Patients with a HER2-Negative Primary Tumor
    Article Snippet: Larger than 200 bp RNA was isolated using the AllPrep DNA/RNA Micro Kit (Qiagen, Germantown, MD). .. Generation of cDNA, preamplification, and reverse transcription quantitative polymerase chain reaction to quantify the expression of ESR1 were performed as described in detail before using a validated ESR1 Taqman assay (Hs00174860 m1; Applied Biosystems, San Francisco, CA) .

    Transformation Assay:

    Article Title: Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
    Article Snippet: Methylation Sequencing Genomic DNA was isolated and purified from skeletal muscles and thyroid glands of 90-day-old chickens using an AllPrep DNA/RNA Micro Kit (QIAGEN). .. BSP products were purified, ligated, and transformed using the pGEM-T Easy Vector system I (Promega, Madison, WI, USA).

    Transfection:

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
    Article Snippet: Paragraph title: Generation of stably transfected SKOV-3 cell lines with DNA encoding for GalNAc4S-6ST ... To establish the incorporation of the pIRES2-EGFP-GalNAc4S-6ST into genomic DNA, total genomic DNA was isolated from SKOV3 cells using a Qiagen AllPrep DNA/RNA micro kit (Qiagen GmbH, Hilden, Germany) according to manufacturer's specifications.

    Ligation:

    Article Title: Genome-wide DNA methylation patterns of bovine blastocysts derived from in vivo embryos subjected to in vitro culture before, during or after embryonic genome activation
    Article Snippet: Paragraph title: DNA fragmentation, adapter ligation and methyl sensitive enzymes digestion ... Genomic DNA (gDNA) from four replicates (10 blastocysts per replicate) of the 2C_Flush, 8C_Flush, 16C_Flush and vivo blastocyst groups was isolated using the Allprep DNA/RNA micro kit (Qiagen, Germany) following the manufacturer’s recommendations.

    DNA Sequencing:

    Article Title: Cervicovaginal bacteria are a major modulator of host inflammatory responses in the female genital tract
    Article Snippet: Paragraph title: Metagenomic shotgun DNA sequencing and taxonomic classification ... DNA was isolated from the total nucleic acid using an AllPrep DNA/RNA Micro Kit (Qiagen) and sheared (S2 sonicator, Covaris).

    Polymerase Chain Reaction:

    Article Title: Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
    Article Snippet: Methylation Sequencing Genomic DNA was isolated and purified from skeletal muscles and thyroid glands of 90-day-old chickens using an AllPrep DNA/RNA Micro Kit (QIAGEN). .. Sodium bisulfite conversion bisulfite-sequencing PCR (BSP) was performed using the EpiTech Bisulfite Kit (QIAGEN).

    Article Title: Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens
    Article Snippet: .. DNA was extracted from a 350 µl aliquot of transport medium with the AllPrep DNA/RNA Micro Kit (Qiagen GmbH, Hilden, Germany). β-actin DNA quantification was tested by real-time PCR with QuantiTect SYBRGreen PCR (Qiagen GmbH, Hilden, Germany) using the primers 5′ CCA ACC GCG AGA AGA TGA CC 3′ (forward) and 5′ GAT CTT CAT GAG GTA GTC AGT 3′ (reverse), corresponding to positions (5′) 382–617 (3′) of the human β-actin gene (Eurofins mwg Operon, Ebersberg, Germany). .. This resulted in amplification of a 236 bp fragment, possessing a molecular weight of 15.8 kD based on an assumed average molecular weight of 660 Dalton per basepair.

    Software:

    Article Title: Translational study identifies XPF and MUS81 as predictive biomarkers for oxaliplatin-based peri-operative chemotherapy in patients with esophageal adenocarcinoma
    Article Snippet: RNA was extracted using the Qiagen AllPrep DNA/RNA Micro Kit (Qiagen Inc., Valencia, CA) following the manufacturer’s instructions. .. Raw gene expression data was processed using Illumina GenomeStudio software (v1.6) to generate expression intensities which were considered in a logged base 2 scale.

    Molecular Weight:

    Article Title: Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens
    Article Snippet: DNA was extracted from a 350 µl aliquot of transport medium with the AllPrep DNA/RNA Micro Kit (Qiagen GmbH, Hilden, Germany). β-actin DNA quantification was tested by real-time PCR with QuantiTect SYBRGreen PCR (Qiagen GmbH, Hilden, Germany) using the primers 5′ CCA ACC GCG AGA AGA TGA CC 3′ (forward) and 5′ GAT CTT CAT GAG GTA GTC AGT 3′ (reverse), corresponding to positions (5′) 382–617 (3′) of the human β-actin gene (Eurofins mwg Operon, Ebersberg, Germany). .. This resulted in amplification of a 236 bp fragment, possessing a molecular weight of 15.8 kD based on an assumed average molecular weight of 660 Dalton per basepair.

    DNA Extraction:

    Article Title: Chemical and Metagenomic Studies of the Lethal Black Band Disease of Corals Reveal Two Broadly Distributed, Redox-Sensitive Mixed Polyketide/Peptide Macrocycles
    Article Snippet: .. Identifying the Biosynthetic Gene Cluster DNA was extracted from the BBD layer or from the unicyanobacterial culture with a PowerSoil DNA isolation kit (MoBio) or Qiagen AllPrep DNA/RNA Micro kit, as previously described. .. Metagenomic libraries were constructed with a TruSeq DNA sample preparation kit (Illumina, San Diego, CA, USA) and sequenced at the University of Maryland Institute for Bioscience and Biotechnology Research on an Illumina HiSeq with a 100 bp paired-end protocol.

    RNA Sequencing Assay:

    Article Title: Cadmium Exposure Inhibits Branching Morphogenesis and Causes Alterations Consistent With HIF-1α Inhibition in Human Primary Breast Organoids
    Article Snippet: Paragraph title: RNA sequencing of primary mammospheres ... After 7 days of growth in primary mammosphere formation conditions, we extracted RNA from the control or cadmium treated mammospheres using the AllPrep DNA/RNA micro kit (Qiagen), including an on-column DNase treatment for RNA extraction.

    Article Title: Maternal Experience with Predation Risk Influences Genome-Wide Embryonic Gene Expression in Threespined Sticklebacks (Gasterosteus aculeatus)
    Article Snippet: Paragraph title: RNA Sequencing ... Embryo pools were homogenized and total RNA extracted from the homogenate using the AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (as in ).

    Methylation:

    Article Title: Intragenic sequences in the trophectoderm harbour the greatest proportion of methylation errors in day 17 bovine conceptuses generated using assisted reproductive technologies
    Article Snippet: Day 17 conceptuses were processed for methylation array analysis by dissecting three embryonic regions from all control (AI) and treatment group samples (SOV and IVP) immediately after recovery from the reproductive tract. .. Genomic DNA and total RNA were isolated from single, dissected day 17 conceptuses using the AllPrep DNA/RNA Micro Kit (Qiagen, Manchester, UK) according to the manufacturers’ guidelines.

    Article Title: Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
    Article Snippet: .. Methylation Sequencing Genomic DNA was isolated and purified from skeletal muscles and thyroid glands of 90-day-old chickens using an AllPrep DNA/RNA Micro Kit (QIAGEN). .. Sodium bisulfite conversion bisulfite-sequencing PCR (BSP) was performed using the EpiTech Bisulfite Kit (QIAGEN).

    Isolation:

    Article Title: Serum Autoantibodies in Chronic Prostate Inflammation in Prostate Cancer Patients
    Article Snippet: .. Since the frequency of detected SPOP mutations was significantly lower than the one reported in the literature [ ], results were confirmed by the method originally described by Blattner et al. [ ], DNA was isolated from benign and malignant areas of frozen tissue sections using the AllPrep DNA/RNA Micro Kit. .. After an initial pre-PCR amplification step to enrich the target regions, a high-resolution melting analysis (HRM) was carried out.

    Article Title: Intragenic sequences in the trophectoderm harbour the greatest proportion of methylation errors in day 17 bovine conceptuses generated using assisted reproductive technologies
    Article Snippet: .. Genomic DNA and total RNA were isolated from single, dissected day 17 conceptuses using the AllPrep DNA/RNA Micro Kit (Qiagen, Manchester, UK) according to the manufacturers’ guidelines. .. DNA samples were quantified using a Qubit dsDNA HS assay kit (Invitrogen™, ThermoFisher Scientific Ltd., Dublin, Ireland).

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
    Article Snippet: .. To establish the incorporation of the pIRES2-EGFP-GalNAc4S-6ST into genomic DNA, total genomic DNA was isolated from SKOV3 cells using a Qiagen AllPrep DNA/RNA micro kit (Qiagen GmbH, Hilden, Germany) according to manufacturer's specifications. .. The concentration of DNA was determined using a Nanodrop 2000c spectrophotometer (NanoDrop products, Wilmington, DE, USA).

    Article Title: Cervicovaginal bacteria are a major modulator of host inflammatory responses in the female genital tract
    Article Snippet: .. DNA was isolated from the total nucleic acid using an AllPrep DNA/RNA Micro Kit (Qiagen) and sheared (S2 sonicator, Covaris). .. DNA libraries were prepared using the NEBNext DNA Library Construction kit (New England Biolabs) and sequenced on a single paired-end 250 bp Illumina MiSeq run.

    Article Title: Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
    Article Snippet: .. Methylation Sequencing Genomic DNA was isolated and purified from skeletal muscles and thyroid glands of 90-day-old chickens using an AllPrep DNA/RNA Micro Kit (QIAGEN). .. Sodium bisulfite conversion bisulfite-sequencing PCR (BSP) was performed using the EpiTech Bisulfite Kit (QIAGEN).

    Article Title: Genome-wide DNA methylation patterns of bovine blastocysts derived from in vivo embryos subjected to in vitro culture before, during or after embryonic genome activation
    Article Snippet: .. Genomic DNA (gDNA) from four replicates (10 blastocysts per replicate) of the 2C_Flush, 8C_Flush, 16C_Flush and vivo blastocyst groups was isolated using the Allprep DNA/RNA micro kit (Qiagen, Germany) following the manufacturer’s recommendations. ..

    Article Title: Imaging genetics of FOXP2 in dyslexia
    Article Snippet: .. Total DNA and RNA were isolated using the AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany). .. Quality of total RNA of all samples was checked for degradation (RNA integrity number RIN > 7.9) via BioAnalyzer measurements (Agilent Technologies, Waldbronn, Germany).

    Article Title: Prognostic Impact of HER2 and ER Status of Circulating Tumor Cells in Metastatic Breast Cancer Patients with a HER2-Negative Primary Tumor
    Article Snippet: .. Larger than 200 bp RNA was isolated using the AllPrep DNA/RNA Micro Kit (Qiagen, Germantown, MD). .. Generation of cDNA, preamplification, and reverse transcription quantitative polymerase chain reaction to quantify the expression of ESR1 were performed as described in detail before using a validated ESR1 Taqman assay (Hs00174860 m1; Applied Biosystems, San Francisco, CA) .

    Purification:

    Article Title: Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
    Article Snippet: .. Methylation Sequencing Genomic DNA was isolated and purified from skeletal muscles and thyroid glands of 90-day-old chickens using an AllPrep DNA/RNA Micro Kit (QIAGEN). .. Sodium bisulfite conversion bisulfite-sequencing PCR (BSP) was performed using the EpiTech Bisulfite Kit (QIAGEN).

    Article Title: Maternal Experience with Predation Risk Influences Genome-Wide Embryonic Gene Expression in Threespined Sticklebacks (Gasterosteus aculeatus)
    Article Snippet: Embryo pools were homogenized and total RNA extracted from the homogenate using the AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (as in ). .. Total RNA was purified and treated with on-column DNase digestion (Qiagen).

    Sequencing:

    Article Title: Cadmium Exposure Inhibits Branching Morphogenesis and Causes Alterations Consistent With HIF-1α Inhibition in Human Primary Breast Organoids
    Article Snippet: After 7 days of growth in primary mammosphere formation conditions, we extracted RNA from the control or cadmium treated mammospheres using the AllPrep DNA/RNA micro kit (Qiagen), including an on-column DNase treatment for RNA extraction. .. We depleted ribosomal RNAs with Ribominus (Life) and prepared sequencing libraries utilizing the SMARTer Stranded RNA-Seq kit (Clontech) following the manufacturer’s recommended protocol.

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
    Article Snippet: To establish the incorporation of the pIRES2-EGFP-GalNAc4S-6ST into genomic DNA, total genomic DNA was isolated from SKOV3 cells using a Qiagen AllPrep DNA/RNA micro kit (Qiagen GmbH, Hilden, Germany) according to manufacturer's specifications. .. Primer sets covering exon-exon boundaries were designed using Primer 3 based on the reference sequence obtained from the UCSC human genome browser ( http://genome.ucsc.edu/ ), assembly February 2009 (GRCh37/hg19).

    Article Title: Chemical and Metagenomic Studies of the Lethal Black Band Disease of Corals Reveal Two Broadly Distributed, Redox-Sensitive Mixed Polyketide/Peptide Macrocycles
    Article Snippet: Identifying the Biosynthetic Gene Cluster DNA was extracted from the BBD layer or from the unicyanobacterial culture with a PowerSoil DNA isolation kit (MoBio) or Qiagen AllPrep DNA/RNA Micro kit, as previously described. .. The unassembled, quality-filtered reads are publicly available through NCBI’s Sequence Read Archive (SRA) under the BioProject ID PRJNA269585.

    Article Title: Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
    Article Snippet: .. Methylation Sequencing Genomic DNA was isolated and purified from skeletal muscles and thyroid glands of 90-day-old chickens using an AllPrep DNA/RNA Micro Kit (QIAGEN). .. Sodium bisulfite conversion bisulfite-sequencing PCR (BSP) was performed using the EpiTech Bisulfite Kit (QIAGEN).

    Construct:

    Article Title: Chemical and Metagenomic Studies of the Lethal Black Band Disease of Corals Reveal Two Broadly Distributed, Redox-Sensitive Mixed Polyketide/Peptide Macrocycles
    Article Snippet: Identifying the Biosynthetic Gene Cluster DNA was extracted from the BBD layer or from the unicyanobacterial culture with a PowerSoil DNA isolation kit (MoBio) or Qiagen AllPrep DNA/RNA Micro kit, as previously described. .. Metagenomic libraries were constructed with a TruSeq DNA sample preparation kit (Illumina, San Diego, CA, USA) and sequenced at the University of Maryland Institute for Bioscience and Biotechnology Research on an Illumina HiSeq with a 100 bp paired-end protocol.

    cDNA Library Assay:

    Article Title: Maternal Experience with Predation Risk Influences Genome-Wide Embryonic Gene Expression in Threespined Sticklebacks (Gasterosteus aculeatus)
    Article Snippet: RNA Sequencing To obtain sufficient quantity of RNA for quantitation, quality control, and template for cDNA library creation, we haphazardly selected and pooled ten embryos from each mother (n = 8 control and n = 8 experimental mothers, n = 160 embryos total). .. Embryo pools were homogenized and total RNA extracted from the homogenate using the AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (as in ).

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Genome-wide DNA methylation patterns of bovine blastocysts derived from in vivo embryos subjected to in vitro culture before, during or after embryonic genome activation
    Article Snippet: Genomic DNA (gDNA) from four replicates (10 blastocysts per replicate) of the 2C_Flush, 8C_Flush, 16C_Flush and vivo blastocyst groups was isolated using the Allprep DNA/RNA micro kit (Qiagen, Germany) following the manufacturer’s recommendations. .. Briefly, the gDNA of each group was subjected to the MseI enzyme digestion and the MseLig12 (100 μM) (5′-TAA CTA GCA TGC-3′) and 0.5 μl MseLig21 (100 μM) (5-AGT GGG ATT CCG CAT GCT AGT-3′) adapters were ligated to the fragmented DNA using T4 DNA ligase in the presence of 10× One-phor-All buffer PLUS and ATP.

    Article Title: Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens
    Article Snippet: .. DNA was extracted from a 350 µl aliquot of transport medium with the AllPrep DNA/RNA Micro Kit (Qiagen GmbH, Hilden, Germany). β-actin DNA quantification was tested by real-time PCR with QuantiTect SYBRGreen PCR (Qiagen GmbH, Hilden, Germany) using the primers 5′ CCA ACC GCG AGA AGA TGA CC 3′ (forward) and 5′ GAT CTT CAT GAG GTA GTC AGT 3′ (reverse), corresponding to positions (5′) 382–617 (3′) of the human β-actin gene (Eurofins mwg Operon, Ebersberg, Germany). .. This resulted in amplification of a 236 bp fragment, possessing a molecular weight of 15.8 kD based on an assumed average molecular weight of 660 Dalton per basepair.

    Plasmid Preparation:

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
    Article Snippet: For comparative purposes we selected two clones (C5 and C6) containing an empty vector (pIRES2-EGFP(-)) and three clones (F5, F7 and F9) with full vector (pIRES2-EGFP-GalNAc4S-6ST). .. To establish the incorporation of the pIRES2-EGFP-GalNAc4S-6ST into genomic DNA, total genomic DNA was isolated from SKOV3 cells using a Qiagen AllPrep DNA/RNA micro kit (Qiagen GmbH, Hilden, Germany) according to manufacturer's specifications.

    Article Title: Innovative Approach of Non-Thermal Plasma Application for Improving the Growth Rate in Chickens
    Article Snippet: Methylation Sequencing Genomic DNA was isolated and purified from skeletal muscles and thyroid glands of 90-day-old chickens using an AllPrep DNA/RNA Micro Kit (QIAGEN). .. BSP products were purified, ligated, and transformed using the pGEM-T Easy Vector system I (Promega, Madison, WI, USA).

    Article Title: Equivalence of Self- and Staff-Collected Nasal Swabs for the Detection of Viral Respiratory Pathogens
    Article Snippet: DNA was extracted from a 350 µl aliquot of transport medium with the AllPrep DNA/RNA Micro Kit (Qiagen GmbH, Hilden, Germany). β-actin DNA quantification was tested by real-time PCR with QuantiTect SYBRGreen PCR (Qiagen GmbH, Hilden, Germany) using the primers 5′ CCA ACC GCG AGA AGA TGA CC 3′ (forward) and 5′ GAT CTT CAT GAG GTA GTC AGT 3′ (reverse), corresponding to positions (5′) 382–617 (3′) of the human β-actin gene (Eurofins mwg Operon, Ebersberg, Germany). .. Serial dilutions of the plasmid eTC GFP β-actin ΔZip (Plasmid 27124 by Addgene, 1 Kendall Sq.

    TaqMan Assay:

    Article Title: Prognostic Impact of HER2 and ER Status of Circulating Tumor Cells in Metastatic Breast Cancer Patients with a HER2-Negative Primary Tumor
    Article Snippet: Larger than 200 bp RNA was isolated using the AllPrep DNA/RNA Micro Kit (Qiagen, Germantown, MD). .. Generation of cDNA, preamplification, and reverse transcription quantitative polymerase chain reaction to quantify the expression of ESR1 were performed as described in detail before using a validated ESR1 Taqman assay (Hs00174860 m1; Applied Biosystems, San Francisco, CA) .

    SYBR Green Assay:

    Article Title: Sonic Hedgehog Ligand: A Role in Formation of a Mesenchymal Niche in Human Pancreatic Ductal Adenocarcinoma
    Article Snippet: RNA Extraction, cDNA Synthesis, and Gene Expression Analysis Total RNA was extracted from cells using TRI reagent (Sigma-Aldrich), and from tissue samples using the AllPrep DNA/RNA Micro kit (Qiagen, Crawley, UK). .. Gene expression analysis was performed by q-RT-PCR using a StepOnePlus machine (ThermoFisher Scientific) using SYBR Green, 5 µM each primer, and the following conditions: Denaturation at 95 °C for 10 min, followed by 40 cycles of 95 °C for 15 s and 60 °C for 60 s, and a final melt curve stage at 95 °C for 15 s, 60 °C for 1 min and 95 °C for 15 min. Primers sequences were as follows: HPRT: Sense (5′-ATTATGCTGAGGATTTGGAAAGGG), antisense (5′-GCCTCCCATCTCCTTCATCAC); SHH : Sense (5′-CGACCGCAGCAAGTACGGCA), antisense (5′-CGATTTGGCCGCCACCGAGT); ACTA2 : Sense (5′-AACATGGCATCATCACCAACTG), antisense (5′-GCAACACGAAGCTCATTGTAGAA); POSTN : Sense (5′-ACACACCCGTGAGGAAGTTGC), antisense (5′-TGAGAACGACCTTCCCTTAATCGTC).

    RNA Extraction:

    Article Title: Cadmium Exposure Inhibits Branching Morphogenesis and Causes Alterations Consistent With HIF-1α Inhibition in Human Primary Breast Organoids
    Article Snippet: .. After 7 days of growth in primary mammosphere formation conditions, we extracted RNA from the control or cadmium treated mammospheres using the AllPrep DNA/RNA micro kit (Qiagen), including an on-column DNase treatment for RNA extraction. .. RNA concentration and quality was determined using a Nanodrop (Thermo) and Bioanalyzer (Agilent).

    Article Title: Sonic Hedgehog Ligand: A Role in Formation of a Mesenchymal Niche in Human Pancreatic Ductal Adenocarcinoma
    Article Snippet: .. RNA Extraction, cDNA Synthesis, and Gene Expression Analysis Total RNA was extracted from cells using TRI reagent (Sigma-Aldrich), and from tissue samples using the AllPrep DNA/RNA Micro kit (Qiagen, Crawley, UK). .. RNA samples were reverse transcribed into cDNA using Superscript II Reverse Transcriptase (ThermoFisher Scientific).

    Sample Prep:

    Article Title: Intragenic sequences in the trophectoderm harbour the greatest proportion of methylation errors in day 17 bovine conceptuses generated using assisted reproductive technologies
    Article Snippet: Paragraph title: Sample preparation ... Genomic DNA and total RNA were isolated from single, dissected day 17 conceptuses using the AllPrep DNA/RNA Micro Kit (Qiagen, Manchester, UK) according to the manufacturers’ guidelines.

    Article Title: Chemical and Metagenomic Studies of the Lethal Black Band Disease of Corals Reveal Two Broadly Distributed, Redox-Sensitive Mixed Polyketide/Peptide Macrocycles
    Article Snippet: Identifying the Biosynthetic Gene Cluster DNA was extracted from the BBD layer or from the unicyanobacterial culture with a PowerSoil DNA isolation kit (MoBio) or Qiagen AllPrep DNA/RNA Micro kit, as previously described. .. Metagenomic libraries were constructed with a TruSeq DNA sample preparation kit (Illumina, San Diego, CA, USA) and sequenced at the University of Maryland Institute for Bioscience and Biotechnology Research on an Illumina HiSeq with a 100 bp paired-end protocol.

    Quantitation Assay:

    Article Title: Maternal Experience with Predation Risk Influences Genome-Wide Embryonic Gene Expression in Threespined Sticklebacks (Gasterosteus aculeatus)
    Article Snippet: RNA Sequencing To obtain sufficient quantity of RNA for quantitation, quality control, and template for cDNA library creation, we haphazardly selected and pooled ten embryos from each mother (n = 8 control and n = 8 experimental mothers, n = 160 embryos total). .. Embryo pools were homogenized and total RNA extracted from the homogenate using the AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (as in ).

    Spectrophotometry:

    Article Title: Translational study identifies XPF and MUS81 as predictive biomarkers for oxaliplatin-based peri-operative chemotherapy in patients with esophageal adenocarcinoma
    Article Snippet: RNA was extracted using the Qiagen AllPrep DNA/RNA Micro Kit (Qiagen Inc., Valencia, CA) following the manufacturer’s instructions. .. RNA purity was verified via NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA).

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
    Article Snippet: To establish the incorporation of the pIRES2-EGFP-GalNAc4S-6ST into genomic DNA, total genomic DNA was isolated from SKOV3 cells using a Qiagen AllPrep DNA/RNA micro kit (Qiagen GmbH, Hilden, Germany) according to manufacturer's specifications. .. The concentration of DNA was determined using a Nanodrop 2000c spectrophotometer (NanoDrop products, Wilmington, DE, USA).

    Article Title: Maternal Experience with Predation Risk Influences Genome-Wide Embryonic Gene Expression in Threespined Sticklebacks (Gasterosteus aculeatus)
    Article Snippet: Embryo pools were homogenized and total RNA extracted from the homogenate using the AllPrep DNA/RNA Micro Kit (Qiagen, Hilden, Germany) according to the manufacturer's instructions (as in ). .. RNA was quantified on a Nanodrop spectrophotometer (Thermo Scientific, Hudson, NH) and quality verified on an Agilent Bioanalyzer (Agilent Technologies, Palo Alto, CA).

    Concentration Assay:

    Article Title: Cadmium Exposure Inhibits Branching Morphogenesis and Causes Alterations Consistent With HIF-1α Inhibition in Human Primary Breast Organoids
    Article Snippet: After 7 days of growth in primary mammosphere formation conditions, we extracted RNA from the control or cadmium treated mammospheres using the AllPrep DNA/RNA micro kit (Qiagen), including an on-column DNase treatment for RNA extraction. .. RNA concentration and quality was determined using a Nanodrop (Thermo) and Bioanalyzer (Agilent).

    Article Title: Primary Ovarian Carcinomas and Abdominal Metastasis Contain 4,6-Disulfated Chondroitin Sulfate Rich Regions, Which Provide Adhesive Properties to Tumour Cells
    Article Snippet: To establish the incorporation of the pIRES2-EGFP-GalNAc4S-6ST into genomic DNA, total genomic DNA was isolated from SKOV3 cells using a Qiagen AllPrep DNA/RNA micro kit (Qiagen GmbH, Hilden, Germany) according to manufacturer's specifications. .. The concentration of DNA was determined using a Nanodrop 2000c spectrophotometer (NanoDrop products, Wilmington, DE, USA).

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    Qiagen allprep dna rna micro kit
    Allprep Dna Rna Micro Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen allprep dna rna mirna universal kit
    Allprep Dna Rna Mirna Universal Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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