alkaline phosphatase strepavidin conjugate  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    Streptavidin Alkaline Phosphatase conjugated
    Description:
    Alkaline Phosphatase Streptavidin Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications
    Catalog Number:
    sa-5100
    Price:
    None
    Size:
    1 ml
    Category:
    Proteins
    Buy from Supplier


    Structured Review

    Vector Laboratories alkaline phosphatase strepavidin conjugate
    Streptavidin Alkaline Phosphatase conjugated
    Alkaline Phosphatase Streptavidin Avidin and streptavidin reagents are powerful tools to detect or purify biotinylated proteins nucleic acids and other macromolecules Vector Laboratories enzyme conjugated avidin and streptavidin are produced with the highest specific activity enzymes in optimal ratios Specific covalent linkages are chosen to provide stable highly active conjugates These enzyme conjugates are suitable for use in solid phase assays tissue cell staining systems and blotting applications
    https://www.bioz.com/result/alkaline phosphatase strepavidin conjugate/product/Vector Laboratories
    Average 98 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase strepavidin conjugate - by Bioz Stars, 2020-09
    98/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Detection of oligoclonal IgG kappa and IgG lambda bands in cerebrospinal fluid and serum with Hevylite(TM) antibodies. comparison with the free light chain oligoclonal pattern
    Article Snippet: .. Next, alkaline phosphatase streptavidin (Vector Laboratories Inc., Burlingame, USA, Cat. No. SA-5100) diluted 1/900, was applied for 50 min. After washing once in TBS, twice in TBST, and twice in TBS, colour reaction was performed using BCIP/NBT Kit (Vector Laboratories Inc., Cat. No. SK-5400) until suitable staining developed (15-25 min). ..

    Incubation:

    Article Title: In vivo production of interferon ? by human Tenon's fibroblasts; a possible mediator for the development of chronic conjunctival inflammation
    Article Snippet: .. The sections were then incubated with 50 μl of the streptavidin-alkaline phosphatase third layer (SA-5100, Vector Laboratories) diluted 1/100 in PBS+0.1% BSA for 1 hour at room temperature. ..

    Article Title: An expression system for screening of proteins for glycan and protein interactions
    Article Snippet: .. Blots were blocked in casein solution and incubated with biotin goat anti-GFP Ab (10 μg/ml, Vector Labs) followed by a preformed complex between streptavidin and biotinylated alkaline phosphatase (Vectastain ABC–Amp, Vector Labs). .. The chromogenic substrate for alkaline phosphatase, 5-bromo-4-chloro-3-indolyl phosphate/nitroblue tetrazolium, was provided by the DuoLuX detection kit (Vector Labs).

    Article Title: Differential expression and regulation of the non-integrin 37/67-kDa laminin receptor on peripheral blood leukocytes of healthy individuals and patients with rheumatoid arthritis
    Article Snippet: .. The following day slides were washed three times with TBS and incubated with 0.5 µg/mL of biotinylated goat anti-rabbit IgG (Dako) for 2 h at room temperature, washed three times, and incubated with streptavidin-alkaline phosphatase conjugate (Vectastain AP, Vector Labs) according to the manufacturer’s instructions for 45 min at room temperature. .. The slides were washed three times in TBS, washed once in 0.1 M TRIS pH8.2 for 5 min and finally treated with alkaline phosphatase substrate (Vector Red, Vector Labs) at room temperature for 10–20 min.

    Labeling:

    Article Title: Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)
    Article Snippet: .. However the protocol changes at the peroxidase substrate step (6.11) in that instead of the horseradish peroxidase conjugated-strepavidin, an alkaline phosphatase strepavidin conjugate is employed The second labeling is achieved by an additional antigen retrieval step in the microwave as described above plus a second immunostaining with a monoclonal antibody to the cellular protein found to be dysregulated (Vector Laboratories, Burlingame, CA). .. Cells are subsequently washed, and then incubated with a biotinylated goat anti-mouse/rabbit IgG antibody (DAKO) at a 1:100 dilution followed by an alkaline phosphatase-strepavidin conjugate (Vector Labs, Burlingame, CA) at 1:500 dilution.

    Immunostaining:

    Article Title: Identifying Dysregulated Genes Induced by Kaposi's Sarcoma-associated Herpesvirus (KSHV)
    Article Snippet: .. However the protocol changes at the peroxidase substrate step (6.11) in that instead of the horseradish peroxidase conjugated-strepavidin, an alkaline phosphatase strepavidin conjugate is employed The second labeling is achieved by an additional antigen retrieval step in the microwave as described above plus a second immunostaining with a monoclonal antibody to the cellular protein found to be dysregulated (Vector Laboratories, Burlingame, CA). .. Cells are subsequently washed, and then incubated with a biotinylated goat anti-mouse/rabbit IgG antibody (DAKO) at a 1:100 dilution followed by an alkaline phosphatase-strepavidin conjugate (Vector Labs, Burlingame, CA) at 1:500 dilution.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Vector Laboratories streptavidin alkaline phosphatase conjugate
    2G12 inhibits DC-SIGN-gp120 interaction in ELISA . The ability of mAbs to inhibit DC-SIGN-gp120 interaction was evaluated. Fc-DC-SIGN was coated on the plate. Fixed concentrations of mAbs or mannan were then added, followed by graded concentrations of biotinylated Fc-gp120, which was detected using a <t>streptavidin-alkaline</t> phosphatase conjugate. Results are representative of two independent assays.
    Streptavidin Alkaline Phosphatase Conjugate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin alkaline phosphatase conjugate/product/Vector Laboratories
    Average 98 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    streptavidin alkaline phosphatase conjugate - by Bioz Stars, 2020-09
    98/100 stars
      Buy from Supplier

    Image Search Results


    2G12 inhibits DC-SIGN-gp120 interaction in ELISA . The ability of mAbs to inhibit DC-SIGN-gp120 interaction was evaluated. Fc-DC-SIGN was coated on the plate. Fixed concentrations of mAbs or mannan were then added, followed by graded concentrations of biotinylated Fc-gp120, which was detected using a streptavidin-alkaline phosphatase conjugate. Results are representative of two independent assays.

    Journal: Retrovirology

    Article Title: Inhibition of HIV Env binding to cellular receptors by monoclonal antibody 2G12 as probed by Fc-tagged gp120

    doi: 10.1186/1742-4690-3-39

    Figure Lengend Snippet: 2G12 inhibits DC-SIGN-gp120 interaction in ELISA . The ability of mAbs to inhibit DC-SIGN-gp120 interaction was evaluated. Fc-DC-SIGN was coated on the plate. Fixed concentrations of mAbs or mannan were then added, followed by graded concentrations of biotinylated Fc-gp120, which was detected using a streptavidin-alkaline phosphatase conjugate. Results are representative of two independent assays.

    Article Snippet: Bound gp120 was then detected using streptavidin-alkaline phosphatase conjugate (Vector Laboratories, Burlingame, CA).

    Techniques: Enzyme-linked Immunosorbent Assay