alkaline phosphatase alp  (Worthington Biochemical)


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    Worthington Biochemical alkaline phosphatase alp
    Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and <t>ALP</t> together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and <t>VPH</t> were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.
    Alkaline Phosphatase Alp, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alkaline phosphatase alp/product/Worthington Biochemical
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase alp - by Bioz Stars, 2020-09
    92/100 stars

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    1) Product Images from "Development of a Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Method for Analysis of Stable 4-Hydroxyequilenin-DNA Adducts in Human Breast Cancer Cells"

    Article Title: Development of a Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Method for Analysis of Stable 4-Hydroxyequilenin-DNA Adducts in Human Breast Cancer Cells

    Journal: Chemical research in toxicology

    doi: 10.1021/tx900063g

    Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and ALP together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and VPH were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.
    Figure Legend Snippet: Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and ALP together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and VPH were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.

    Techniques Used: Incubation, ALP Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry

    Related Articles

    ALP Assay:

    Article Title: Development of a Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Method for Analysis of Stable 4-Hydroxyequilenin-DNA Adducts in Human Breast Cancer Cells
    Article Snippet: .. Alkaline phosphatase (ALP) and venom phosphodiesterase I (VPH) were purchased from Worthington (Lakewood, NJ). .. 4-OHEN was synthesized by treating equilin with Fremy’s salt as described previously ( ) with minor modifications ( ).

    Article Title: Ultrasensitive aptamer-based protein detection via a dual amplified biocatalytic strategy
    Article Snippet: .. Alkaline phosphatase (ALP), streptavidin conjugated ALP, diaphorase (DI) were obtained from Worthington Biochemical Corp. (Lakewood, NJ, USA). .. Shortened carboxylate SWCNTs and p -aminophenylphosphate ( p -APP) were supplied by Shenzhen Nanotech Port Co., Ltd. (Shenzhen, China) and LKT Laboratories, Inc. (St. Paul, MN, USA) respectively.

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  • 92
    Worthington Biochemical alkaline phosphatase alp
    Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and <t>ALP</t> together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and <t>VPH</t> were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.
    Alkaline Phosphatase Alp, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alkaline phosphatase alp/product/Worthington Biochemical
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    alkaline phosphatase alp - by Bioz Stars, 2020-09
    92/100 stars
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    Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and ALP together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and VPH were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.

    Journal: Chemical research in toxicology

    Article Title: Development of a Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry Method for Analysis of Stable 4-Hydroxyequilenin-DNA Adducts in Human Breast Cancer Cells

    doi: 10.1021/tx900063g

    Figure Lengend Snippet: Optimization of hydrolysis conditions for detecting stable 4-OHEN-DNA adducts. A) Method I, incubation with NP1 and ALP together in 25 mM ammonium acetate (pH 5.3) for 45 min at 37 °C. Method II, the DNA solutions were incubated with NP1 for 4 h in ammonium acetate at 55 °C, and then ALP and VPH were added to mixture for 4 h at 37 °C after pH adjustment to 9.8 by 0.1 M diethanolamine. B) Equal amounts of calf thymus DNA were hydrolyzed using Method I and Method II. The hydrolysates were analyzed by LC-MS/MS and compared by total relative intensity of each adduct peak.

    Article Snippet: Alkaline phosphatase (ALP) and venom phosphodiesterase I (VPH) were purchased from Worthington (Lakewood, NJ).

    Techniques: Incubation, ALP Assay, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry