alexa fluorescence conjugated secondary abs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher alexa fluorescence conjugated secondary abs
    CpG-ODN induces the co-localization of DNA-PKcs with <t>TRAF6.</t> WT, DNA-PKcs −/− and TLR9 −/− DCs were seeded in an 8-well chamber slide, and then treated with CpG (5 µg/ml) for the indicated time points. The cells were fixed, permeabilized and immunostained with anti-DNA-PKcs Ab <t>(primary)/Alexa</t> 488 (2 nd Ab) (green) and anti-TRAF6 Ab (primary)/Alexa 594 (2 nd Ab) (red). Yellow color indicates co-localization of DNA-PKcs and TRAF6. The cells were observed under an IX81 Olympus microscope with 60× oil objective powered by 1.6× magnification. The images were recorded by an ORCA R2 CCD mono camera and analyzed by the Metamorph advanced for imaging software. Similar results were obtained from at least three independent experiments.
    Alexa Fluorescence Conjugated Secondary Abs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluorescence conjugated secondary abs/product/Thermo Fisher
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    alexa fluorescence conjugated secondary abs - by Bioz Stars, 2022-10
    93/100 stars

    Images

    1) Product Images from "Involvement of DNA-PKcs in the IL-6 and IL-12 Response to CpG-ODN Is Mediated by Its Interaction with TRAF6 in Dendritic Cells"

    Article Title: Involvement of DNA-PKcs in the IL-6 and IL-12 Response to CpG-ODN Is Mediated by Its Interaction with TRAF6 in Dendritic Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0058072

    CpG-ODN induces the co-localization of DNA-PKcs with TRAF6. WT, DNA-PKcs −/− and TLR9 −/− DCs were seeded in an 8-well chamber slide, and then treated with CpG (5 µg/ml) for the indicated time points. The cells were fixed, permeabilized and immunostained with anti-DNA-PKcs Ab (primary)/Alexa 488 (2 nd Ab) (green) and anti-TRAF6 Ab (primary)/Alexa 594 (2 nd Ab) (red). Yellow color indicates co-localization of DNA-PKcs and TRAF6. The cells were observed under an IX81 Olympus microscope with 60× oil objective powered by 1.6× magnification. The images were recorded by an ORCA R2 CCD mono camera and analyzed by the Metamorph advanced for imaging software. Similar results were obtained from at least three independent experiments.
    Figure Legend Snippet: CpG-ODN induces the co-localization of DNA-PKcs with TRAF6. WT, DNA-PKcs −/− and TLR9 −/− DCs were seeded in an 8-well chamber slide, and then treated with CpG (5 µg/ml) for the indicated time points. The cells were fixed, permeabilized and immunostained with anti-DNA-PKcs Ab (primary)/Alexa 488 (2 nd Ab) (green) and anti-TRAF6 Ab (primary)/Alexa 594 (2 nd Ab) (red). Yellow color indicates co-localization of DNA-PKcs and TRAF6. The cells were observed under an IX81 Olympus microscope with 60× oil objective powered by 1.6× magnification. The images were recorded by an ORCA R2 CCD mono camera and analyzed by the Metamorph advanced for imaging software. Similar results were obtained from at least three independent experiments.

    Techniques Used: Microscopy, Imaging, Software

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    Thermo Fisher alexa fluor 488 conjugated secondary antibody
    Nrac localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) Nrac-GFP and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an <t>Alexa</t> Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.
    Alexa Fluor 488 Conjugated Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Price from $9.99 to $1999.99
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    90
    Thermo Fisher alexa fluor 488 conjugated anti mouse secondary antibody
    ASFV pE66L inhibits host protein synthesis at the translation stage. (A) The effect of overexpressed pE66L on Rluc mRNA production was determined using qRT-PCR when the cells were treated with or without cycloheximide (CHX) at 12 and 24 h. Error bars show the SD of the results from three independent experiments; ns, not significant. (B) HEK-293T cells were transfected with an empty vector or E66L vector for 24 h and then ER were isolated. Proteins were separated by SDS-PAGE and evaluated by Western blotting. (C and D) Subcellular localization of E66L and E66L(13–34). HEK-293T and IPI-2I cells were seeded on slides in 24-well plates and cotransfected with pDsRed2-ER and one of the following plasmids: PCAGGS, PCAGGS-E66L-HA, or PCAGGS-E66L(13–34)-HA (none of which encoded a protein), E66L, or E66L(13–34). At 24 h posttransfection, the cells were fixed and permeabilized with Triton X-100. The cells were then incubated with a mouse anti-HA MAb for 1 h, followed by incubation with an <t>Alexa</t> Fluor 488-conjugated goat anti-mouse (green) secondary antibody to visualize E66L or E66L(13–34). The ER protein was fused with an ER targeting sequence (Clontech) and directly visualized (red). Nuclei (blue) were stained with DAPI. The images were collected using a Zeiss LSM-510META confocal laser scanning microscope and processed with the LSM image browser (Zeiss). The white arrows indicate the cosubcellular localization between pE66L or pE66L(13–34) and pDsRed2-ER in the cells. Scale bars 5 μm.
    Alexa Fluor 488 Conjugated Anti Mouse Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher donkey anti mouse alexa fluor 568 conjugate
    In situ fluorescence microspectroscopy analysis of the Anti-CD206 (1, 2, 3) antibodies in IR-ASC muscle. Emission spectra of Anti-CD206 <t>(1)/Alexa</t> Fluor 488 (turquoise line), Anti-CD206 (2)/Alexa Fluor 568 (red line) and Anti-CD206 (3)/Alexa Fluor 647 (yellow line).
    Donkey Anti Mouse Alexa Fluor 568 Conjugate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/donkey anti mouse alexa fluor 568 conjugate/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
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    donkey anti mouse alexa fluor 568 conjugate - by Bioz Stars, 2022-10
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    Image Search Results


    Nrac localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) Nrac-GFP and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.

    Journal: PLoS ONE

    Article Title: Nrac, a Novel Nutritionally-Regulated Adipose and Cardiac-Enriched Gene

    doi: 10.1371/journal.pone.0046254

    Figure Lengend Snippet: Nrac localizes to the plasma membrane. A) Predicted transmembrane domains. Fluorescence imaging of HEK293 cells transfected with plasmids encoding B) LCK-tRFP and C) Nrac-GFP and D) Merged picture. E) Immunostaining with a primary antibody against Nrac, visualized by an Alexa Fluor 488-conjugated secondary antibody, F) Hoechst staining for nuclei, and G) merged picture, in paraffin-embedded sections of mouse white adipose tissue.

    Article Snippet: An antibody against Nrac was from Santa Cruz Biotechnology (Santa Cruz, CA), and the Nrac immunostaining with visualized by an Alexa Fluor 488-conjugated secondary antibody (Invitrogen, Grand Island, NY).

    Techniques: Fluorescence, Imaging, Transfection, Immunostaining, Staining

    Impact of B. microti infection on spleen of the infected mice. (A) B. microti infection resulted in a significant increase in spleen sizes of mice compared to uninfected, naïve mice. (B) White and red pulp zones were significantly enlarged while demarcation zone was not apparent in the spleen of B. microti infected mice. Numbers of lysed erythrocytes (marked by arrowheads) as well as various free parasitic forms (marked by arrows) were also observed in infected mouse spleen. (C) In IFA conducted the spleen section, red color indicates auto-fluorescence of RBCs, blue shows nuclear staining of cells while green fluorescence marks B. microti probed with infected human plasma followed by detection with Alexa fluor 488 conjugated secondary antibodies. Apparent lysed infected erythrocytes presence is marked by a circle. Several free, released parasitic forms were also detected (marked by arrows) among erythrocytes. Bars represent 25 μm.

    Journal: Frontiers in Microbiology

    Article Title: Babesia microti Infection Changes Host Spleen Architecture and Is Cleared by a Th1 Immune Response

    doi: 10.3389/fmicb.2018.00085

    Figure Lengend Snippet: Impact of B. microti infection on spleen of the infected mice. (A) B. microti infection resulted in a significant increase in spleen sizes of mice compared to uninfected, naïve mice. (B) White and red pulp zones were significantly enlarged while demarcation zone was not apparent in the spleen of B. microti infected mice. Numbers of lysed erythrocytes (marked by arrowheads) as well as various free parasitic forms (marked by arrows) were also observed in infected mouse spleen. (C) In IFA conducted the spleen section, red color indicates auto-fluorescence of RBCs, blue shows nuclear staining of cells while green fluorescence marks B. microti probed with infected human plasma followed by detection with Alexa fluor 488 conjugated secondary antibodies. Apparent lysed infected erythrocytes presence is marked by a circle. Several free, released parasitic forms were also detected (marked by arrows) among erythrocytes. Bars represent 25 μm.

    Article Snippet: Staining with 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI) was included to stain nucleus with the anti-human secondary antibody conjugated to Alexa fluor 488 (Molecular Probes).

    Techniques: Infection, Mouse Assay, Immunofluorescence, Fluorescence, Staining

    ASFV pE66L inhibits host protein synthesis at the translation stage. (A) The effect of overexpressed pE66L on Rluc mRNA production was determined using qRT-PCR when the cells were treated with or without cycloheximide (CHX) at 12 and 24 h. Error bars show the SD of the results from three independent experiments; ns, not significant. (B) HEK-293T cells were transfected with an empty vector or E66L vector for 24 h and then ER were isolated. Proteins were separated by SDS-PAGE and evaluated by Western blotting. (C and D) Subcellular localization of E66L and E66L(13–34). HEK-293T and IPI-2I cells were seeded on slides in 24-well plates and cotransfected with pDsRed2-ER and one of the following plasmids: PCAGGS, PCAGGS-E66L-HA, or PCAGGS-E66L(13–34)-HA (none of which encoded a protein), E66L, or E66L(13–34). At 24 h posttransfection, the cells were fixed and permeabilized with Triton X-100. The cells were then incubated with a mouse anti-HA MAb for 1 h, followed by incubation with an Alexa Fluor 488-conjugated goat anti-mouse (green) secondary antibody to visualize E66L or E66L(13–34). The ER protein was fused with an ER targeting sequence (Clontech) and directly visualized (red). Nuclei (blue) were stained with DAPI. The images were collected using a Zeiss LSM-510META confocal laser scanning microscope and processed with the LSM image browser (Zeiss). The white arrows indicate the cosubcellular localization between pE66L or pE66L(13–34) and pDsRed2-ER in the cells. Scale bars 5 μm.

    Journal: Journal of Virology

    Article Title: Novel Function of African Swine Fever Virus pE66L in Inhibition of Host Translation by the PKR/eIF2α Pathway

    doi: 10.1128/JVI.01872-20

    Figure Lengend Snippet: ASFV pE66L inhibits host protein synthesis at the translation stage. (A) The effect of overexpressed pE66L on Rluc mRNA production was determined using qRT-PCR when the cells were treated with or without cycloheximide (CHX) at 12 and 24 h. Error bars show the SD of the results from three independent experiments; ns, not significant. (B) HEK-293T cells were transfected with an empty vector or E66L vector for 24 h and then ER were isolated. Proteins were separated by SDS-PAGE and evaluated by Western blotting. (C and D) Subcellular localization of E66L and E66L(13–34). HEK-293T and IPI-2I cells were seeded on slides in 24-well plates and cotransfected with pDsRed2-ER and one of the following plasmids: PCAGGS, PCAGGS-E66L-HA, or PCAGGS-E66L(13–34)-HA (none of which encoded a protein), E66L, or E66L(13–34). At 24 h posttransfection, the cells were fixed and permeabilized with Triton X-100. The cells were then incubated with a mouse anti-HA MAb for 1 h, followed by incubation with an Alexa Fluor 488-conjugated goat anti-mouse (green) secondary antibody to visualize E66L or E66L(13–34). The ER protein was fused with an ER targeting sequence (Clontech) and directly visualized (red). Nuclei (blue) were stained with DAPI. The images were collected using a Zeiss LSM-510META confocal laser scanning microscope and processed with the LSM image browser (Zeiss). The white arrows indicate the cosubcellular localization between pE66L or pE66L(13–34) and pDsRed2-ER in the cells. Scale bars 5 μm.

    Article Snippet: After three washes with PBS, the cells were blocked with PBS containing 2% bovine serum albumin for 1 h and then incubated with MAbs against HA protein for 1 h. The cells were rinsed with PBS and then treated with an Alexa Fluor 488-conjugated anti-mouse secondary antibody (Thermo Fisher Scientific, USA) for 1 h. The fluorescence images were visualized using an inverted fluorescence microscope (Olympus IX73).

    Techniques: Quantitative RT-PCR, Transfection, Plasmid Preparation, Isolation, SDS Page, Western Blot, Incubation, Sequencing, Staining, Laser-Scanning Microscopy

    In situ fluorescence microspectroscopy analysis of the Anti-CD206 (1, 2, 3) antibodies in IR-ASC muscle. Emission spectra of Anti-CD206 (1)/Alexa Fluor 488 (turquoise line), Anti-CD206 (2)/Alexa Fluor 568 (red line) and Anti-CD206 (3)/Alexa Fluor 647 (yellow line).

    Journal: Genes

    Article Title: Non-Specific Binding, a Limitation of the Immunofluorescence Method to Study Macrophages In Situ

    doi: 10.3390/genes12050649

    Figure Lengend Snippet: In situ fluorescence microspectroscopy analysis of the Anti-CD206 (1, 2, 3) antibodies in IR-ASC muscle. Emission spectra of Anti-CD206 (1)/Alexa Fluor 488 (turquoise line), Anti-CD206 (2)/Alexa Fluor 568 (red line) and Anti-CD206 (3)/Alexa Fluor 647 (yellow line).

    Article Snippet: Sections were washed for 20 min in PBS and incubated with either the secondary donkey anti-rabbit Alexa Fluor 488 conjugate (Thermo Scientific, A-21206) at 1:500 dilution, the secondary donkey anti-mouse Alexa Fluor 568 conjugate (Thermo Scientific, A10037) at 1:500 dilution, or the secondary donkey anti-goat Alexa Fluor 647 conjugate (Abcam, ab150131) at 1:500 dilution for two hours at room temperature and rinsed.

    Techniques: In Situ, Fluorescence