Alexa Fluorescence Conjugated Secondary Abs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 2 article reviews
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1) Product Images from "Involvement of DNA-PKcs in the IL-6 and IL-12 Response to CpG-ODN Is Mediated by Its Interaction with TRAF6 in Dendritic Cells"
Article Title: Involvement of DNA-PKcs in the IL-6 and IL-12 Response to CpG-ODN Is Mediated by Its Interaction with TRAF6 in Dendritic Cells
Journal: PLoS ONE
Figure Legend Snippet: CpG-ODN induces the co-localization of DNA-PKcs with TRAF6. WT, DNA-PKcs −/− and TLR9 −/− DCs were seeded in an 8-well chamber slide, and then treated with CpG (5 µg/ml) for the indicated time points. The cells were fixed, permeabilized and immunostained with anti-DNA-PKcs Ab (primary)/Alexa 488 (2 nd Ab) (green) and anti-TRAF6 Ab (primary)/Alexa 594 (2 nd Ab) (red). Yellow color indicates co-localization of DNA-PKcs and TRAF6. The cells were observed under an IX81 Olympus microscope with 60× oil objective powered by 1.6× magnification. The images were recorded by an ORCA R2 CCD mono camera and analyzed by the Metamorph advanced for imaging software. Similar results were obtained from at least three independent experiments.
Techniques Used: Microscopy, Imaging, Software