alexa fluor 647 conjugated goat anti mouse igg2a  (Thermo Fisher)


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    Structured Review

    Thermo Fisher alexa fluor 647 conjugated goat anti mouse igg2a
    Binding of IAV to pAEC and ipAEC. Primary pAECs (A) or immortalized pAECs (B) were incubated with H1N1 IL/08 (red), pH1N1 CA/09 (green) or mock virus preparation (black) at an MOI of 50 for 90 min on ice. Cells were washed either with cold DMEM (solid lines) or acid glycine buffer (dotted lines). Surface bound virus was analyzed by flowcytometry using anti-NP monoclonal antibody followed by <t>Alexa</t> <t>Fluor</t> 647 conjugated goat anti-mouse <t>IgG2a.</t> Data are representative of three independent experiments.
    Alexa Fluor 647 Conjugated Goat Anti Mouse Igg2a, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 conjugated goat anti mouse igg2a/product/Thermo Fisher
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 647 conjugated goat anti mouse igg2a - by Bioz Stars, 2020-09
    85/100 stars

    Images

    1) Product Images from "Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells"

    Article Title: Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0138704

    Binding of IAV to pAEC and ipAEC. Primary pAECs (A) or immortalized pAECs (B) were incubated with H1N1 IL/08 (red), pH1N1 CA/09 (green) or mock virus preparation (black) at an MOI of 50 for 90 min on ice. Cells were washed either with cold DMEM (solid lines) or acid glycine buffer (dotted lines). Surface bound virus was analyzed by flowcytometry using anti-NP monoclonal antibody followed by Alexa Fluor 647 conjugated goat anti-mouse IgG2a. Data are representative of three independent experiments.
    Figure Legend Snippet: Binding of IAV to pAEC and ipAEC. Primary pAECs (A) or immortalized pAECs (B) were incubated with H1N1 IL/08 (red), pH1N1 CA/09 (green) or mock virus preparation (black) at an MOI of 50 for 90 min on ice. Cells were washed either with cold DMEM (solid lines) or acid glycine buffer (dotted lines). Surface bound virus was analyzed by flowcytometry using anti-NP monoclonal antibody followed by Alexa Fluor 647 conjugated goat anti-mouse IgG2a. Data are representative of three independent experiments.

    Techniques Used: Binding Assay, Incubation

    Related Articles

    Staining:

    Article Title: Generating a Battery of Monoclonal Antibodies Against Native Green Fluorescent Protein for Immunostaining, FACS, IP, and ChIP Using a Unique Adjuvant
    Article Snippet: .. Sections were blocked with 10% normal goat serum (Sigma-Aldrich) in PBS prior to staining with 1 μg of anti-GFP antibody (either culture supernatant or purified) at 37°C for 1 h. After extensive PBS washing, preparations were incubated in Alexa Fluor 647-conjugated goat anti-mouse IgG (Life Technologies) at 37°C for 45 min. .. Sections were rinsed, mounted with Vectashield (Vector Labs), and imaged as described above.

    Incubation:

    Article Title: Differential Induction of Type I and Type III Interferons by Swine and Human Origin H1N1 Influenza A Viruses in Porcine Airway Epithelial Cells
    Article Snippet: .. To detect bound virus, cells were incubated with monoclonal antibody to influenza A nucleoprotein (NP) (MAB8800; EMD Millipore corporation, Temecula, CA) for 30 min. followed by Alexa Fluor 647 conjugated goat anti-mouse IgG2a (Molecular probes Inc, Eugene, OR) for 30 min at 4o C. The MAB detects NP from all IAV strains. .. Mouse IgG2a isotype (eBioscience, San Diego, CA) was used to control for non-specific/background fluorescence.

    Article Title: N-Glycans on the Rift Valley Fever Virus Envelope Glycoproteins Gn and Gc Redundantly Support Viral Infection via DC-SIGN
    Article Snippet: .. Cells were washed twice with permeabilization buffer, and the cells were incubated at 4 °C for 40 min with Alexa Fluor 647-conjugated goat anti-mouse IgG (Life Technologies). .. Permeabilization buffer was used to wash cells three times, and then cells were resuspended in fluorescence-activated cell sorting (FACS) buffer.

    Article Title: Generating a Battery of Monoclonal Antibodies Against Native Green Fluorescent Protein for Immunostaining, FACS, IP, and ChIP Using a Unique Adjuvant
    Article Snippet: .. Sections were blocked with 10% normal goat serum (Sigma-Aldrich) in PBS prior to staining with 1 μg of anti-GFP antibody (either culture supernatant or purified) at 37°C for 1 h. After extensive PBS washing, preparations were incubated in Alexa Fluor 647-conjugated goat anti-mouse IgG (Life Technologies) at 37°C for 45 min. .. Sections were rinsed, mounted with Vectashield (Vector Labs), and imaged as described above.

    Article Title: Probing the contribution of individual polypeptide GalNAc-transferase isoforms to the O-glycoproteome by inducible expression in isogenic cell lines
    Article Snippet: .. After washing in PBS, cells were incubated with Alexa Fluor 647–conjugated goat anti-mouse IgG (A-21235, Thermo Scientific) (1:1000 in PBS, 1% BSA) for 1 h at RT, washed, and stored in PBS, 1% BSA at 4 °C until analysis. .. Secondary-only and IgG isotype control was performed in parallel by replacing 4C4 with negative control mouse IgG1 (X0931, Dako A/S) diluted 1:100 in PBS, 1% BSA.

    Purification:

    Article Title: Generating a Battery of Monoclonal Antibodies Against Native Green Fluorescent Protein for Immunostaining, FACS, IP, and ChIP Using a Unique Adjuvant
    Article Snippet: .. Sections were blocked with 10% normal goat serum (Sigma-Aldrich) in PBS prior to staining with 1 μg of anti-GFP antibody (either culture supernatant or purified) at 37°C for 1 h. After extensive PBS washing, preparations were incubated in Alexa Fluor 647-conjugated goat anti-mouse IgG (Life Technologies) at 37°C for 45 min. .. Sections were rinsed, mounted with Vectashield (Vector Labs), and imaged as described above.

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    Thermo Fisher alexa fluor conjugated secondary antibody
    Alexa Fluor Conjugated Secondary Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor conjugated secondary antibody/product/Thermo Fisher
    Average 99 stars, based on 48 article reviews
    Price from $9.99 to $1999.99
    alexa fluor conjugated secondary antibody - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    91
    Thermo Fisher alexa fluor 647 conjugated goat anti mouse igg
    Flow cytometric quantification of induced GalNAc-T2. A , cells were induced for 48 h with doxycycline ( Dox ) before being fixed, permeabilized, and stained with anti-GalNAc-T2 primary antibody and <t>Alexa</t> <t>Fluor</t> 647 secondary antibody. B , enlargement of HEK ind T2 induced from 0 to 16 ng/ml doxycycline with a help line centered at the peak of 0 ng/ml. The induction is biphasic with a distinct global right shift of the lower-expressing population. Data are representative of two biological and two technical replicates.
    Alexa Fluor 647 Conjugated Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 conjugated goat anti mouse igg/product/Thermo Fisher
    Average 91 stars, based on 42 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 647 conjugated goat anti mouse igg - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

    99
    Thermo Fisher alexa fluor 647 conjugated donkey anti mouse secondary
    Specific binding of aptamer A5 to GLUT1 in human tissue. (A) Human brain cortical tissue was labeled with a GLUT1 <t>Alexa</t> Fluor 488-conjugated antibody, FAM-labeled aptamer A5, or FAM-labeled scrambled aptamer. (B) Human tissue was co-labeled with lectin and the GLUT1 antibody. (C) Human tissue was co-labeled with lectin and the Alexa Fluor 647-labeled aptamer A5. All immunolabeling was performed with two separate tissue samples, with three images taken from each tissue section to validate expression patterns. The representative images are prov ided with DAPI co-labeling (blue) (scale bar: 200 μm).
    Alexa Fluor 647 Conjugated Donkey Anti Mouse Secondary, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 647 conjugated donkey anti mouse secondary/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 647 conjugated donkey anti mouse secondary - by Bioz Stars, 2020-09
    99/100 stars
      Buy from Supplier

    Image Search Results


    Flow cytometric quantification of induced GalNAc-T2. A , cells were induced for 48 h with doxycycline ( Dox ) before being fixed, permeabilized, and stained with anti-GalNAc-T2 primary antibody and Alexa Fluor 647 secondary antibody. B , enlargement of HEK ind T2 induced from 0 to 16 ng/ml doxycycline with a help line centered at the peak of 0 ng/ml. The induction is biphasic with a distinct global right shift of the lower-expressing population. Data are representative of two biological and two technical replicates.

    Journal: The Journal of Biological Chemistry

    Article Title: Probing the contribution of individual polypeptide GalNAc-transferase isoforms to the O-glycoproteome by inducible expression in isogenic cell lines

    doi: 10.1074/jbc.RA118.004516

    Figure Lengend Snippet: Flow cytometric quantification of induced GalNAc-T2. A , cells were induced for 48 h with doxycycline ( Dox ) before being fixed, permeabilized, and stained with anti-GalNAc-T2 primary antibody and Alexa Fluor 647 secondary antibody. B , enlargement of HEK ind T2 induced from 0 to 16 ng/ml doxycycline with a help line centered at the peak of 0 ng/ml. The induction is biphasic with a distinct global right shift of the lower-expressing population. Data are representative of two biological and two technical replicates.

    Article Snippet: After washing in PBS, cells were incubated with Alexa Fluor 647–conjugated goat anti-mouse IgG (A-21235, Thermo Scientific) (1:1000 in PBS, 1% BSA) for 1 h at RT, washed, and stored in PBS, 1% BSA at 4 °C until analysis.

    Techniques: Flow Cytometry, Staining, Expressing

    Specific binding of aptamer A5 to GLUT1 in human tissue. (A) Human brain cortical tissue was labeled with a GLUT1 Alexa Fluor 488-conjugated antibody, FAM-labeled aptamer A5, or FAM-labeled scrambled aptamer. (B) Human tissue was co-labeled with lectin and the GLUT1 antibody. (C) Human tissue was co-labeled with lectin and the Alexa Fluor 647-labeled aptamer A5. All immunolabeling was performed with two separate tissue samples, with three images taken from each tissue section to validate expression patterns. The representative images are prov ided with DAPI co-labeling (blue) (scale bar: 200 μm).

    Journal: bioRxiv

    Article Title: CRISPR-mediated isogenic cell-SELEX approach for generating highly specific aptamers against native membrane proteins

    doi: 10.1101/2020.02.17.949768

    Figure Lengend Snippet: Specific binding of aptamer A5 to GLUT1 in human tissue. (A) Human brain cortical tissue was labeled with a GLUT1 Alexa Fluor 488-conjugated antibody, FAM-labeled aptamer A5, or FAM-labeled scrambled aptamer. (B) Human tissue was co-labeled with lectin and the GLUT1 antibody. (C) Human tissue was co-labeled with lectin and the Alexa Fluor 647-labeled aptamer A5. All immunolabeling was performed with two separate tissue samples, with three images taken from each tissue section to validate expression patterns. The representative images are prov ided with DAPI co-labeling (blue) (scale bar: 200 μm).

    Article Snippet: Antibody-incubated tissue sections were followed by treatment with Alexa Fluor 647 conjugated donkey anti-mouse secondary (Thermo Fisher, A-31573) for 1 hour.

    Techniques: Binding Assay, Labeling, Immunolabeling, Expressing