alexa fluor 594 goat anti mouse igg  (Thermo Fisher)


Bioz Verified Symbol Thermo Fisher is a verified supplier
Bioz Manufacturer Symbol Thermo Fisher manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Name:
    Alexa Fluor 594 Goat Anti Mouse SFX Kit
    Description:
    Alexa Fluor SFX Kits contain Image iT FX signal enhancer Cat no I36933 plus one of sixteen different Alexa Fluor dye labeled secondary antibodies These kits provide 400 µg 0 2 mL of 2 mg mL of either goat anti mouse IgG or goat anti rabbit IgG antibody as a standard or highly cross adsorbed preparation conjugated to Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 594 or Alexa Fluor 647 dye four of our most commonly used Alexa Fluor dyes The Alexa Fluor 594 goat anti mouse IgG highly cross adsorbed included with this kit is also available in a 1000 µg unit size Cat no A11032
    Catalog Number:
    A31624
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
    Applications:
    Antibodies and Secondary Detection|Cell Analysis
    Buy from Supplier


    Structured Review

    Thermo Fisher alexa fluor 594 goat anti mouse igg
    Alexa Fluor SFX Kits contain Image iT FX signal enhancer Cat no I36933 plus one of sixteen different Alexa Fluor dye labeled secondary antibodies These kits provide 400 µg 0 2 mL of 2 mg mL of either goat anti mouse IgG or goat anti rabbit IgG antibody as a standard or highly cross adsorbed preparation conjugated to Alexa Fluor 488 Alexa Fluor 555 Alexa Fluor 594 or Alexa Fluor 647 dye four of our most commonly used Alexa Fluor dyes The Alexa Fluor 594 goat anti mouse IgG highly cross adsorbed included with this kit is also available in a 1000 µg unit size Cat no A11032
    https://www.bioz.com/result/alexa fluor 594 goat anti mouse igg/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 594 goat anti mouse igg - by Bioz Stars, 2021-04
    97/100 stars

    Images

    Related Articles

    Labeling:

    Article Title: Dynamic movements of Ro52 cytoplasmic bodies along microtubules
    Article Snippet: The cells were first labeled with one of the following primary antibodies overnight at 10°C: mouse anti-Rpt5 (1:20,000), rabbit anti-caveolin (1:500), mouse anti-EEA1 (1:1,000), or mouse anti-LAMP2 (1:1,000). .. After washing, the cells were labeled with Alexa Fluor 594-conjugated goat anti-mouse or anti-rabbit IgG (Molecular Probes) at a dilution of 1:2,000 for 1 h at room temperature. ..

    Immunofluorescence:

    Article Title: Prothymosin ? overexpression contributes to the development of pulmonary emphysema
    Article Snippet: .. For immunofluorescence, the cells were incubated with ProT (clone 2F11) and acetylated-lysine (1:800, Cell Signaling) antibodies and subsequently incubated with Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen) and FITC-conjugated goat anti-rabbit IgG (KPL). .. The nuclei were counterstained with DAPI (Sigma-Aldrich).

    Incubation:

    Article Title: Prothymosin ? overexpression contributes to the development of pulmonary emphysema
    Article Snippet: .. For immunofluorescence, the cells were incubated with ProT (clone 2F11) and acetylated-lysine (1:800, Cell Signaling) antibodies and subsequently incubated with Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen) and FITC-conjugated goat anti-rabbit IgG (KPL). .. The nuclei were counterstained with DAPI (Sigma-Aldrich).

    Article Title: Cag Type IV Secretion System: CagI Independent Bacterial Surface Localization of CagA
    Article Snippet: .. The cells were then incubated with specific polyclonal antibody of appropriate dilutions (α-CagI-1:1000, α-CagF-1:500, α-CagT-1:1000, α-CagX-1:1000, and α-CagA-1:1000) and respective pre-immune serum (negative control) at 4° C for 2 h. Thereafter fixed cells were washed with PBS three times and were further incubated with Cy3-conjugated goat anti rabbit IgG, Alexa fluor 488 goat anti rabbit, and Alexa fluor 594 goat anti mice (Molecular probe) for 1 h at RT as required. .. The cover slips were mounted with 20% glycerol on glass slides and visualized at 100X through a Carl Zeiss fluorescence microscope equipped with oil immersion objectives.

    CtB Assay:

    Article Title: Porcine sapelovirus enters PK-15 cells via caveolae-dependent endocytosis and requires Rab7 and Rab11
    Article Snippet: 2.2 Inhibitors, antibodies and reagents All endocytic inhibitors, including chlorpromazine (CPZ), ammonium chloride (NH4 Cl), chloroquine (CQ), methyl-β-cyclodextrin (MβCD), nystatin, dynasore, 5-(N-ethyl-N-isopropyl) amiloride (EIPA), cytochalasin D (CytoD), and Jasplakinolide (Jasp) were purchased from Sigma. .. Alexa Fluor 594-conjugated cholera toxin B (CTB), Alexa Fluor 568-conjugated transferrin (Tfn) and Alexa Fluor-488 or Alexa Fluor 594-conjugated goat anti-mouse secondary antibody were purchased from Invitrogen (Carlsbad, CA, United States). .. Mouse polyclonal anti-PSV VP1 antibody was generated by our laboratory.

    Negative Control:

    Article Title: Cag Type IV Secretion System: CagI Independent Bacterial Surface Localization of CagA
    Article Snippet: .. The cells were then incubated with specific polyclonal antibody of appropriate dilutions (α-CagI-1:1000, α-CagF-1:500, α-CagT-1:1000, α-CagX-1:1000, and α-CagA-1:1000) and respective pre-immune serum (negative control) at 4° C for 2 h. Thereafter fixed cells were washed with PBS three times and were further incubated with Cy3-conjugated goat anti rabbit IgG, Alexa fluor 488 goat anti rabbit, and Alexa fluor 594 goat anti mice (Molecular probe) for 1 h at RT as required. .. The cover slips were mounted with 20% glycerol on glass slides and visualized at 100X through a Carl Zeiss fluorescence microscope equipped with oil immersion objectives.

    Mouse Assay:

    Article Title: Cag Type IV Secretion System: CagI Independent Bacterial Surface Localization of CagA
    Article Snippet: .. The cells were then incubated with specific polyclonal antibody of appropriate dilutions (α-CagI-1:1000, α-CagF-1:500, α-CagT-1:1000, α-CagX-1:1000, and α-CagA-1:1000) and respective pre-immune serum (negative control) at 4° C for 2 h. Thereafter fixed cells were washed with PBS three times and were further incubated with Cy3-conjugated goat anti rabbit IgG, Alexa fluor 488 goat anti rabbit, and Alexa fluor 594 goat anti mice (Molecular probe) for 1 h at RT as required. .. The cover slips were mounted with 20% glycerol on glass slides and visualized at 100X through a Carl Zeiss fluorescence microscope equipped with oil immersion objectives.

    other:

    Article Title: The Lysyl Oxidase Propeptide Interacts with the Receptor-Type Protein Tyrosine Phosphatase Kappa and Inhibits ?-Catenin Transcriptional Activity in Lung Cancer Cells ▿
    Article Snippet: The following antibodies were from Invitrogen: secondary anti-mouse IgG–Alexa Fluor 594 (catalog number ), secondary anti-mouse IgG–Alexa Fluor 555 (catalog number ), anti-green fluorescent protein (anti-GFP) (catalog number A-6455), and anti-V5 (catalog number R960-25).

    Fluorescence:

    Article Title: LKR/SDH Plays Important Roles throughout the Tick Life Cycle Including a Long Starvation Period
    Article Snippet: Anti-GST antibody (1∶100) was used as a negative control. .. After washing 3 times with PBS, Alexa 488- and Alexa 594-conjugated goat anti-mouse immunoglobulins (1∶1000; Molecular Probes) were applied as second antibodies at 37°C for 30 min. After three washes with PBS, samples were mounted in mounting medium (Vectashield) and then covered with a cover glass, the images were photographed recorded using a fluorescence microscope (Olympus). .. Histochemical detection of SDH activity in sectioned midgut and ovary Histochemical staining of SDH activity on tick frozen sections was based on the gel staining reaction.

    Microscopy:

    Article Title: LKR/SDH Plays Important Roles throughout the Tick Life Cycle Including a Long Starvation Period
    Article Snippet: Anti-GST antibody (1∶100) was used as a negative control. .. After washing 3 times with PBS, Alexa 488- and Alexa 594-conjugated goat anti-mouse immunoglobulins (1∶1000; Molecular Probes) were applied as second antibodies at 37°C for 30 min. After three washes with PBS, samples were mounted in mounting medium (Vectashield) and then covered with a cover glass, the images were photographed recorded using a fluorescence microscope (Olympus). .. Histochemical detection of SDH activity in sectioned midgut and ovary Histochemical staining of SDH activity on tick frozen sections was based on the gel staining reaction.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 97
    Thermo Fisher alexa fluor 594 conjugated goat anti mouse igg
    Overexpression of ProT increases protein acetylation in emphysematous lungs of mice and patients. ( a ) Immunohistochemistry (left) and quantitation (right) of acetylated-lysine (acetyl-K) in lung tissues of 4-day-old ProT HZ, HET and NT mice. ( b ) A positive correlation ( P =0.0012; Pearson’s correlation coefficient) between the levels of ProT immunoreactivity and those of acetylated-lysine immunoreactivity. ( c ) Immunohistochemistry (upper) and quantitation (lower) of acetyl-K in lung tissues from patients with emphysema of varying severity and non-COPD individuals. Values shown are immunoreactive levels in individual specimens; horizontal bars represent the mean. ( d ) Immunofluorescence microscopy. Lung tissues from patients with severe emphysema and from non-COPD individuals were incubated with mouse monoclonal antibody against ProT and rabbit polyclonal antibody against acetyl-K followed by <t>Alexa</t> Fluor 594-conjugated goat anti-mouse <t>IgG</t> and FITC-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI. Fluorescence signals for ProT (red), acetyl-K (green) and the nucleus (blue) were examined by fluorescence microscopy. ( e ) A positive correlation ( P
    Alexa Fluor 594 Conjugated Goat Anti Mouse Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 594 conjugated goat anti mouse igg/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 594 conjugated goat anti mouse igg - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher goat anti rabbit igg alexa fluor 594 conjugated antibody
    In vitro biological activity of immune sera or purified immunoglobulins from rV- neu T vaccinated mice. Panel A : ADCC elicited by sera from 10 8 pfu rV- neu T vaccinated mice. SALTO tumor cells were exposed for 2 hours to sera pooled from rV- neu T or V-wt vaccinated mice and with mononuclear effector cells derived from normal BALB/c spleens at a ratio of 50:1. Results represent average percent cytotoxicity of two independent experiments. *p ≤ 0.01. Panel B : Effects of anti-Neu Igs on SALTO cell proliferation. SALTO tumor cells following serum depletion were incubated in DMEM medium containing 0.2% BSA with or without rV- neu T or V-wt purified Igs. Relative cell numbers of triplicate experiments were determined after incubation of 72 h at 37˚C using a sulforhodamine B based proliferation assay and expressed as percent increase or decrease in comparison to vehicle control (DMEM 0.2% BSA). *p ≤ 0.01. Panel C : p185 Neu receptor down regulation by anti-Neu Igs. SALTO cells were stained with stained with rV- neu T purified Igs and then with goat anti-mouse <t>IgG</t> <t>Alexa</t> fluor-488-conjugated antibody. Original magnification x 500. Expression and phosphorylation of ERK1 and ERK2 after rV- neu T Igs chronically treatment of SALTO tumor cells. Serum depleted SALTO cells were treated with rV- neu T- and V-wt Igs and the ratio between ERK1 and ERK2 and pERK1/pERK2 expression was analyzed by western blotting. Densitometric ratios between the phosphorylated and total levels of each protein are reported. Panel D : Effects of anti-Neu Igs on SALTO cells apoptosis. SALTO cells were plated at 2.5×10 4 cells/well, incubated in DMEM medium containing 0.2% BSA with or without rV- neu T or V-wt purified Igs (10 μg/ml) and stained with a specific anti-cleaved caspase 3 antibody. Staurosporine (1 μM) treatment was used as positive control. Nuclei were counterstained with Hoechst 33342. Original magnification x400.
    Goat Anti Rabbit Igg Alexa Fluor 594 Conjugated Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti rabbit igg alexa fluor 594 conjugated antibody/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti rabbit igg alexa fluor 594 conjugated antibody - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    97
    Thermo Fisher alexa fluor 594 conjugated goat anti rabbit igg
    Extracellular histones cause fatal thromboembolism in mice. (A) The lethal effect of extracellular histones. Mice were intravenously injected with histones (0-80 µg/g, n = 7-12 per group), and survival was analyzed. (B) Histone-induced thrombocytopenia. Numbers of platelets (PLT), red blood cells (RBC), and white blood cells (WBC) in blood 10 min after infusion with histones (0-95 µg/g, n = 3-7 per group, mean ± S.D.) are shown. Data are presented as percentage of the vehicle group (0 µg/g histones). (C) Distribution of DyLight488-labeled platelets and <t>Alexa-Fluor</t> 594-labeled fibrin(ogen) in lung tissue 10 min after infusion with vehicle or 75 µg/g histones. Nuclei were stained with DAPI. Representative images of n = 4. Scale bar = 100 µm. * P
    Alexa Fluor 594 Conjugated Goat Anti Rabbit Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 594 conjugated goat anti rabbit igg/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 594 conjugated goat anti rabbit igg - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    Image Search Results


    Overexpression of ProT increases protein acetylation in emphysematous lungs of mice and patients. ( a ) Immunohistochemistry (left) and quantitation (right) of acetylated-lysine (acetyl-K) in lung tissues of 4-day-old ProT HZ, HET and NT mice. ( b ) A positive correlation ( P =0.0012; Pearson’s correlation coefficient) between the levels of ProT immunoreactivity and those of acetylated-lysine immunoreactivity. ( c ) Immunohistochemistry (upper) and quantitation (lower) of acetyl-K in lung tissues from patients with emphysema of varying severity and non-COPD individuals. Values shown are immunoreactive levels in individual specimens; horizontal bars represent the mean. ( d ) Immunofluorescence microscopy. Lung tissues from patients with severe emphysema and from non-COPD individuals were incubated with mouse monoclonal antibody against ProT and rabbit polyclonal antibody against acetyl-K followed by Alexa Fluor 594-conjugated goat anti-mouse IgG and FITC-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI. Fluorescence signals for ProT (red), acetyl-K (green) and the nucleus (blue) were examined by fluorescence microscopy. ( e ) A positive correlation ( P

    Journal: Nature Communications

    Article Title: Prothymosin ? overexpression contributes to the development of pulmonary emphysema

    doi: 10.1038/ncomms2906

    Figure Lengend Snippet: Overexpression of ProT increases protein acetylation in emphysematous lungs of mice and patients. ( a ) Immunohistochemistry (left) and quantitation (right) of acetylated-lysine (acetyl-K) in lung tissues of 4-day-old ProT HZ, HET and NT mice. ( b ) A positive correlation ( P =0.0012; Pearson’s correlation coefficient) between the levels of ProT immunoreactivity and those of acetylated-lysine immunoreactivity. ( c ) Immunohistochemistry (upper) and quantitation (lower) of acetyl-K in lung tissues from patients with emphysema of varying severity and non-COPD individuals. Values shown are immunoreactive levels in individual specimens; horizontal bars represent the mean. ( d ) Immunofluorescence microscopy. Lung tissues from patients with severe emphysema and from non-COPD individuals were incubated with mouse monoclonal antibody against ProT and rabbit polyclonal antibody against acetyl-K followed by Alexa Fluor 594-conjugated goat anti-mouse IgG and FITC-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI. Fluorescence signals for ProT (red), acetyl-K (green) and the nucleus (blue) were examined by fluorescence microscopy. ( e ) A positive correlation ( P

    Article Snippet: For immunofluorescence, the cells were incubated with ProT (clone 2F11) and acetylated-lysine (1:800, Cell Signaling) antibodies and subsequently incubated with Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen) and FITC-conjugated goat anti-rabbit IgG (KPL).

    Techniques: Over Expression, Mouse Assay, Immunohistochemistry, Quantitation Assay, Immunofluorescence, Microscopy, Incubation, Fluorescence

    Overexpression of ProT increases NF-κB acetylation in emphysematous lungs of mice and patients. ( a – c ) Immunohistochemical detection of acetyl-NF-κB p65 in lung tissues from 4-day-old ProT HZ, HET and NT mice ( a ), from patients with emphysema of varying severity and non-COPD individuals ( b ) and from FVB mice that had been intratracheally injected with lentiviral vectors expressing ProT or luciferase (Luc) shRNA followed by intraperitoneal injections of CSE or saline twice a week for 6 weeks ( c ). Note that positive nuclear staining for acetyl-NF-κB p65 in the lung epithelium of ProT transgenic mice was observed ( a ) and that higher levels of acetyl-NF-κB p65 accumulation were detected in patients with more severe emphysema ( b ). The boxed areas in upper panels (original magnification × 400; Scale bar=20 μm) are magnified and shown in lower panels. ( d , e ) Immunofluorescence microscopy. Lung tissues from ProT HET and NT mice that had been injected intraperitoneally with CSE or saline twice a week for 6 weeks ( d ) and mouse lung epithelial MLE 12 cells that had been transduced with ProT and GFP by lentiviral vectors Lenti-ProT and Lenti-GFP, respectively, and then treated with CSE or saline ( e ) were incubated with mouse monoclonal antibody against ProT and rabbit polyclonal antibody against NF-κB p65 or acetyl-NF-κB p65 (Lys310) followed by Alexa Fluor 594-conjugated goat anti-mouse IgG and Alexa Fluor 488-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI. Fluorescence signals for ProT (red), NF-κB p65 (green), acetyl-NF-κB p65 (green) and the nucleus (blue) were examined by fluorescence microscopy. Scale bar=10 μm; original magnification × 400. Results are representative of three independent experiments.

    Journal: Nature Communications

    Article Title: Prothymosin ? overexpression contributes to the development of pulmonary emphysema

    doi: 10.1038/ncomms2906

    Figure Lengend Snippet: Overexpression of ProT increases NF-κB acetylation in emphysematous lungs of mice and patients. ( a – c ) Immunohistochemical detection of acetyl-NF-κB p65 in lung tissues from 4-day-old ProT HZ, HET and NT mice ( a ), from patients with emphysema of varying severity and non-COPD individuals ( b ) and from FVB mice that had been intratracheally injected with lentiviral vectors expressing ProT or luciferase (Luc) shRNA followed by intraperitoneal injections of CSE or saline twice a week for 6 weeks ( c ). Note that positive nuclear staining for acetyl-NF-κB p65 in the lung epithelium of ProT transgenic mice was observed ( a ) and that higher levels of acetyl-NF-κB p65 accumulation were detected in patients with more severe emphysema ( b ). The boxed areas in upper panels (original magnification × 400; Scale bar=20 μm) are magnified and shown in lower panels. ( d , e ) Immunofluorescence microscopy. Lung tissues from ProT HET and NT mice that had been injected intraperitoneally with CSE or saline twice a week for 6 weeks ( d ) and mouse lung epithelial MLE 12 cells that had been transduced with ProT and GFP by lentiviral vectors Lenti-ProT and Lenti-GFP, respectively, and then treated with CSE or saline ( e ) were incubated with mouse monoclonal antibody against ProT and rabbit polyclonal antibody against NF-κB p65 or acetyl-NF-κB p65 (Lys310) followed by Alexa Fluor 594-conjugated goat anti-mouse IgG and Alexa Fluor 488-conjugated goat anti-rabbit IgG. Nuclei were counterstained with DAPI. Fluorescence signals for ProT (red), NF-κB p65 (green), acetyl-NF-κB p65 (green) and the nucleus (blue) were examined by fluorescence microscopy. Scale bar=10 μm; original magnification × 400. Results are representative of three independent experiments.

    Article Snippet: For immunofluorescence, the cells were incubated with ProT (clone 2F11) and acetylated-lysine (1:800, Cell Signaling) antibodies and subsequently incubated with Alexa Fluor 594-conjugated goat anti-mouse IgG (Invitrogen) and FITC-conjugated goat anti-rabbit IgG (KPL).

    Techniques: Over Expression, Mouse Assay, Immunohistochemistry, Injection, Expressing, Luciferase, shRNA, Staining, Transgenic Assay, Immunofluorescence, Microscopy, Transduction, Incubation, Fluorescence

    LOX-PP leads to relocalization to the lysosome and degradation of PIC. (A) H1299 cells were cotransfected with GFP-PIC and a V5-tagged LOX-PP construct or EV DNA, as control. For lysosome staining, a LAMP2 antibody and anti-mouse Alexa Fluor 555-IgG antibody

    Journal: Molecular and Cellular Biology

    Article Title: The Lysyl Oxidase Propeptide Interacts with the Receptor-Type Protein Tyrosine Phosphatase Kappa and Inhibits ?-Catenin Transcriptional Activity in Lung Cancer Cells ▿

    doi: 10.1128/MCB.01426-10

    Figure Lengend Snippet: LOX-PP leads to relocalization to the lysosome and degradation of PIC. (A) H1299 cells were cotransfected with GFP-PIC and a V5-tagged LOX-PP construct or EV DNA, as control. For lysosome staining, a LAMP2 antibody and anti-mouse Alexa Fluor 555-IgG antibody

    Article Snippet: The following antibodies were from Invitrogen: secondary anti-mouse IgG–Alexa Fluor 594 (catalog number ), secondary anti-mouse IgG–Alexa Fluor 555 (catalog number ), anti-green fluorescent protein (anti-GFP) (catalog number A-6455), and anti-V5 (catalog number R960-25).

    Techniques: Construct, Staining

    LOX-PP reduces the nuclear levels of the PIC isoform in lung cancer cells. H1299 cells were cotransfected with GFP-PIC and a V5-tagged LOX-PP construct or EV DNA as a control. For LOX-PP staining, a V5 antibody and anti-mouse Alexa Fluor 555-IgG secondary

    Journal: Molecular and Cellular Biology

    Article Title: The Lysyl Oxidase Propeptide Interacts with the Receptor-Type Protein Tyrosine Phosphatase Kappa and Inhibits ?-Catenin Transcriptional Activity in Lung Cancer Cells ▿

    doi: 10.1128/MCB.01426-10

    Figure Lengend Snippet: LOX-PP reduces the nuclear levels of the PIC isoform in lung cancer cells. H1299 cells were cotransfected with GFP-PIC and a V5-tagged LOX-PP construct or EV DNA as a control. For LOX-PP staining, a V5 antibody and anti-mouse Alexa Fluor 555-IgG secondary

    Article Snippet: The following antibodies were from Invitrogen: secondary anti-mouse IgG–Alexa Fluor 594 (catalog number ), secondary anti-mouse IgG–Alexa Fluor 555 (catalog number ), anti-green fluorescent protein (anti-GFP) (catalog number A-6455), and anti-V5 (catalog number R960-25).

    Techniques: Construct, Staining

    In vitro biological activity of immune sera or purified immunoglobulins from rV- neu T vaccinated mice. Panel A : ADCC elicited by sera from 10 8 pfu rV- neu T vaccinated mice. SALTO tumor cells were exposed for 2 hours to sera pooled from rV- neu T or V-wt vaccinated mice and with mononuclear effector cells derived from normal BALB/c spleens at a ratio of 50:1. Results represent average percent cytotoxicity of two independent experiments. *p ≤ 0.01. Panel B : Effects of anti-Neu Igs on SALTO cell proliferation. SALTO tumor cells following serum depletion were incubated in DMEM medium containing 0.2% BSA with or without rV- neu T or V-wt purified Igs. Relative cell numbers of triplicate experiments were determined after incubation of 72 h at 37˚C using a sulforhodamine B based proliferation assay and expressed as percent increase or decrease in comparison to vehicle control (DMEM 0.2% BSA). *p ≤ 0.01. Panel C : p185 Neu receptor down regulation by anti-Neu Igs. SALTO cells were stained with stained with rV- neu T purified Igs and then with goat anti-mouse IgG Alexa fluor-488-conjugated antibody. Original magnification x 500. Expression and phosphorylation of ERK1 and ERK2 after rV- neu T Igs chronically treatment of SALTO tumor cells. Serum depleted SALTO cells were treated with rV- neu T- and V-wt Igs and the ratio between ERK1 and ERK2 and pERK1/pERK2 expression was analyzed by western blotting. Densitometric ratios between the phosphorylated and total levels of each protein are reported. Panel D : Effects of anti-Neu Igs on SALTO cells apoptosis. SALTO cells were plated at 2.5×10 4 cells/well, incubated in DMEM medium containing 0.2% BSA with or without rV- neu T or V-wt purified Igs (10 μg/ml) and stained with a specific anti-cleaved caspase 3 antibody. Staurosporine (1 μM) treatment was used as positive control. Nuclei were counterstained with Hoechst 33342. Original magnification x400.

    Journal: Journal of Translational Medicine

    Article Title: Intratumoral delivery of recombinant vaccinia virus encoding for ErbB2/Neu inhibits the growth of salivary gland carcinoma cells

    doi: 10.1186/1479-5876-12-122

    Figure Lengend Snippet: In vitro biological activity of immune sera or purified immunoglobulins from rV- neu T vaccinated mice. Panel A : ADCC elicited by sera from 10 8 pfu rV- neu T vaccinated mice. SALTO tumor cells were exposed for 2 hours to sera pooled from rV- neu T or V-wt vaccinated mice and with mononuclear effector cells derived from normal BALB/c spleens at a ratio of 50:1. Results represent average percent cytotoxicity of two independent experiments. *p ≤ 0.01. Panel B : Effects of anti-Neu Igs on SALTO cell proliferation. SALTO tumor cells following serum depletion were incubated in DMEM medium containing 0.2% BSA with or without rV- neu T or V-wt purified Igs. Relative cell numbers of triplicate experiments were determined after incubation of 72 h at 37˚C using a sulforhodamine B based proliferation assay and expressed as percent increase or decrease in comparison to vehicle control (DMEM 0.2% BSA). *p ≤ 0.01. Panel C : p185 Neu receptor down regulation by anti-Neu Igs. SALTO cells were stained with stained with rV- neu T purified Igs and then with goat anti-mouse IgG Alexa fluor-488-conjugated antibody. Original magnification x 500. Expression and phosphorylation of ERK1 and ERK2 after rV- neu T Igs chronically treatment of SALTO tumor cells. Serum depleted SALTO cells were treated with rV- neu T- and V-wt Igs and the ratio between ERK1 and ERK2 and pERK1/pERK2 expression was analyzed by western blotting. Densitometric ratios between the phosphorylated and total levels of each protein are reported. Panel D : Effects of anti-Neu Igs on SALTO cells apoptosis. SALTO cells were plated at 2.5×10 4 cells/well, incubated in DMEM medium containing 0.2% BSA with or without rV- neu T or V-wt purified Igs (10 μg/ml) and stained with a specific anti-cleaved caspase 3 antibody. Staurosporine (1 μM) treatment was used as positive control. Nuclei were counterstained with Hoechst 33342. Original magnification x400.

    Article Snippet: Goat anti-mouse IgG Alexa fluor-488-conjugated antibody and goat anti-rabbit IgG Alexa fluor-594-conjugated antibody were purchased from Life Technologies™ Molecular Probes (Oregon, USA).

    Techniques: In Vitro, Activity Assay, Purification, Mouse Assay, Derivative Assay, Serum Depletion, Incubation, Proliferation Assay, Staining, Expressing, Western Blot, Positive Control

    Extracellular histones cause fatal thromboembolism in mice. (A) The lethal effect of extracellular histones. Mice were intravenously injected with histones (0-80 µg/g, n = 7-12 per group), and survival was analyzed. (B) Histone-induced thrombocytopenia. Numbers of platelets (PLT), red blood cells (RBC), and white blood cells (WBC) in blood 10 min after infusion with histones (0-95 µg/g, n = 3-7 per group, mean ± S.D.) are shown. Data are presented as percentage of the vehicle group (0 µg/g histones). (C) Distribution of DyLight488-labeled platelets and Alexa-Fluor 594-labeled fibrin(ogen) in lung tissue 10 min after infusion with vehicle or 75 µg/g histones. Nuclei were stained with DAPI. Representative images of n = 4. Scale bar = 100 µm. * P

    Journal: PLoS ONE

    Article Title: Recombinant Thrombomodulin Protects Mice against Histone-Induced Lethal Thromboembolism

    doi: 10.1371/journal.pone.0075961

    Figure Lengend Snippet: Extracellular histones cause fatal thromboembolism in mice. (A) The lethal effect of extracellular histones. Mice were intravenously injected with histones (0-80 µg/g, n = 7-12 per group), and survival was analyzed. (B) Histone-induced thrombocytopenia. Numbers of platelets (PLT), red blood cells (RBC), and white blood cells (WBC) in blood 10 min after infusion with histones (0-95 µg/g, n = 3-7 per group, mean ± S.D.) are shown. Data are presented as percentage of the vehicle group (0 µg/g histones). (C) Distribution of DyLight488-labeled platelets and Alexa-Fluor 594-labeled fibrin(ogen) in lung tissue 10 min after infusion with vehicle or 75 µg/g histones. Nuclei were stained with DAPI. Representative images of n = 4. Scale bar = 100 µm. * P

    Article Snippet: Frozen sections (4 µm) were probed with anti-fibrinogen/fibrin IgG (Dako Cytomation, Glostrup, Denmark) followed by Alexa-Fluor 594-conjugated goat anti-rabbit IgG (Invitrogen, Carlsbad, CA).

    Techniques: Mouse Assay, Injection, Labeling, Staining