alexa fluor 594 conjugated concanavalin a (Thermo Fisher)
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Name:
Concanavalin A Alexa Fluor 594 Conjugate
Description:
Concanavalin A Con A is one of the most widely used lectins in cell biology Our Alexa Fluor 594 conjugate of Con A exhibits the bright red fluorescence of the Alexa Fluor 594 dye absorption emission maxima 590 617 nm Alexa Fluor 594 Con A selectively binds to a mannopyranosyl and a glucopyranosyl residues
Catalog Number:
c11253
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None
Category:
Labeling Detection Products
Applications:
Cell Analysis|Cellular Imaging|Flow Cytometry Antibodies & Secondary Detection|General Cell Tracing|Immunofluorescence (IF)|Immunofluorescence Staining & Detection|Microbial Tracking|Flow Cytometry|Cell Tracing & Tracking
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Structured Review
Thermo Fisher
alexa fluor 594 conjugated concanavalin a
Concanavalin A Con A is one of the most widely used lectins in cell biology Our Alexa Fluor 594 conjugate of Con A exhibits the bright red fluorescence of the Alexa Fluor 594 dye absorption emission maxima 590 617 nm Alexa Fluor 594 Con A selectively binds to a mannopyranosyl and a glucopyranosyl residues
https://www.bioz.com/result/alexa fluor 594 conjugated concanavalin a/product/Thermo Fisher
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Concanavalin A Con A is one of the most widely used lectins in cell biology Our Alexa Fluor 594 conjugate of Con A exhibits the bright red fluorescence of the Alexa Fluor 594 dye absorption emission maxima 590 617 nm Alexa Fluor 594 Con A selectively binds to a mannopyranosyl and a glucopyranosyl residues
https://www.bioz.com/result/alexa fluor 594 conjugated concanavalin a/product/Thermo Fisher
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
alexa fluor 594 conjugated concanavalin a - by Bioz Stars,
2021-03
93/100 stars
Images
1) Product Images from "Membrane Binding and Subcellular Localization of Retroviral Gag Proteins Are Differentially Regulated by MA Interactions with Phosphatidylinositol-(4,5)-Bisphosphate and RNA"
Article Title: Membrane Binding and Subcellular Localization of Retroviral Gag Proteins Are Differentially Regulated by MA Interactions with Phosphatidylinositol-(4,5)-Bisphosphate and RNA
Journal: mBio
doi: 10.1128/mBio.02202-14
Figure Legend Snippet: PM Localization of HTLV-1 MA GagLZ, MLV MA GagLZ, and HERV-K MA GagLZ persists upon 5ptaseIV overexpression, unlike that of HIV-1 GagLZ and RSV MA GagLZ. (A) Schematic illustrations of HIV-1 Gag, HIV-1 GagLZ, RSV MA GagLZ, HTLV-1 MA GagLZ, MLV MA GagLZ, and HERV-K MA GagLZ are shown. The first amino acid, methionine, is included in the numbering of MA residues, although the methionine is removed upon N-terminal myristylation. All Gag proteins were expressed from the same CMV-promoter-driven vector backbone. (B) HeLa cells expressing YFP-tagged HIV-1 GagLZ, RSV MA GagLZ, HTLV-1 MA GagLZ, MLV MA GagLZ, or HERV-K MA GagLZ, along with Myc-tagged full-length (FL) 5ptaseIV or the Δ1 derivative, were stained with ConA labeled with Alexa Fluor 594 (not shown), immunostained with mouse monoclonal anti-Myc antibody and anti-mouse IgG conjugated with Alexa Fluor 647 (anti-Myc), and analyzed using a confocal fluorescence microscope. Note that overexpression of 5ptaseIV FL induced mislocalization of HIV-1 GagLZ and RSV MA GagLZ to the cytosol and abolished PM localization. In contrast, 5ptaseIV FL overexpression did not drastically alter localization of HTLV-1 MA GagLZ and MLV MA GagLZ to the PM and intracellular compartments. Overexpression of 5ptaseIV increased the localization of HERV-K MA GagLZ to intracellular compartments, but PM localization persisted regardless of the intracellular localization. (C) HeLa cells expressing YFP-tagged HIV-1 Gag, HTMA Gag, and HIV-1 (1GA) Gag were stained with ConA labeled with Alexa Fluor 594. Cells were then fixed and analyzed using a confocal fluorescence microscope. (D) Pearson’s correlation coefficients (PCC) for colocalization of Gag-YFP or GagLZ-YFP with ConA were calculated and are shown as means ± SEM. Twenty to fifty cells were analyzed per condition. **, P
Techniques Used: Over Expression, Plasmid Preparation, Expressing, Staining, Labeling, Fluorescence, Microscopy, Periodic Counter-current Chromatography
2) Product Images from "Disease-causing Mutation in PKR2 Receptor Reveals a Critical Role of Positive Charges in the Second Intracellular Loop for G-protein Coupling and Receptor Trafficking"
Article Title: Disease-causing Mutation in PKR2 Receptor Reveals a Critical Role of Positive Charges in the Second Intracellular Loop for G-protein Coupling and Receptor Trafficking
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.M111.223784
Figure Legend Snippet: The effect of R164Q mutation on the signal transduction and cell surface expression of PKR2. A , R164Q mutation disrupted the signal transduction of PKR2 as assayed with an aequorin-based assay. B , both WT and R164Q-PKR2 were presented on the cell surface. The Alexa Fluor 594-conjugated concanavalin A was used to label the membrane. The fluorescence was monitored with a confocal microscope. Scale bar: 10 μm.
Techniques Used: Mutagenesis, Transduction, Expressing, Fluorescence, Microscopy
3) Product Images from "Relationships between MA-RNA Binding in Cells and Suppression of HIV-1 Gag Mislocalization to Intracellular Membranes"
Article Title: Relationships between MA-RNA Binding in Cells and Suppression of HIV-1 Gag Mislocalization to Intracellular Membranes
Journal: Journal of Virology
doi: 10.1128/JVI.00756-19
Figure Legend Snippet: Gag derivatives containing KR substitutions in the MA-HBR do not show promiscuous localization in cells, unlike those with KT changes. (A and B) HeLa cells were transfected with full-length Gag-YFP (A) and delNC/Gag-YFP (B), which contain the WT MA sequence or 25/26KR, 25/26KT, 29/31KR, or 29/31KT. At 14 h posttransfection, cells were stained with Alexa Fluor 594-conjugated concanavalin A (ConA-AF594), fixed with 4% paraformaldehyde in PBS, and analyzed using a fluorescence microscope. Note that subcellular distributions of delNC/Gag-YFP constructs mirror those of the corresponding full-length Gag-YFP constructs for both WT and MA-HBR mutants. Forty-two to 85 cells were analyzed under each condition across 3 independent experiments. The localization patterns determined by epifluorescence microscopy were confirmed by confocal microscopy. (Top) Representative confocal images. The blue arrowhead indicates the Gag-YFP signal in the intracellular compartments (IC). (Bottom) Bar graphs representing percentages of cells showing the indicated patterns of subcellular distribution for each Gag-YFP construct. ConA-AF594 staining was used as a plasma membrane (PM) marker (not shown). (C, top) Images showing an example of Gag-YFP showing PM-specific localization and ConA-AF594 staining of the PM. Green arrowheads indicate overlap of the two signals at the cell surface in a merged image. (Bottom) Images showing an example of Gag-YFP exhibiting PM and IC localization. The blue arrowhead indicates the Gag-YFP signal in the intracellular compartments. (D) HeLa cells were transfected with full-length WT Gag-YFP or 29/31KA Gag-YFP. At 16 h posttransfection, cells were stained with ConA-AF594, fixed with 4% paraformaldehyde in PBS, and analyzed using a fluorescence microscope. A total of 45 to 62 cells in 3 independent experiments were analyzed. The localization patterns determined by epifluorescence microscopy were confirmed by confocal microscopy. (Top) Representative confocal images. The blue arrowhead indicates the Gag-YFP signal in the intracellular compartments. (Bottom) Bar graphs representing percentages of cell populations as described above.
Techniques Used: Transfection, Sequencing, Staining, Fluorescence, Microscopy, Construct, Epifluorescence Microscopy, Confocal Microscopy, Marker
4) Product Images from "Molecular Determinants Directing HIV-1 Gag Assembly to Virus-Containing Compartments in Primary Macrophages"
Article Title: Molecular Determinants Directing HIV-1 Gag Assembly to Virus-Containing Compartments in Primary Macrophages
Journal: Journal of Virology
doi: 10.1128/JVI.01004-16
Figure Legend Snippet: HIV-1 Gag failed to localize to VCCs upon 5ptaseIV overexpression. (A) MDMs were infected with pseudotyped HIV-1 encoding Gag-YFP along with 5ptaseIV (FL) or 5ptaseIV Δ1. At 48 h postinfection, cells were stained with ConA labeled with Alexa Fluor 594 (a PM marker), fixed, immunostained with a mouse monoclonal anti-CD81 antibody and anti-mouse IgG conjugated with Alexa Fluor 647, and analyzed using a confocal microscope. (B) Pearson's correlation coefficients for colocalization of Gag-YFP with CD81 are shown as means and standard errors of the means (SEM). Twenty to 30 cells were analyzed per condition. **, P
Techniques Used: Over Expression, Infection, Staining, Labeling, Marker, Microscopy
Figure Legend Snippet: The myristate moiety but not the intact HIV-1 MA sequence is required for VCC localization. (A) MDMs were infected with pseudotyped HIV-1 encoding WT Gag-YFP or Gag-YFP derivatives containing the indicated MA substitutions. At 48 h postinfection, cells were stained with ConA labeled with Alexa Fluor 594, fixed, immunostained with a mouse monoclonal anti-CD81 antibody and anti-mouse IgG conjugated with Alexa Fluor 647, and analyzed using a confocal microscope. (B) Pearson's correlation coefficients for colocalization of Gag-YFP with CD81 are shown as means and SEM. Twenty to 30 cells were analyzed per condition. *, P
Techniques Used: Sequencing, Infection, Staining, Labeling, Microscopy
Figure Legend Snippet: Heterologous membrane binding sequences can replace HIV-1 MA without affecting VCC localization. (A) Schematic illustrations of WT, PH/ΔMA, Kmyr/ΔMA, and Fyn(10)/ΔMA Gag-YFP. (B) MDMs were infected with pseudotyped HIV-1 encoding WT Gag-YFP or Gag-YFP derivatives in which MA was replaced by a heterologous membrane binding sequence. At 48 h postinfection, cells were stained with ConA labeled with Alexa Fluor 594, fixed, immunostained with a mouse monoclonal anti-CD81 antibody and anti-mouse IgG conjugated with Alexa Fluor 647, and analyzed using a confocal microscope. (C) Pearson's correlation coefficients for colocalization of Gag-YFP with CD81 are shown as means and SEM. Twenty to 30 cells were analyzed per condition. n.s., not significant.
Techniques Used: Binding Assay, Infection, Sequencing, Staining, Labeling, Microscopy
Figure Legend Snippet: HIV-1 Gag localization to VCCs requires higher-order multimerization. (A) Schematic illustrations of WT, EE75,76AA, delNC, LZ, and LZ4 Gag-YFP. LZ4 Gag-YFP contains the WM184,185AA CA mutation, which disrupts Gag dimerization, in addition to replacement of NC with a tetramer-forming LZ sequence. (B) MDMs were infected with pseudotyped HIV-1 encoding WT Gag-YFP or Gag-YFP derivatives containing the substitutions shown in panel A. At 48 h postinfection, cells were stained with ConA labeled with Alexa Fluor 594, fixed, immunostained with a mouse monoclonal anti-CD81 antibody and anti-mouse IgG conjugated with Alexa Fluor 647, and analyzed using a confocal microscope. Note that for cells expressing delNC Gag-YFP or LZ4 Gag-YFP, prominent YFP signals were found at the cell surface as detected by ConA (white arrowheads), whereas such surface YFP signals were nearly undetectable in WT Gag-YFP-expressing cells. (C) Pearson's correlation coefficients for colocalization of Gag-YFP with ConA are shown as means and SEM. Twenty to 40 cells were analyzed per condition. *, P
Techniques Used: Mutagenesis, Sequencing, Infection, Staining, Labeling, Microscopy, Expressing
5) Product Images from "Gag Localization and Virus-Like Particle Release Mediated by the Matrix Domain of Human T-Lymphotropic Virus Type 1 Gag Are Less Dependent on Phosphatidylinositol-(4,5)-Bisphosphate than Those Mediated by the Matrix Domain of HIV-1 Gag ▿"
Article Title: Gag Localization and Virus-Like Particle Release Mediated by the Matrix Domain of Human T-Lymphotropic Virus Type 1 Gag Are Less Dependent on Phosphatidylinositol-(4,5)-Bisphosphate than Those Mediated by the Matrix Domain of HIV-1 Gag ▿
Journal: Journal of Virology
doi: 10.1128/JVI.02383-10
Figure Legend Snippet: Localization of both HTLV-1 and HTMA Gag remains unchanged upon 5ptaseIV overexpression, unlike that of HIV-1 Gag. (a) HeLa cells expressing Gag-eCFP constructs were fixed and stained with ConA conjugated with Alexa Fluor 594. Note that HIV-1 Gag-eCFP
Techniques Used: Over Expression, Expressing, Construct, Staining
6) Product Images from "Role of Nongenomic Signaling Pathways Activated by Aldosterone During Cardiac Reperfusion Injury"
Article Title: Role of Nongenomic Signaling Pathways Activated by Aldosterone During Cardiac Reperfusion Injury
Journal: Molecular Endocrinology
doi: 10.1210/ME.2014-1410
Figure Legend Snippet: Localization of MR and GPER in LV infarcted myocardium. Representative overlay confocal microscopy images of LV sections from hearts subjected to I-R injury. MR (A) and GPR30 (B) were detected with Alexa 488 (green). Tissue sections were costained with Alexa 594 conjugate Con A (red), which specifically binds α-mannosyl saccharides in plasma membrane glycoproteins. Nuclei are stained using 4′,6′-diamino-2-phenylindole (DAPI) (blue). Scale bar, 10 μm.
Techniques Used: Confocal Microscopy, Staining
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