alexa fluor 568 conjugated goat anti human igg  (Thermo Fisher)


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    Name:
    Goat anti Human IgG H L Cross Adsorbed Secondary Antibody
    Description:
    Goat anti Human IgG H L Cross Adsorbed Secondary Antibody for Western Blot IF ICC IHC Flow IP ELISA
    Catalog Number:
    31119
    Price:
    None
    Category:
    Antibodies Secondary Detection Reagents
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    Structured Review

    Thermo Fisher alexa fluor 568 conjugated goat anti human igg
    LPS treatment increases vascular leakage. A , B Intravenously injected tracer (FITC-albumin, MW = 66 kD) accumulates throughout the entire vestibular system of LPS-treated animals ( B ), but not in controls ( A ). C Capillary leakage of FITC-albumin is significant in LPS-treated mice [ n = 6, P (control vs LPS treatment) = 0.007]. D – G Extravasation of intravenously injected <t>IgG</t> <t>Alexa</t> 568 ( white arrows ) from capillaries, visualized by collagen IV immunostaining ( green ), was observed in LPS-treated mice, but not in controls. The scale bar is 50 μm.
    Goat anti Human IgG H L Cross Adsorbed Secondary Antibody for Western Blot IF ICC IHC Flow IP ELISA
    https://www.bioz.com/result/alexa fluor 568 conjugated goat anti human igg/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 568 conjugated goat anti human igg - by Bioz Stars, 2021-06
    98/100 stars

    Images

    1) Product Images from "Characterization and Inflammatory Response of Perivascular-Resident Macrophage-Like Melanocytes in the Vestibular System"

    Article Title: Characterization and Inflammatory Response of Perivascular-Resident Macrophage-Like Melanocytes in the Vestibular System

    Journal: JARO: Journal of the Association for Research in Otolaryngology

    doi: 10.1007/s10162-013-0403-2

    LPS treatment increases vascular leakage. A , B Intravenously injected tracer (FITC-albumin, MW = 66 kD) accumulates throughout the entire vestibular system of LPS-treated animals ( B ), but not in controls ( A ). C Capillary leakage of FITC-albumin is significant in LPS-treated mice [ n = 6, P (control vs LPS treatment) = 0.007]. D – G Extravasation of intravenously injected IgG Alexa 568 ( white arrows ) from capillaries, visualized by collagen IV immunostaining ( green ), was observed in LPS-treated mice, but not in controls. The scale bar is 50 μm.
    Figure Legend Snippet: LPS treatment increases vascular leakage. A , B Intravenously injected tracer (FITC-albumin, MW = 66 kD) accumulates throughout the entire vestibular system of LPS-treated animals ( B ), but not in controls ( A ). C Capillary leakage of FITC-albumin is significant in LPS-treated mice [ n = 6, P (control vs LPS treatment) = 0.007]. D – G Extravasation of intravenously injected IgG Alexa 568 ( white arrows ) from capillaries, visualized by collagen IV immunostaining ( green ), was observed in LPS-treated mice, but not in controls. The scale bar is 50 μm.

    Techniques Used: Injection, Mouse Assay, Immunostaining

    2) Product Images from "Endocytosis of the receptor-binding domain of SARS-CoV spike protein together with virus receptor ACE2"

    Article Title: Endocytosis of the receptor-binding domain of SARS-CoV spike protein together with virus receptor ACE2

    Journal: Virus Research

    doi: 10.1016/j.virusres.2008.03.004

    SARS-CoV RBD spike protein was internalized by the susceptible cells together with ACE2. (A) Decreased cell surface-bound RBD-Fc protein following incubation with VeroE6 cells at 37 °C compared to 4 °C. After incubation with VeroE6 cells for 3 h, cell surface-bound Fc or RBD-Fc proteins were detected using a FITC-conjugated human IgG-specific antibody. Representative FACS histograms are shown for three independent experiments. Blue and black histograms represent the results from the cells incubated with the RBD-Fc or Fc at 4 °C, respectively. The red histogram represents the results from the cells incubated with the RBD-Fc at 37 °C. (B) ACE2 internalization is the RBD spike protein-dependent. After ACE2-GFP-293 cells were incubated with the RBD-Fc or Fc protein at 37 °C for 3 h, the distribution of ACE2-GFP molecules in live cells was observed under a fluorescence microscope. (C) Co-localization of ACE2 and the RBD of SARS-CoV in ACE2-GFP-293 cells under a confocal laser-scanning microscope. After incubation with the RBD-Fc or Fc protein at 37 °C for 3 h, ACE2-GFP-293 cells were washed to remove excess RBD-Fc or Fc protein and then fixed with 4% paraformaldehyde. Immunostaining was performed using an Alexa Fluor 568-conjugated human IgG-specific antibody to detect the distribution of the Fc or RBD-Fc protein in the cells. (D) and (E) Statistical analysis of the differences between the RBD-Fc and Fc protein treatments (Chi-square test). ** P
    Figure Legend Snippet: SARS-CoV RBD spike protein was internalized by the susceptible cells together with ACE2. (A) Decreased cell surface-bound RBD-Fc protein following incubation with VeroE6 cells at 37 °C compared to 4 °C. After incubation with VeroE6 cells for 3 h, cell surface-bound Fc or RBD-Fc proteins were detected using a FITC-conjugated human IgG-specific antibody. Representative FACS histograms are shown for three independent experiments. Blue and black histograms represent the results from the cells incubated with the RBD-Fc or Fc at 4 °C, respectively. The red histogram represents the results from the cells incubated with the RBD-Fc at 37 °C. (B) ACE2 internalization is the RBD spike protein-dependent. After ACE2-GFP-293 cells were incubated with the RBD-Fc or Fc protein at 37 °C for 3 h, the distribution of ACE2-GFP molecules in live cells was observed under a fluorescence microscope. (C) Co-localization of ACE2 and the RBD of SARS-CoV in ACE2-GFP-293 cells under a confocal laser-scanning microscope. After incubation with the RBD-Fc or Fc protein at 37 °C for 3 h, ACE2-GFP-293 cells were washed to remove excess RBD-Fc or Fc protein and then fixed with 4% paraformaldehyde. Immunostaining was performed using an Alexa Fluor 568-conjugated human IgG-specific antibody to detect the distribution of the Fc or RBD-Fc protein in the cells. (D) and (E) Statistical analysis of the differences between the RBD-Fc and Fc protein treatments (Chi-square test). ** P

    Techniques Used: Incubation, FACS, Fluorescence, Microscopy, Laser-Scanning Microscopy, Immunostaining

    Related Articles

    In Situ:

    Article Title: Characterization and Inflammatory Response of Perivascular-Resident Macrophage-Like Melanocytes in the Vestibular System
    Article Snippet: Relative fluorescence of the supernatant was measured on a Tecan GENios Plus Microplate Reader (Tecan Group Ltd, USA), with samples for each group run in quintuplicate. .. For in situ detection, Alexa Fluor 568-conjugated goat anti-human IgG (molecular mass, 200 kDa) (A-21090, Invitrogen, USA) was administered by i.v. for 2 h. Anesthetized animals were perfused intravascularly through the left ventricle for 2 min with HBSS followed by 5 min of 4 % paraformaldehyde (PFA) in PBS. ..

    Immunofluorescence:

    Article Title: Identification of geraldol as an inhibitor of aquaporin-4 binding by NMO-IgG
    Article Snippet: Luminescence was recorded after 5 min using a microplate reader (Fluostar Optima; BMG Lab Tech GmbH). .. Immunofluorescence stainingV79 cells expressing M23-AQP4 were cultured on round glass coverslips in 24-well plate (Corning, Inc.) for 24 h. After washing three times with PBS, the cells were blocked with 3% BSA (Merck KGaA) at room temperature for 1 h. Geraldol or DMSO was added in 3% BSA and incubated at room temperature for 15 min, followed by the addition of NMO-IgG (20 µg/ml) or control serum from patients without NMO (1:200) at room temperature for 1 h. V79-AQP4 cells were washed with PBS three times and incubated with Alexa Fluor 555-conjugated goat anti-human IgG secondary antibody (1:400; Invitrogen; Thermo Fisher Scientific, Inc; cat. no. A-21433). .. For AQP4 immunofluorescence staining, V79-AQP4 cells were fixed in 4% PFA at room temperature for 10 min and permeabilized with 0.5% Triton X-100 at room temperature for 10 min. After blocking with 3% BSA at room temperature for 1 h, the cells were incubated with rabbit anti-AQP4 antibody (1:200; Santa Cruz Biotechnology; cat. no. sc-32739) at room temperature for 1 h. Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200; Invitrogen; Thermo Fisher Scientific, Inc.; cat. no. A32731) was used as the secondary antibody.

    Article Title: Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus
    Article Snippet: HCV Infectivity Assay The level of infectivity of the HCV produced in cell culture was measured following the TCID50 protocol outlined by Lindenbach [ ]. .. A human HCV antiserum at a 1/500 dilution (initially validated against the anti-HCV capsid C7/50 clone (Abcam) in a double immunofluorescence assay) and a goat anti-human Alexa 488 secondary antibody (Invitrogen) were used, at a concentration of 1 μg/mL. .. Cells were counterstained with DAPI and examined with a Nikon TE-2000E epifluorescence microscope.

    Expressing:

    Article Title: Identification of geraldol as an inhibitor of aquaporin-4 binding by NMO-IgG
    Article Snippet: Luminescence was recorded after 5 min using a microplate reader (Fluostar Optima; BMG Lab Tech GmbH). .. Immunofluorescence stainingV79 cells expressing M23-AQP4 were cultured on round glass coverslips in 24-well plate (Corning, Inc.) for 24 h. After washing three times with PBS, the cells were blocked with 3% BSA (Merck KGaA) at room temperature for 1 h. Geraldol or DMSO was added in 3% BSA and incubated at room temperature for 15 min, followed by the addition of NMO-IgG (20 µg/ml) or control serum from patients without NMO (1:200) at room temperature for 1 h. V79-AQP4 cells were washed with PBS three times and incubated with Alexa Fluor 555-conjugated goat anti-human IgG secondary antibody (1:400; Invitrogen; Thermo Fisher Scientific, Inc; cat. no. A-21433). .. For AQP4 immunofluorescence staining, V79-AQP4 cells were fixed in 4% PFA at room temperature for 10 min and permeabilized with 0.5% Triton X-100 at room temperature for 10 min. After blocking with 3% BSA at room temperature for 1 h, the cells were incubated with rabbit anti-AQP4 antibody (1:200; Santa Cruz Biotechnology; cat. no. sc-32739) at room temperature for 1 h. Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200; Invitrogen; Thermo Fisher Scientific, Inc.; cat. no. A32731) was used as the secondary antibody.

    Cell Culture:

    Article Title: Identification of geraldol as an inhibitor of aquaporin-4 binding by NMO-IgG
    Article Snippet: Luminescence was recorded after 5 min using a microplate reader (Fluostar Optima; BMG Lab Tech GmbH). .. Immunofluorescence stainingV79 cells expressing M23-AQP4 were cultured on round glass coverslips in 24-well plate (Corning, Inc.) for 24 h. After washing three times with PBS, the cells were blocked with 3% BSA (Merck KGaA) at room temperature for 1 h. Geraldol or DMSO was added in 3% BSA and incubated at room temperature for 15 min, followed by the addition of NMO-IgG (20 µg/ml) or control serum from patients without NMO (1:200) at room temperature for 1 h. V79-AQP4 cells were washed with PBS three times and incubated with Alexa Fluor 555-conjugated goat anti-human IgG secondary antibody (1:400; Invitrogen; Thermo Fisher Scientific, Inc; cat. no. A-21433). .. For AQP4 immunofluorescence staining, V79-AQP4 cells were fixed in 4% PFA at room temperature for 10 min and permeabilized with 0.5% Triton X-100 at room temperature for 10 min. After blocking with 3% BSA at room temperature for 1 h, the cells were incubated with rabbit anti-AQP4 antibody (1:200; Santa Cruz Biotechnology; cat. no. sc-32739) at room temperature for 1 h. Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200; Invitrogen; Thermo Fisher Scientific, Inc.; cat. no. A32731) was used as the secondary antibody.

    Incubation:

    Article Title: Identification of geraldol as an inhibitor of aquaporin-4 binding by NMO-IgG
    Article Snippet: Luminescence was recorded after 5 min using a microplate reader (Fluostar Optima; BMG Lab Tech GmbH). .. Immunofluorescence stainingV79 cells expressing M23-AQP4 were cultured on round glass coverslips in 24-well plate (Corning, Inc.) for 24 h. After washing three times with PBS, the cells were blocked with 3% BSA (Merck KGaA) at room temperature for 1 h. Geraldol or DMSO was added in 3% BSA and incubated at room temperature for 15 min, followed by the addition of NMO-IgG (20 µg/ml) or control serum from patients without NMO (1:200) at room temperature for 1 h. V79-AQP4 cells were washed with PBS three times and incubated with Alexa Fluor 555-conjugated goat anti-human IgG secondary antibody (1:400; Invitrogen; Thermo Fisher Scientific, Inc; cat. no. A-21433). .. For AQP4 immunofluorescence staining, V79-AQP4 cells were fixed in 4% PFA at room temperature for 10 min and permeabilized with 0.5% Triton X-100 at room temperature for 10 min. After blocking with 3% BSA at room temperature for 1 h, the cells were incubated with rabbit anti-AQP4 antibody (1:200; Santa Cruz Biotechnology; cat. no. sc-32739) at room temperature for 1 h. Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:200; Invitrogen; Thermo Fisher Scientific, Inc.; cat. no. A32731) was used as the secondary antibody.

    Article Title: Targeting Vesicular LGALS3BP by an Antibody-Drug Conjugate as Novel Therapeutic Strategy for Neuroblastoma
    Article Snippet: Animals received a single injection of vehicle (PBS) or 1959-sss/DM3 intravenously and were sacrificed 72 h later. .. Fresh tumor tissues were frozen in a crio-embedding medium (OCT, BioOptica, Milan, Italy) and cryostat sections were incubated with the following antibodies: AlexaFluor-488 conjugated anti-human IgG 1:200 (A11013, Invitrogen, Life Technologies) in order to detect 1959-sss/DM3 and rat monoclonal anti-CD31 (550274, BD Pharmingen, Franklin Lakes, NJ, USA), mixed with rat monoclonal anti-CD105 (550546, BD Pharmingen) at the diluition of 1:40, followed by secondary antibody AlexaFluor-546 conjugated 1:200 (A11081, Molecular Probes, Life Technologies). .. Endothelial cells were identified by immunohistochemical labeling of tissue sections using a platelet-endothelial cell adhesion molecule (PECAM-1/CD31) and endoglin (CD105).

    Article Title: Generation of a Neutralizing Human Monoclonal Antibody Fab Fragment to Surface Antigen 1 of Toxoplasma gondii Tachyzoites ▿
    Article Snippet: The slides were treated with 0.05% Triton X-100 in PBS for 5 min. After being washed with PBS, the samples were blocked with 3% bovine serum albumin in PBS for 30 min and then incubated with Fab fragments for 1 h at room temperature. .. After being washed again with PBS, the cells were incubated with Alexa Fluor 488-labeled goat anti-human IgG antibody (Molecular Probes, Eugene, OR) for 1 h. After final washing, the stained trophozoites were mounted using glycerol containing 1.25 μg of DAPI/ml, 1.25 mg of 1,4-diazabicyclo(2,2,2)octane/ml, and 10% PBS. .. Samples were observed by using a Zeiss LSM700 confocal laser scanning microscope.

    Concentration Assay:

    Article Title: Epidermal Growth Factor Receptor-Dependent Mutual Amplification between Netrin-1 and the Hepatitis C Virus
    Article Snippet: HCV Infectivity Assay The level of infectivity of the HCV produced in cell culture was measured following the TCID50 protocol outlined by Lindenbach [ ]. .. A human HCV antiserum at a 1/500 dilution (initially validated against the anti-HCV capsid C7/50 clone (Abcam) in a double immunofluorescence assay) and a goat anti-human Alexa 488 secondary antibody (Invitrogen) were used, at a concentration of 1 μg/mL. .. Cells were counterstained with DAPI and examined with a Nikon TE-2000E epifluorescence microscope.

    Staining:

    Article Title: Generation of a Neutralizing Human Monoclonal Antibody Fab Fragment to Surface Antigen 1 of Toxoplasma gondii Tachyzoites ▿
    Article Snippet: The slides were treated with 0.05% Triton X-100 in PBS for 5 min. After being washed with PBS, the samples were blocked with 3% bovine serum albumin in PBS for 30 min and then incubated with Fab fragments for 1 h at room temperature. .. After being washed again with PBS, the cells were incubated with Alexa Fluor 488-labeled goat anti-human IgG antibody (Molecular Probes, Eugene, OR) for 1 h. After final washing, the stained trophozoites were mounted using glycerol containing 1.25 μg of DAPI/ml, 1.25 mg of 1,4-diazabicyclo(2,2,2)octane/ml, and 10% PBS. .. Samples were observed by using a Zeiss LSM700 confocal laser scanning microscope.

    other:

    Article Title: Regulation of Varicella-Zoster Virus-Induced Cell-to-Cell Fusion by the Endocytosis-Competent Glycoproteins gH and gE
    Article Snippet: Fluoroconjugated goat anti-human Alexa 488, goat anti-mouse Texas Red-X, and goat anti-mouse Alexa 488 were the secondary reagents for confocal analysis of VZV glycoproteins (Molecular Probes, Eugene, Oreg.).

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  • 98
    Thermo Fisher alexa fluor 568 conjugated goat anti human igg
    LPS treatment increases vascular leakage. A , B Intravenously injected tracer (FITC-albumin, MW = 66 kD) accumulates throughout the entire vestibular system of LPS-treated animals ( B ), but not in controls ( A ). C Capillary leakage of FITC-albumin is significant in LPS-treated mice [ n = 6, P (control vs LPS treatment) = 0.007]. D – G Extravasation of intravenously injected <t>IgG</t> <t>Alexa</t> 568 ( white arrows ) from capillaries, visualized by collagen IV immunostaining ( green ), was observed in LPS-treated mice, but not in controls. The scale bar is 50 μm.
    Alexa Fluor 568 Conjugated Goat Anti Human Igg, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa fluor 568 conjugated goat anti human igg/product/Thermo Fisher
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa fluor 568 conjugated goat anti human igg - by Bioz Stars, 2021-06
    98/100 stars
      Buy from Supplier

    97
    Thermo Fisher goat anti mouse antibody conjugated to alexa fluor 568
    LPS treatment increases vascular leakage. A , B Intravenously injected tracer (FITC-albumin, MW = 66 kD) accumulates throughout the entire vestibular system of LPS-treated animals ( B ), but not in controls ( A ). C Capillary leakage of FITC-albumin is significant in LPS-treated mice [ n = 6, P (control vs LPS treatment) = 0.007]. D – G Extravasation of intravenously injected <t>IgG</t> <t>Alexa</t> 568 ( white arrows ) from capillaries, visualized by collagen IV immunostaining ( green ), was observed in LPS-treated mice, but not in controls. The scale bar is 50 μm.
    Goat Anti Mouse Antibody Conjugated To Alexa Fluor 568, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse antibody conjugated to alexa fluor 568/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse antibody conjugated to alexa fluor 568 - by Bioz Stars, 2021-06
    97/100 stars
      Buy from Supplier

    Image Search Results


    LPS treatment increases vascular leakage. A , B Intravenously injected tracer (FITC-albumin, MW = 66 kD) accumulates throughout the entire vestibular system of LPS-treated animals ( B ), but not in controls ( A ). C Capillary leakage of FITC-albumin is significant in LPS-treated mice [ n = 6, P (control vs LPS treatment) = 0.007]. D – G Extravasation of intravenously injected IgG Alexa 568 ( white arrows ) from capillaries, visualized by collagen IV immunostaining ( green ), was observed in LPS-treated mice, but not in controls. The scale bar is 50 μm.

    Journal: JARO: Journal of the Association for Research in Otolaryngology

    Article Title: Characterization and Inflammatory Response of Perivascular-Resident Macrophage-Like Melanocytes in the Vestibular System

    doi: 10.1007/s10162-013-0403-2

    Figure Lengend Snippet: LPS treatment increases vascular leakage. A , B Intravenously injected tracer (FITC-albumin, MW = 66 kD) accumulates throughout the entire vestibular system of LPS-treated animals ( B ), but not in controls ( A ). C Capillary leakage of FITC-albumin is significant in LPS-treated mice [ n = 6, P (control vs LPS treatment) = 0.007]. D – G Extravasation of intravenously injected IgG Alexa 568 ( white arrows ) from capillaries, visualized by collagen IV immunostaining ( green ), was observed in LPS-treated mice, but not in controls. The scale bar is 50 μm.

    Article Snippet: For in situ detection, Alexa Fluor 568-conjugated goat anti-human IgG (molecular mass, 200 kDa) (A-21090, Invitrogen, USA) was administered by i.v. for 2 h. Anesthetized animals were perfused intravascularly through the left ventricle for 2 min with HBSS followed by 5 min of 4 % paraformaldehyde (PFA) in PBS.

    Techniques: Injection, Mouse Assay, Immunostaining

    SARS-CoV RBD spike protein was internalized by the susceptible cells together with ACE2. (A) Decreased cell surface-bound RBD-Fc protein following incubation with VeroE6 cells at 37 °C compared to 4 °C. After incubation with VeroE6 cells for 3 h, cell surface-bound Fc or RBD-Fc proteins were detected using a FITC-conjugated human IgG-specific antibody. Representative FACS histograms are shown for three independent experiments. Blue and black histograms represent the results from the cells incubated with the RBD-Fc or Fc at 4 °C, respectively. The red histogram represents the results from the cells incubated with the RBD-Fc at 37 °C. (B) ACE2 internalization is the RBD spike protein-dependent. After ACE2-GFP-293 cells were incubated with the RBD-Fc or Fc protein at 37 °C for 3 h, the distribution of ACE2-GFP molecules in live cells was observed under a fluorescence microscope. (C) Co-localization of ACE2 and the RBD of SARS-CoV in ACE2-GFP-293 cells under a confocal laser-scanning microscope. After incubation with the RBD-Fc or Fc protein at 37 °C for 3 h, ACE2-GFP-293 cells were washed to remove excess RBD-Fc or Fc protein and then fixed with 4% paraformaldehyde. Immunostaining was performed using an Alexa Fluor 568-conjugated human IgG-specific antibody to detect the distribution of the Fc or RBD-Fc protein in the cells. (D) and (E) Statistical analysis of the differences between the RBD-Fc and Fc protein treatments (Chi-square test). ** P

    Journal: Virus Research

    Article Title: Endocytosis of the receptor-binding domain of SARS-CoV spike protein together with virus receptor ACE2

    doi: 10.1016/j.virusres.2008.03.004

    Figure Lengend Snippet: SARS-CoV RBD spike protein was internalized by the susceptible cells together with ACE2. (A) Decreased cell surface-bound RBD-Fc protein following incubation with VeroE6 cells at 37 °C compared to 4 °C. After incubation with VeroE6 cells for 3 h, cell surface-bound Fc or RBD-Fc proteins were detected using a FITC-conjugated human IgG-specific antibody. Representative FACS histograms are shown for three independent experiments. Blue and black histograms represent the results from the cells incubated with the RBD-Fc or Fc at 4 °C, respectively. The red histogram represents the results from the cells incubated with the RBD-Fc at 37 °C. (B) ACE2 internalization is the RBD spike protein-dependent. After ACE2-GFP-293 cells were incubated with the RBD-Fc or Fc protein at 37 °C for 3 h, the distribution of ACE2-GFP molecules in live cells was observed under a fluorescence microscope. (C) Co-localization of ACE2 and the RBD of SARS-CoV in ACE2-GFP-293 cells under a confocal laser-scanning microscope. After incubation with the RBD-Fc or Fc protein at 37 °C for 3 h, ACE2-GFP-293 cells were washed to remove excess RBD-Fc or Fc protein and then fixed with 4% paraformaldehyde. Immunostaining was performed using an Alexa Fluor 568-conjugated human IgG-specific antibody to detect the distribution of the Fc or RBD-Fc protein in the cells. (D) and (E) Statistical analysis of the differences between the RBD-Fc and Fc protein treatments (Chi-square test). ** P

    Article Snippet: After incubation with Fc or RBD-Fc as described above, the cells were washed with phosphate-buffered saline (PBS) three times, and then fixed with 4% paraformaldehyde for 15 min and permearized with 0.1% TritonX-100/PBS for 30 min. After being blocked with 1% BSA in 1× PBS, samples were incubated with Alexa Fluor 568-conjugated goat anti-human IgG (H + L) (1:200, Molecular Probes) at room temperature for 1 h and washed with 0.5% Tween20/PBS three times.

    Techniques: Incubation, FACS, Fluorescence, Microscopy, Laser-Scanning Microscopy, Immunostaining