alexa 647 dye  (New England Biolabs)


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  • 95
    Name:
    SNAP Surface Alexa Fluor 647
    Description:

    Catalog Number:
    S9136
    Price:
    392
    Category:
    Biochemicals
    Applications:
    Cellular Biology
    Size:
    50 nmol
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    Structured Review

    New England Biolabs alexa 647 dye
    SNAP Surface Alexa Fluor 647

    https://www.bioz.com/result/alexa 647 dye/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa 647 dye - by Bioz Stars, 2021-09
    95/100 stars

    Images

    1) Product Images from "A structural model for microtubule minus-end recognition and protection by CAMSAP proteins"

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins

    Journal: Nature structural & molecular biology

    doi: 10.1038/nsmb.3483

    CAMSAP CKKs protect MT minus ends from MCAK-induced depolymerization via steric inhibition. ( a ) A MT tubulin dimer-pair bound to CAMSAP3-CKK (green) is shown with the expected position of an MD of human MCAK (in complex with ADP; PDB ID 4UBF) by alignment with MT-bound kinesin-1 47  in Chimera 48 . b ) Kymographs of MT depolymerization assay with GMPCPP-stabilized MTs (blue) and GFP-MCAK (red). MT polarity was determined based on the movement of the SNAP-Alexa647-tagged plus-end directed motor kinesin-1 KIF5B (green, residues 1–560). Scale bars: horizontal, 1 μm; vertical, upper panel, 1 sec, lower panels, 1 min. c ) Kymographs of MT depolymerization assays with GMPCPP-stabilized MTs (blue), GFP-MCAK (red) and different concentrations of SNAP-Alexa 647 CAMSAP1 mini  or CKK (green). Scale bars: horizontal, 1 μm; vertical, 1 min. In panels (b) and (c), MT minus (−) ends are shown on the left and plus (+) ends on the right. d-e ) Quantification of MT depolymerization rate, MCAK intensity and CAMSAP1 intensity at different concentrations of CAMSAP1 mini  (d) or CKK (e). n ranged from 17 to 31 MTs; individual data points are provided in  Supplementary Table 2 . Data represent mean ± SD. See also  Supplementary Table 2 .
    Figure Legend Snippet: CAMSAP CKKs protect MT minus ends from MCAK-induced depolymerization via steric inhibition. ( a ) A MT tubulin dimer-pair bound to CAMSAP3-CKK (green) is shown with the expected position of an MD of human MCAK (in complex with ADP; PDB ID 4UBF) by alignment with MT-bound kinesin-1 47 in Chimera 48 . b ) Kymographs of MT depolymerization assay with GMPCPP-stabilized MTs (blue) and GFP-MCAK (red). MT polarity was determined based on the movement of the SNAP-Alexa647-tagged plus-end directed motor kinesin-1 KIF5B (green, residues 1–560). Scale bars: horizontal, 1 μm; vertical, upper panel, 1 sec, lower panels, 1 min. c ) Kymographs of MT depolymerization assays with GMPCPP-stabilized MTs (blue), GFP-MCAK (red) and different concentrations of SNAP-Alexa 647 CAMSAP1 mini or CKK (green). Scale bars: horizontal, 1 μm; vertical, 1 min. In panels (b) and (c), MT minus (−) ends are shown on the left and plus (+) ends on the right. d-e ) Quantification of MT depolymerization rate, MCAK intensity and CAMSAP1 intensity at different concentrations of CAMSAP1 mini (d) or CKK (e). n ranged from 17 to 31 MTs; individual data points are provided in Supplementary Table 2 . Data represent mean ± SD. See also Supplementary Table 2 .

    Techniques Used: Inhibition, Size-exclusion Chromatography

    Related Articles

    Blocking Assay:

    Article Title: 3D particle averaging and detection of macromolecular symmetry in localization microscopy
    Article Snippet: .. The benzylguanine (BG)-conjugated AF647 (SNAP-Surface; NEB; cat.# S9136S) was diluted to 1 μM in blocking solution (0,5% (w/v) BSA, 1 mM DTT in 1x PBS) and incubated with the sample for 1 hour. ..

    Incubation:

    Article Title: 3D particle averaging and detection of macromolecular symmetry in localization microscopy
    Article Snippet: .. The benzylguanine (BG)-conjugated AF647 (SNAP-Surface; NEB; cat.# S9136S) was diluted to 1 μM in blocking solution (0,5% (w/v) BSA, 1 mM DTT in 1x PBS) and incubated with the sample for 1 hour. ..

    Article Title: Residue 6.43 defines receptor function in class F GPCRs
    Article Snippet: .. 24 h later the cells were washed once with HBSS (HyClone) and incubated with 50 µl of 1 µM SNAP-surface Alexa Fluor 647 (New England Biolabs, #S9136S) in a complete DMEM medium for 30 min at 37 °C. ..

    Article Title: Exploring cell surface-nanopillar interactions with 3D super-resolution microscopy
    Article Snippet: .. The cells were incubated in labeling solution, 5% CO2 -balanced complete cell culture medium with 5 μM SNAP-Surface Alexa Fluor 647 (NEB, S9136S), for 15 minutes at 37°C. ..

    Article Title: 3D particle averaging and detection of macromolecular symmetry in localization microscopy
    Article Snippet: .. Then, the sample was incubated for 30 min with Image-iT FX Signal Enhancer (catalog no. I36933, Thermo Fisher Scientific) and then stained with SNAP dye buffer (3 μM BG-AF647 (catalog no. S9136S, New England Biolabs) and 3 μM dithiothreitol in 0.5% (w/v) bovine serum albumin (BSA) in PBS) for 2 h at room temperature. ..

    Article Title: Maximum-likelihood model fitting for quantitative analysis of SMLM data
    Article Snippet: .. Subsequently, the sample was incubated for 30 min with Image-iT FX Signal Enhancer (catalog no. I36933, Thermo Fisher Scientific) before staining with SNAP dye buffer (1 μM BG-AF647 (catalog no. S9136S, New England Biolabs) and 1 μM dithiothreitol in 0.5% [w/v] bovine serum albumin (BSA) in PBS) for 2 h at room temperature. ..

    Article Title: Residue 6.43 defines receptor function in class F GPCRs
    Article Snippet: .. Cells were washed once with HBSS and incubated with 50 µl of 1 µM SNAP-surface Alexa Fluor 647 (New England Biolabs, #S9136S) in complete DMEM medium for 30 min at 37 °C and 5% CO2. ..

    Labeling:

    Article Title: Exploring cell surface-nanopillar interactions with 3D super-resolution microscopy
    Article Snippet: .. The cells were incubated in labeling solution, 5% CO2 -balanced complete cell culture medium with 5 μM SNAP-Surface Alexa Fluor 647 (NEB, S9136S), for 15 minutes at 37°C. ..

    Article Title: Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation
    Article Snippet: .. Chemical labeling of proteins with fluorescent dyesLabeling of SNAP-tagged recombinantly expressed proteins (6His-SNAP-Toca, 6His-SNAP-VASP, 6His-SNAP-Ena, 6His-SNAP–N-WASP/ZZ-WIP, and 6His-SNAP-IRSp53) was performed with 5–10 µM final protein concentration and 10 µM dye (SNAP-Surface Alexa Fluor488 [New England Biolabs; S9129S] or SNAP-Surface Alexa Fluor647 [New England Biolabs; S9136S], respectively) in 50 µl final volume with a buffer containing 150 mM NaCl, 20 mM Hepes, pH 7.4, 1 mM DTT, and 1% TWEEN 20. ..

    Cell Culture:

    Article Title: Exploring cell surface-nanopillar interactions with 3D super-resolution microscopy
    Article Snippet: .. The cells were incubated in labeling solution, 5% CO2 -balanced complete cell culture medium with 5 μM SNAP-Surface Alexa Fluor 647 (NEB, S9136S), for 15 minutes at 37°C. ..

    Staining:

    Article Title: 3D particle averaging and detection of macromolecular symmetry in localization microscopy
    Article Snippet: .. Then, the sample was incubated for 30 min with Image-iT FX Signal Enhancer (catalog no. I36933, Thermo Fisher Scientific) and then stained with SNAP dye buffer (3 μM BG-AF647 (catalog no. S9136S, New England Biolabs) and 3 μM dithiothreitol in 0.5% (w/v) bovine serum albumin (BSA) in PBS) for 2 h at room temperature. ..

    Article Title: Maximum-likelihood model fitting for quantitative analysis of SMLM data
    Article Snippet: .. Subsequently, the sample was incubated for 30 min with Image-iT FX Signal Enhancer (catalog no. I36933, Thermo Fisher Scientific) before staining with SNAP dye buffer (1 μM BG-AF647 (catalog no. S9136S, New England Biolabs) and 1 μM dithiothreitol in 0.5% [w/v] bovine serum albumin (BSA) in PBS) for 2 h at room temperature. ..

    Protein Concentration:

    Article Title: Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation
    Article Snippet: .. Chemical labeling of proteins with fluorescent dyesLabeling of SNAP-tagged recombinantly expressed proteins (6His-SNAP-Toca, 6His-SNAP-VASP, 6His-SNAP-Ena, 6His-SNAP–N-WASP/ZZ-WIP, and 6His-SNAP-IRSp53) was performed with 5–10 µM final protein concentration and 10 µM dye (SNAP-Surface Alexa Fluor488 [New England Biolabs; S9129S] or SNAP-Surface Alexa Fluor647 [New England Biolabs; S9136S], respectively) in 50 µl final volume with a buffer containing 150 mM NaCl, 20 mM Hepes, pH 7.4, 1 mM DTT, and 1% TWEEN 20. ..

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    New England Biolabs alexa 647 dye
    CAMSAP CKKs protect MT minus ends from MCAK-induced depolymerization via steric inhibition. ( a ) A MT tubulin dimer-pair bound to CAMSAP3-CKK (green) is shown with the expected position of an MD of human MCAK (in complex with ADP; PDB ID 4UBF) by alignment with MT-bound kinesin-1 47  in Chimera 48 . b ) Kymographs of MT depolymerization assay with GMPCPP-stabilized MTs (blue) and GFP-MCAK (red). MT polarity was determined based on the movement of the SNAP-Alexa647-tagged plus-end directed motor kinesin-1 KIF5B (green, residues 1–560). Scale bars: horizontal, 1 μm; vertical, upper panel, 1 sec, lower panels, 1 min. c ) Kymographs of MT depolymerization assays with GMPCPP-stabilized MTs (blue), GFP-MCAK (red) and different concentrations of SNAP-Alexa 647 CAMSAP1 mini  or CKK (green). Scale bars: horizontal, 1 μm; vertical, 1 min. In panels (b) and (c), MT minus (−) ends are shown on the left and plus (+) ends on the right. d-e ) Quantification of MT depolymerization rate, MCAK intensity and CAMSAP1 intensity at different concentrations of CAMSAP1 mini  (d) or CKK (e). n ranged from 17 to 31 MTs; individual data points are provided in  Supplementary Table 2 . Data represent mean ± SD. See also  Supplementary Table 2 .
    Alexa 647 Dye, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alexa 647 dye/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    alexa 647 dye - by Bioz Stars, 2021-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    CAMSAP CKKs protect MT minus ends from MCAK-induced depolymerization via steric inhibition. ( a ) A MT tubulin dimer-pair bound to CAMSAP3-CKK (green) is shown with the expected position of an MD of human MCAK (in complex with ADP; PDB ID 4UBF) by alignment with MT-bound kinesin-1 47  in Chimera 48 . b ) Kymographs of MT depolymerization assay with GMPCPP-stabilized MTs (blue) and GFP-MCAK (red). MT polarity was determined based on the movement of the SNAP-Alexa647-tagged plus-end directed motor kinesin-1 KIF5B (green, residues 1–560). Scale bars: horizontal, 1 μm; vertical, upper panel, 1 sec, lower panels, 1 min. c ) Kymographs of MT depolymerization assays with GMPCPP-stabilized MTs (blue), GFP-MCAK (red) and different concentrations of SNAP-Alexa 647 CAMSAP1 mini  or CKK (green). Scale bars: horizontal, 1 μm; vertical, 1 min. In panels (b) and (c), MT minus (−) ends are shown on the left and plus (+) ends on the right. d-e ) Quantification of MT depolymerization rate, MCAK intensity and CAMSAP1 intensity at different concentrations of CAMSAP1 mini  (d) or CKK (e). n ranged from 17 to 31 MTs; individual data points are provided in  Supplementary Table 2 . Data represent mean ± SD. See also  Supplementary Table 2 .

    Journal: Nature structural & molecular biology

    Article Title: A structural model for microtubule minus-end recognition and protection by CAMSAP proteins

    doi: 10.1038/nsmb.3483

    Figure Lengend Snippet: CAMSAP CKKs protect MT minus ends from MCAK-induced depolymerization via steric inhibition. ( a ) A MT tubulin dimer-pair bound to CAMSAP3-CKK (green) is shown with the expected position of an MD of human MCAK (in complex with ADP; PDB ID 4UBF) by alignment with MT-bound kinesin-1 47 in Chimera 48 . b ) Kymographs of MT depolymerization assay with GMPCPP-stabilized MTs (blue) and GFP-MCAK (red). MT polarity was determined based on the movement of the SNAP-Alexa647-tagged plus-end directed motor kinesin-1 KIF5B (green, residues 1–560). Scale bars: horizontal, 1 μm; vertical, upper panel, 1 sec, lower panels, 1 min. c ) Kymographs of MT depolymerization assays with GMPCPP-stabilized MTs (blue), GFP-MCAK (red) and different concentrations of SNAP-Alexa 647 CAMSAP1 mini or CKK (green). Scale bars: horizontal, 1 μm; vertical, 1 min. In panels (b) and (c), MT minus (−) ends are shown on the left and plus (+) ends on the right. d-e ) Quantification of MT depolymerization rate, MCAK intensity and CAMSAP1 intensity at different concentrations of CAMSAP1 mini (d) or CKK (e). n ranged from 17 to 31 MTs; individual data points are provided in Supplementary Table 2 . Data represent mean ± SD. See also Supplementary Table 2 .

    Article Snippet: To label SNAP tagged proteins with Alexa-647 dye (NEB), 20–40 μM dye was incubated with proteins on beads for 1 hr between the washing and elution steps.

    Techniques: Inhibition, Size-exclusion Chromatography

    αSNAP dimers are the minimal units that connect NSF and SNARE complexes. a Schematic for stoichiometry determination of αSNAPs bound to surface-immobilized SNARE-incorporating vesicles. b Representative fluorescence intensity traces for the single spots in the image with the corresponding number of photobleaching step(s). c Representative fluorescence images of Alexa647-labeled αSNAP and DY549-labeled NSF from a total internal reflection fluorescence (TIRF) microscope and the corresponding counts of proteins and co-localized portions. d Deconvoluted histogram of the photobleaching step(s) for labeled αSNAP in single spots examining the number of αSNAP for NSF binding. The histogram of photobleaching step(s) in d was deconvoluted accounting for the 90% labeling efficiency. Error bars in ( c , d ) represent mean ± s.d. for n = 9 images from two independent experiments ( c ) and n = 12 images from three independent experiments ( d ). The reproducibility of the images ( b , c ) was confirmed in three independent experiments. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Extreme parsimony in ATP consumption by 20S complexes in the global disassembly of single SNARE complexes

    doi: 10.1038/s41467-021-23530-0

    Figure Lengend Snippet: αSNAP dimers are the minimal units that connect NSF and SNARE complexes. a Schematic for stoichiometry determination of αSNAPs bound to surface-immobilized SNARE-incorporating vesicles. b Representative fluorescence intensity traces for the single spots in the image with the corresponding number of photobleaching step(s). c Representative fluorescence images of Alexa647-labeled αSNAP and DY549-labeled NSF from a total internal reflection fluorescence (TIRF) microscope and the corresponding counts of proteins and co-localized portions. d Deconvoluted histogram of the photobleaching step(s) for labeled αSNAP in single spots examining the number of αSNAP for NSF binding. The histogram of photobleaching step(s) in d was deconvoluted accounting for the 90% labeling efficiency. Error bars in ( c , d ) represent mean ± s.d. for n = 9 images from two independent experiments ( c ) and n = 12 images from three independent experiments ( d ). The reproducibility of the images ( b , c ) was confirmed in three independent experiments. Source data are provided as a Source Data file.

    Article Snippet: Purified SNAP-tag-αSNAP was diluted to 5 μM and labeled with 10 μM benzylguanine (BG)-Alexa647 dyes (New England Biolabs) for 2 h at room temperature or overnight at 4 °C.

    Techniques: Fluorescence, Labeling, Microscopy, Binding Assay

    Fluorescent in-gel detection of SNAP-TTC labeled with different dyes. a SDS-PAGE of SNAP-TTC fusion protein labeled with SNAP-Surface® Alexa Fluor® 488 (2) or BG-647 (3), respectively. Fluorescence signals were visualized using the Maestro CRi in vivo imaging system with the appropriate filter set. b Coomassie-stained SDS gel from ( a ). The stained protein bands correspond to the measured fluorescence signals from ( a ). (1) prestained protein marker, broad range (NEB), (2) SNAP-TTC-SNAP-Surface® Alexa Fluor® 488, (3) SNAP-TTC-BG647, (4) uncoupled SNAP-TTC protein

    Journal: BMC Biotechnology

    Article Title: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen

    doi: 10.1186/s12896-016-0249-x

    Figure Lengend Snippet: Fluorescent in-gel detection of SNAP-TTC labeled with different dyes. a SDS-PAGE of SNAP-TTC fusion protein labeled with SNAP-Surface® Alexa Fluor® 488 (2) or BG-647 (3), respectively. Fluorescence signals were visualized using the Maestro CRi in vivo imaging system with the appropriate filter set. b Coomassie-stained SDS gel from ( a ). The stained protein bands correspond to the measured fluorescence signals from ( a ). (1) prestained protein marker, broad range (NEB), (2) SNAP-TTC-SNAP-Surface® Alexa Fluor® 488, (3) SNAP-TTC-BG647, (4) uncoupled SNAP-TTC protein

    Article Snippet: Coupling SNAP-TTC to the fluorescent dye Purified SNAP-TTC was conjugated to the BG-modified fluorescent dyes SNAP-surface® Alexa Fluor® 647 (New England Biolabs, Frankfurt am Main, Germany; Catalogue number: S9136S) and SNAP-surface® Alexa Fluor® 488 (New England Biolabs; Catalogue number: S9129S) as previously described [ ].

    Techniques: Labeling, SDS Page, Fluorescence, In Vivo Imaging, Staining, SDS-Gel, Marker

    Binding analysis of recombinant TTC-based proteins to TTC-reactive hybridoma cells. Equimolar amounts (100 nM) of TTC ( c ) and TTC-ETA’ ( d ) were used for binding analysis to the TTC-reactive hybridoma cell line 5E4 ( a ) compared to the control hybridoma cell line 8.18-C5 ( b ). Detection of bound proteins was carried out using an Alexa Fluor® 488-coupled anti-His5 antibody. Staining with Alexa Fluor 488-coupled anti-His5 antibody ( b ) and unstained cells ( a ) served as controls. Binding analysis of 100 nM SNAP-TTC coupled to the SNAP-Surface® 647 fluorescence dye ( b ) to 5E4 hybridoma cells ( c ) and to the control hybridoma cell line 8.18-C5 ( d ). Unstained cells served as control ( a )

    Journal: BMC Biotechnology

    Article Title: Novel fusion proteins for the antigen-specific staining and elimination of B cell receptor-positive cell populations demonstrated by a tetanus toxoid fragment C (TTC) model antigen

    doi: 10.1186/s12896-016-0249-x

    Figure Lengend Snippet: Binding analysis of recombinant TTC-based proteins to TTC-reactive hybridoma cells. Equimolar amounts (100 nM) of TTC ( c ) and TTC-ETA’ ( d ) were used for binding analysis to the TTC-reactive hybridoma cell line 5E4 ( a ) compared to the control hybridoma cell line 8.18-C5 ( b ). Detection of bound proteins was carried out using an Alexa Fluor® 488-coupled anti-His5 antibody. Staining with Alexa Fluor 488-coupled anti-His5 antibody ( b ) and unstained cells ( a ) served as controls. Binding analysis of 100 nM SNAP-TTC coupled to the SNAP-Surface® 647 fluorescence dye ( b ) to 5E4 hybridoma cells ( c ) and to the control hybridoma cell line 8.18-C5 ( d ). Unstained cells served as control ( a )

    Article Snippet: Coupling SNAP-TTC to the fluorescent dye Purified SNAP-TTC was conjugated to the BG-modified fluorescent dyes SNAP-surface® Alexa Fluor® 647 (New England Biolabs, Frankfurt am Main, Germany; Catalogue number: S9136S) and SNAP-surface® Alexa Fluor® 488 (New England Biolabs; Catalogue number: S9129S) as previously described [ ].

    Techniques: Binding Assay, Recombinant, Staining, Fluorescence