agarose  (Vector Laboratories)


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    Vector Laboratories agarose
    Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    agarose  (Vector Laboratories)


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    Vector Laboratories agarose
    Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    jacalin agarose  (Vector Laboratories)


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  • 93

    Structured Review

    Vector Laboratories jacalin agarose
    Jacalin Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin agarose/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    jacalin agarose - by Bioz Stars, 2024-05
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    jacalin lectin  (Vector Laboratories)


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    Vector Laboratories jacalin lectin
    a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to <t>jacalin</t> LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.
    Jacalin Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin lectin/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    jacalin lectin - by Bioz Stars, 2024-05
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    1) Product Images from "Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line"

    Article Title: Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line

    Journal: Nature Communications

    doi: 10.1038/s41467-022-33806-8

    a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to jacalin LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.
    Figure Legend Snippet: a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to jacalin LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.

    Techniques Used: Generated, Transduction, CRISPR, Knock-Out, Clone Assay, Purification, Labeling, Liquid Chromatography with Mass Spectroscopy

    agarose  (Vector Laboratories)


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  • 93

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    Vector Laboratories agarose
    Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 1 article reviews
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    agarose bound jacalin  (Vector Laboratories)


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    Vector Laboratories agarose bound jacalin
    Agarose Bound Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    agarose bound jacalin  (Vector Laboratories)


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    Vector Laboratories agarose bound jacalin
    Agarose Bound Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    jacalin agarose  (Vector Laboratories)


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    Vector Laboratories jacalin agarose
    Jacalin Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin agarose/product/Vector Laboratories
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    jacalin agarose  (Vector Laboratories)


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    Vector Laboratories jacalin agarose
    Jacalin Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin agarose/product/Vector Laboratories
    Average 93 stars, based on 1 article reviews
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    pna agarose  (Vector Laboratories)


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    Vector Laboratories pna agarose
    Pna Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    agarosebound jacalin  (Vector Laboratories)


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    Vector Laboratories agarosebound jacalin
    Agarosebound Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories agarose
    Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories jacalin lectin
    a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to <t>jacalin</t> LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.
    Jacalin Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin lectin/product/Vector Laboratories
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    Vector Laboratories agarose bound jacalin
    a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to <t>jacalin</t> LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.
    Agarose Bound Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories pna agarose
    a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to <t>jacalin</t> LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.
    Pna Agarose, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories agarosebound jacalin
    a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to <t>jacalin</t> LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.
    Agarosebound Jacalin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to jacalin LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.

    Journal: Nature Communications

    Article Title: Global mapping of GalNAc-T isoform-specificities and O-glycosylation site-occupancy in a tissue-forming human cell line

    doi: 10.1038/s41467-022-33806-8

    Figure Lengend Snippet: a GALNT knockouts in N/TERT-1 cell lines were generated using lentiviral transduction to deliver the CRISPR/Cas9 machinery. Total cell lysates were collected from three technical wild-type replicates and three biological replicate knockout clones, and extracted proteins were reduced, alkylated, and digested with trypsin. Purified peptides were labeled using tandem mass tags (TMT), mixed, and treated with neuraminidase prior to jacalin LWAC enrichment of glycopeptides. Finally, the enriched sample was separated into eight high pH fractions and simultaneous discovery and reporter quantification were performed using LC-MS/MS. b For the assessment of site occupancy, both total cell lysates and secreted material were obtained from three technical replicates of wild type and COSMC knockout cells. Both sample types were reduced, alkylated, and digested with trypsin followed by N -glycan removal using PNGase F. Each sample was then separated into four high pH fractions and analyzed using LC-MS/MS, where relative occupancies were calculated based on MS1 peak intensity from glycosylated peptides and their non-glycosylated equivalents.

    Article Snippet: O-glycopeptides (T and Tn) were enriched by lectin weak affinity chromatography (LWAC) using agarose-bound jacalin lectin (Vector Labs).

    Techniques: Generated, Transduction, CRISPR, Knock-Out, Clone Assay, Purification, Labeling, Liquid Chromatography with Mass Spectroscopy