Journal: Oncogene

Article Title: A causal link from ALK to hexokinase II overexpression and hyperactive glycolysis in EML4-ALK-positive lung cancer

doi: 10.1038/onc.2016.150

Figure Lengend Snippet: ( A ) The effects of ALK knockdown in H2228 and H3122 or ALK ectopic expression in A549 on levels of HIF1α mRNA were analyzed by qPCR. ( B ) H2228 and H3122 cells were treated with vehicle, ceritinib (200 nM), LY294002 (10 μM), MK2206 (4 μM), or rapamycin (20 nM) for 8 hours. HIF1α protein and mRNA levels in control and treated cells were analyzed by immunoblotting and qPCR, respectively. ( C ) AKT1/2 expression in H2228 and H3122 cells was knocked down simultaneously by siRNA targeting both isoforms. The effects on HIF1α, HK2 and other proteins as well as HIF1α mRNA were analyzed as in ( B ). In all panels, qPCR results were normalized to 18S rRNA and presented as values relative to the corresponding control cells (defined as 1 fold).

Article Snippet: AKT1/2 siRNA were purchased from Cell Signaling.

Techniques: Expressing, Western Blot