Journal: Emerging Microbes & Infections

Article Title: The feather epithelium contributes to the dissemination and ecology of clade 2.3.4.4b H5 high pathogenicity avian influenza viruses in ducks

doi: 10.1080/22221751.2023.2272644

Figure Lengend Snippet: Histological analysis of aerosols (dust) collected from H5 HPAIV-positive farms. (A) Histogel-based Coriolis Compact (Bertin Technologies) sampled dust blocks (arrows). (B–F) Dust blocks from H5 HPAIV-positive farms stained with H&E revealed the presence of frequent eosinophilic elongated structures (arrowheads), admixed with bacteria (asterisks), fungi and granular material of unknown origin. Bar, 50 µm (A) and 20 µm (B).

Article Snippet: Dust samples were collected from four H5N1 2021–2022 HPAIV-positive farms using a dry cyclonic air sampler Coriolis Compact (Bertin Technologies) for 20 min at 50 L/min.

Techniques: Staining, Bacteria

Journal: Emerging Microbes & Infections

Article Title: The feather epithelium contributes to the dissemination and ecology of clade 2.3.4.4b H5 high pathogenicity avian influenza viruses in ducks

doi: 10.1080/22221751.2023.2272644

Figure Lengend Snippet: Immunofluorescent detection of avian CBP and influenza A NP antigen in growing feather and aerosols (dust). Immunofluorescent detection of avian CBP and influenza A NP antigen in a growing feather of an H5N8/2017 experimentally infected mule duck, and in histogel-based dust block sampled by Coriolis Compact (Bertin Technologies) from four HPAIV-positive farms. 4’,6-diamino-2-phenilidole (DAPI, blue), Corneous beta-protein (CBP, green), Nucleoprotein influenza A (NP, red).

Article Snippet: Dust samples were collected from four H5N1 2021–2022 HPAIV-positive farms using a dry cyclonic air sampler Coriolis Compact (Bertin Technologies) for 20 min at 50 L/min.

Techniques: Infection, Blocking Assay