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Ht29 Lucia Ahr Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ahr signaling  (MedChemExpress)
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Activation of <t>AHR</t> alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA <t>and</t> <t>CH-223191</t> detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).
Ahr Signaling, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit anti ahr  (ABclonal Biotechnology)
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Activation of <t>AHR</t> alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA <t>and</t> <t>CH-223191</t> detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).
Rabbit Anti Ahr, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ahr  (ABclonal Biotechnology)
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Activation of <t>AHR</t> alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA <t>and</t> <t>CH-223191</t> detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).
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ahr inhibitor ch223191  (MedChemExpress)
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Activation of <t>AHR</t> alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA <t>and</t> <t>CH-223191</t> detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).
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ahr antagonist ch 223191  (MedChemExpress)
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Activation of <t>AHR</t> alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA <t>and</t> <t>CH-223191</t> detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).
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Activation of <t>AHR</t> alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA <t>and</t> <t>CH-223191</t> detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).
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hepg2-lucia ahr cells  (InvivoGen)
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Activation of <t>AHR</t> alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA <t>and</t> <t>CH-223191</t> detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).
Hepg2 Lucia Ahr Cells, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Activation of AHR alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).

Journal: Investigative Ophthalmology & Visual Science

Article Title: Gut Microbial Metabolite Indole-3-Acetic Acid Exerts Protective Effects by Regulating Orbital Fibroblast Fibrosis in Thyroid Eye Disease

doi: 10.1167/iovs.67.10.35

Figure Lengend Snippet: Activation of AHR alleviated fibrotic phenotypes by regulating THE TGF-β1/Smad2/3 signaling pathway. ( A , B ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( C ) Immunofluorescence assay measured the distribution of AHR in TED OFs ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. ( D , E ) Protein expression of fibronectin, collagen Iα1, α-SMA, AHR, and CYP1A1, and the phosphorylation of Smad2 and Smad3 in non-TED OFs treated with IAA and CH-223191 detected using a western blotting assay ( n = 3 in each group). ( F ) Immunofluorescence assay measured the distribution of AHR in non-TED OFs. ( n = 3 in each group). Blue represents DAPI, and red represents AHR. Scale bar : 20 µm. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).

Article Snippet: To evaluate the role of AHR signaling in OF activation, cells were pretreated with 10-μM AHR antagonist CH-223191 (MedChemExpress) for 2 hours before stimulation with TGF-β1 and IAA.

Techniques: Activation Assay, Expressing, Phospho-proteomics, Western Blot, Immunofluorescence

IAA attenuated cell migration by activating AHR in OFs. ( A – D ) TED OFs ( A , B ) and non-TED OFs ( C , D ) were treated with TGF-β1, IAA, and the AHR antagonist CH-223191 for 24 or 48 hours. Cell migration was examined using a scratch wound healing assay ( n = 3 in each group). Scale bar : 100 µm. * P < 0.05, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).

Journal: Investigative Ophthalmology & Visual Science

Article Title: Gut Microbial Metabolite Indole-3-Acetic Acid Exerts Protective Effects by Regulating Orbital Fibroblast Fibrosis in Thyroid Eye Disease

doi: 10.1167/iovs.67.10.35

Figure Lengend Snippet: IAA attenuated cell migration by activating AHR in OFs. ( A – D ) TED OFs ( A , B ) and non-TED OFs ( C , D ) were treated with TGF-β1, IAA, and the AHR antagonist CH-223191 for 24 or 48 hours. Cell migration was examined using a scratch wound healing assay ( n = 3 in each group). Scale bar : 100 µm. * P < 0.05, *** P < 0.001, **** P < 0.0001 compared to the TGF-β1 group (one-way ANOVA).

Article Snippet: To evaluate the role of AHR signaling in OF activation, cells were pretreated with 10-μM AHR antagonist CH-223191 (MedChemExpress) for 2 hours before stimulation with TGF-β1 and IAA.

Techniques: Migration, Wound Healing Assay