agonist forskolin  (Selleck Chemicals)


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    Selleck Chemicals agonist forskolin
    cAMP/PKA is required for the pH-dependent regulation of osteogenesis and YAP activity. BMSCs were incubated in osteogenic media for 21 days and then transferred to low pH (pH = 7.0) basal media for different times (t = 0, 5, 10, 20, or 40 min). The phosphorylation of MYPT1 and CREB was assessed by western blotting (A) . BMSCs were incubated in osteogenic media at different pHs containing a cAMP activator <t>(forskolin)</t> or a PKA inhibitor (H 89 2 HCl) for 21 days. The calcium deposits in the differentiated BMSCs were evaluated by Alizarin red S staining (B) , and the expressions of IBSP and BGLAP were examined by qRT-PCR analysis (C). * P
    Agonist Forskolin, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agonist forskolin/product/Selleck Chemicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agonist forskolin - by Bioz Stars, 2021-04
    86/100 stars

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    1) Product Images from "Decreased extracellular pH inhibits osteogenesis through proton-sensing GPR4-mediated suppression of yes-associated protein"

    Article Title: Decreased extracellular pH inhibits osteogenesis through proton-sensing GPR4-mediated suppression of yes-associated protein

    Journal: Scientific Reports

    doi: 10.1038/srep26835

    cAMP/PKA is required for the pH-dependent regulation of osteogenesis and YAP activity. BMSCs were incubated in osteogenic media for 21 days and then transferred to low pH (pH = 7.0) basal media for different times (t = 0, 5, 10, 20, or 40 min). The phosphorylation of MYPT1 and CREB was assessed by western blotting (A) . BMSCs were incubated in osteogenic media at different pHs containing a cAMP activator (forskolin) or a PKA inhibitor (H 89 2 HCl) for 21 days. The calcium deposits in the differentiated BMSCs were evaluated by Alizarin red S staining (B) , and the expressions of IBSP and BGLAP were examined by qRT-PCR analysis (C). * P
    Figure Legend Snippet: cAMP/PKA is required for the pH-dependent regulation of osteogenesis and YAP activity. BMSCs were incubated in osteogenic media for 21 days and then transferred to low pH (pH = 7.0) basal media for different times (t = 0, 5, 10, 20, or 40 min). The phosphorylation of MYPT1 and CREB was assessed by western blotting (A) . BMSCs were incubated in osteogenic media at different pHs containing a cAMP activator (forskolin) or a PKA inhibitor (H 89 2 HCl) for 21 days. The calcium deposits in the differentiated BMSCs were evaluated by Alizarin red S staining (B) , and the expressions of IBSP and BGLAP were examined by qRT-PCR analysis (C). * P

    Techniques Used: Activity Assay, Incubation, Western Blot, Staining, Quantitative RT-PCR

    Related Articles

    Fluorescence:

    Article Title: Orkambi® and amplifier co‐therapy improves function from a rare CFTR mutation in gene‐edited cells and patient tissue
    Article Snippet: Briefly, HEK‐293 cells overexpressing CFTR constructs were grown to 100% confluence in 24‐well plates (Costar) and washed with PBS, and the blue membrane potential dye (dissolved in chloride‐free buffer containing 136 mM sodium gluconate, 3 mM potassium gluconate, 10 mM glucose, 20 mM HEPES, pH 7.35, 300 mOsm, at a concentration of 0.5 mg/ml; Molecular devices), which can detect changes in transmembrane potential, was added to the cells for 1 h at 27 or 37°C as required. .. The plate was then read in a fluorescence plate reader (Molecular devices—Gemini EM) at the required temperature, and after reading the baseline fluorescence, CFTR was stimulated using the cAMP agonist forskolin (10 μM; Sigma‐Aldrich) or forskolin with VX‐770 (1 μM; Selleck Chemicals). .. CFTR‐mediated depolarization of the membrane was detected as an increase in fluorescence and repolarization or hyperpolarization as a decrease (Maitra et al, ).

    Article Title: Molecular Mechanism of Action of Trimethylangelicin Derivatives as CFTR Modulators
    Article Snippet: The plate was then read in a fluorescence plate reader (SpectraMax i3; Molecular Devices) at 37°C. .. After reading baseline fluorescence, CFTR was stimulated with the cAMP agonist forskolin (10 μM; Sigma-Aldrich) and the potentiator ivacaftor (1 μM; Selleck Chemicals). .. CFTR-mediated depolarization of membrane was detected as an increase in fluorescence, whilst repolarization or hyperpolarization was detected as a decrease ( ; ).

    Article Title: Generation of functional ciliated cholangiocytes from human pluripotent stem cells
    Article Snippet: The plate was read in a fluorescence plate reader (FLIPR® Tetra System or SpectraMax i3; Molecular Devices) at 37°C. .. After reading baseline fluorescence, CFTR was stimulated with a combination of the cAMP agonist Forskolin (10μM) and potentiators, 1μM VX770 (Selleck) or 1.5μM AP2 (X300529) (Abbvie). .. CFTR-mediated depolarization was detected as an increase in fluorescence, and repolarization was detected as a decrease with addition of 10μM CFTR-specific inhibitor CFTRInh-172 to all wells.

    Recombinant:

    Article Title: Decreased extracellular pH inhibits osteogenesis through proton-sensing GPR4-mediated suppression of yes-associated protein
    Article Snippet: The shRNA sequences used in this study were the following: shYAP#1: 5′-CTGGTCAGAGATACTTCTTAA-3′; shYAP#2: 5′-AAGCTTTGAGTTCTGACATCC-3′; shGPR4#1:5′-AAAAAAGTGCTGGCGACAGCACCTTCAACTACACCCAAGCTTCGGTGCAGCTGAAGATGCTGCCGCCAGCACGGTGTTTCGTCCTTTCCACAA-3′; shGPR4#2: Sigma SHCLND-NM_005282_. .. Inhibitor and agonist Forskolin and H 89 2HCl (H89) were obtained from Selleck (Houston, TX, USA), and recombinant human CTGF protein was purchased from Pepro Tech Inc. (Rocky Hill, NJ, USA). .. The BMSCs were cultured in osteogenic medium containing the indicated concentration of forskolin (15 μM), H 89 2HCl (20 μM) or CTGF (50 ng/mL).

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    Selleck Chemicals agonist forskolin
    cAMP/PKA is required for the pH-dependent regulation of osteogenesis and YAP activity. BMSCs were incubated in osteogenic media for 21 days and then transferred to low pH (pH = 7.0) basal media for different times (t = 0, 5, 10, 20, or 40 min). The phosphorylation of MYPT1 and CREB was assessed by western blotting (A) . BMSCs were incubated in osteogenic media at different pHs containing a cAMP activator <t>(forskolin)</t> or a PKA inhibitor (H 89 2 HCl) for 21 days. The calcium deposits in the differentiated BMSCs were evaluated by Alizarin red S staining (B) , and the expressions of IBSP and BGLAP were examined by qRT-PCR analysis (C). * P
    Agonist Forskolin, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agonist forskolin/product/Selleck Chemicals
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    agonist forskolin - by Bioz Stars, 2021-04
    86/100 stars
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    cAMP/PKA is required for the pH-dependent regulation of osteogenesis and YAP activity. BMSCs were incubated in osteogenic media for 21 days and then transferred to low pH (pH = 7.0) basal media for different times (t = 0, 5, 10, 20, or 40 min). The phosphorylation of MYPT1 and CREB was assessed by western blotting (A) . BMSCs were incubated in osteogenic media at different pHs containing a cAMP activator (forskolin) or a PKA inhibitor (H 89 2 HCl) for 21 days. The calcium deposits in the differentiated BMSCs were evaluated by Alizarin red S staining (B) , and the expressions of IBSP and BGLAP were examined by qRT-PCR analysis (C). * P

    Journal: Scientific Reports

    Article Title: Decreased extracellular pH inhibits osteogenesis through proton-sensing GPR4-mediated suppression of yes-associated protein

    doi: 10.1038/srep26835

    Figure Lengend Snippet: cAMP/PKA is required for the pH-dependent regulation of osteogenesis and YAP activity. BMSCs were incubated in osteogenic media for 21 days and then transferred to low pH (pH = 7.0) basal media for different times (t = 0, 5, 10, 20, or 40 min). The phosphorylation of MYPT1 and CREB was assessed by western blotting (A) . BMSCs were incubated in osteogenic media at different pHs containing a cAMP activator (forskolin) or a PKA inhibitor (H 89 2 HCl) for 21 days. The calcium deposits in the differentiated BMSCs were evaluated by Alizarin red S staining (B) , and the expressions of IBSP and BGLAP were examined by qRT-PCR analysis (C). * P

    Article Snippet: Inhibitor and agonist Forskolin and H 89 2HCl (H89) were obtained from Selleck (Houston, TX, USA), and recombinant human CTGF protein was purchased from Pepro Tech Inc. (Rocky Hill, NJ, USA).

    Techniques: Activity Assay, Incubation, Western Blot, Staining, Quantitative RT-PCR