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Agilent technologies agilent 2100 bioanalyser
Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an <t>Agilent</t> 2100 <t>Bioanalyser.</t> Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.
Agilent 2100 Bioanalyser, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/agilent 2100 bioanalyser/product/Agilent technologies
Average 92 stars, based on 7 article reviews
Price from $9.99 to $1999.99
agilent 2100 bioanalyser - by Bioz Stars, 2020-05
92/100 stars

Images

1) Product Images from "Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae"

Article Title: Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae

Journal: BMC Genomics

doi: 10.1186/1471-2164-15-854

Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an Agilent 2100 Bioanalyser. Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.
Figure Legend Snippet: Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Isolation of cotton tissues from paraffin-embedded sections by laser microdissection (LMD). Sections before LMD (a, c, e) , and sections after LM (b, d, f) . The area selected for laser microdissection is outlined in green (a region near the damage caused by larvae feeding, which comprised the stamen tissue, viewed at a , c and d ) or blue (a region farther from the injured area, which comprised the carpel tissue, viewed at a , e , and f ). The assessment of extracted RNA integrity from the stamen and carpel are shown in g and h , respectively. Electropherograms were obtained with an Agilent 2100 Bioanalyser. Open and closed arrowheads indicate the 18S and 28S ribosomal RNA peaks, respectively. RNA quality is expressed as the RNA integrity number (RIN). Scale bars = 100 μm.

Techniques Used: Isolation, Laser Capture Microdissection

2) Product Images from "Regulatory T cell-derived extracellular vesicles modify dendritic cell function"

Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function

Journal: Scientific Reports

doi: 10.1038/s41598-018-24531-8

Murine CD4 + CD25 + Treg EVs contain different miRNA compared to control T cell EVs. ( A) dTreg and control FoxP3 - T cell EVs (n = 3 per group) isolated by ExoQuick-TC were lysed and total RNA was purified and assessed using NanoDrop™ spectrophotometer and Agilent 2100 Bioanalyser. EVs miRNA was reverse transcribed into cDNA and pre-amplified before miRNA detection using QuantStudio™ 12 K Flex Real-Time PCR System with the OpenArray® Platform. The volcano plot shows the relation between the p-value and the log fold change between FoxP3 − T cell and Treg EV miRNA expression levels. The blue line indicates the inverse log 10 of the p-value = 0.05. ( B ) Bar graphs (mean + SEM) showing the relative quantity of miR-142-3p, miR-150-5p, and miR-384-5p by control T cell EVs (Control EVs) and Treg EVs (Treg EVs) as measured by qPCR and normalised relative to RNU6-2. Data pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using a two-tailed Student’s t-test where *p
Figure Legend Snippet: Murine CD4 + CD25 + Treg EVs contain different miRNA compared to control T cell EVs. ( A) dTreg and control FoxP3 - T cell EVs (n = 3 per group) isolated by ExoQuick-TC were lysed and total RNA was purified and assessed using NanoDrop™ spectrophotometer and Agilent 2100 Bioanalyser. EVs miRNA was reverse transcribed into cDNA and pre-amplified before miRNA detection using QuantStudio™ 12 K Flex Real-Time PCR System with the OpenArray® Platform. The volcano plot shows the relation between the p-value and the log fold change between FoxP3 − T cell and Treg EV miRNA expression levels. The blue line indicates the inverse log 10 of the p-value = 0.05. ( B ) Bar graphs (mean + SEM) showing the relative quantity of miR-142-3p, miR-150-5p, and miR-384-5p by control T cell EVs (Control EVs) and Treg EVs (Treg EVs) as measured by qPCR and normalised relative to RNU6-2. Data pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using a two-tailed Student’s t-test where *p

Techniques Used: Isolation, Purification, Spectrophotometry, Amplification, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test

3) Product Images from "Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids"

Article Title: Retrieval of entire genes from environmental DNA by inverse PCR with pre-amplification of target genes using primers containing locked nucleic acids

Journal: Environmental Microbiology

doi: 10.1111/j.1462-2920.2007.01518.x

Capillary electrophoreses of IPCR (odd-numbered lanes) and PAI-PCR (even-numbered lanes) products amplified from DNA extracted from horse vermiform appendixes. The electrophoreses followed by the analysis of the electrophoregrams were automatically performed by using an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio). The results are shown as a gel-like image. L is the ladder obtained with DNA fragment size markers (bp). The green and pink peaks are the lowest 50 bp and highest 10 380 bp markers respectively. The substrates used for IPCR or PAI-PCR were BamHI-digested DNA (lanes 1 and 2), EcoRI-digested DNA (lanes 3 and 4), HindIII-digested DNA (lanes 5 and 6), PstI-digested DNA (lanes 7 and 8) and XbaI-digested DNA (lanes 9 and 10).
Figure Legend Snippet: Capillary electrophoreses of IPCR (odd-numbered lanes) and PAI-PCR (even-numbered lanes) products amplified from DNA extracted from horse vermiform appendixes. The electrophoreses followed by the analysis of the electrophoregrams were automatically performed by using an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio). The results are shown as a gel-like image. L is the ladder obtained with DNA fragment size markers (bp). The green and pink peaks are the lowest 50 bp and highest 10 380 bp markers respectively. The substrates used for IPCR or PAI-PCR were BamHI-digested DNA (lanes 1 and 2), EcoRI-digested DNA (lanes 3 and 4), HindIII-digested DNA (lanes 5 and 6), PstI-digested DNA (lanes 7 and 8) and XbaI-digested DNA (lanes 9 and 10).

Techniques Used: Polymerase Chain Reaction, Amplification

Capillary electrophoreses of PAI-PCR products amplified from DNA extracted from 12 different termite gut samples. All DNA samples were digested with EcoRI, and an RCA primer [(A) Xyn-RC(LNA-Even); or (B) Xyn-RC(LNA-5′)] was used in the RCA reaction. Products were analysed with an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio).
Figure Legend Snippet: Capillary electrophoreses of PAI-PCR products amplified from DNA extracted from 12 different termite gut samples. All DNA samples were digested with EcoRI, and an RCA primer [(A) Xyn-RC(LNA-Even); or (B) Xyn-RC(LNA-5′)] was used in the RCA reaction. Products were analysed with an Agilent 2100 Bioanalyser with a DNA 7500 LabChip® kit (TaKaRa-Bio).

Techniques Used: Polymerase Chain Reaction, Amplification

4) Product Images from "Novel zinc-based fixative for high quality DNA, RNA and protein analysis"

Article Title: Novel zinc-based fixative for high quality DNA, RNA and protein analysis

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkm433

Assessment of mouse liver RNA quality using the Agilent 2100 Bioanalyser. ( a ) Typical graphs showing severely degraded, partially degraded and intact RNA. ( b ) Graphs showing RNA quality from tissues fixed in: NBF, Z2, Z7 and also fresh-frozen mouse liver.
Figure Legend Snippet: Assessment of mouse liver RNA quality using the Agilent 2100 Bioanalyser. ( a ) Typical graphs showing severely degraded, partially degraded and intact RNA. ( b ) Graphs showing RNA quality from tissues fixed in: NBF, Z2, Z7 and also fresh-frozen mouse liver.

Techniques Used:

5) Product Images from "‘Degraded’ RNA profiles in Arthropoda and beyond"

Article Title: ‘Degraded’ RNA profiles in Arthropoda and beyond

Journal: PeerJ

doi: 10.7717/peerj.1436

Electropherogram traces for 100–200 ng of total RNA applied to an RNA Nano Chip were generated on the Agilent 2100 Bioanalyser. RNA that appears ‘degraded’ after heat-denaturation and fails to provide a RNA Integrity Number (RIN) can generate high RINs in non-heat-denatured aliquots from the same RNA stock. RIN numbers are shown in each case for (A) Spider Grammostola porteri ; (B) Centipede Scolopendra subspinipes ; (C) Stalked barnacle, Order Lepadiformes, Lepas anatifera ; D. Stalked barnacle, Order Lepadiformes, Dosima fascicularis E. Stalked barnacle, Order Scalpelliformes, Pollicipes pollicipes .
Figure Legend Snippet: Electropherogram traces for 100–200 ng of total RNA applied to an RNA Nano Chip were generated on the Agilent 2100 Bioanalyser. RNA that appears ‘degraded’ after heat-denaturation and fails to provide a RNA Integrity Number (RIN) can generate high RINs in non-heat-denatured aliquots from the same RNA stock. RIN numbers are shown in each case for (A) Spider Grammostola porteri ; (B) Centipede Scolopendra subspinipes ; (C) Stalked barnacle, Order Lepadiformes, Lepas anatifera ; D. Stalked barnacle, Order Lepadiformes, Dosima fascicularis E. Stalked barnacle, Order Scalpelliformes, Pollicipes pollicipes .

Techniques Used: Chromatin Immunoprecipitation, Generated

6) Product Images from "Novel zinc-based fixative for high quality DNA, RNA and protein analysis"

Article Title: Novel zinc-based fixative for high quality DNA, RNA and protein analysis

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkm433

Assessment of mouse liver RNA quality using the Agilent 2100 Bioanalyser. ( a ) Typical graphs showing severely degraded, partially degraded and intact RNA. ( b ) Graphs showing RNA quality from tissues fixed in: NBF, Z2, Z7 and also fresh-frozen mouse liver.
Figure Legend Snippet: Assessment of mouse liver RNA quality using the Agilent 2100 Bioanalyser. ( a ) Typical graphs showing severely degraded, partially degraded and intact RNA. ( b ) Graphs showing RNA quality from tissues fixed in: NBF, Z2, Z7 and also fresh-frozen mouse liver.

Techniques Used:

7) Product Images from "Novel zinc-based fixative for high quality DNA, RNA and protein analysis"

Article Title: Novel zinc-based fixative for high quality DNA, RNA and protein analysis

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkm433

Assessment of mouse liver RNA quality using the Agilent 2100 Bioanalyser. ( a ) Typical graphs showing severely degraded, partially degraded and intact RNA. ( b ) Graphs showing RNA quality from tissues fixed in: NBF, Z2, Z7 and also fresh-frozen mouse liver.
Figure Legend Snippet: Assessment of mouse liver RNA quality using the Agilent 2100 Bioanalyser. ( a ) Typical graphs showing severely degraded, partially degraded and intact RNA. ( b ) Graphs showing RNA quality from tissues fixed in: NBF, Z2, Z7 and also fresh-frozen mouse liver.

Techniques Used:

8) Product Images from "‘Degraded’ RNA profiles in Arthropoda and beyond"

Article Title: ‘Degraded’ RNA profiles in Arthropoda and beyond

Journal: PeerJ

doi: 10.7717/peerj.1436

Electropherogram traces for 100–200 ng of total RNA applied to an RNA Nano Chip were generated on the Agilent 2100 Bioanalyser. RNA that appears ‘degraded’ after heat-denaturation and fails to provide a RNA Integrity Number (RIN) can generate high RINs in non-heat-denatured aliquots from the same RNA stock. RIN numbers are shown in each case for (A) Spider Grammostola porteri ; (B) Centipede Scolopendra subspinipes ; (C) Stalked barnacle, Order Lepadiformes, Lepas anatifera ; D. Stalked barnacle, Order Lepadiformes, Dosima fascicularis E. Stalked barnacle, Order Scalpelliformes, Pollicipes pollicipes .
Figure Legend Snippet: Electropherogram traces for 100–200 ng of total RNA applied to an RNA Nano Chip were generated on the Agilent 2100 Bioanalyser. RNA that appears ‘degraded’ after heat-denaturation and fails to provide a RNA Integrity Number (RIN) can generate high RINs in non-heat-denatured aliquots from the same RNA stock. RIN numbers are shown in each case for (A) Spider Grammostola porteri ; (B) Centipede Scolopendra subspinipes ; (C) Stalked barnacle, Order Lepadiformes, Lepas anatifera ; D. Stalked barnacle, Order Lepadiformes, Dosima fascicularis E. Stalked barnacle, Order Scalpelliformes, Pollicipes pollicipes .

Techniques Used: Chromatin Immunoprecipitation, Generated

9) Product Images from "Novel zinc-based fixative for high quality DNA, RNA and protein analysis"

Article Title: Novel zinc-based fixative for high quality DNA, RNA and protein analysis

Journal: Nucleic Acids Research

doi: 10.1093/nar/gkm433

Assessment of mouse liver RNA quality using the Agilent 2100 Bioanalyser. ( a ) Typical graphs showing severely degraded, partially degraded and intact RNA. ( b ) Graphs showing RNA quality from tissues fixed in: NBF, Z2, Z7 and also fresh-frozen mouse liver.
Figure Legend Snippet: Assessment of mouse liver RNA quality using the Agilent 2100 Bioanalyser. ( a ) Typical graphs showing severely degraded, partially degraded and intact RNA. ( b ) Graphs showing RNA quality from tissues fixed in: NBF, Z2, Z7 and also fresh-frozen mouse liver.

Techniques Used:

10) Product Images from "Comparison of RNA Isolation Methods From Insect Larvae"

Article Title: Comparison of RNA Isolation Methods From Insect Larvae

Journal: Journal of Insect Science

doi: 10.1093/jisesa/ieu130

Agilent 2100 Bioanalyser electropherogram graphs showing RNA extracted using the RNeasy Mini Kit, a CTAB-based protocol, TRIzol reagent and the SV Total RNA kit Markers can be seen at 25 nt, 18S RNA subunit at 2,000 nt, the 28S large subunit at 3,800 nt, and a combination of 5S, 5.8S, and tRNAs at ± 180 nt.
Figure Legend Snippet: Agilent 2100 Bioanalyser electropherogram graphs showing RNA extracted using the RNeasy Mini Kit, a CTAB-based protocol, TRIzol reagent and the SV Total RNA kit Markers can be seen at 25 nt, 18S RNA subunit at 2,000 nt, the 28S large subunit at 3,800 nt, and a combination of 5S, 5.8S, and tRNAs at ± 180 nt.

Techniques Used:

Pseudo-gel image produced using an Agilent 2100 Bioanalyser, showing the results of RNA extracted from T. leucotreta using the RNeasy Mini Kit, a CTAB-based protocol, TRIzol reagent, and the SV Total RNA kit. The 18S RNA subunit is visible at 2,000 nt, the 28S large subunit at 3,800 nt, and a combination of 5S, 5.8S, and tRNAs at ± 180 nt.
Figure Legend Snippet: Pseudo-gel image produced using an Agilent 2100 Bioanalyser, showing the results of RNA extracted from T. leucotreta using the RNeasy Mini Kit, a CTAB-based protocol, TRIzol reagent, and the SV Total RNA kit. The 18S RNA subunit is visible at 2,000 nt, the 28S large subunit at 3,800 nt, and a combination of 5S, 5.8S, and tRNAs at ± 180 nt.

Techniques Used: Produced

11) Product Images from "Regulatory T cell-derived extracellular vesicles modify dendritic cell function"

Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function

Journal: Scientific Reports

doi: 10.1038/s41598-018-24531-8

Murine CD4 + CD25 + Treg EVs contain different miRNA compared to control T cell EVs. ( A) dTreg and control FoxP3 - T cell EVs (n = 3 per group) isolated by ExoQuick-TC were lysed and total RNA was purified and assessed using NanoDrop™ spectrophotometer and Agilent 2100 Bioanalyser. EVs miRNA was reverse transcribed into cDNA and pre-amplified before miRNA detection using QuantStudio™ 12 K Flex Real-Time PCR System with the OpenArray® Platform. The volcano plot shows the relation between the p-value and the log fold change between FoxP3 − T cell and Treg EV miRNA expression levels. The blue line indicates the inverse log 10 of the p-value = 0.05. ( B ) Bar graphs (mean + SEM) showing the relative quantity of miR-142-3p, miR-150-5p, and miR-384-5p by control T cell EVs (Control EVs) and Treg EVs (Treg EVs) as measured by qPCR and normalised relative to RNU6-2. Data pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using a two-tailed Student’s t-test where *p
Figure Legend Snippet: Murine CD4 + CD25 + Treg EVs contain different miRNA compared to control T cell EVs. ( A) dTreg and control FoxP3 - T cell EVs (n = 3 per group) isolated by ExoQuick-TC were lysed and total RNA was purified and assessed using NanoDrop™ spectrophotometer and Agilent 2100 Bioanalyser. EVs miRNA was reverse transcribed into cDNA and pre-amplified before miRNA detection using QuantStudio™ 12 K Flex Real-Time PCR System with the OpenArray® Platform. The volcano plot shows the relation between the p-value and the log fold change between FoxP3 − T cell and Treg EV miRNA expression levels. The blue line indicates the inverse log 10 of the p-value = 0.05. ( B ) Bar graphs (mean + SEM) showing the relative quantity of miR-142-3p, miR-150-5p, and miR-384-5p by control T cell EVs (Control EVs) and Treg EVs (Treg EVs) as measured by qPCR and normalised relative to RNU6-2. Data pooled from 3 individual experiments that were performed in triplicates. Statistical significance was determined using a two-tailed Student’s t-test where *p

Techniques Used: Isolation, Purification, Spectrophotometry, Amplification, Real-time Polymerase Chain Reaction, Expressing, Two Tailed Test

12) Product Images from "Comparison of RNA Isolation Methods From Insect Larvae"

Article Title: Comparison of RNA Isolation Methods From Insect Larvae

Journal: Journal of Insect Science

doi: 10.1093/jisesa/ieu130

Agilent 2100 Bioanalyser electropherogram graphs showing RNA extracted using the RNeasy Mini Kit, a CTAB-based protocol, TRIzol reagent and the SV Total RNA kit Markers can be seen at 25 nt, 18S RNA subunit at 2,000 nt, the 28S large subunit at 3,800 nt, and a combination of 5S, 5.8S, and tRNAs at ± 180 nt.
Figure Legend Snippet: Agilent 2100 Bioanalyser electropherogram graphs showing RNA extracted using the RNeasy Mini Kit, a CTAB-based protocol, TRIzol reagent and the SV Total RNA kit Markers can be seen at 25 nt, 18S RNA subunit at 2,000 nt, the 28S large subunit at 3,800 nt, and a combination of 5S, 5.8S, and tRNAs at ± 180 nt.

Techniques Used:

Pseudo-gel image produced using an Agilent 2100 Bioanalyser, showing the results of RNA extracted from T. leucotreta using the RNeasy Mini Kit, a CTAB-based protocol, TRIzol reagent, and the SV Total RNA kit. The 18S RNA subunit is visible at 2,000 nt, the 28S large subunit at 3,800 nt, and a combination of 5S, 5.8S, and tRNAs at ± 180 nt.
Figure Legend Snippet: Pseudo-gel image produced using an Agilent 2100 Bioanalyser, showing the results of RNA extracted from T. leucotreta using the RNeasy Mini Kit, a CTAB-based protocol, TRIzol reagent, and the SV Total RNA kit. The 18S RNA subunit is visible at 2,000 nt, the 28S large subunit at 3,800 nt, and a combination of 5S, 5.8S, and tRNAs at ± 180 nt.

Techniques Used: Produced

Related Articles

Real-time Polymerase Chain Reaction:

Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
Article Snippet: .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system. ..

Agarose Gel Electrophoresis:

Article Title: Novel zinc-based fixative for high quality DNA, RNA and protein analysis
Article Snippet: .. In the current study, the quality of RNA was assessed using the Agilent 2100 Bioanalyser and agarose gel electrophoresis. .. Our initial RT–PCR and Real-Time RT–PCR analyses demonstrated that amplicons up to 361 bp in size could be consistently obtained using our methodology, in direct contrast to the results obtained using NBF-fixed tissue, and comparable to fresh-frozen material.

Laser Capture Microdissection:

Article Title: Transcriptome analysis of Gossypium hirsutum flower buds infested by cotton boll weevil (Anthonomus grandis) larvae
Article Snippet: .. The quality of total RNA extracted from LMD-collected tissues was assessed using an RNA 6000 Pico kit on the Agilent 2100 Bioanalyser (Agilent Technologies). ..

Spectrophotometry:

Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
Article Snippet: .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system. ..

Article Title: Novel zinc-based fixative for high quality DNA, RNA and protein analysis
Article Snippet: .. RNA concentrations from each fixed tissue was measured using a Nanodrop 1000 spectrophotometer and these together with the results from the Agilent 2100 Bioanalyser showed that the quality of extracted RNA was inferior to that extracted from the zinc-based fixative Z7 (data not shown). ..

SYBR Green Assay:

Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
Article Snippet: .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system. ..

Polymerase Chain Reaction:

Article Title: Regulatory T cell-derived extracellular vesicles modify dendritic cell function
Article Snippet: .. Total RNA profile was assessed using an Agilent 2100 Bioanalyser and total RNA nano chips and quantified by NanoDrop™ spectrophotometer (Thermo Fisher Scientific). cDNA fragments were synthesised using the ‘miScript RT Kit’ (Qiagen, Hilden, Germany), according to manufacturer’s protocols. qPCR reactions were set up with the miScript SYBR Green PCR Kit and primers specific for miR-125b-5p, miR-29a-3p, miR-182-5p, miR-142-3p, miR-150-5p, miR-384-5p, SCARNA17 and RNU6-2, as per manufacturer’s protocols and run on the Applied Biosystems™ ViiA™ 7 Real time PCR system. ..

Chromatin Immunoprecipitation:

Article Title: ‘Degraded’ RNA profiles in Arthropoda and beyond
Article Snippet: .. For the analyses of quality and integrity, RNA (100–200 ng) was electrophoretically separated with an Agilent 2100 Bioanalyser using an RNA 6000 Nano Chip Kit according to manufacturer’s instructions. ..

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    Agilent technologies agilent 2100 bioanalyzer
    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using <t>Agilent</t> 2100 <t>Bioanalyzer</t> with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.
    Agilent 2100 Bioanalyzer, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 99/100, based on 2142 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agilent 2100 bioanalyzer/product/Agilent technologies
    Average 99 stars, based on 2142 article reviews
    Price from $9.99 to $1999.99
    agilent 2100 bioanalyzer - by Bioz Stars, 2020-05
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    Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.

    Journal: BMC Research Notes

    Article Title: Ensuring good quality rna for quantitative real-time pcr isolated from renal proximal tubular cells using laser capture microdissection

    doi: 10.1186/1756-0500-7-62

    Figure Lengend Snippet: Total RNA concentrations from different number of renal cells. Total RNA concentrations were measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit. It was observed that 3000 renal cells yielded a total RNA concentration too low to be detected while 8000 and 18000 renal cells produced similar total RNA yields.

    Article Snippet: RNA integrity number (RIN) RIN was measured using Agilent 2100 Bioanalyzer with Agilent RNA 6000 Pico Kit.

    Techniques: Concentration Assay, Produced

    Analyses of the various antibody formats ( A ) To assess the purity and confirm the size of each reagent, the various antibody formats were analyzed by Agilent 2100 bioanalyzer. Proteins were assessed under non-reduced (NR) and reduced conditions (R). 1, IgG; 2, heavy chain; 3, light chain; 4, F(ab)’ 2 ; 5, VH-CH1-hinge; 6, monovalent antibody; 7, hinge-Fc; 8, Fab; 9, light chain and VH-CH1. ( B ) The binding of Hu 15C1 (black circles), monovalent Hu 15C1 (gray triangles), F(ab)’ 2 Hu 15C1 (open circles) and Fab Hu 15C1 (open diamonds) to TLR4 was analyzed by competitive ELISA. To compare the different antibody formats, the same number of binding site was used, i.e., the molar concentration of monovalent and Fab is twice the molar concentration of IgG and F(ab)’ 2 . Results are normalized and expressed as mean ± SD of duplicates. An F test was used to compare the fitted curves of different groups. ns: not significant.

    Journal: mAbs

    Article Title: Maximizing the potency of an anti-TLR4 monoclonal antibody by exploiting proximity to Fcγ receptors

    doi: 10.4161/19420862.2014.975098

    Figure Lengend Snippet: Analyses of the various antibody formats ( A ) To assess the purity and confirm the size of each reagent, the various antibody formats were analyzed by Agilent 2100 bioanalyzer. Proteins were assessed under non-reduced (NR) and reduced conditions (R). 1, IgG; 2, heavy chain; 3, light chain; 4, F(ab)’ 2 ; 5, VH-CH1-hinge; 6, monovalent antibody; 7, hinge-Fc; 8, Fab; 9, light chain and VH-CH1. ( B ) The binding of Hu 15C1 (black circles), monovalent Hu 15C1 (gray triangles), F(ab)’ 2 Hu 15C1 (open circles) and Fab Hu 15C1 (open diamonds) to TLR4 was analyzed by competitive ELISA. To compare the different antibody formats, the same number of binding site was used, i.e., the molar concentration of monovalent and Fab is twice the molar concentration of IgG and F(ab)’ 2 . Results are normalized and expressed as mean ± SD of duplicates. An F test was used to compare the fitted curves of different groups. ns: not significant.

    Article Snippet: The purity and chain composition of each reagent was assessed using an Agilent 2100 bioanalyzer ( ).

    Techniques: Binding Assay, Competitive ELISA, Concentration Assay

    Long-term storage effects on RNA integrity. RIN values for RNA samples isolated from Tempus tubes stored at –80°C until analyzed by Agilent 2100 Bioanalyzer. RIN values for adult blood samples (n = 15 Tempus tubes/year) and RIN values for cord blood samples (n = 6 Tempus tubes/year). Bars represent means ± SE. The average RIN values for adult and cord blood samples were 7.6 ± 0.5 and 7.7 ± 0.7, respectively, and no significant long-term storage related effects on RNA integrity were observed.

    Journal: BMC Research Notes

    Article Title: Long-term storage of blood RNA collected in RNA stabilizing Tempus tubes in a large biobank – evaluation of RNA quality and stability

    doi: 10.1186/1756-0500-7-633

    Figure Lengend Snippet: Long-term storage effects on RNA integrity. RIN values for RNA samples isolated from Tempus tubes stored at –80°C until analyzed by Agilent 2100 Bioanalyzer. RIN values for adult blood samples (n = 15 Tempus tubes/year) and RIN values for cord blood samples (n = 6 Tempus tubes/year). Bars represent means ± SE. The average RIN values for adult and cord blood samples were 7.6 ± 0.5 and 7.7 ± 0.7, respectively, and no significant long-term storage related effects on RNA integrity were observed.

    Article Snippet: The RNA integrity was assessed by an Agilent 2100 Bioanalyzer using the Eukaryote total RNA 6000 Nano LabChip kit and Eukaryote total RNA Nano assay according to the manufacturer’s instructions (Agilent Technologies, Palo Alto, CA).

    Techniques: Isolation