Journal: Molecular Cell

Article Title: Genomic RNA Elements Drive Phase Separation of the SARS-CoV-2 Nucleocapsid

doi: 10.1016/j.molcel.2020.11.041

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Article Snippet: Plasmids and sequences used: To create pET30b-6xHis-TEV-Nucleocapsid, the N-protein coding sequence (28273-29533 nt) preceded by the ORFN subgenomic 5′-UTR (1-75 nt) (Plasmid pUC57-2019-ncov, kind gift from Tim Sheahan and Ralph Baric) was cloned into AGB1329 (pET30b-6xHis-TEV) using SALI (NEB R3138S) and NOTI (NEB R3189S) restriction cloning with addition of the restriction sites to pUC57-2019-ncov by PCR using Sal-N-protein-fw (acgcatcgtcgacATGTCTGATAATGGACC-CCAAAATCAG) and Not1-N-protein-rev (tatctatgcggccgcTTAGGCCTGAGTTGAGTCAGC).

Techniques: Recombinant, Protease Inhibitor, Plasmid Preparation, Software, Sequencing