agarose  (TaKaRa)

 
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    Name:
    Agarose L03
    Description:
    Agarose a polysaccharide obtained from agar is used for a variety of molecular biology applications Agarose L03 has been purified to remove contaminants and is intended for use during gel electrophoresis to separate DNA fragments that are greater than or equal to 1 000 bp
    Catalog Number:
    5003
    Price:
    None
    Size:
    100 g
    Category:
    Agarose L03 Agarose gel powders Agarose gel electrophoresis Cloning
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    Structured Review

    TaKaRa agarose
    Agarose a polysaccharide obtained from agar is used for a variety of molecular biology applications Agarose L03 has been purified to remove contaminants and is intended for use during gel electrophoresis to separate DNA fragments that are greater than or equal to 1 000 bp
    https://www.bioz.com/result/agarose/product/TaKaRa
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    agarose - by Bioz Stars, 2020-11
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Occurrence of mitochondrial CO1 pseudogenes in Neocalanus plumchrus (Crustacea: Copepoda): Hybridization indicated by recombined nuclear mitochondrial pseudogenes
    Article Snippet: .. Cloning Prior to cloning, PCR amplicons from gDNA and cDNA were gel-purified on a 1.0% L 03 agarose gel (Takara Bio Inc.). .. The bands were excised and treated with the Wizard SV Gel and PCR Clean-Up System (Promega Inc.).

    Agarose Gel Electrophoresis:

    Article Title: Inter-Individual Differences in the Oral Bacteriome Are Greater than Intra-Day Fluctuations in Individuals
    Article Snippet: .. Samples were then pooled in equal amounts, purified by 2% L03 agarose gel (TaKaRa) electrophoresis followed by extraction using a MinElute Gel Extraction Kit (Qiagen, Hilden, Germany), and then re-quantified using the Qubit fluorometer. .. Finally, 10 pM of the pooled sample was sequenced by 250 bp paired-end sequencing protocol using the MiSeq sequencing reagent kit v2 (Illumina) according to the manufacturer's instructions.

    Article Title: Occurrence of mitochondrial CO1 pseudogenes in Neocalanus plumchrus (Crustacea: Copepoda): Hybridization indicated by recombined nuclear mitochondrial pseudogenes
    Article Snippet: .. Cloning Prior to cloning, PCR amplicons from gDNA and cDNA were gel-purified on a 1.0% L 03 agarose gel (Takara Bio Inc.). .. The bands were excised and treated with the Wizard SV Gel and PCR Clean-Up System (Promega Inc.).

    Purification:

    Article Title: Inter-Individual Differences in the Oral Bacteriome Are Greater than Intra-Day Fluctuations in Individuals
    Article Snippet: .. Samples were then pooled in equal amounts, purified by 2% L03 agarose gel (TaKaRa) electrophoresis followed by extraction using a MinElute Gel Extraction Kit (Qiagen, Hilden, Germany), and then re-quantified using the Qubit fluorometer. .. Finally, 10 pM of the pooled sample was sequenced by 250 bp paired-end sequencing protocol using the MiSeq sequencing reagent kit v2 (Illumina) according to the manufacturer's instructions.

    Electrophoresis:

    Article Title: Inter-Individual Differences in the Oral Bacteriome Are Greater than Intra-Day Fluctuations in Individuals
    Article Snippet: .. Samples were then pooled in equal amounts, purified by 2% L03 agarose gel (TaKaRa) electrophoresis followed by extraction using a MinElute Gel Extraction Kit (Qiagen, Hilden, Germany), and then re-quantified using the Qubit fluorometer. .. Finally, 10 pM of the pooled sample was sequenced by 250 bp paired-end sequencing protocol using the MiSeq sequencing reagent kit v2 (Illumina) according to the manufacturer's instructions.

    other:

    Article Title: Rapid detection of Salmonella enterica serovar Typhimurium DT104 strains by the polymerase chain reaction
    Article Snippet: The reaction mixtures were separated by 2 % agarose gel electrophoresis (Agarose L03; Takara Bio).

    Article Title: New PCR primers for metabarcoding environmental DNA from freshwater eels, genus Anguilla
    Article Snippet: The PCR products were electrophoresed on 2% agarose gel (L03; Takara, Otsu, Japan) to check the amplifications.

    Polymerase Chain Reaction:

    Article Title: Occurrence of mitochondrial CO1 pseudogenes in Neocalanus plumchrus (Crustacea: Copepoda): Hybridization indicated by recombined nuclear mitochondrial pseudogenes
    Article Snippet: .. Cloning Prior to cloning, PCR amplicons from gDNA and cDNA were gel-purified on a 1.0% L 03 agarose gel (Takara Bio Inc.). .. The bands were excised and treated with the Wizard SV Gel and PCR Clean-Up System (Promega Inc.).

    Gel Extraction:

    Article Title: Inter-Individual Differences in the Oral Bacteriome Are Greater than Intra-Day Fluctuations in Individuals
    Article Snippet: .. Samples were then pooled in equal amounts, purified by 2% L03 agarose gel (TaKaRa) electrophoresis followed by extraction using a MinElute Gel Extraction Kit (Qiagen, Hilden, Germany), and then re-quantified using the Qubit fluorometer. .. Finally, 10 pM of the pooled sample was sequenced by 250 bp paired-end sequencing protocol using the MiSeq sequencing reagent kit v2 (Illumina) according to the manufacturer's instructions.

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  • 94
    TaKaRa agarose gel dna purification kit
    Co-culture of XENO-1 islets and HEK293 cells. Examination of PERV infectivity in Co-culture is demonstrated here where detection of PERV-pol mRNA in HEK-293 co-cultured with islets from xeno-1 animals by <t>RT-PCR.</t> M, 50 <t>bp-DNA</t> ladder; +, positive control, using DNA from HEK293 co-cultured with PK15; −, negative control, HEK293 cell cultured alone; co indicates HEK-293 cells co-cultured with XENO-1 islets.
    Agarose Gel Dna Purification Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 248 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agarose gel dna purification kit/product/TaKaRa
    Average 94 stars, based on 248 article reviews
    Price from $9.99 to $1999.99
    agarose gel dna purification kit - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

    92
    TaKaRa gfp trap agarose beads
    MoVps35 interacts with MoAtg8 and is involved in the retrieval of MoAtg8. (A) <t>GFP-trap</t> based pull down experiment indicating the interaction between MoVps35-GFP and RFP-MoAtg8 in the transformant. Total proteins isolated from the wild-type strain (WT) are included as a negative control. MoVps35-GFP shows specific interaction with the cleaved and lipidated MoAtg8 during MM-N induced starvation. Top, middle, and bottom images represent <t>immunoblot</t> detection with anti-RFP, anti-GFP, and anti-actin antibodies, respectively, as indicated. (B) Delayed post-translational processing of MoAtg8 in ΔMovps35 mutant. Immunoblot analysis of total lysates from CM and MM-N cultured (3 h to 9 h in starvation environment) ΔMoatg8 RFP-MoATG8 and ΔMovps35 RFP-MoATG8 transformants, with anti-RFP antibody. Coomassie blue staining of total lysates serves as a loading control.
    Gfp Trap Agarose Beads, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gfp trap agarose beads/product/TaKaRa
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    gfp trap agarose beads - by Bioz Stars, 2020-11
    92/100 stars
      Buy from Supplier

    90
    TaKaRa anti flag antibody conjugated agarose beads
    Workflow of the SMV-based gene delivery for identification of cellular interacting protein partners. Schematic representation of SMV recombinant constructs shows in-frame insertion of the <t>Flag-tagged</t> GFP and Flag-tagged HC-Pro into the P1/HC-Pro gene insertion cassette of pSMV-Dual vectors. Each SMV recombinant construct was rub-inoculated on the leaves of soybean seedlings. At 15 dpi, crude plant extracts prepared by homogenizing the upper symptomatic leaves were subjected to immunoprecipitation using <t>anti-Flag</t> <t>antibody-conjugated</t> <t>agarose</t> <t>beads.</t> The resulting co-immunoprecipitated products were analyzed by SDS-PAGE and the bands of interest (indicated by asterisks) were excised from the gel and subjected to LC-MS/MS analysis. The identified proteins and MS/MS spectral information are shown.
    Anti Flag Antibody Conjugated Agarose Beads, supplied by TaKaRa, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti flag antibody conjugated agarose beads/product/TaKaRa
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti flag antibody conjugated agarose beads - by Bioz Stars, 2020-11
    90/100 stars
      Buy from Supplier

    Image Search Results


    Co-culture of XENO-1 islets and HEK293 cells. Examination of PERV infectivity in Co-culture is demonstrated here where detection of PERV-pol mRNA in HEK-293 co-cultured with islets from xeno-1 animals by RT-PCR. M, 50 bp-DNA ladder; +, positive control, using DNA from HEK293 co-cultured with PK15; −, negative control, HEK293 cell cultured alone; co indicates HEK-293 cells co-cultured with XENO-1 islets.

    Journal: Virology Journal

    Article Title: Characterization of PERV in a new conserved pig herd as potential donor animals for xenotransplantation in China

    doi: 10.1186/s12985-014-0212-1

    Figure Lengend Snippet: Co-culture of XENO-1 islets and HEK293 cells. Examination of PERV infectivity in Co-culture is demonstrated here where detection of PERV-pol mRNA in HEK-293 co-cultured with islets from xeno-1 animals by RT-PCR. M, 50 bp-DNA ladder; +, positive control, using DNA from HEK293 co-cultured with PK15; −, negative control, HEK293 cell cultured alone; co indicates HEK-293 cells co-cultured with XENO-1 islets.

    Article Snippet: The PCR products were purified using the Agarose Gel DNA Purification Kit (TaKaRa), inserted into pUCm-T vectors, and transformed into the E.coli strain HT115.

    Techniques: Co-Culture Assay, Infection, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Positive Control, Negative Control

    Transcriptional activity of PERV. Here we demonstrate PERV-A, PERV-B, gag, pol and mtDNA were detected by RT-PCR from PBMC of xeno-1 animals except PERV-C. M, 100 bp DNA ladder; +, positive control; −, negative control; lanes 1–6 indicates different pig samples.

    Journal: Virology Journal

    Article Title: Characterization of PERV in a new conserved pig herd as potential donor animals for xenotransplantation in China

    doi: 10.1186/s12985-014-0212-1

    Figure Lengend Snippet: Transcriptional activity of PERV. Here we demonstrate PERV-A, PERV-B, gag, pol and mtDNA were detected by RT-PCR from PBMC of xeno-1 animals except PERV-C. M, 100 bp DNA ladder; +, positive control; −, negative control; lanes 1–6 indicates different pig samples.

    Article Snippet: The PCR products were purified using the Agarose Gel DNA Purification Kit (TaKaRa), inserted into pUCm-T vectors, and transformed into the E.coli strain HT115.

    Techniques: Activity Assay, Reverse Transcription Polymerase Chain Reaction, Positive Control, Negative Control

    Sensitivity and specificity of PCR for PERV proviral DNA and mtDNA, 100 ng, 50 ng, 10 ng, 1 ng, 100 pg, 10 pg and 0 indicates the varying amount of DNA templates.

    Journal: Virology Journal

    Article Title: Characterization of PERV in a new conserved pig herd as potential donor animals for xenotransplantation in China

    doi: 10.1186/s12985-014-0212-1

    Figure Lengend Snippet: Sensitivity and specificity of PCR for PERV proviral DNA and mtDNA, 100 ng, 50 ng, 10 ng, 1 ng, 100 pg, 10 pg and 0 indicates the varying amount of DNA templates.

    Article Snippet: The PCR products were purified using the Agarose Gel DNA Purification Kit (TaKaRa), inserted into pUCm-T vectors, and transformed into the E.coli strain HT115.

    Techniques: Polymerase Chain Reaction

    Identification of Leptin. a Electrophoresis of full-length target gene RT-PCR product. M: DNA Marker DL10,000. 1: Leptin. b NheI and BamHI digestion and electrophoresis of pIRES2-EGFP-leptin eukaryotic expression vector. M: DNA Marker DL10,000. 1: pIRES2-EGFP-leptin

    Journal: Cytotechnology

    Article Title: Construction of a recombinant eukaryotic expression vector containing a leptin gene and its expression in HPMSCs

    doi: 10.1007/s10616-013-9599-6

    Figure Lengend Snippet: Identification of Leptin. a Electrophoresis of full-length target gene RT-PCR product. M: DNA Marker DL10,000. 1: Leptin. b NheI and BamHI digestion and electrophoresis of pIRES2-EGFP-leptin eukaryotic expression vector. M: DNA Marker DL10,000. 1: pIRES2-EGFP-leptin

    Article Snippet: An RNAiso Plus, High Fidelity Prime Script™ RT-PCR Kit, TaKaRa Agarose Gel DNA Purification Kit Ver.

    Techniques: Electrophoresis, Reverse Transcription Polymerase Chain Reaction, Marker, Expressing, Plasmid Preparation

    MoVps35 interacts with MoAtg8 and is involved in the retrieval of MoAtg8. (A) GFP-trap based pull down experiment indicating the interaction between MoVps35-GFP and RFP-MoAtg8 in the transformant. Total proteins isolated from the wild-type strain (WT) are included as a negative control. MoVps35-GFP shows specific interaction with the cleaved and lipidated MoAtg8 during MM-N induced starvation. Top, middle, and bottom images represent immunoblot detection with anti-RFP, anti-GFP, and anti-actin antibodies, respectively, as indicated. (B) Delayed post-translational processing of MoAtg8 in ΔMovps35 mutant. Immunoblot analysis of total lysates from CM and MM-N cultured (3 h to 9 h in starvation environment) ΔMoatg8 RFP-MoATG8 and ΔMovps35 RFP-MoATG8 transformants, with anti-RFP antibody. Coomassie blue staining of total lysates serves as a loading control.

    Journal: PLoS Genetics

    Article Title: Retromer Is Essential for Autophagy-Dependent Plant Infection by the Rice Blast Fungus

    doi: 10.1371/journal.pgen.1005704

    Figure Lengend Snippet: MoVps35 interacts with MoAtg8 and is involved in the retrieval of MoAtg8. (A) GFP-trap based pull down experiment indicating the interaction between MoVps35-GFP and RFP-MoAtg8 in the transformant. Total proteins isolated from the wild-type strain (WT) are included as a negative control. MoVps35-GFP shows specific interaction with the cleaved and lipidated MoAtg8 during MM-N induced starvation. Top, middle, and bottom images represent immunoblot detection with anti-RFP, anti-GFP, and anti-actin antibodies, respectively, as indicated. (B) Delayed post-translational processing of MoAtg8 in ΔMovps35 mutant. Immunoblot analysis of total lysates from CM and MM-N cultured (3 h to 9 h in starvation environment) ΔMoatg8 RFP-MoATG8 and ΔMovps35 RFP-MoATG8 transformants, with anti-RFP antibody. Coomassie blue staining of total lysates serves as a loading control.

    Article Snippet: Proteins eluted from GFP-Trap agarose beads were analyzed by immunoblot detection with the anti-RFP (Clontech, USA), anti-GFP (Sigma-Aldrich) antibodies and anti-Actin (Sigma-Aldrich).

    Techniques: Isolation, Negative Control, Mutagenesis, Cell Culture, Staining

    Workflow of the SMV-based gene delivery for identification of cellular interacting protein partners. Schematic representation of SMV recombinant constructs shows in-frame insertion of the Flag-tagged GFP and Flag-tagged HC-Pro into the P1/HC-Pro gene insertion cassette of pSMV-Dual vectors. Each SMV recombinant construct was rub-inoculated on the leaves of soybean seedlings. At 15 dpi, crude plant extracts prepared by homogenizing the upper symptomatic leaves were subjected to immunoprecipitation using anti-Flag antibody-conjugated agarose beads. The resulting co-immunoprecipitated products were analyzed by SDS-PAGE and the bands of interest (indicated by asterisks) were excised from the gel and subjected to LC-MS/MS analysis. The identified proteins and MS/MS spectral information are shown.

    Journal: Scientific Reports

    Article Title: Engineering of soybean mosaic virus as a versatile tool for studying protein–protein interactions in soybean

    doi: 10.1038/srep22436

    Figure Lengend Snippet: Workflow of the SMV-based gene delivery for identification of cellular interacting protein partners. Schematic representation of SMV recombinant constructs shows in-frame insertion of the Flag-tagged GFP and Flag-tagged HC-Pro into the P1/HC-Pro gene insertion cassette of pSMV-Dual vectors. Each SMV recombinant construct was rub-inoculated on the leaves of soybean seedlings. At 15 dpi, crude plant extracts prepared by homogenizing the upper symptomatic leaves were subjected to immunoprecipitation using anti-Flag antibody-conjugated agarose beads. The resulting co-immunoprecipitated products were analyzed by SDS-PAGE and the bands of interest (indicated by asterisks) were excised from the gel and subjected to LC-MS/MS analysis. The identified proteins and MS/MS spectral information are shown.

    Article Snippet: The resulting supernatants were incubated with anti-Flag antibody conjugated agarose beads (Takara, Japan) for 16 h at 4 °C.

    Techniques: Recombinant, Construct, Immunoprecipitation, SDS Page, Liquid Chromatography with Mass Spectroscopy, Mass Spectrometry