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Bio-Rad agarose gel
Agarose Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 3055 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SYBR Green Assay:

Article Title: Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods
Article Snippet: .. The electrophoretic run was performed using the DCode System for DGGE by Bio-Rad for 5 h at 200 V. Gel was stained with SYBR Green I (Sigma-Aldrich) and bands were observed with the transilluminator Gel Doc XR (Bio-Rad). .. Bands were cut and purified with sterile water prior the reamplification, which was performed using the same primer pairs, with the addition of an M13 tail to the reverse primer as described in the literature [ ] ( ).

Staining:

Article Title: Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods
Article Snippet: .. The electrophoretic run was performed using the DCode System for DGGE by Bio-Rad for 5 h at 200 V. Gel was stained with SYBR Green I (Sigma-Aldrich) and bands were observed with the transilluminator Gel Doc XR (Bio-Rad). .. Bands were cut and purified with sterile water prior the reamplification, which was performed using the same primer pairs, with the addition of an M13 tail to the reverse primer as described in the literature [ ] ( ).

Incubation:

Article Title: The consequence of NAC on sodium arsenite-induced uterine oxidative stress
Article Snippet: .. The pellet was resuspended in 30 μl of deionized water–RNAase solution along with 5 μl of loading buffer and incubated for 30 min at 37 °C followed by an electrophoretic run under 8.0% agarose gel containing ethidium bromide at 65 V and finally visualized with the Biorad documentation system [ ]. ..

Denaturing Gradient Gel Electrophoresis:

Article Title: Monitoring of Air Microbial Contaminations in Different Bioenergy Facilities Using Cultural and Biomolecular Methods
Article Snippet: .. The electrophoretic run was performed using the DCode System for DGGE by Bio-Rad for 5 h at 200 V. Gel was stained with SYBR Green I (Sigma-Aldrich) and bands were observed with the transilluminator Gel Doc XR (Bio-Rad). .. Bands were cut and purified with sterile water prior the reamplification, which was performed using the same primer pairs, with the addition of an M13 tail to the reverse primer as described in the literature [ ] ( ).

Agarose Gel Electrophoresis:

Article Title: The consequence of NAC on sodium arsenite-induced uterine oxidative stress
Article Snippet: .. The pellet was resuspended in 30 μl of deionized water–RNAase solution along with 5 μl of loading buffer and incubated for 30 min at 37 °C followed by an electrophoretic run under 8.0% agarose gel containing ethidium bromide at 65 V and finally visualized with the Biorad documentation system [ ]. ..

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  • 85
    Bio-Rad pulse field gel electrophoresis pfge agarose embedded dna
    Diagnostic PCR using <t>DNA</t> extracted during purification of the bacterial endosymbiont . A. When PCR was performed using a template prepared from the fat bodies, DNA fragments of B. cuenoti (b), mitochondria (m) and host Panesthia nuclei (h) were observed. B. Although the host DNA was disappeared after the Percoll centrifugation, only a trace of contamination of mitochondrial DNA was amplified. C. When the B. cuenoti DNA was digested with I- Ceu I separated using <t>PFGE,</t> no contaminations of mitochondrial and host's DNAs were detected. D. As PCR cycles were increased to be 35 cycles, no contaminations were detectable. M: 100-bp ladder.
    Pulse Field Gel Electrophoresis Pfge Agarose Embedded Dna, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bio-Rad agarose ethidium bromide gel
    <t>Ethidium</t> bromide/Acridine orange stained colonocytes of animals belonging to different groups: a ) Control; b ) DMH-treated; c ) celecoxib + DMH; d ) L.acidophilus + celecoxib+DMH; e ) L.rhamnosus GG + celecoxib+DMH; f ) L.acidophilus + L.rhamnosus GG + celecoxib+DMH (400X)
    Agarose Ethidium Bromide Gel, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad pulsed field gel electrophoresis pfge certified agarose
    <t>PFGE</t> time course of recovery after exposure to 2.5 kGy of γ radiation. Samples were taken preirradiation (P) and immediately following irradiation (0) and every 4 h during the recovery up to 12 h and were embedded in InCert agarose plugs at a final density of 1 × 10 9 cells/ml. Plugs were digested with XbaI prior to gel electrophoresis. Images were taken from one of three independent replicates of this experiment.
    Pulsed Field Gel Electrophoresis Pfge Certified Agarose, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad deae affi gel blue sepharose column
    Fig. 6. SnRK is associated with SKP1/ASK1, cullin and 20S proteasome α-subunits. ( A ) SKP1/ASK1 is specifically detected by the polyclonal α-ASK1 antibody. Double-staining of Arabidopsis cells expressing an HA epitope-tagged form of SKP1/ASK1 protein (ASK1-HA) with polyclonal α-ASK1 (red) and monoclonal anti-HA (green) antibodies shows identical images of mitotic spindles in late anaphase (left column) and phragmoplasts in telophase (right column). Chromosomes and daughter nuclei are stained with DAPI (blue). Bars = 5 µm. ( B ) SKP1/ASK1 is co-immunoprecipitated with SnRK and cullin. Protein extract prepared from Arabidopsis cells expressing ASK1-HA was bound to immobilized anti-HA.11 IgG. The crude extract (T) and proteins eluted from the α-HA IgG matrix (IP) were separated by SDS–PAGE and immunoblotted with α-HA, α-SnRK and α-AtCUL1 antibodies. A control immunoprecipitation experiment was performed under identical conditions with a protein extract prepared from a cell line expressing an HA epitope-tagged β-glucuronidase enzyme (HA-GUS). ( C ) Purification of 20S proteasome–SCF complex. 20S proteasomal fractions co-purifying with cullin, SnRK and ASK1-HA on <t>DEAE-Affi-Gel</t> blue and Sephacryl S-400 were immunoaffinity purified on an anti-HA.11 IgG column. Proteins eluted with HA-peptide were separated by SDS–PAGE and immunoblotted with α-20S proteasome and α-HA antibodies. ( D ) Immunaffinity binding to α-HA and α-SnRK IgGs destabilizes the SCF complex. A Sephacryl S-400-purified 20S proteasome–SCF fraction (TOTAL) was immunoprecipitated using immobilized α-AtCUL1, α-SnRK and α-HA IgG antibodies, separated by SDS–PAGE and immunoblotted with α-HA, α-AtCUL1 and α-SnRK antibodies.
    Deae Affi Gel Blue Sepharose Column, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Diagnostic PCR using DNA extracted during purification of the bacterial endosymbiont . A. When PCR was performed using a template prepared from the fat bodies, DNA fragments of B. cuenoti (b), mitochondria (m) and host Panesthia nuclei (h) were observed. B. Although the host DNA was disappeared after the Percoll centrifugation, only a trace of contamination of mitochondrial DNA was amplified. C. When the B. cuenoti DNA was digested with I- Ceu I separated using PFGE, no contaminations of mitochondrial and host's DNAs were detected. D. As PCR cycles were increased to be 35 cycles, no contaminations were detectable. M: 100-bp ladder.

    Journal: BMC Research Notes

    Article Title: Purification and partial genome characterization of the bacterial endosymbiont Blattabacterium cuenoti from the fat bodies of cockroaches

    doi: 10.1186/1756-0500-1-118

    Figure Lengend Snippet: Diagnostic PCR using DNA extracted during purification of the bacterial endosymbiont . A. When PCR was performed using a template prepared from the fat bodies, DNA fragments of B. cuenoti (b), mitochondria (m) and host Panesthia nuclei (h) were observed. B. Although the host DNA was disappeared after the Percoll centrifugation, only a trace of contamination of mitochondrial DNA was amplified. C. When the B. cuenoti DNA was digested with I- Ceu I separated using PFGE, no contaminations of mitochondrial and host's DNAs were detected. D. As PCR cycles were increased to be 35 cycles, no contaminations were detectable. M: 100-bp ladder.

    Article Snippet: Pulse-field gel electrophoresis (PFGE) Agarose embedded DNA (plug) was prepared using a CHEF bacterial genomic DNA plug kit (Bio-Rad, Hercules, CA, USA).

    Techniques: Diagnostic Assay, Polymerase Chain Reaction, Purification, Centrifugation, Amplification

    Ethidium bromide/Acridine orange stained colonocytes of animals belonging to different groups: a ) Control; b ) DMH-treated; c ) celecoxib + DMH; d ) L.acidophilus + celecoxib+DMH; e ) L.rhamnosus GG + celecoxib+DMH; f ) L.acidophilus + L.rhamnosus GG + celecoxib+DMH (400X)

    Journal: BMC Cancer

    Article Title: Prophylactic intervention of probiotics (L.acidophilus, L.rhamnosus GG) and celecoxib modulate Bax-mediated apoptosis in 1,2-dimethylhydrazine-induced experimental colon carcinogenesis

    doi: 10.1186/s12885-018-4999-9

    Figure Lengend Snippet: Ethidium bromide/Acridine orange stained colonocytes of animals belonging to different groups: a ) Control; b ) DMH-treated; c ) celecoxib + DMH; d ) L.acidophilus + celecoxib+DMH; e ) L.rhamnosus GG + celecoxib+DMH; f ) L.acidophilus + L.rhamnosus GG + celecoxib+DMH (400X)

    Article Snippet: The amplified DNA was resolved in 1.8% agarose ethidium bromide gel and analysed by Gel Doc EZ Imager (Bio-Rad, USA).

    Techniques: Staining

    PFGE time course of recovery after exposure to 2.5 kGy of γ radiation. Samples were taken preirradiation (P) and immediately following irradiation (0) and every 4 h during the recovery up to 12 h and were embedded in InCert agarose plugs at a final density of 1 × 10 9 cells/ml. Plugs were digested with XbaI prior to gel electrophoresis. Images were taken from one of three independent replicates of this experiment.

    Journal: Journal of Bacteriology

    Article Title: Rad50 Is Not Essential for the Mre11-Dependent Repair of DNA Double-Strand Breaks in Halobacterium sp. Strain NRC-1

    doi: 10.1128/JB.00292-08

    Figure Lengend Snippet: PFGE time course of recovery after exposure to 2.5 kGy of γ radiation. Samples were taken preirradiation (P) and immediately following irradiation (0) and every 4 h during the recovery up to 12 h and were embedded in InCert agarose plugs at a final density of 1 × 10 9 cells/ml. Plugs were digested with XbaI prior to gel electrophoresis. Images were taken from one of three independent replicates of this experiment.

    Article Snippet: Halobacterium genomic DNA plugs were analyzed using a CHEF DR-III electrophoresis system (Bio-Rad, Hercules, CA) with1% pulsed-field gel electrophoresis (PFGE)-certified agarose (Bio-Rad, Hercules, CA) gels and 0.25× Tris-borate-EDTA in both the running and gel buffers.

    Techniques: Irradiation, Nucleic Acid Electrophoresis

    Fig. 6. SnRK is associated with SKP1/ASK1, cullin and 20S proteasome α-subunits. ( A ) SKP1/ASK1 is specifically detected by the polyclonal α-ASK1 antibody. Double-staining of Arabidopsis cells expressing an HA epitope-tagged form of SKP1/ASK1 protein (ASK1-HA) with polyclonal α-ASK1 (red) and monoclonal anti-HA (green) antibodies shows identical images of mitotic spindles in late anaphase (left column) and phragmoplasts in telophase (right column). Chromosomes and daughter nuclei are stained with DAPI (blue). Bars = 5 µm. ( B ) SKP1/ASK1 is co-immunoprecipitated with SnRK and cullin. Protein extract prepared from Arabidopsis cells expressing ASK1-HA was bound to immobilized anti-HA.11 IgG. The crude extract (T) and proteins eluted from the α-HA IgG matrix (IP) were separated by SDS–PAGE and immunoblotted with α-HA, α-SnRK and α-AtCUL1 antibodies. A control immunoprecipitation experiment was performed under identical conditions with a protein extract prepared from a cell line expressing an HA epitope-tagged β-glucuronidase enzyme (HA-GUS). ( C ) Purification of 20S proteasome–SCF complex. 20S proteasomal fractions co-purifying with cullin, SnRK and ASK1-HA on DEAE-Affi-Gel blue and Sephacryl S-400 were immunoaffinity purified on an anti-HA.11 IgG column. Proteins eluted with HA-peptide were separated by SDS–PAGE and immunoblotted with α-20S proteasome and α-HA antibodies. ( D ) Immunaffinity binding to α-HA and α-SnRK IgGs destabilizes the SCF complex. A Sephacryl S-400-purified 20S proteasome–SCF fraction (TOTAL) was immunoprecipitated using immobilized α-AtCUL1, α-SnRK and α-HA IgG antibodies, separated by SDS–PAGE and immunoblotted with α-HA, α-AtCUL1 and α-SnRK antibodies.

    Journal: The EMBO Journal

    Article Title: SKP1-SnRK protein kinase interactions mediate proteasomal binding of a plant SCF ubiquitin ligase

    doi: 10.1093/emboj/20.11.2742

    Figure Lengend Snippet: Fig. 6. SnRK is associated with SKP1/ASK1, cullin and 20S proteasome α-subunits. ( A ) SKP1/ASK1 is specifically detected by the polyclonal α-ASK1 antibody. Double-staining of Arabidopsis cells expressing an HA epitope-tagged form of SKP1/ASK1 protein (ASK1-HA) with polyclonal α-ASK1 (red) and monoclonal anti-HA (green) antibodies shows identical images of mitotic spindles in late anaphase (left column) and phragmoplasts in telophase (right column). Chromosomes and daughter nuclei are stained with DAPI (blue). Bars = 5 µm. ( B ) SKP1/ASK1 is co-immunoprecipitated with SnRK and cullin. Protein extract prepared from Arabidopsis cells expressing ASK1-HA was bound to immobilized anti-HA.11 IgG. The crude extract (T) and proteins eluted from the α-HA IgG matrix (IP) were separated by SDS–PAGE and immunoblotted with α-HA, α-SnRK and α-AtCUL1 antibodies. A control immunoprecipitation experiment was performed under identical conditions with a protein extract prepared from a cell line expressing an HA epitope-tagged β-glucuronidase enzyme (HA-GUS). ( C ) Purification of 20S proteasome–SCF complex. 20S proteasomal fractions co-purifying with cullin, SnRK and ASK1-HA on DEAE-Affi-Gel blue and Sephacryl S-400 were immunoaffinity purified on an anti-HA.11 IgG column. Proteins eluted with HA-peptide were separated by SDS–PAGE and immunoblotted with α-20S proteasome and α-HA antibodies. ( D ) Immunaffinity binding to α-HA and α-SnRK IgGs destabilizes the SCF complex. A Sephacryl S-400-purified 20S proteasome–SCF fraction (TOTAL) was immunoprecipitated using immobilized α-AtCUL1, α-SnRK and α-HA IgG antibodies, separated by SDS–PAGE and immunoblotted with α-HA, α-AtCUL1 and α-SnRK antibodies.

    Article Snippet: Proteasomes were similarly prepared from the ASK1-HA-expressing Arabidopsis cell line, with the exception that the cleared lysate (2 g of protein) was fractionated on a DEAE-Affi-Gel blue Sepharose column (Bio-Rad) using a linear gradient of 0–500 mM NaCl in buffer E [25 mM Tris–HCl pH 7.5, 2 mM ATP, 5 mM MgCl2 , 1 mM DTT, 10% (v/v) glycerol].

    Techniques: Double Staining, Expressing, Staining, Immunoprecipitation, SDS Page, Purification, Binding Assay