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Promega agarose gel pcr fragment clean up system
<t>PCR-SSCP</t> and sequencing of pri-miR-133a (A), pri-miR-29a (B) and pri-miR-27b (C). The boxes in the sequencing trace show the genetic variations. CCC (+/−) in (A) and TAATAATAC (+/−) in (B) appear mixed, with disordered peaks due to the deletion locus. GC in (C) appears as double peaks because the genetic variations occurred in one chromosome, but not the other.
Agarose Gel Pcr Fragment Clean Up System, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/agarose gel pcr fragment clean up system/product/Promega
Average 85 stars, based on 2 article reviews
Price from $9.99 to $1999.99
agarose gel pcr fragment clean up system - by Bioz Stars, 2020-03
85/100 stars

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1) Product Images from "Identification and Analysis of Genetic Variations in Pri-MiRNAs Expressed Specifically or at a High Level in Sheep Skeletal Muscle"

Article Title: Identification and Analysis of Genetic Variations in Pri-MiRNAs Expressed Specifically or at a High Level in Sheep Skeletal Muscle

Journal: PLoS ONE

doi: 10.1371/journal.pone.0117327

PCR-SSCP and sequencing of pri-miR-133a (A), pri-miR-29a (B) and pri-miR-27b (C). The boxes in the sequencing trace show the genetic variations. CCC (+/−) in (A) and TAATAATAC (+/−) in (B) appear mixed, with disordered peaks due to the deletion locus. GC in (C) appears as double peaks because the genetic variations occurred in one chromosome, but not the other.
Figure Legend Snippet: PCR-SSCP and sequencing of pri-miR-133a (A), pri-miR-29a (B) and pri-miR-27b (C). The boxes in the sequencing trace show the genetic variations. CCC (+/−) in (A) and TAATAATAC (+/−) in (B) appear mixed, with disordered peaks due to the deletion locus. GC in (C) appears as double peaks because the genetic variations occurred in one chromosome, but not the other.

Techniques Used: Polymerase Chain Reaction, Sequencing, Countercurrent Chromatography

Related Articles

Polymerase Chain Reaction:

Article Title: Identification and Analysis of Genetic Variations in Pri-MiRNAs Expressed Specifically or at a High Level in Sheep Skeletal Muscle
Article Snippet: .. The PCR products that contained different genetic variations were chosen from the SSCP results, separated on a 1.5% agarose gel, purified with Agarose Gel PCR Fragment Clean-up system (Promega, Madison, WI, USA) and sequenced in both directions using Sanger sequencing (BGI, Beijing, China). .. Plasmid constructs and cell transfections To investigate the effects of the genetic variations on pri-miRNA processing and maturation, a cell assay and qRT-PCR were employed to determine whether the level of mature miRNA was affected by these genetic variations.

Sequencing:

Article Title: Identification and Analysis of Genetic Variations in Pri-MiRNAs Expressed Specifically or at a High Level in Sheep Skeletal Muscle
Article Snippet: .. The PCR products that contained different genetic variations were chosen from the SSCP results, separated on a 1.5% agarose gel, purified with Agarose Gel PCR Fragment Clean-up system (Promega, Madison, WI, USA) and sequenced in both directions using Sanger sequencing (BGI, Beijing, China). .. Plasmid constructs and cell transfections To investigate the effects of the genetic variations on pri-miRNA processing and maturation, a cell assay and qRT-PCR were employed to determine whether the level of mature miRNA was affected by these genetic variations.

Amplification:

Article Title: Identification and Analysis of Genetic Variations in Pri-MiRNAs Expressed Specifically or at a High Level in Sheep Skeletal Muscle
Article Snippet: Genotyping and sequencing One hundred and sixty sheep genomic DNA samples, which represented four different sheep breeds (40 Texel, Suffolk, Altay and Hu sheep), were amplified by PCR, and SSCP was employed to detect the polymorphisms present in each PCR product, in accordance with the following protocol: 2 μl of PCR product was mixed with 8 μl of denaturing sample loading buffer. .. The PCR products that contained different genetic variations were chosen from the SSCP results, separated on a 1.5% agarose gel, purified with Agarose Gel PCR Fragment Clean-up system (Promega, Madison, WI, USA) and sequenced in both directions using Sanger sequencing (BGI, Beijing, China).

Agarose Gel Electrophoresis:

Article Title: Identification and Analysis of Genetic Variations in Pri-MiRNAs Expressed Specifically or at a High Level in Sheep Skeletal Muscle
Article Snippet: .. The PCR products that contained different genetic variations were chosen from the SSCP results, separated on a 1.5% agarose gel, purified with Agarose Gel PCR Fragment Clean-up system (Promega, Madison, WI, USA) and sequenced in both directions using Sanger sequencing (BGI, Beijing, China). .. Plasmid constructs and cell transfections To investigate the effects of the genetic variations on pri-miRNA processing and maturation, a cell assay and qRT-PCR were employed to determine whether the level of mature miRNA was affected by these genetic variations.

Purification:

Article Title: Identification and Analysis of Genetic Variations in Pri-MiRNAs Expressed Specifically or at a High Level in Sheep Skeletal Muscle
Article Snippet: .. The PCR products that contained different genetic variations were chosen from the SSCP results, separated on a 1.5% agarose gel, purified with Agarose Gel PCR Fragment Clean-up system (Promega, Madison, WI, USA) and sequenced in both directions using Sanger sequencing (BGI, Beijing, China). .. Plasmid constructs and cell transfections To investigate the effects of the genetic variations on pri-miRNA processing and maturation, a cell assay and qRT-PCR were employed to determine whether the level of mature miRNA was affected by these genetic variations.

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    Promega agarose gel pcr fragment clean up system
    <t>PCR-SSCP</t> and sequencing of pri-miR-133a (A), pri-miR-29a (B) and pri-miR-27b (C). The boxes in the sequencing trace show the genetic variations. CCC (+/−) in (A) and TAATAATAC (+/−) in (B) appear mixed, with disordered peaks due to the deletion locus. GC in (C) appears as double peaks because the genetic variations occurred in one chromosome, but not the other.
    Agarose Gel Pcr Fragment Clean Up System, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agarose gel pcr fragment clean up system/product/Promega
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    agarose gel pcr fragment clean up system - by Bioz Stars, 2020-03
    85/100 stars
      Buy from Supplier

    99
    Promega agarose gel
    <t>PCR-SSCP</t> and sequencing of pri-miR-133a (A), pri-miR-29a (B) and pri-miR-27b (C). The boxes in the sequencing trace show the genetic variations. CCC (+/−) in (A) and TAATAATAC (+/−) in (B) appear mixed, with disordered peaks due to the deletion locus. GC in (C) appears as double peaks because the genetic variations occurred in one chromosome, but not the other.
    Agarose Gel, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 1365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/agarose gel/product/Promega
    Average 99 stars, based on 1365 article reviews
    Price from $9.99 to $1999.99
    agarose gel - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    99
    Promega dna clean up kit
    <t>PCR-SSCP</t> and sequencing of pri-miR-133a (A), pri-miR-29a (B) and pri-miR-27b (C). The boxes in the sequencing trace show the genetic variations. CCC (+/−) in (A) and TAATAATAC (+/−) in (B) appear mixed, with disordered peaks due to the deletion locus. GC in (C) appears as double peaks because the genetic variations occurred in one chromosome, but not the other.
    Dna Clean Up Kit, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna clean up kit/product/Promega
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    dna clean up kit - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    PCR-SSCP and sequencing of pri-miR-133a (A), pri-miR-29a (B) and pri-miR-27b (C). The boxes in the sequencing trace show the genetic variations. CCC (+/−) in (A) and TAATAATAC (+/−) in (B) appear mixed, with disordered peaks due to the deletion locus. GC in (C) appears as double peaks because the genetic variations occurred in one chromosome, but not the other.

    Journal: PLoS ONE

    Article Title: Identification and Analysis of Genetic Variations in Pri-MiRNAs Expressed Specifically or at a High Level in Sheep Skeletal Muscle

    doi: 10.1371/journal.pone.0117327

    Figure Lengend Snippet: PCR-SSCP and sequencing of pri-miR-133a (A), pri-miR-29a (B) and pri-miR-27b (C). The boxes in the sequencing trace show the genetic variations. CCC (+/−) in (A) and TAATAATAC (+/−) in (B) appear mixed, with disordered peaks due to the deletion locus. GC in (C) appears as double peaks because the genetic variations occurred in one chromosome, but not the other.

    Article Snippet: The PCR products that contained different genetic variations were chosen from the SSCP results, separated on a 1.5% agarose gel, purified with Agarose Gel PCR Fragment Clean-up system (Promega, Madison, WI, USA) and sequenced in both directions using Sanger sequencing (BGI, Beijing, China).

    Techniques: Polymerase Chain Reaction, Sequencing, Countercurrent Chromatography