Journal: BMC Gastroenterology

Article Title: A novel predictive model of microvascular invasion in hepatocellular carcinoma based on differential protein expression

doi: 10.1186/s12876-023-02729-z

Figure Lengend Snippet: Univariate logistic regression analysis of MVI occurrence in the training cohort

Article Snippet: Subsequently, the following primary antibodies were added: GPC3, CK19, vimentin, mutant p53, AFP, EGFR, VEGF (all sourced from Santa Cruz Biotechnology, USA), RRM1, and BRCA1 (both sourced from Abcam, UK), After incubation for 30 min, the cells were stained with ultraView Universal DAB Kit (Roche Diagnostics, GER).

Techniques:

Journal: Stem Cells International

Article Title: An Alternate Approach to Generate Induced Pluripotent Stem Cells with Precise CRISPR/Cas9 Tool

doi: 10.1155/2022/4537335

Figure Lengend Snippet: The differentiation potential of iPSCs. (a) Formation of embryoid bodies (black arrowhead) by the hanging drop method. Scale bar: 100 μ m. (b and c) Immunofluorescence staining of spontaneously differentiating embryoid bodies of HDF-OSK (b) or HEK293T-OSK (c) clones for markers of each germ layer, i.e., α -fetoprotein (AFP) for endoderm, α -smooth muscle actin ( α -SMA) for mesoderm, and β III tubulin (TUJ-1) for ectoderm. Nuclei were stained with a Hoechst 33258 solution, and images were captured by means of a fluorescence microscope (Olympus IX53; Olympus Corporation, Tokyo, Japan). Scale bar: 25 μ m.

Article Snippet: To detect germ layer markers of differentiation, the following primary antibodies were used: anti-AFP (for endoderm; 1 : 100; Santa Cruz Biotechnology), anti-SMA (for mesoderm; 1 : 250, Sigma), and anti-TUJ-1 (for ectoderm; 1 : 250; Abcam).

Techniques: Immunofluorescence, Staining, Clone Assay, Fluorescence, Microscopy

Journal: PLoS ONE

Article Title: Dynamic microRNA Profiles of Hepatic Differentiated Human Umbilical Cord Lining-Derived Mesenchymal Stem Cells

doi: 10.1371/journal.pone.0044737

Figure Lengend Snippet: Hepatocyte-specific gene expression analyzed by qRT-PCR in hUC-MSCs and hepatic differentiated hUC-MSCs for 6 days, 14 days and 26 days. Hepatocyte-specific gene expression was normalized to GAPDH expression, and the results are expressed relative to a value of 1 in the control hUC-MSCs. B: immunofluorescence of hepatocyte-specific gene expression in MSCs and hepatic differentiated MSCs. After induction for 14 days, hUC-MSCs can express AFP, ALB and CK18.

Article Snippet: The antibodies used for immunofluorescence are as follows: human anti-ALB (Santa Cruz Biotechnology, California, USA), human anti-CK18 (Santa Cruz Biotechnology), human anti-AFP (Santa Cruz Biotechnology), and Cy5-conjugated goat anti-human IgG (Jackson Laboratories).

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence