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Santa Cruz Biotechnology anti afp alpha fetoprotein antibody c3
Anti Afp Alpha Fetoprotein Antibody C3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Santa Cruz Biotechnology afp
The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein <t>(AFP)</t> (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype <t>control</t> <t>antibodies</t> were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.
Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/afp/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
afp - by Bioz Stars, 2024-06
86/100 stars

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1) Product Images from "Immortalized hepatocyte-like cells: A competent hepatocyte model for studying clinical HCV isolate infection"

Article Title: Immortalized hepatocyte-like cells: A competent hepatocyte model for studying clinical HCV isolate infection

Journal: PLOS ONE

doi: 10.1371/journal.pone.0303265

The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein (AFP) (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype control antibodies were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.
Figure Legend Snippet: The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein (AFP) (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype control antibodies were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.

Techniques Used: Expressing, Immunofluorescence, Staining, Fluorescence, Imaging


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Santa Cruz Biotechnology afp
Immunophenotyping panel for multiplexed tissue imaging of cancer.
Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/afp/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
afp - by Bioz Stars, 2024-06
86/100 stars

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1) Product Images from "Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging"

Article Title: Unveiling spatial complexity in solid tumor immune microenvironments through multiplexed imaging

Journal: Frontiers in Immunology

doi: 10.3389/fimmu.2024.1383932

Immunophenotyping panel for multiplexed tissue imaging of cancer.
Figure Legend Snippet: Immunophenotyping panel for multiplexed tissue imaging of cancer.

Techniques Used: Imaging


Structured Review

Santa Cruz Biotechnology sc53142 ab 2273670 goat anti afp endodermal
Sc53142 Ab 2273670 Goat Anti Afp Endodermal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sc53142 ab 2273670 goat anti afp endodermal/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
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sc53142 ab 2273670 goat anti afp endodermal - by Bioz Stars, 2024-06
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Santa Cruz Biotechnology afp
Overview of the experimental workflow of fluorescent-nanoparticle tracking analysis (F-NTA) to quantify HCC exosomes in their native state. ( A ). Shows antibody selection to immunocapture HCC exosomes. PE-labeled <t>AFP</t> and <t>GPC3</t> <t>antibodies</t> are specific to HCC-derived exosomes used alone or in combination. ( B ). Brief presentation of clinical assay used to capture highly purified HCC exosomes for F-NTA quantification. 10μL of human serum was diluted in 100μL of PBS with 1% BSA. Antibody incubation was performed overnight at 4°C with shaking. The next day, the sample was diluted in 1mL of ultrapure-filtered distilled water and passed through the Sephadex column. Samples were further diluted 1:10 in ultrapure water and analyzed by NTA in light scattered mode (LSM) and fluorescence mode (FM).
Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/afp/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
afp - by Bioz Stars, 2024-06
86/100 stars

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1) Product Images from "A Single-Step Immunocapture Assay to Quantify HCC Exosomes Using the Highly Sensitive Fluorescence Nanoparticle-Tracking Analysis"

Article Title: A Single-Step Immunocapture Assay to Quantify HCC Exosomes Using the Highly Sensitive Fluorescence Nanoparticle-Tracking Analysis

Journal: Journal of Hepatocellular Carcinoma

doi: 10.2147/JHC.S423043

Overview of the experimental workflow of fluorescent-nanoparticle tracking analysis (F-NTA) to quantify HCC exosomes in their native state. ( A ). Shows antibody selection to immunocapture HCC exosomes. PE-labeled AFP and GPC3 antibodies are specific to HCC-derived exosomes used alone or in combination. ( B ). Brief presentation of clinical assay used to capture highly purified HCC exosomes for F-NTA quantification. 10μL of human serum was diluted in 100μL of PBS with 1% BSA. Antibody incubation was performed overnight at 4°C with shaking. The next day, the sample was diluted in 1mL of ultrapure-filtered distilled water and passed through the Sephadex column. Samples were further diluted 1:10 in ultrapure water and analyzed by NTA in light scattered mode (LSM) and fluorescence mode (FM).
Figure Legend Snippet: Overview of the experimental workflow of fluorescent-nanoparticle tracking analysis (F-NTA) to quantify HCC exosomes in their native state. ( A ). Shows antibody selection to immunocapture HCC exosomes. PE-labeled AFP and GPC3 antibodies are specific to HCC-derived exosomes used alone or in combination. ( B ). Brief presentation of clinical assay used to capture highly purified HCC exosomes for F-NTA quantification. 10μL of human serum was diluted in 100μL of PBS with 1% BSA. Antibody incubation was performed overnight at 4°C with shaking. The next day, the sample was diluted in 1mL of ultrapure-filtered distilled water and passed through the Sephadex column. Samples were further diluted 1:10 in ultrapure water and analyzed by NTA in light scattered mode (LSM) and fluorescence mode (FM).

Techniques Used: Selection, Labeling, Derivative Assay, Purification, Incubation, Fluorescence

Assay calibration using exosome purified from Huh7 culture. Approximately 1×10 7 Huh 7 cells were cultured in a 10-cm tissue culture plate. Media were collected after 72 hours and exosomes were precipitated from 1-mL cell-free supernatants by precipitation. Exosomes were recovered after centrifugation. The exosome pellet was resuspended in 100μL of PBS with 1%BSA and incubated with PE-labeled GPC3, AFP, and CD9 overnight. The next day, samples were diluted in 1 mL water and passed through a column. Extracellular vesicles (EVs) collected from flow-through were diluted in water (1:10) and immediately analyzed by fluorescent-nanoparticle tracking analysis (F-NTA) using light scattered mode (LSM) and fluorescence mode (FM). ( A ). Video images of PE-conjugated CD9 antibody labeled EVs were captured from Huh7 and HuCCA-1 culture and measured by F-NTA in LSM and FM. ( B ). Representative video image of PE-labeled GPC3 antibody-labeled HCC exosomes captured from HuCCA-1 and Huh7 cells and measured by NTA in LSM and FM. ( C ). Total exosomes and HCC exosomes are released per tumor cell. The total exosome release from HuCCA-1 cells is more than from Huh7 cells. HuCCA-1 cells release a minimum number of HCC exosomes as compared to Huh7 cells. Statistical significance levels were presented as ns for non-significant, * for p<0.05, ** for p<0.01.
Figure Legend Snippet: Assay calibration using exosome purified from Huh7 culture. Approximately 1×10 7 Huh 7 cells were cultured in a 10-cm tissue culture plate. Media were collected after 72 hours and exosomes were precipitated from 1-mL cell-free supernatants by precipitation. Exosomes were recovered after centrifugation. The exosome pellet was resuspended in 100μL of PBS with 1%BSA and incubated with PE-labeled GPC3, AFP, and CD9 overnight. The next day, samples were diluted in 1 mL water and passed through a column. Extracellular vesicles (EVs) collected from flow-through were diluted in water (1:10) and immediately analyzed by fluorescent-nanoparticle tracking analysis (F-NTA) using light scattered mode (LSM) and fluorescence mode (FM). ( A ). Video images of PE-conjugated CD9 antibody labeled EVs were captured from Huh7 and HuCCA-1 culture and measured by F-NTA in LSM and FM. ( B ). Representative video image of PE-labeled GPC3 antibody-labeled HCC exosomes captured from HuCCA-1 and Huh7 cells and measured by NTA in LSM and FM. ( C ). Total exosomes and HCC exosomes are released per tumor cell. The total exosome release from HuCCA-1 cells is more than from Huh7 cells. HuCCA-1 cells release a minimum number of HCC exosomes as compared to Huh7 cells. Statistical significance levels were presented as ns for non-significant, * for p<0.05, ** for p<0.01.

Techniques Used: Purification, Cell Culture, Centrifugation, Incubation, Labeling, Fluorescence

Quantification of AFP+ve HCC exosomes using fluorescent-nanoparticle tracking analysis (F-NTA). Ten microliters of serum samples were diluted in 100μL of PBS with 1% BSA. AFP antibody (1:100) incubation was performed at 4°C overnight. The next day samples were diluted in 1mL of ultrapure water and passed through a gel-filtration column. Samples were further diluted (1:10) in 1 mL water and analyzed by NTA. ( A ). Representative video image of extracellular vesicles (EVs) analysis captured in light scattered mode (LSM) and fluorescence mode (FM) of immunocaptured using PE-conjugated AFP antibody. ( B ). Show the size (mean and mode) measured in LSM and FM. ( C ). Particle size distribution of EVs analyzed in LSM and FM. Quantification of immunocaptured EVs using AFP antibody in normal, cirrhosis and HCC serum detected using FM. The particle number obtained in FM mode was normalized with the particle number obtained in LSM for each sample. Statistical significance levels were presented as ns for non-significant, * for p<0.05 ( D ). ROC analysis shows the sensitivity and specificity values of AFP vesicles in normal, cirrhosis, and HCC.
Figure Legend Snippet: Quantification of AFP+ve HCC exosomes using fluorescent-nanoparticle tracking analysis (F-NTA). Ten microliters of serum samples were diluted in 100μL of PBS with 1% BSA. AFP antibody (1:100) incubation was performed at 4°C overnight. The next day samples were diluted in 1mL of ultrapure water and passed through a gel-filtration column. Samples were further diluted (1:10) in 1 mL water and analyzed by NTA. ( A ). Representative video image of extracellular vesicles (EVs) analysis captured in light scattered mode (LSM) and fluorescence mode (FM) of immunocaptured using PE-conjugated AFP antibody. ( B ). Show the size (mean and mode) measured in LSM and FM. ( C ). Particle size distribution of EVs analyzed in LSM and FM. Quantification of immunocaptured EVs using AFP antibody in normal, cirrhosis and HCC serum detected using FM. The particle number obtained in FM mode was normalized with the particle number obtained in LSM for each sample. Statistical significance levels were presented as ns for non-significant, * for p<0.05 ( D ). ROC analysis shows the sensitivity and specificity values of AFP vesicles in normal, cirrhosis, and HCC.

Techniques Used: Incubation, Filtration, Fluorescence

Quantification of AFP and GPC3 double-positive HCC exosome using the combination of two antibodies (AFP and GPC3). ( A ). Representative video image captured during extracellular vesicles (EVs) analysis in light scattered mode (LSM) and fluorescence mode (FM) of immunocaptured using PE-conjugated AFP and GPC3 antibody combination. ( B ). Show the size (mean and mode) of immunocaptured EVs using two antibodies measured in LSM and FM. ( C ) Quantification of immunocaptured EVs using a combination of two antibodies (GPC3/AFP) in normal, cirrhosis and HCC serum detected using FM. The particle number obtained in FM was normalized with the particle number obtained in LSM for each sample. Statistical significance levels were presented as non-significant, * for p<0.05 ( D ). ROC analysis shows the sensitivity and specificity values of HCC exosome quantification using the combination of AFP and GPC3 antibodies in normal, cirrhosis, and HCC.
Figure Legend Snippet: Quantification of AFP and GPC3 double-positive HCC exosome using the combination of two antibodies (AFP and GPC3). ( A ). Representative video image captured during extracellular vesicles (EVs) analysis in light scattered mode (LSM) and fluorescence mode (FM) of immunocaptured using PE-conjugated AFP and GPC3 antibody combination. ( B ). Show the size (mean and mode) of immunocaptured EVs using two antibodies measured in LSM and FM. ( C ) Quantification of immunocaptured EVs using a combination of two antibodies (GPC3/AFP) in normal, cirrhosis and HCC serum detected using FM. The particle number obtained in FM was normalized with the particle number obtained in LSM for each sample. Statistical significance levels were presented as non-significant, * for p<0.05 ( D ). ROC analysis shows the sensitivity and specificity values of HCC exosome quantification using the combination of AFP and GPC3 antibodies in normal, cirrhosis, and HCC.

Techniques Used: Fluorescence

Performance of magnetic bead assay for HCC detection using antibodies to GPC3 alone, AFP alone, and a combination. ( A ). Illustrate the assay design. Streptavidin-conjugated magnetic beads were attached to a biotinylated CD63 antibody. The next day, beads were incubated with 10μL of the serum diluted in 100μL PBS with 1%BSA overnight. The next day, beads were washed twice and then incubated with PE-conjugated antibodies (GPC3 alone, AFP alone, and combination) for one hour. Following this step, beads were washed and analyzed by a flow cytometer. ( B ). Percentage of exosomes reacted with GPC3 antibody by magnetic bead-based flow assay in normal healthy serum, cirrhosis, and cirrhosis with HCC. ( C ). Percentage of HCC exosome captured using AFP antibody using magnetic bead-based flow assay between normal healthy serum, cirrhosis, and cirrhosis with HCC. ( D ). Percentage of HCC exosome captured using a combination of AFP and GPC3 antibodies in normal healthy serum, cirrhosis, and cirrhosis with HCC. Statistical significance levels were presented as ns for non-significant, ** for p<0.01, *** for p<0.001 ( E ). Representative flow cytometry image showing very high reactivity of HCC exosomes using the combination of two antibodies. ( F ). ROC analysis shows the sensitivity and specificity of immunomagnetic bead flow assay for HCC exosome quantification.
Figure Legend Snippet: Performance of magnetic bead assay for HCC detection using antibodies to GPC3 alone, AFP alone, and a combination. ( A ). Illustrate the assay design. Streptavidin-conjugated magnetic beads were attached to a biotinylated CD63 antibody. The next day, beads were incubated with 10μL of the serum diluted in 100μL PBS with 1%BSA overnight. The next day, beads were washed twice and then incubated with PE-conjugated antibodies (GPC3 alone, AFP alone, and combination) for one hour. Following this step, beads were washed and analyzed by a flow cytometer. ( B ). Percentage of exosomes reacted with GPC3 antibody by magnetic bead-based flow assay in normal healthy serum, cirrhosis, and cirrhosis with HCC. ( C ). Percentage of HCC exosome captured using AFP antibody using magnetic bead-based flow assay between normal healthy serum, cirrhosis, and cirrhosis with HCC. ( D ). Percentage of HCC exosome captured using a combination of AFP and GPC3 antibodies in normal healthy serum, cirrhosis, and cirrhosis with HCC. Statistical significance levels were presented as ns for non-significant, ** for p<0.01, *** for p<0.001 ( E ). Representative flow cytometry image showing very high reactivity of HCC exosomes using the combination of two antibodies. ( F ). ROC analysis shows the sensitivity and specificity of immunomagnetic bead flow assay for HCC exosome quantification.

Techniques Used: Magnetic Beads, Incubation, Flow Cytometry

Comparison of MRI Negative and Positive Groups for Demographics, F-NTA, and Serum  AFP  Results
Figure Legend Snippet: Comparison of MRI Negative and Positive Groups for Demographics, F-NTA, and Serum AFP Results

Techniques Used: Comparison

Statistical analysis determines the performance of HCC exosome quantification by fluorescent-nanoparticle tracking analysis (F-NTA) among MRI positive and negative serum samples of our patient cohort. The fluorescence-positive exosome number is normalized with the total number of extracellular vesicles (EVs) captured by light scatter mode in each sample. ( A ). Quantification of GPC3+ve exosomes in serums obtained from MRI positive and negative cases. ( B ). Quantification of HCC exosome using PE-labeled AFP antibodies in the same set of serum samples. ( C ). Quantification of HCC exosomes using a combination of PE-labeled GPC3 and PE-labeled AFP antibodies. ( D ). Comparison of MRI-positive and MRI-negative cirrhosis samples for serum AFP levels. ( E ). Comparison for GPC3+ve HCC-exosome concentration in AFP negative and AFP positive groups. The upper limit of AFP was considered as 20 ng/mL ( F ). Statistical significance levels were presented as ns for non-significant, * for p<0.05, ** for p<0.01, *** for p<0.001. The exosome size is comparable between the MRI-positive and negative groups. ( G ). Show correlation between HCC-exosome quantification with HCC size determined by MRI in the HCC cohort. ( H ). ROC analysis shows the sensitivity and specificity values of marker-positive exosome quantification and serum AFP levels for diagnosing HCC in liver cirrhosis.
Figure Legend Snippet: Statistical analysis determines the performance of HCC exosome quantification by fluorescent-nanoparticle tracking analysis (F-NTA) among MRI positive and negative serum samples of our patient cohort. The fluorescence-positive exosome number is normalized with the total number of extracellular vesicles (EVs) captured by light scatter mode in each sample. ( A ). Quantification of GPC3+ve exosomes in serums obtained from MRI positive and negative cases. ( B ). Quantification of HCC exosome using PE-labeled AFP antibodies in the same set of serum samples. ( C ). Quantification of HCC exosomes using a combination of PE-labeled GPC3 and PE-labeled AFP antibodies. ( D ). Comparison of MRI-positive and MRI-negative cirrhosis samples for serum AFP levels. ( E ). Comparison for GPC3+ve HCC-exosome concentration in AFP negative and AFP positive groups. The upper limit of AFP was considered as 20 ng/mL ( F ). Statistical significance levels were presented as ns for non-significant, * for p<0.05, ** for p<0.01, *** for p<0.001. The exosome size is comparable between the MRI-positive and negative groups. ( G ). Show correlation between HCC-exosome quantification with HCC size determined by MRI in the HCC cohort. ( H ). ROC analysis shows the sensitivity and specificity values of marker-positive exosome quantification and serum AFP levels for diagnosing HCC in liver cirrhosis.

Techniques Used: Fluorescence, Labeling, Comparison, Concentration Assay, Marker


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Santa Cruz Biotechnology mouse anti afp
Mouse Anti Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti afp/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti afp - by Bioz Stars, 2024-06
86/100 stars

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Santa Cruz Biotechnology mouse monoclonal c3 anti afp
Mouse Monoclonal C3 Anti Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal c3 anti afp/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse monoclonal c3 anti afp - by Bioz Stars, 2024-06
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Santa Cruz Biotechnology mouse monoclonal c3 anti afp
Mouse Monoclonal C3 Anti Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal c3 anti afp/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse monoclonal c3 anti afp - by Bioz Stars, 2024-06
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Santa Cruz Biotechnology mouse anti afp
Induction of primary mouse liver cancer by CRISPR/Cas9-mediated somatic mutagenesis in mice. A 34 TSGs and the Setd5 control site targeted by multiplexed CRISPR/Cas9. Each TSG is indicated with its related cancer signaling pathway. Hepatic delivery of Sp Cas9 and sgRNA expression plasmids is achieved by hydrodynamic tail vein injection (HTVI). B Effect of sgRNA dosage on CRISPR/Cas9-induced liver tumor formation. Each dot indicates one mouse. C Mouse liver specimen with or without tumor nodules (left) and H&E staining of a tumor nodule (right). D Microscopic IHC images of CRISPR/Cas9-induced liver tumors. T#1, T#2, and T#3 represent tumor nodules from three mice. <t>AFP</t> and GP73: HCC <t>biomarkers;</t> <t>CK19:</t> ICC biomarker. E Histology and IHC staining of a liver tumor nodule indicating three heterogeneous regions. Left: these three regions indicated with R1, R2, and R3 in H&E staining; Right: microscopic IHC images for these three regions
Mouse Anti Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti afp/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
mouse anti afp - by Bioz Stars, 2024-06
86/100 stars

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1) Product Images from "Small extrachromosomal circular DNA harboring targeted tumor suppressor gene mutations supports intratumor heterogeneity in mouse liver cancer induced by multiplexed CRISPR/Cas9"

Article Title: Small extrachromosomal circular DNA harboring targeted tumor suppressor gene mutations supports intratumor heterogeneity in mouse liver cancer induced by multiplexed CRISPR/Cas9

Journal: Genome Medicine

doi: 10.1186/s13073-023-01230-2

Induction of primary mouse liver cancer by CRISPR/Cas9-mediated somatic mutagenesis in mice. A 34 TSGs and the Setd5 control site targeted by multiplexed CRISPR/Cas9. Each TSG is indicated with its related cancer signaling pathway. Hepatic delivery of Sp Cas9 and sgRNA expression plasmids is achieved by hydrodynamic tail vein injection (HTVI). B Effect of sgRNA dosage on CRISPR/Cas9-induced liver tumor formation. Each dot indicates one mouse. C Mouse liver specimen with or without tumor nodules (left) and H&E staining of a tumor nodule (right). D Microscopic IHC images of CRISPR/Cas9-induced liver tumors. T#1, T#2, and T#3 represent tumor nodules from three mice. AFP and GP73: HCC biomarkers; CK19: ICC biomarker. E Histology and IHC staining of a liver tumor nodule indicating three heterogeneous regions. Left: these three regions indicated with R1, R2, and R3 in H&E staining; Right: microscopic IHC images for these three regions
Figure Legend Snippet: Induction of primary mouse liver cancer by CRISPR/Cas9-mediated somatic mutagenesis in mice. A 34 TSGs and the Setd5 control site targeted by multiplexed CRISPR/Cas9. Each TSG is indicated with its related cancer signaling pathway. Hepatic delivery of Sp Cas9 and sgRNA expression plasmids is achieved by hydrodynamic tail vein injection (HTVI). B Effect of sgRNA dosage on CRISPR/Cas9-induced liver tumor formation. Each dot indicates one mouse. C Mouse liver specimen with or without tumor nodules (left) and H&E staining of a tumor nodule (right). D Microscopic IHC images of CRISPR/Cas9-induced liver tumors. T#1, T#2, and T#3 represent tumor nodules from three mice. AFP and GP73: HCC biomarkers; CK19: ICC biomarker. E Histology and IHC staining of a liver tumor nodule indicating three heterogeneous regions. Left: these three regions indicated with R1, R2, and R3 in H&E staining; Right: microscopic IHC images for these three regions

Techniques Used: CRISPR, Mutagenesis, Expressing, Injection, Staining, Biomarker Assay, Immunohistochemistry


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Santa Cruz Biotechnology anti afp
Anti Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti afp/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
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    Santa Cruz Biotechnology anti afp alpha fetoprotein antibody c3
    Anti Afp Alpha Fetoprotein Antibody C3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti afp alpha fetoprotein antibody c3/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti afp alpha fetoprotein antibody c3 - by Bioz Stars, 2024-06
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    Santa Cruz Biotechnology afp
    The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein <t>(AFP)</t> (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype <t>control</t> <t>antibodies</t> were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.
    Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/afp/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    afp - by Bioz Stars, 2024-06
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    Santa Cruz Biotechnology sc53142 ab 2273670 goat anti afp endodermal
    The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein <t>(AFP)</t> (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype <t>control</t> <t>antibodies</t> were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.
    Sc53142 Ab 2273670 Goat Anti Afp Endodermal, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sc53142 ab 2273670 goat anti afp endodermal/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
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    sc53142 ab 2273670 goat anti afp endodermal - by Bioz Stars, 2024-06
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    Santa Cruz Biotechnology mouse anti afp
    The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein <t>(AFP)</t> (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype <t>control</t> <t>antibodies</t> were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.
    Mouse Anti Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Santa Cruz Biotechnology mouse monoclonal c3 anti afp
    The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein <t>(AFP)</t> (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype <t>control</t> <t>antibodies</t> were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.
    Mouse Monoclonal C3 Anti Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse monoclonal c3 anti afp/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mouse monoclonal c3 anti afp - by Bioz Stars, 2024-06
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    86
    Santa Cruz Biotechnology anti afp
    The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein <t>(AFP)</t> (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype <t>control</t> <t>antibodies</t> were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.
    Anti Afp, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti afp/product/Santa Cruz Biotechnology
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti afp - by Bioz Stars, 2024-06
    86/100 stars
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    Image Search Results


    The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein (AFP) (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype control antibodies were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.

    Journal: PLOS ONE

    Article Title: Immortalized hepatocyte-like cells: A competent hepatocyte model for studying clinical HCV isolate infection

    doi: 10.1371/journal.pone.0303265

    Figure Lengend Snippet: The attached imHC at confluence exhibited hepatocyte morphology: polygonal shape, granulated cytoplasm and large nucleus (A). The basal expression of hepatocyte markers in human total liver RNA, HepG2, imHC, and Huh7 were analyzed using real-time qPCR (B). Liver proteins, albumin (ALB) (C), α-fetoprotein (AFP) (D), hepatocyte nuclear factor-4-alpha (HNF-4ɑ) (E), Na + -taurocholate cotransporting polypeptide (NTCP) (F) and multidrug resistance-associated protein 2 (MRP2) (G) were evaluated using immunofluorescence. The nuclei were stained with Hoechst 33342. The isotype control antibodies were used as negative controls (H). Fluorescence images were taken by an Operetta High-Content Imaging System (PerkinElmer) with a ×40 objective lens. Scale bar = 50 μm. ND represented “not detected”, whereas a, b, c, and d represented statistical differences between cell lines with a p -value less than 0.05, 0.01, 0.001, and 0.0001 respectively.

    Article Snippet: Primary antibodies against hepatocyte markers were ALB (1:100 dilution, ab10241, Abcam), AFP (1:100 dilution, SC8399, Santa Cruz Biotechnology), Na + -taurocholate cotransporting polypeptide (NTCP;1:100 dilution, ab131084, Abcam), MRP2 (1:100 dilution, ab3373, Abcam) and HNF-4ɑ (1:100 dilution, SC6556, Santa Cruz Biotechnology).

    Techniques: Expressing, Immunofluorescence, Staining, Fluorescence, Imaging