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Obio Technology Corp Ltd shrna expressing vector targeting mouse afp
Shrna Expressing Vector Targeting Mouse Afp, supplied by Obio Technology Corp Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ribobio co afp siafp
Afp Siafp, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ribobio co afp sirna kit
Afp Sirna Kit, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Ribobio co afp sirnas
Axin 1 overexpression reduced <t>AFP-mediated</t> Wnt-pathway activation and malignancy in established APGC cells. Notes: ( A – D ) After Axin 1 knockdown using <t>siRNAs</t> in GC cells and ( E – H ) Axin 1 overexpression in AFP-overexpressing GC cells for 48 hours, Wnt-signaling-involved protein-expression levels, β-catenin-mediated TCF transcriptional activity, and cell-proliferation, -invasion, and -migration abilities were determined by immunoblotting, dual-luciferase, CCK8, and transwell assays, respectively. Data expressed as mean ± SD. * P <0.05 by ANOVA. Abbreviation: APGC, AFP-producing gastric cancer.
Afp Sirnas, supplied by Ribobio co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Activated Wnt signaling promotes growth and progression of AFP-producing gastric cancer in preclinical models"

Article Title: Activated Wnt signaling promotes growth and progression of AFP-producing gastric cancer in preclinical models

Journal: Cancer Management and Research

doi: 10.2147/CMAR.S187219

Axin 1 overexpression reduced AFP-mediated Wnt-pathway activation and malignancy in established APGC cells. Notes: ( A – D ) After Axin 1 knockdown using siRNAs in GC cells and ( E – H ) Axin 1 overexpression in AFP-overexpressing GC cells for 48 hours, Wnt-signaling-involved protein-expression levels, β-catenin-mediated TCF transcriptional activity, and cell-proliferation, -invasion, and -migration abilities were determined by immunoblotting, dual-luciferase, CCK8, and transwell assays, respectively. Data expressed as mean ± SD. * P <0.05 by ANOVA. Abbreviation: APGC, AFP-producing gastric cancer.
Figure Legend Snippet: Axin 1 overexpression reduced AFP-mediated Wnt-pathway activation and malignancy in established APGC cells. Notes: ( A – D ) After Axin 1 knockdown using siRNAs in GC cells and ( E – H ) Axin 1 overexpression in AFP-overexpressing GC cells for 48 hours, Wnt-signaling-involved protein-expression levels, β-catenin-mediated TCF transcriptional activity, and cell-proliferation, -invasion, and -migration abilities were determined by immunoblotting, dual-luciferase, CCK8, and transwell assays, respectively. Data expressed as mean ± SD. * P <0.05 by ANOVA. Abbreviation: APGC, AFP-producing gastric cancer.

Techniques Used: Over Expression, Activation Assay, Expressing, Activity Assay, Migration, Western Blot, Luciferase

afp sirna  (Thermo Fisher)


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    Structured Review

    Thermo Fisher afp sirna
    A, Human liver cancer <t>PLC/PRF/5(AFP-producing)</t> cell line was transfected with <t>AFP-siRNA</t> vectors for 48 hours and CXCR4 expression was observed by laser confocal microscopy. B, human liver HLE(non-AFP-producing) cell lines were transfected with pcDNA3.1- afp vector for 48 hours and CXCR4 expression was observed by laser confocal microscopy. The images were taken of three independent experiments.
    Afp Sirna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/afp sirna/product/Thermo Fisher
    Average 86 stars, based on 1 article reviews
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    afp sirna - by Bioz Stars, 2024-07
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    1) Product Images from "Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells"

    Article Title: Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells

    Journal: Oncoscience

    doi:

    A, Human liver cancer PLC/PRF/5(AFP-producing) cell line was transfected with AFP-siRNA vectors for 48 hours and CXCR4 expression was observed by laser confocal microscopy. B, human liver HLE(non-AFP-producing) cell lines were transfected with pcDNA3.1- afp vector for 48 hours and CXCR4 expression was observed by laser confocal microscopy. The images were taken of three independent experiments.
    Figure Legend Snippet: A, Human liver cancer PLC/PRF/5(AFP-producing) cell line was transfected with AFP-siRNA vectors for 48 hours and CXCR4 expression was observed by laser confocal microscopy. B, human liver HLE(non-AFP-producing) cell lines were transfected with pcDNA3.1- afp vector for 48 hours and CXCR4 expression was observed by laser confocal microscopy. The images were taken of three independent experiments.

    Techniques Used: Transfection, Expressing, Confocal Microscopy, Plasmid Preparation

    A, Expressions of AFP, CXCR4, and PTEN in PLC/PRF/5 or HLE cells were analyzed by Western blotting. B, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours and the expressions of AFP, p-AKT(Ser473), and CXCR4 were analyzed by Western blotting, low column graph indicated the quantity of protein expression. C, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours and the expressions of AFP, p-AKT(Ser473), and CXCR4 in HLE cells were analyzed by Western blotting, low column graph indicated the quantity of protein expression. We performed three separate experiments in triplicate.
    Figure Legend Snippet: A, Expressions of AFP, CXCR4, and PTEN in PLC/PRF/5 or HLE cells were analyzed by Western blotting. B, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours and the expressions of AFP, p-AKT(Ser473), and CXCR4 were analyzed by Western blotting, low column graph indicated the quantity of protein expression. C, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours and the expressions of AFP, p-AKT(Ser473), and CXCR4 in HLE cells were analyzed by Western blotting, low column graph indicated the quantity of protein expression. We performed three separate experiments in triplicate.

    Techniques Used: Western Blot, Transfection, Expressing

    A, Co-location of AFP and PTEN in PLC/PRF/5 or HLE cells were observed by laser confocal microscopy. B, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours and the interaction of AFP with PTEN was detected by co-immunoprecipitation(Co-IP). C, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours and the interaction of AFP with PTEN was detected by Co-IP. We performed separate experiments in triplicate.
    Figure Legend Snippet: A, Co-location of AFP and PTEN in PLC/PRF/5 or HLE cells were observed by laser confocal microscopy. B, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours and the interaction of AFP with PTEN was detected by co-immunoprecipitation(Co-IP). C, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours and the interaction of AFP with PTEN was detected by Co-IP. We performed separate experiments in triplicate.

    Techniques Used: Confocal Microscopy, Transfection, Immunoprecipitation, Co-Immunoprecipitation Assay

    A, PLC/PRF/5 cells were treated with a potent inhibitor of PI3K/AKT, Ly294002 (20μmol/L), or transfected with AFP-siRNA for 48 hours, and the expressions of p-mTOR(Ser2448) and CXCR4 were analyzed by Western blotting, right column graph indicated the quantity of protein expression, * P <0.01 vs non-treated(control) group and scramble-siRNA vectors treated group; # P <0.01 vs control group and scramble-siRNA vectors treated group. B, HLE cells were treated with Ly294002 (20μmol/L) or pcDNA3.1- afp vectors or co-treated with Ly294002 (20μg/mL) and pcDNA3.1- afp for 48 hours, and the expressions of p-mTOR(Ser2448) and CXCR4 were analyzed by Western blotting, right column graph indicated the quantity of protein expression, * P <0.01 vs control group and pcDNA3.1 empty vectors treated group; # P <0.01 vs control group, Ly294002 treated group, pcDNA3.1 empty vectors treated group, and co-treated with Ly294002 (20 μg/mL) and pcDNA3.1- afp group. We performed separate experiments in triplicate.
    Figure Legend Snippet: A, PLC/PRF/5 cells were treated with a potent inhibitor of PI3K/AKT, Ly294002 (20μmol/L), or transfected with AFP-siRNA for 48 hours, and the expressions of p-mTOR(Ser2448) and CXCR4 were analyzed by Western blotting, right column graph indicated the quantity of protein expression, * P <0.01 vs non-treated(control) group and scramble-siRNA vectors treated group; # P <0.01 vs control group and scramble-siRNA vectors treated group. B, HLE cells were treated with Ly294002 (20μmol/L) or pcDNA3.1- afp vectors or co-treated with Ly294002 (20μg/mL) and pcDNA3.1- afp for 48 hours, and the expressions of p-mTOR(Ser2448) and CXCR4 were analyzed by Western blotting, right column graph indicated the quantity of protein expression, * P <0.01 vs control group and pcDNA3.1 empty vectors treated group; # P <0.01 vs control group, Ly294002 treated group, pcDNA3.1 empty vectors treated group, and co-treated with Ly294002 (20 μg/mL) and pcDNA3.1- afp group. We performed separate experiments in triplicate.

    Techniques Used: Transfection, Western Blot, Expressing

    A, PLC/PRF/5 cells were transfected with AFP-siRNA for 48 hours, and the combination of p-mTOR(Ser2448) and the CXCR4 gene promoter was evidenced by chromatin immunoprecipitation(ChIP). B, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours, and the combination of p-mTOR(Ser2448) with the CXCR4 gene promoter was evidenced by ChIP. We performed separate experiments in triplicate.
    Figure Legend Snippet: A, PLC/PRF/5 cells were transfected with AFP-siRNA for 48 hours, and the combination of p-mTOR(Ser2448) and the CXCR4 gene promoter was evidenced by chromatin immunoprecipitation(ChIP). B, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours, and the combination of p-mTOR(Ser2448) with the CXCR4 gene promoter was evidenced by ChIP. We performed separate experiments in triplicate.

    Techniques Used: Transfection, Chromatin Immunoprecipitation

    A, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours, wound healing were observed by microscopy, and migratory cells were analyzed by a transwell chamber assay. B, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours, wound healing of the cells were observed by microscopy, and migratory cells were analyzed by a transwell chamber assay. ** P <0.01. We performed three independent experiments.
    Figure Legend Snippet: A, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours, wound healing were observed by microscopy, and migratory cells were analyzed by a transwell chamber assay. B, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours, wound healing of the cells were observed by microscopy, and migratory cells were analyzed by a transwell chamber assay. ** P <0.01. We performed three independent experiments.

    Techniques Used: Transfection, Microscopy, Transwell Chamber Assay


    Structured Review

    Promega afp sirna nps
    Afp Sirna Nps, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86/100 stars

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    Structured Review

    Promega afp sirna nps
    Afp Sirna Nps, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/afp sirna nps/product/Promega
    Average 86 stars, based on 1 article reviews
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    Promega afp sirna nps
    Evaluation of the effect of using antiangiogenetic drugs and/or <t>AFP</t> <t>-siRNA</t> on HepG2 cell viability. ( a ) Percentage of cell viability of HepG2 exposed to different concentrations of sorafenib and sunitinib for 24 h. ( b ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA for 48 h. ( c ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sorafenib. ( d ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sunitinib. Control (untreated cells) and Scr-siRNA were used as control. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Afp Sirna Nps, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/afp sirna nps/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    afp sirna nps - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Study of siRNA Delivery via Polymeric Nanoparticles in Combination with Angiogenesis Inhibitor for The Treatment of AFP -Related Liver Cancer"

    Article Title: Study of siRNA Delivery via Polymeric Nanoparticles in Combination with Angiogenesis Inhibitor for The Treatment of AFP -Related Liver Cancer

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232012666

    Evaluation of the effect of using antiangiogenetic drugs and/or AFP -siRNA on HepG2 cell viability. ( a ) Percentage of cell viability of HepG2 exposed to different concentrations of sorafenib and sunitinib for 24 h. ( b ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA for 48 h. ( c ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sorafenib. ( d ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sunitinib. Control (untreated cells) and Scr-siRNA were used as control. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Figure Legend Snippet: Evaluation of the effect of using antiangiogenetic drugs and/or AFP -siRNA on HepG2 cell viability. ( a ) Percentage of cell viability of HepG2 exposed to different concentrations of sorafenib and sunitinib for 24 h. ( b ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA for 48 h. ( c ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sorafenib. ( d ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sunitinib. Control (untreated cells) and Scr-siRNA were used as control. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).

    Techniques Used:

    Characteristics of synthesized NPs including unloaded and loaded NPs. ( a ) Hydrodynamic size and zeta potential of unloaded NPs, Scr-siRNA NPs, AFP -siRNA NPs, and BLOCK-iT NPs. ( b ) Releasing profile of loaded siRNA over a 14-day period. BLOCK-iT siRNA was used as a representative of AFP -siRNA. ( c ) Percentage of cell viability of cancer and normal fibroblast cell lines (i.e., HepG2 and HDFa, respectively) after treatment with different concentrations of unloaded NPs for 24 h. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Figure Legend Snippet: Characteristics of synthesized NPs including unloaded and loaded NPs. ( a ) Hydrodynamic size and zeta potential of unloaded NPs, Scr-siRNA NPs, AFP -siRNA NPs, and BLOCK-iT NPs. ( b ) Releasing profile of loaded siRNA over a 14-day period. BLOCK-iT siRNA was used as a representative of AFP -siRNA. ( c ) Percentage of cell viability of cancer and normal fibroblast cell lines (i.e., HepG2 and HDFa, respectively) after treatment with different concentrations of unloaded NPs for 24 h. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).

    Techniques Used: Synthesized, Blocking Assay

    Cellular internalization of siRNA NPs was investigated. ( a ) Histogram analyzed by flow cytometry demonstrated cellular internalized NPs at 1 h. ( b ) Mean Fluorescence Intensity of internalized NPs at different concentrations for 1 h. ( c ) Different concentrations of BLOCK-iT NPs were treated with HepG2 cells at different incubation times. ( d ) The percentage of HepG2 cell viability at different concentrations of AFP -siRNA NPs for 48 h. Untreated HepG2 cell, 2000 µg/mL of unloaded NPs, and Scr-siRNA NPs were used as controls. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Figure Legend Snippet: Cellular internalization of siRNA NPs was investigated. ( a ) Histogram analyzed by flow cytometry demonstrated cellular internalized NPs at 1 h. ( b ) Mean Fluorescence Intensity of internalized NPs at different concentrations for 1 h. ( c ) Different concentrations of BLOCK-iT NPs were treated with HepG2 cells at different incubation times. ( d ) The percentage of HepG2 cell viability at different concentrations of AFP -siRNA NPs for 48 h. Untreated HepG2 cell, 2000 µg/mL of unloaded NPs, and Scr-siRNA NPs were used as controls. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).

    Techniques Used: Flow Cytometry, Fluorescence, Blocking Assay, Incubation

    Antiproliferation and gene silencing effects of sorafenib or sunitinib in combination with AFP -siRNA loaded NPs on HepG2 cell line. ( a ) Percentage of cell viability of the cells. ( b ) Caspase 3/7 activity of the cells when treated with either single or combined treatments of AFP -siRNA NP and drugs. ( c ) Gene silencing effect of combined treatments of siRNA and drugs compared to the single use of AFP -siRNA NPs alone. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Figure Legend Snippet: Antiproliferation and gene silencing effects of sorafenib or sunitinib in combination with AFP -siRNA loaded NPs on HepG2 cell line. ( a ) Percentage of cell viability of the cells. ( b ) Caspase 3/7 activity of the cells when treated with either single or combined treatments of AFP -siRNA NP and drugs. ( c ) Gene silencing effect of combined treatments of siRNA and drugs compared to the single use of AFP -siRNA NPs alone. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).

    Techniques Used: Activity Assay


    Structured Review

    Promega afp sirna nps
    Evaluation of the effect of using antiangiogenetic drugs and/or <t>AFP</t> <t>-siRNA</t> on HepG2 cell viability. ( a ) Percentage of cell viability of HepG2 exposed to different concentrations of sorafenib and sunitinib for 24 h. ( b ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA for 48 h. ( c ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sorafenib. ( d ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sunitinib. Control (untreated cells) and Scr-siRNA were used as control. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Afp Sirna Nps, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/afp sirna nps/product/Promega
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    afp sirna nps - by Bioz Stars, 2024-07
    86/100 stars

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    1) Product Images from "Study of siRNA Delivery via Polymeric Nanoparticles in Combination with Angiogenesis Inhibitor for The Treatment of AFP -Related Liver Cancer"

    Article Title: Study of siRNA Delivery via Polymeric Nanoparticles in Combination with Angiogenesis Inhibitor for The Treatment of AFP -Related Liver Cancer

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms232012666

    Evaluation of the effect of using antiangiogenetic drugs and/or AFP -siRNA on HepG2 cell viability. ( a ) Percentage of cell viability of HepG2 exposed to different concentrations of sorafenib and sunitinib for 24 h. ( b ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA for 48 h. ( c ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sorafenib. ( d ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sunitinib. Control (untreated cells) and Scr-siRNA were used as control. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Figure Legend Snippet: Evaluation of the effect of using antiangiogenetic drugs and/or AFP -siRNA on HepG2 cell viability. ( a ) Percentage of cell viability of HepG2 exposed to different concentrations of sorafenib and sunitinib for 24 h. ( b ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA for 48 h. ( c ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sorafenib. ( d ) Percentage of cell viability of HepG2 exposed to different concentrations of AFP -siRNA followed by sunitinib. Control (untreated cells) and Scr-siRNA were used as control. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).

    Techniques Used:

    Characteristics of synthesized NPs including unloaded and loaded NPs. ( a ) Hydrodynamic size and zeta potential of unloaded NPs, Scr-siRNA NPs, AFP -siRNA NPs, and BLOCK-iT NPs. ( b ) Releasing profile of loaded siRNA over a 14-day period. BLOCK-iT siRNA was used as a representative of AFP -siRNA. ( c ) Percentage of cell viability of cancer and normal fibroblast cell lines (i.e., HepG2 and HDFa, respectively) after treatment with different concentrations of unloaded NPs for 24 h. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Figure Legend Snippet: Characteristics of synthesized NPs including unloaded and loaded NPs. ( a ) Hydrodynamic size and zeta potential of unloaded NPs, Scr-siRNA NPs, AFP -siRNA NPs, and BLOCK-iT NPs. ( b ) Releasing profile of loaded siRNA over a 14-day period. BLOCK-iT siRNA was used as a representative of AFP -siRNA. ( c ) Percentage of cell viability of cancer and normal fibroblast cell lines (i.e., HepG2 and HDFa, respectively) after treatment with different concentrations of unloaded NPs for 24 h. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).

    Techniques Used: Synthesized, Blocking Assay

    Cellular internalization of siRNA NPs was investigated. ( a ) Histogram analyzed by flow cytometry demonstrated cellular internalized NPs at 1 h. ( b ) Mean Fluorescence Intensity of internalized NPs at different concentrations for 1 h. ( c ) Different concentrations of BLOCK-iT NPs were treated with HepG2 cells at different incubation times. ( d ) The percentage of HepG2 cell viability at different concentrations of AFP -siRNA NPs for 48 h. Untreated HepG2 cell, 2000 µg/mL of unloaded NPs, and Scr-siRNA NPs were used as controls. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Figure Legend Snippet: Cellular internalization of siRNA NPs was investigated. ( a ) Histogram analyzed by flow cytometry demonstrated cellular internalized NPs at 1 h. ( b ) Mean Fluorescence Intensity of internalized NPs at different concentrations for 1 h. ( c ) Different concentrations of BLOCK-iT NPs were treated with HepG2 cells at different incubation times. ( d ) The percentage of HepG2 cell viability at different concentrations of AFP -siRNA NPs for 48 h. Untreated HepG2 cell, 2000 µg/mL of unloaded NPs, and Scr-siRNA NPs were used as controls. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).

    Techniques Used: Flow Cytometry, Fluorescence, Blocking Assay, Incubation

    Antiproliferation and gene silencing effects of sorafenib or sunitinib in combination with AFP -siRNA loaded NPs on HepG2 cell line. ( a ) Percentage of cell viability of the cells. ( b ) Caspase 3/7 activity of the cells when treated with either single or combined treatments of AFP -siRNA NP and drugs. ( c ) Gene silencing effect of combined treatments of siRNA and drugs compared to the single use of AFP -siRNA NPs alone. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).
    Figure Legend Snippet: Antiproliferation and gene silencing effects of sorafenib or sunitinib in combination with AFP -siRNA loaded NPs on HepG2 cell line. ( a ) Percentage of cell viability of the cells. ( b ) Caspase 3/7 activity of the cells when treated with either single or combined treatments of AFP -siRNA NP and drugs. ( c ) Gene silencing effect of combined treatments of siRNA and drugs compared to the single use of AFP -siRNA NPs alone. Significance was set at p -values of p < 0.05 and indicated with an asterisk (*).

    Techniques Used: Activity Assay


    Structured Review

    Shanghai Genechem Ltd cell lines bel7402 sirna afp
    Localization of AFP 390–609 fragment (or named AFP fragment) in <t>Bel7402</t> and L‐02 cells. (A) Bel7402 and L‐02 cells were added with AFP fragment, followed by culturing at 37°C in a humidified atmosphere of 5% CO 2 . The images were captured under laser confocal microscopy. Localization of AFP fragment was visualized in Bel7402 (HCC cells with high AFP receptor expressed) but not in L‐02 cells (normal hepatic cell). (B) Localization of AFP fragment in Bel7402 was magnification. Nuclei are stained with DAPI (blue). AFP fragment was labelled with FITC (green). Three independent experiments were performed for these data.
    Cell Lines Bel7402 Sirna Afp, supplied by Shanghai Genechem Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "α‐Fetoprotein fragment synergizes with sorafenib to inhibit hepatoma cell growth and migration and promote the apoptosis"

    Article Title: α‐Fetoprotein fragment synergizes with sorafenib to inhibit hepatoma cell growth and migration and promote the apoptosis

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.17565

    Localization of AFP 390–609 fragment (or named AFP fragment) in Bel7402 and L‐02 cells. (A) Bel7402 and L‐02 cells were added with AFP fragment, followed by culturing at 37°C in a humidified atmosphere of 5% CO 2 . The images were captured under laser confocal microscopy. Localization of AFP fragment was visualized in Bel7402 (HCC cells with high AFP receptor expressed) but not in L‐02 cells (normal hepatic cell). (B) Localization of AFP fragment in Bel7402 was magnification. Nuclei are stained with DAPI (blue). AFP fragment was labelled with FITC (green). Three independent experiments were performed for these data.
    Figure Legend Snippet: Localization of AFP 390–609 fragment (or named AFP fragment) in Bel7402 and L‐02 cells. (A) Bel7402 and L‐02 cells were added with AFP fragment, followed by culturing at 37°C in a humidified atmosphere of 5% CO 2 . The images were captured under laser confocal microscopy. Localization of AFP fragment was visualized in Bel7402 (HCC cells with high AFP receptor expressed) but not in L‐02 cells (normal hepatic cell). (B) Localization of AFP fragment in Bel7402 was magnification. Nuclei are stained with DAPI (blue). AFP fragment was labelled with FITC (green). Three independent experiments were performed for these data.

    Techniques Used: Confocal Microscopy, Staining

    Effects of and AFP 390–609 (AFP fragment) and sorafenib on the growth of human hepatoma cells Bel7402 and human normal liver cells L‐02. (A) The human hepatoma cell line Bel7402 and normal hepatic cell L‐02 were treated with AFP fragment at concentrations of 0–350 μg/ml. The viability of the cells was analysed by MTT. (B) Bel7402 and (C) L‐02 cells were treated with sorafenib at concentrations of 2.5–20 μg/ml; at the same time synergized with AFP fragment at concentrations of 100, 200 and 300 μg/ml for 72 h. The viability of the cells was analysed by MTT. ** p < 0.01 and *** p < 0.001 versus untreated groups. N = 3.
    Figure Legend Snippet: Effects of and AFP 390–609 (AFP fragment) and sorafenib on the growth of human hepatoma cells Bel7402 and human normal liver cells L‐02. (A) The human hepatoma cell line Bel7402 and normal hepatic cell L‐02 were treated with AFP fragment at concentrations of 0–350 μg/ml. The viability of the cells was analysed by MTT. (B) Bel7402 and (C) L‐02 cells were treated with sorafenib at concentrations of 2.5–20 μg/ml; at the same time synergized with AFP fragment at concentrations of 100, 200 and 300 μg/ml for 72 h. The viability of the cells was analysed by MTT. ** p < 0.01 and *** p < 0.001 versus untreated groups. N = 3.

    Techniques Used:

    AFP 390–609 fragment synergizes with sorafenib to inhibit scratch repair and migration of Bel7402 cells. (A) Bel7402 cells were treated with sorafenib (5 μg/ml); sorafenib (5 μg/ml) + AP fragment (200 μg/ml); or Bel7402‐siRNA‐AFP cells were treated with sorafenib (5 μg/ml) for 72 h; and the scratch area of Bel7402 cells covered was detected in scratch repair experiment. (B) The column picture indicates the statistical analysis of the cell repair ratio. (C) The migration of Bel7402 cells and Bel7402‐siRNA‐AFP cells (treated as A) was detected in a transwell chamber. (D) The column picture indicates the statistical analysis of the migratory cell ratio. *** p < 0.001 versus control groups. The images are representative of at least three independent experiments.
    Figure Legend Snippet: AFP 390–609 fragment synergizes with sorafenib to inhibit scratch repair and migration of Bel7402 cells. (A) Bel7402 cells were treated with sorafenib (5 μg/ml); sorafenib (5 μg/ml) + AP fragment (200 μg/ml); or Bel7402‐siRNA‐AFP cells were treated with sorafenib (5 μg/ml) for 72 h; and the scratch area of Bel7402 cells covered was detected in scratch repair experiment. (B) The column picture indicates the statistical analysis of the cell repair ratio. (C) The migration of Bel7402 cells and Bel7402‐siRNA‐AFP cells (treated as A) was detected in a transwell chamber. (D) The column picture indicates the statistical analysis of the migratory cell ratio. *** p < 0.001 versus control groups. The images are representative of at least three independent experiments.

    Techniques Used: Migration

    AFP 390–609 fragment synergizes with sorafenib to promote the apoptosis of Bel7402 cells. (A) Bel7402 cells were treated with sorafenib (10 μg/ml), sorafenib (10 μg/ml) + AFP fragment (200 μg/ml) or Bel7402‐siRNA‐AFP cells treated with sorafenib (10 μg/ml) for 24 h; the apoptosis of Bel7402 cells was analysed by flow cytometry. (B) The column picture shows the statistical analysis of the apoptosis ratios; *** p < 0.001 versus control groups. The images are representative of at least three independent experiments.
    Figure Legend Snippet: AFP 390–609 fragment synergizes with sorafenib to promote the apoptosis of Bel7402 cells. (A) Bel7402 cells were treated with sorafenib (10 μg/ml), sorafenib (10 μg/ml) + AFP fragment (200 μg/ml) or Bel7402‐siRNA‐AFP cells treated with sorafenib (10 μg/ml) for 24 h; the apoptosis of Bel7402 cells was analysed by flow cytometry. (B) The column picture shows the statistical analysis of the apoptosis ratios; *** p < 0.001 versus control groups. The images are representative of at least three independent experiments.

    Techniques Used: Flow Cytometry

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    Axin 1 overexpression reduced <t>AFP-mediated</t> Wnt-pathway activation and malignancy in established APGC cells. Notes: ( A – D ) After Axin 1 knockdown using <t>siRNAs</t> in GC cells and ( E – H ) Axin 1 overexpression in AFP-overexpressing GC cells for 48 hours, Wnt-signaling-involved protein-expression levels, β-catenin-mediated TCF transcriptional activity, and cell-proliferation, -invasion, and -migration abilities were determined by immunoblotting, dual-luciferase, CCK8, and transwell assays, respectively. Data expressed as mean ± SD. * P <0.05 by ANOVA. Abbreviation: APGC, AFP-producing gastric cancer.
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    A, Human liver cancer <t>PLC/PRF/5(AFP-producing)</t> cell line was transfected with <t>AFP-siRNA</t> vectors for 48 hours and CXCR4 expression was observed by laser confocal microscopy. B, human liver HLE(non-AFP-producing) cell lines were transfected with pcDNA3.1- afp vector for 48 hours and CXCR4 expression was observed by laser confocal microscopy. The images were taken of three independent experiments.
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    A, Human liver cancer <t>PLC/PRF/5(AFP-producing)</t> cell line was transfected with <t>AFP-siRNA</t> vectors for 48 hours and CXCR4 expression was observed by laser confocal microscopy. B, human liver HLE(non-AFP-producing) cell lines were transfected with pcDNA3.1- afp vector for 48 hours and CXCR4 expression was observed by laser confocal microscopy. The images were taken of three independent experiments.
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    Shanghai Genechem Ltd cell lines bel7402 sirna afp
    Localization of AFP 390–609 fragment (or named AFP fragment) in <t>Bel7402</t> and L‐02 cells. (A) Bel7402 and L‐02 cells were added with AFP fragment, followed by culturing at 37°C in a humidified atmosphere of 5% CO 2 . The images were captured under laser confocal microscopy. Localization of AFP fragment was visualized in Bel7402 (HCC cells with high AFP receptor expressed) but not in L‐02 cells (normal hepatic cell). (B) Localization of AFP fragment in Bel7402 was magnification. Nuclei are stained with DAPI (blue). AFP fragment was labelled with FITC (green). Three independent experiments were performed for these data.
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    Axin 1 overexpression reduced AFP-mediated Wnt-pathway activation and malignancy in established APGC cells. Notes: ( A – D ) After Axin 1 knockdown using siRNAs in GC cells and ( E – H ) Axin 1 overexpression in AFP-overexpressing GC cells for 48 hours, Wnt-signaling-involved protein-expression levels, β-catenin-mediated TCF transcriptional activity, and cell-proliferation, -invasion, and -migration abilities were determined by immunoblotting, dual-luciferase, CCK8, and transwell assays, respectively. Data expressed as mean ± SD. * P <0.05 by ANOVA. Abbreviation: APGC, AFP-producing gastric cancer.

    Journal: Cancer Management and Research

    Article Title: Activated Wnt signaling promotes growth and progression of AFP-producing gastric cancer in preclinical models

    doi: 10.2147/CMAR.S187219

    Figure Lengend Snippet: Axin 1 overexpression reduced AFP-mediated Wnt-pathway activation and malignancy in established APGC cells. Notes: ( A – D ) After Axin 1 knockdown using siRNAs in GC cells and ( E – H ) Axin 1 overexpression in AFP-overexpressing GC cells for 48 hours, Wnt-signaling-involved protein-expression levels, β-catenin-mediated TCF transcriptional activity, and cell-proliferation, -invasion, and -migration abilities were determined by immunoblotting, dual-luciferase, CCK8, and transwell assays, respectively. Data expressed as mean ± SD. * P <0.05 by ANOVA. Abbreviation: APGC, AFP-producing gastric cancer.

    Article Snippet: HepG2 cells were transfected with AFP siRNAs and their negative control siRNA using an AFP-siRNA kit (RiboBio, Guangzhou, China), GC cells with Axin 1 siRNAs, and their negative control siRNA using an Axin 1-siRNA kit (RiboBio) and AFP-overexpressing GC cells with Axin 1 and its control plasmids (Vigene Biosciences, Jinan, China).

    Techniques: Over Expression, Activation Assay, Expressing, Activity Assay, Migration, Western Blot, Luciferase

    A, Human liver cancer PLC/PRF/5(AFP-producing) cell line was transfected with AFP-siRNA vectors for 48 hours and CXCR4 expression was observed by laser confocal microscopy. B, human liver HLE(non-AFP-producing) cell lines were transfected with pcDNA3.1- afp vector for 48 hours and CXCR4 expression was observed by laser confocal microscopy. The images were taken of three independent experiments.

    Journal: Oncoscience

    Article Title: Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells

    doi:

    Figure Lengend Snippet: A, Human liver cancer PLC/PRF/5(AFP-producing) cell line was transfected with AFP-siRNA vectors for 48 hours and CXCR4 expression was observed by laser confocal microscopy. B, human liver HLE(non-AFP-producing) cell lines were transfected with pcDNA3.1- afp vector for 48 hours and CXCR4 expression was observed by laser confocal microscopy. The images were taken of three independent experiments.

    Article Snippet: After the transfection was induced by Lipofectamine 2000(Invitrogen, Carlsbad, CA, USA), the AFP-producing PLC/PRF/5 cells were incubated with AFP-siRNA for 6 h and the medium was replaced with complete medium for 42 h. The cells were then harvested for western blotting, co-immunoprecipitation(Co-IP) and chromatin immunoprecipitation(ChIP) assay.

    Techniques: Transfection, Expressing, Confocal Microscopy, Plasmid Preparation

    A, Expressions of AFP, CXCR4, and PTEN in PLC/PRF/5 or HLE cells were analyzed by Western blotting. B, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours and the expressions of AFP, p-AKT(Ser473), and CXCR4 were analyzed by Western blotting, low column graph indicated the quantity of protein expression. C, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours and the expressions of AFP, p-AKT(Ser473), and CXCR4 in HLE cells were analyzed by Western blotting, low column graph indicated the quantity of protein expression. We performed three separate experiments in triplicate.

    Journal: Oncoscience

    Article Title: Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells

    doi:

    Figure Lengend Snippet: A, Expressions of AFP, CXCR4, and PTEN in PLC/PRF/5 or HLE cells were analyzed by Western blotting. B, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours and the expressions of AFP, p-AKT(Ser473), and CXCR4 were analyzed by Western blotting, low column graph indicated the quantity of protein expression. C, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours and the expressions of AFP, p-AKT(Ser473), and CXCR4 in HLE cells were analyzed by Western blotting, low column graph indicated the quantity of protein expression. We performed three separate experiments in triplicate.

    Article Snippet: After the transfection was induced by Lipofectamine 2000(Invitrogen, Carlsbad, CA, USA), the AFP-producing PLC/PRF/5 cells were incubated with AFP-siRNA for 6 h and the medium was replaced with complete medium for 42 h. The cells were then harvested for western blotting, co-immunoprecipitation(Co-IP) and chromatin immunoprecipitation(ChIP) assay.

    Techniques: Western Blot, Transfection, Expressing

    A, Co-location of AFP and PTEN in PLC/PRF/5 or HLE cells were observed by laser confocal microscopy. B, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours and the interaction of AFP with PTEN was detected by co-immunoprecipitation(Co-IP). C, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours and the interaction of AFP with PTEN was detected by Co-IP. We performed separate experiments in triplicate.

    Journal: Oncoscience

    Article Title: Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells

    doi:

    Figure Lengend Snippet: A, Co-location of AFP and PTEN in PLC/PRF/5 or HLE cells were observed by laser confocal microscopy. B, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours and the interaction of AFP with PTEN was detected by co-immunoprecipitation(Co-IP). C, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours and the interaction of AFP with PTEN was detected by Co-IP. We performed separate experiments in triplicate.

    Article Snippet: After the transfection was induced by Lipofectamine 2000(Invitrogen, Carlsbad, CA, USA), the AFP-producing PLC/PRF/5 cells were incubated with AFP-siRNA for 6 h and the medium was replaced with complete medium for 42 h. The cells were then harvested for western blotting, co-immunoprecipitation(Co-IP) and chromatin immunoprecipitation(ChIP) assay.

    Techniques: Confocal Microscopy, Transfection, Immunoprecipitation, Co-Immunoprecipitation Assay

    A, PLC/PRF/5 cells were treated with a potent inhibitor of PI3K/AKT, Ly294002 (20μmol/L), or transfected with AFP-siRNA for 48 hours, and the expressions of p-mTOR(Ser2448) and CXCR4 were analyzed by Western blotting, right column graph indicated the quantity of protein expression, * P <0.01 vs non-treated(control) group and scramble-siRNA vectors treated group; # P <0.01 vs control group and scramble-siRNA vectors treated group. B, HLE cells were treated with Ly294002 (20μmol/L) or pcDNA3.1- afp vectors or co-treated with Ly294002 (20μg/mL) and pcDNA3.1- afp for 48 hours, and the expressions of p-mTOR(Ser2448) and CXCR4 were analyzed by Western blotting, right column graph indicated the quantity of protein expression, * P <0.01 vs control group and pcDNA3.1 empty vectors treated group; # P <0.01 vs control group, Ly294002 treated group, pcDNA3.1 empty vectors treated group, and co-treated with Ly294002 (20 μg/mL) and pcDNA3.1- afp group. We performed separate experiments in triplicate.

    Journal: Oncoscience

    Article Title: Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells

    doi:

    Figure Lengend Snippet: A, PLC/PRF/5 cells were treated with a potent inhibitor of PI3K/AKT, Ly294002 (20μmol/L), or transfected with AFP-siRNA for 48 hours, and the expressions of p-mTOR(Ser2448) and CXCR4 were analyzed by Western blotting, right column graph indicated the quantity of protein expression, * P <0.01 vs non-treated(control) group and scramble-siRNA vectors treated group; # P <0.01 vs control group and scramble-siRNA vectors treated group. B, HLE cells were treated with Ly294002 (20μmol/L) or pcDNA3.1- afp vectors or co-treated with Ly294002 (20μg/mL) and pcDNA3.1- afp for 48 hours, and the expressions of p-mTOR(Ser2448) and CXCR4 were analyzed by Western blotting, right column graph indicated the quantity of protein expression, * P <0.01 vs control group and pcDNA3.1 empty vectors treated group; # P <0.01 vs control group, Ly294002 treated group, pcDNA3.1 empty vectors treated group, and co-treated with Ly294002 (20 μg/mL) and pcDNA3.1- afp group. We performed separate experiments in triplicate.

    Article Snippet: After the transfection was induced by Lipofectamine 2000(Invitrogen, Carlsbad, CA, USA), the AFP-producing PLC/PRF/5 cells were incubated with AFP-siRNA for 6 h and the medium was replaced with complete medium for 42 h. The cells were then harvested for western blotting, co-immunoprecipitation(Co-IP) and chromatin immunoprecipitation(ChIP) assay.

    Techniques: Transfection, Western Blot, Expressing

    A, PLC/PRF/5 cells were transfected with AFP-siRNA for 48 hours, and the combination of p-mTOR(Ser2448) and the CXCR4 gene promoter was evidenced by chromatin immunoprecipitation(ChIP). B, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours, and the combination of p-mTOR(Ser2448) with the CXCR4 gene promoter was evidenced by ChIP. We performed separate experiments in triplicate.

    Journal: Oncoscience

    Article Title: Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells

    doi:

    Figure Lengend Snippet: A, PLC/PRF/5 cells were transfected with AFP-siRNA for 48 hours, and the combination of p-mTOR(Ser2448) and the CXCR4 gene promoter was evidenced by chromatin immunoprecipitation(ChIP). B, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours, and the combination of p-mTOR(Ser2448) with the CXCR4 gene promoter was evidenced by ChIP. We performed separate experiments in triplicate.

    Article Snippet: After the transfection was induced by Lipofectamine 2000(Invitrogen, Carlsbad, CA, USA), the AFP-producing PLC/PRF/5 cells were incubated with AFP-siRNA for 6 h and the medium was replaced with complete medium for 42 h. The cells were then harvested for western blotting, co-immunoprecipitation(Co-IP) and chromatin immunoprecipitation(ChIP) assay.

    Techniques: Transfection, Chromatin Immunoprecipitation

    A, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours, wound healing were observed by microscopy, and migratory cells were analyzed by a transwell chamber assay. B, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours, wound healing of the cells were observed by microscopy, and migratory cells were analyzed by a transwell chamber assay. ** P <0.01. We performed three independent experiments.

    Journal: Oncoscience

    Article Title: Alpha-fetoprotein activates AKT/mTOR signaling to promote CXCR4 expression and migration of hepatoma cells

    doi:

    Figure Lengend Snippet: A, PLC/PRF/5 cells were transfected with AFP-siRNA vectors for 48 hours, wound healing were observed by microscopy, and migratory cells were analyzed by a transwell chamber assay. B, HLE cells were transfected with pcDNA3.1- afp vectors for 48 hours, wound healing of the cells were observed by microscopy, and migratory cells were analyzed by a transwell chamber assay. ** P <0.01. We performed three independent experiments.

    Article Snippet: After the transfection was induced by Lipofectamine 2000(Invitrogen, Carlsbad, CA, USA), the AFP-producing PLC/PRF/5 cells were incubated with AFP-siRNA for 6 h and the medium was replaced with complete medium for 42 h. The cells were then harvested for western blotting, co-immunoprecipitation(Co-IP) and chromatin immunoprecipitation(ChIP) assay.

    Techniques: Transfection, Microscopy, Transwell Chamber Assay

    Localization of AFP 390–609 fragment (or named AFP fragment) in Bel7402 and L‐02 cells. (A) Bel7402 and L‐02 cells were added with AFP fragment, followed by culturing at 37°C in a humidified atmosphere of 5% CO 2 . The images were captured under laser confocal microscopy. Localization of AFP fragment was visualized in Bel7402 (HCC cells with high AFP receptor expressed) but not in L‐02 cells (normal hepatic cell). (B) Localization of AFP fragment in Bel7402 was magnification. Nuclei are stained with DAPI (blue). AFP fragment was labelled with FITC (green). Three independent experiments were performed for these data.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: α‐Fetoprotein fragment synergizes with sorafenib to inhibit hepatoma cell growth and migration and promote the apoptosis

    doi: 10.1111/jcmm.17565

    Figure Lengend Snippet: Localization of AFP 390–609 fragment (or named AFP fragment) in Bel7402 and L‐02 cells. (A) Bel7402 and L‐02 cells were added with AFP fragment, followed by culturing at 37°C in a humidified atmosphere of 5% CO 2 . The images were captured under laser confocal microscopy. Localization of AFP fragment was visualized in Bel7402 (HCC cells with high AFP receptor expressed) but not in L‐02 cells (normal hepatic cell). (B) Localization of AFP fragment in Bel7402 was magnification. Nuclei are stained with DAPI (blue). AFP fragment was labelled with FITC (green). Three independent experiments were performed for these data.

    Article Snippet: Then, they were transfected of siRNA‐AFP Lentivirus (HitransG A; Shanghai Genechem Co., Ltd.) The cell lines Bel7402‐siRNA‐AFP which were interfered AFP expression by siRNA were screened by puromycin (Beyotime Biotechnology) after 72 h. The siRNA sequence is as follows: 5′‐ccCTCTTGAATGCCAAGATAA‐3′, use vector GV493 ( http://www.genechem.com.cn/service/index.php?ac=gene&at=vector_search&keyword=GV493 ).

    Techniques: Confocal Microscopy, Staining

    Effects of and AFP 390–609 (AFP fragment) and sorafenib on the growth of human hepatoma cells Bel7402 and human normal liver cells L‐02. (A) The human hepatoma cell line Bel7402 and normal hepatic cell L‐02 were treated with AFP fragment at concentrations of 0–350 μg/ml. The viability of the cells was analysed by MTT. (B) Bel7402 and (C) L‐02 cells were treated with sorafenib at concentrations of 2.5–20 μg/ml; at the same time synergized with AFP fragment at concentrations of 100, 200 and 300 μg/ml for 72 h. The viability of the cells was analysed by MTT. ** p < 0.01 and *** p < 0.001 versus untreated groups. N = 3.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: α‐Fetoprotein fragment synergizes with sorafenib to inhibit hepatoma cell growth and migration and promote the apoptosis

    doi: 10.1111/jcmm.17565

    Figure Lengend Snippet: Effects of and AFP 390–609 (AFP fragment) and sorafenib on the growth of human hepatoma cells Bel7402 and human normal liver cells L‐02. (A) The human hepatoma cell line Bel7402 and normal hepatic cell L‐02 were treated with AFP fragment at concentrations of 0–350 μg/ml. The viability of the cells was analysed by MTT. (B) Bel7402 and (C) L‐02 cells were treated with sorafenib at concentrations of 2.5–20 μg/ml; at the same time synergized with AFP fragment at concentrations of 100, 200 and 300 μg/ml for 72 h. The viability of the cells was analysed by MTT. ** p < 0.01 and *** p < 0.001 versus untreated groups. N = 3.

    Article Snippet: Then, they were transfected of siRNA‐AFP Lentivirus (HitransG A; Shanghai Genechem Co., Ltd.) The cell lines Bel7402‐siRNA‐AFP which were interfered AFP expression by siRNA were screened by puromycin (Beyotime Biotechnology) after 72 h. The siRNA sequence is as follows: 5′‐ccCTCTTGAATGCCAAGATAA‐3′, use vector GV493 ( http://www.genechem.com.cn/service/index.php?ac=gene&at=vector_search&keyword=GV493 ).

    Techniques:

    AFP 390–609 fragment synergizes with sorafenib to inhibit scratch repair and migration of Bel7402 cells. (A) Bel7402 cells were treated with sorafenib (5 μg/ml); sorafenib (5 μg/ml) + AP fragment (200 μg/ml); or Bel7402‐siRNA‐AFP cells were treated with sorafenib (5 μg/ml) for 72 h; and the scratch area of Bel7402 cells covered was detected in scratch repair experiment. (B) The column picture indicates the statistical analysis of the cell repair ratio. (C) The migration of Bel7402 cells and Bel7402‐siRNA‐AFP cells (treated as A) was detected in a transwell chamber. (D) The column picture indicates the statistical analysis of the migratory cell ratio. *** p < 0.001 versus control groups. The images are representative of at least three independent experiments.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: α‐Fetoprotein fragment synergizes with sorafenib to inhibit hepatoma cell growth and migration and promote the apoptosis

    doi: 10.1111/jcmm.17565

    Figure Lengend Snippet: AFP 390–609 fragment synergizes with sorafenib to inhibit scratch repair and migration of Bel7402 cells. (A) Bel7402 cells were treated with sorafenib (5 μg/ml); sorafenib (5 μg/ml) + AP fragment (200 μg/ml); or Bel7402‐siRNA‐AFP cells were treated with sorafenib (5 μg/ml) for 72 h; and the scratch area of Bel7402 cells covered was detected in scratch repair experiment. (B) The column picture indicates the statistical analysis of the cell repair ratio. (C) The migration of Bel7402 cells and Bel7402‐siRNA‐AFP cells (treated as A) was detected in a transwell chamber. (D) The column picture indicates the statistical analysis of the migratory cell ratio. *** p < 0.001 versus control groups. The images are representative of at least three independent experiments.

    Article Snippet: Then, they were transfected of siRNA‐AFP Lentivirus (HitransG A; Shanghai Genechem Co., Ltd.) The cell lines Bel7402‐siRNA‐AFP which were interfered AFP expression by siRNA were screened by puromycin (Beyotime Biotechnology) after 72 h. The siRNA sequence is as follows: 5′‐ccCTCTTGAATGCCAAGATAA‐3′, use vector GV493 ( http://www.genechem.com.cn/service/index.php?ac=gene&at=vector_search&keyword=GV493 ).

    Techniques: Migration

    AFP 390–609 fragment synergizes with sorafenib to promote the apoptosis of Bel7402 cells. (A) Bel7402 cells were treated with sorafenib (10 μg/ml), sorafenib (10 μg/ml) + AFP fragment (200 μg/ml) or Bel7402‐siRNA‐AFP cells treated with sorafenib (10 μg/ml) for 24 h; the apoptosis of Bel7402 cells was analysed by flow cytometry. (B) The column picture shows the statistical analysis of the apoptosis ratios; *** p < 0.001 versus control groups. The images are representative of at least three independent experiments.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: α‐Fetoprotein fragment synergizes with sorafenib to inhibit hepatoma cell growth and migration and promote the apoptosis

    doi: 10.1111/jcmm.17565

    Figure Lengend Snippet: AFP 390–609 fragment synergizes with sorafenib to promote the apoptosis of Bel7402 cells. (A) Bel7402 cells were treated with sorafenib (10 μg/ml), sorafenib (10 μg/ml) + AFP fragment (200 μg/ml) or Bel7402‐siRNA‐AFP cells treated with sorafenib (10 μg/ml) for 24 h; the apoptosis of Bel7402 cells was analysed by flow cytometry. (B) The column picture shows the statistical analysis of the apoptosis ratios; *** p < 0.001 versus control groups. The images are representative of at least three independent experiments.

    Article Snippet: Then, they were transfected of siRNA‐AFP Lentivirus (HitransG A; Shanghai Genechem Co., Ltd.) The cell lines Bel7402‐siRNA‐AFP which were interfered AFP expression by siRNA were screened by puromycin (Beyotime Biotechnology) after 72 h. The siRNA sequence is as follows: 5′‐ccCTCTTGAATGCCAAGATAA‐3′, use vector GV493 ( http://www.genechem.com.cn/service/index.php?ac=gene&at=vector_search&keyword=GV493 ).

    Techniques: Flow Cytometry