aflii  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94

    Structured Review

    New England Biolabs aflii
    Aflii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aflii/product/New England Biolabs
    Average 94 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    aflii - by Bioz Stars, 2020-04
    94/100 stars

    Images

    Related Articles

    In Vitro:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline.

    Mutagenesis:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline.

    Incubation:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline.

    Mouse Assay:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline.

    Generated:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline.

    Activity Assay:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline.

    Polymerase Chain Reaction:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. ..

    Injection:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline.

    Purification:

    Article Title: The Initiation of Meiotic Sex Chromosome Inactivation Sequesters DNA Damage Signaling from Autosomes in Mouse Spermatogenesis.
    Article Snippet: Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline. .. Meiotic sex chromosome inactivation (MSCI) is an essential event in the mammalian male germline.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    New England Biolabs aflii
    Generation of vPK transgenic mice. FLAG-tagged vPK was cloned, using HinDIII-HF and <t>BamHI-HF,</t> into a plasmid containing a Ub- and hGH-stabilization element. The linearized transgene fragment was microinjected into the embryos of C57BL/6 mice and implanted into pseudopregnant female mice. Each vPK line was developed from breeding a vPK founder to a C57BL/6 mouse. ( A ) Schematic of the linearized construct used to generate the vPK transgenic mice, as well as the cut sites and probe for the Southern blot in D . ( B ) DNA from the tails of 2 WT (wA and wB) and 2 vPK transgenic mice (vA and vB) for lines 1 and 2 was isolated and evaluated by PCR for the vPK transgene. ( C ) Expression of vPK protein in lysates from spleens of the mice in B as determined by SDS-PAGE and Western blot. Lysate from HEK-293 cells that transiently express vPK was used as a positive control for vPK expression. ( D ) Southern blot of WT, vPK1, and vPK2. DNA was isolated from the spleens of WT and vPK lines 1 and 2. D, double digest with HinDIII-HF and BamHI-HF; S, single digestion with <t>AFlII;</t> p-vPK, plasmid Ub.vPK.hGH.
    Aflii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aflii/product/New England Biolabs
    Average 99 stars, based on 55 article reviews
    Price from $9.99 to $1999.99
    aflii - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    86
    New England Biolabs aflii hf
    Construction and quantitation of rKSHV.294. (A) Schematic diagram of rKSHV.294 showing the insertion site in the KSHV genome; the BamHI sites flanking the 4.8-kb segment of the KSHV genome used are indicated. The relative positions of the SeAP, the GFP, and the Neo elements are shown with their respective promoters but not to scale. Beneath the 294 construct are shown the expected PCR products (a to e) with the primers used for analysis of viral <t>DNA.</t> (B) Hybridization analysis of rKSHV.294 and JSC-1 viral DNA. (Left) KSHV probe using the 4.8-kb BamHI fragment used to construct the virus. (Right) Neo probe. Lane 1, rKSHV.294 × <t>AflII,</t> predicted fragments of 15.9, 5.7, and 4.8 kb. The 4.8-kb fragment contains the SeAP/GFP/Neo insert with only 170 bp of KSHV DNA, which accounts for the weak band. Lane 2, JSC-1 × AflII, predicted fragments of 15.9 and 5.8 kb. Lane 3, rKSHV.294 × SspI, predicted fragments of 8 and 6.2 kb. Lane 4, JSC-1 × SspI, predicted fragments of 6.2 and 3.3 kb. Lane 5, rKSHV.294 × AflII, predicted fragment of 4.8 kb. Lane 6, JSC-1 × AflII. Lane 7, rKSHV.294 DNA × SspI, predicted fragment of 8 kb. Lane 8, JSC-1 × SspI. DNA markers in kb are on the left side. (C) Ethidium bromide-stained agarose gel following electrophoresis of the PCR products resulting with rKSHV.294 viral DNA with the indicated primers described in Materials and Methods. Lane a, ORF56f (outside BamHI site) and SeAPr; lane b, ORF57u and SeAPr; lane c, SeAPf and GFPf; lane d, GFPr and Neor; lane e, Neof and K9. (D) rKSHV.294 was tested for tTA control of SeAP expression by the infection of 293 cells or of 293 cells expressing tTA at an MOI of
    Aflii Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aflii hf/product/New England Biolabs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    aflii hf - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    Image Search Results


    Generation of vPK transgenic mice. FLAG-tagged vPK was cloned, using HinDIII-HF and BamHI-HF, into a plasmid containing a Ub- and hGH-stabilization element. The linearized transgene fragment was microinjected into the embryos of C57BL/6 mice and implanted into pseudopregnant female mice. Each vPK line was developed from breeding a vPK founder to a C57BL/6 mouse. ( A ) Schematic of the linearized construct used to generate the vPK transgenic mice, as well as the cut sites and probe for the Southern blot in D . ( B ) DNA from the tails of 2 WT (wA and wB) and 2 vPK transgenic mice (vA and vB) for lines 1 and 2 was isolated and evaluated by PCR for the vPK transgene. ( C ) Expression of vPK protein in lysates from spleens of the mice in B as determined by SDS-PAGE and Western blot. Lysate from HEK-293 cells that transiently express vPK was used as a positive control for vPK expression. ( D ) Southern blot of WT, vPK1, and vPK2. DNA was isolated from the spleens of WT and vPK lines 1 and 2. D, double digest with HinDIII-HF and BamHI-HF; S, single digestion with AFlII; p-vPK, plasmid Ub.vPK.hGH.

    Journal: The Journal of Clinical Investigation

    Article Title: Human herpesvirus–encoded kinase induces B cell lymphomas in vivo

    doi: 10.1172/JCI97053

    Figure Lengend Snippet: Generation of vPK transgenic mice. FLAG-tagged vPK was cloned, using HinDIII-HF and BamHI-HF, into a plasmid containing a Ub- and hGH-stabilization element. The linearized transgene fragment was microinjected into the embryos of C57BL/6 mice and implanted into pseudopregnant female mice. Each vPK line was developed from breeding a vPK founder to a C57BL/6 mouse. ( A ) Schematic of the linearized construct used to generate the vPK transgenic mice, as well as the cut sites and probe for the Southern blot in D . ( B ) DNA from the tails of 2 WT (wA and wB) and 2 vPK transgenic mice (vA and vB) for lines 1 and 2 was isolated and evaluated by PCR for the vPK transgene. ( C ) Expression of vPK protein in lysates from spleens of the mice in B as determined by SDS-PAGE and Western blot. Lysate from HEK-293 cells that transiently express vPK was used as a positive control for vPK expression. ( D ) Southern blot of WT, vPK1, and vPK2. DNA was isolated from the spleens of WT and vPK lines 1 and 2. D, double digest with HinDIII-HF and BamHI-HF; S, single digestion with AFlII; p-vPK, plasmid Ub.vPK.hGH.

    Article Snippet: Fifteen micrograms of DNA from each mouse was double digested with BamHI-HF and HindIII-HF (New England BioLabs) and single digested using AflII (New England BioLabs) in a total volume of 100 μl at 37°C overnight.

    Techniques: Transgenic Assay, Mouse Assay, Clone Assay, Plasmid Preparation, Construct, Southern Blot, Western Blot, Isolation, Polymerase Chain Reaction, Expressing, SDS Page, Positive Control

    In silico evaluation of 236 unique restriction enzyme sequence/cut sites according to frequency metrics cut1 , min100 and max10k . Regimes of low-frequency and high-frequency cutters are highlighted. Best enzymes are found where metrics values are highest. ( a ) max10k values for building blocks cluster ∼1.0 as essentially all have total size (including vector) below 10 kb. In contrast, max10k is discriminating for assemblies (values range from 0.75 to 10) due to larger size distribution. ( b ) Screening against 2236 cloned building blocks using metrics cut1 and min100 . Most suitable enzymes are Afl III and Ava II. ( c ) Screening against 5660 assemblies using metrics cut1 , min100 and max10k . Most suitable enzyme is Bsr DI.

    Journal: Nucleic Acids Research

    Article Title: High-throughput, cost-effective verification of structural DNA assembly

    doi: 10.1093/nar/gkt1088

    Figure Lengend Snippet: In silico evaluation of 236 unique restriction enzyme sequence/cut sites according to frequency metrics cut1 , min100 and max10k . Regimes of low-frequency and high-frequency cutters are highlighted. Best enzymes are found where metrics values are highest. ( a ) max10k values for building blocks cluster ∼1.0 as essentially all have total size (including vector) below 10 kb. In contrast, max10k is discriminating for assemblies (values range from 0.75 to 10) due to larger size distribution. ( b ) Screening against 2236 cloned building blocks using metrics cut1 and min100 . Most suitable enzymes are Afl III and Ava II. ( c ) Screening against 5660 assemblies using metrics cut1 , min100 and max10k . Most suitable enzyme is Bsr DI.

    Article Snippet: Digest formulations and incubation temperatures for Bsr DI, Afl II and Ava II (NEB) are given in Supplementary Table S2 .

    Techniques: In Silico, Sequencing, Plasmid Preparation, Clone Assay, Antiviral Assay

    Screening and confirmation of recombinant clones without the kanamycin unit. A: Diagram of the removal of the Kam unit in SW105 E. coli ; B: Electrophoretogram of the PCR product according to the recombinant BAC template with the 5arm primer; C: Electrophoretogram of the PCR product according to the recombinant BAC template with different primers; D: Electrophoretogram of the PCR product with BamH I or AflI I digestion. M: Marker; OB: Original BAC; RB: Recombinant BAC; DkB: Deleted kanamycin BAC; 5F: Forward primer of the 5arm; 5R: Reverse primer of the 5arm; 3F: Forward primer of the 3arm; 3R: Reverse primer of the 3arm; Cre: Cre primer.

    Journal: World Journal of Gastroenterology

    Article Title: Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

    doi: 10.3748/wjg.v23.i2.224

    Figure Lengend Snippet: Screening and confirmation of recombinant clones without the kanamycin unit. A: Diagram of the removal of the Kam unit in SW105 E. coli ; B: Electrophoretogram of the PCR product according to the recombinant BAC template with the 5arm primer; C: Electrophoretogram of the PCR product according to the recombinant BAC template with different primers; D: Electrophoretogram of the PCR product with BamH I or AflI I digestion. M: Marker; OB: Original BAC; RB: Recombinant BAC; DkB: Deleted kanamycin BAC; 5F: Forward primer of the 5arm; 5R: Reverse primer of the 5arm; 3F: Forward primer of the 3arm; 3R: Reverse primer of the 3arm; Cre: Cre primer.

    Article Snippet: The PCR product was digested with BamH I and AflI I (New England Biolabs) for further confirmation.

    Techniques: Recombinant, Clone Assay, Polymerase Chain Reaction, BAC Assay, Marker

    Construction and quantitation of rKSHV.294. (A) Schematic diagram of rKSHV.294 showing the insertion site in the KSHV genome; the BamHI sites flanking the 4.8-kb segment of the KSHV genome used are indicated. The relative positions of the SeAP, the GFP, and the Neo elements are shown with their respective promoters but not to scale. Beneath the 294 construct are shown the expected PCR products (a to e) with the primers used for analysis of viral DNA. (B) Hybridization analysis of rKSHV.294 and JSC-1 viral DNA. (Left) KSHV probe using the 4.8-kb BamHI fragment used to construct the virus. (Right) Neo probe. Lane 1, rKSHV.294 × AflII, predicted fragments of 15.9, 5.7, and 4.8 kb. The 4.8-kb fragment contains the SeAP/GFP/Neo insert with only 170 bp of KSHV DNA, which accounts for the weak band. Lane 2, JSC-1 × AflII, predicted fragments of 15.9 and 5.8 kb. Lane 3, rKSHV.294 × SspI, predicted fragments of 8 and 6.2 kb. Lane 4, JSC-1 × SspI, predicted fragments of 6.2 and 3.3 kb. Lane 5, rKSHV.294 × AflII, predicted fragment of 4.8 kb. Lane 6, JSC-1 × AflII. Lane 7, rKSHV.294 DNA × SspI, predicted fragment of 8 kb. Lane 8, JSC-1 × SspI. DNA markers in kb are on the left side. (C) Ethidium bromide-stained agarose gel following electrophoresis of the PCR products resulting with rKSHV.294 viral DNA with the indicated primers described in Materials and Methods. Lane a, ORF56f (outside BamHI site) and SeAPr; lane b, ORF57u and SeAPr; lane c, SeAPf and GFPf; lane d, GFPr and Neor; lane e, Neof and K9. (D) rKSHV.294 was tested for tTA control of SeAP expression by the infection of 293 cells or of 293 cells expressing tTA at an MOI of

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: The HIV Protease Inhibitor Nelfinavir Inhibits Kaposi's Sarcoma-Associated Herpesvirus Replication In Vitro ▿

    doi: 10.1128/AAC.01295-10

    Figure Lengend Snippet: Construction and quantitation of rKSHV.294. (A) Schematic diagram of rKSHV.294 showing the insertion site in the KSHV genome; the BamHI sites flanking the 4.8-kb segment of the KSHV genome used are indicated. The relative positions of the SeAP, the GFP, and the Neo elements are shown with their respective promoters but not to scale. Beneath the 294 construct are shown the expected PCR products (a to e) with the primers used for analysis of viral DNA. (B) Hybridization analysis of rKSHV.294 and JSC-1 viral DNA. (Left) KSHV probe using the 4.8-kb BamHI fragment used to construct the virus. (Right) Neo probe. Lane 1, rKSHV.294 × AflII, predicted fragments of 15.9, 5.7, and 4.8 kb. The 4.8-kb fragment contains the SeAP/GFP/Neo insert with only 170 bp of KSHV DNA, which accounts for the weak band. Lane 2, JSC-1 × AflII, predicted fragments of 15.9 and 5.8 kb. Lane 3, rKSHV.294 × SspI, predicted fragments of 8 and 6.2 kb. Lane 4, JSC-1 × SspI, predicted fragments of 6.2 and 3.3 kb. Lane 5, rKSHV.294 × AflII, predicted fragment of 4.8 kb. Lane 6, JSC-1 × AflII. Lane 7, rKSHV.294 DNA × SspI, predicted fragment of 8 kb. Lane 8, JSC-1 × SspI. DNA markers in kb are on the left side. (C) Ethidium bromide-stained agarose gel following electrophoresis of the PCR products resulting with rKSHV.294 viral DNA with the indicated primers described in Materials and Methods. Lane a, ORF56f (outside BamHI site) and SeAPr; lane b, ORF57u and SeAPr; lane c, SeAPf and GFPf; lane d, GFPr and Neor; lane e, Neof and K9. (D) rKSHV.294 was tested for tTA control of SeAP expression by the infection of 293 cells or of 293 cells expressing tTA at an MOI of

    Article Snippet: Twenty micrograms of each DNA sample was digested overnight at 37°C with either AflII-HF or SspI-HF restriction endonucleases according to the manufacturer's recommendations (New England BioLabs, Ipswich, MA).

    Techniques: Quantitation Assay, Construct, Polymerase Chain Reaction, Hybridization, Staining, Agarose Gel Electrophoresis, Electrophoresis, Expressing, Infection