afl iii  (New England Biolabs)


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  • 94
    Name:
    AflIII
    Description:
    AflIII 1 250 units
    Catalog Number:
    r0541l
    Price:
    282
    Size:
    1 250 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs afl iii
    AflIII
    AflIII 1 250 units
    https://www.bioz.com/result/afl iii/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    afl iii - by Bioz Stars, 2020-10
    94/100 stars

    Images

    1) Product Images from "Correlation of Molecular Markers, Pfmdr1-N86Y and Pfcrt-K76T, with In Vitro Chloroquine Resistant Plasmodium falciparum, Isolated in the Malaria Endemic States of Assam and Arunachal Pradesh, Northeast India"

    Article Title: Correlation of Molecular Markers, Pfmdr1-N86Y and Pfcrt-K76T, with In Vitro Chloroquine Resistant Plasmodium falciparum, Isolated in the Malaria Endemic States of Assam and Arunachal Pradesh, Northeast India

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0103848

    Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (86Y) in chloroquine resistant Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (R1- R4) are chloroquine resistant P. falciparum isolates.
    Figure Legend Snippet: Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (86Y) in chloroquine resistant Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (R1- R4) are chloroquine resistant P. falciparum isolates.

    Techniques Used: Polymerase Chain Reaction, Amplification

    Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (N86) in chloroquine sensitive Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (ST1-ST4) are chloroquine sensitive P. falciparum isolates.
    Figure Legend Snippet: Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (N86) in chloroquine sensitive Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (ST1-ST4) are chloroquine sensitive P. falciparum isolates.

    Techniques Used: Polymerase Chain Reaction, Amplification

    2) Product Images from "Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells"

    Article Title: Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.251383598

    Flow diagram of RCA in situ . Pretreatment prior to in situ RCA detection of the Tp53 gene. Restriction enzymes were used to cut ≈20 base pairs either 3′ or 5′ of the probe binding site. Afl III was used for digestion 5′ of the binding site, and Bbs I was used for digestion 3′ of the target. Cells were then treated with exonuclease III, which digests DNA 3′ → 5′ starting with 3′ hydroxyl left by the endonuclease, resulting in staggered single-stranded DNA. The DNA strand remaining following Afl III digestion in this case is the complement to the sense probe sequence. DNA remaining following Bbs I digestion is the complement to the antisense probe sequence. Bbs I digestion constitutes a negative control for the RCA process using the sense probe, and Afl III constitutes a negative control for the antisense probe. The two ends of the sense probe create an incomplete circle as they anneal to the complementary site on the DNA digested with Bbs I. The DNA strand digested with Afl III is complementary to the sense probe and allows it to anneal and ligate, completing the circle and locking the probe onto the target. Targets other than Tp53 may require different endonucleases.
    Figure Legend Snippet: Flow diagram of RCA in situ . Pretreatment prior to in situ RCA detection of the Tp53 gene. Restriction enzymes were used to cut ≈20 base pairs either 3′ or 5′ of the probe binding site. Afl III was used for digestion 5′ of the binding site, and Bbs I was used for digestion 3′ of the target. Cells were then treated with exonuclease III, which digests DNA 3′ → 5′ starting with 3′ hydroxyl left by the endonuclease, resulting in staggered single-stranded DNA. The DNA strand remaining following Afl III digestion in this case is the complement to the sense probe sequence. DNA remaining following Bbs I digestion is the complement to the antisense probe sequence. Bbs I digestion constitutes a negative control for the RCA process using the sense probe, and Afl III constitutes a negative control for the antisense probe. The two ends of the sense probe create an incomplete circle as they anneal to the complementary site on the DNA digested with Bbs I. The DNA strand digested with Afl III is complementary to the sense probe and allows it to anneal and ligate, completing the circle and locking the probe onto the target. Targets other than Tp53 may require different endonucleases.

    Techniques Used: Flow Cytometry, In Situ, Binding Assay, Sequencing, Negative Control

    3) Product Images from "Correlation of Molecular Markers, Pfmdr1-N86Y and Pfcrt-K76T, with In Vitro Chloroquine Resistant Plasmodium falciparum, Isolated in the Malaria Endemic States of Assam and Arunachal Pradesh, Northeast India"

    Article Title: Correlation of Molecular Markers, Pfmdr1-N86Y and Pfcrt-K76T, with In Vitro Chloroquine Resistant Plasmodium falciparum, Isolated in the Malaria Endemic States of Assam and Arunachal Pradesh, Northeast India

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0103848

    Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (86Y) in chloroquine resistant Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (R1- R4) are chloroquine resistant P. falciparum isolates.
    Figure Legend Snippet: Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (86Y) in chloroquine resistant Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (R1- R4) are chloroquine resistant P. falciparum isolates.

    Techniques Used: Polymerase Chain Reaction, Amplification

    Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (N86) in chloroquine sensitive Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (ST1-ST4) are chloroquine sensitive P. falciparum isolates.
    Figure Legend Snippet: Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (N86) in chloroquine sensitive Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (ST1-ST4) are chloroquine sensitive P. falciparum isolates.

    Techniques Used: Polymerase Chain Reaction, Amplification

    Related Articles

    Amplification:

    Article Title: Correlation of Molecular Markers, Pfmdr1-N86Y and Pfcrt-K76T, with In Vitro Chloroquine Resistant Plasmodium falciparum, Isolated in the Malaria Endemic States of Assam and Arunachal Pradesh, Northeast India
    Article Snippet: .. Restriction Digestion with ApoI and Afl III The finally amplified product was subjected to restriction digestion with Afl III (mutational allele) and Apo I (wild type allele) (New England Biolabs, UK) by incubating at 37°C for one hour with the one unit of each enzyme. .. The digests were resolved on 3% agarose gel, stained with ethidium bromide, and results were recorded on the gel documentation system (UVITEC, UK).

    DNA Synthesis:

    Article Title: The mechanism of DNA replication termination in vertebrates
    Article Snippet: .. To monitor DNA synthesis within a lacO array , 0.25–1.0 ng/µl of purified DNA was incubated in Buffer 3.1 with 0.2 units/µl PvuII and 0.2 units/µl AflIII (New England BioLabs) at 37°C for 1 hour. ..

    Southern Blot:

    Article Title: Development and validation of a multiplex-PCR assay for X-linked intellectual disability
    Article Snippet: .. Southern blot genotyping was done using ~10 μg of gDNA double-digested with the restriction enzymes EcoRI and EagI for FMR1 gene or AflIII and NotI for AFF2 gene (New England Biolabs, Ipswich, MA, USA). .. Further, the digested gDNA products were electrophoresed in parallel with a 1:1 mixture of the standard size markers, DNA Molecular Weight Marker II and III digoxigenin-labeled (Roche Applied Science, Indianapolis, IN, USA), blotted and hybridized using the FMR1 or the AFF2 digoxigenin-labeled specific probes, GLFXDIG1 or AJ31Dig1, as appropriate (Gene Link™, Hawthorne, NY, USA).

    Purification:

    Article Title: The mechanism of DNA replication termination in vertebrates
    Article Snippet: .. To monitor DNA synthesis within a lacO array , 0.25–1.0 ng/µl of purified DNA was incubated in Buffer 3.1 with 0.2 units/µl PvuII and 0.2 units/µl AflIII (New England BioLabs) at 37°C for 1 hour. ..

    Transgenic Assay:

    Article Title: Low-cost production of proinsulin in tobacco and lettuce chloroplasts for injectable or oral delivery of functional insulin and C-peptide
    Article Snippet: .. Transgenic and untransformed tobacco DNA was digested using AflIII, and lettuce DNA was digested using BglII in a reaction mixture containing 2 μL 10× buffer (New England Biolabs, Ipswich, MA), 3 μg plant genomic DNA, 2 μL BSA and 1 μL enzyme made up to 20 μL with dH2 O. .. Southern analysis was performed according to laboratory protocol ( ).

    Incubation:

    Article Title: Clinically Relevant Outcome Measures for the I307N Rhodopsin Mouse: A Model of Inducible Autosomal Dominant Retinitis Pigmentosa
    Article Snippet: .. The I307N Rho PCR-fragment, when incubated with AflIII (New England Biolabs, Ipswich, MA, USA), liberates two fragments of approximately 150 and 110 base-pairs. .. The WT Rho PCR-fragment retina retains the full 260 base-pair length.

    Article Title: The mechanism of DNA replication termination in vertebrates
    Article Snippet: .. To monitor DNA synthesis within a lacO array , 0.25–1.0 ng/µl of purified DNA was incubated in Buffer 3.1 with 0.2 units/µl PvuII and 0.2 units/µl AflIII (New England BioLabs) at 37°C for 1 hour. ..

    Polymerase Chain Reaction:

    Article Title: Clinically Relevant Outcome Measures for the I307N Rhodopsin Mouse: A Model of Inducible Autosomal Dominant Retinitis Pigmentosa
    Article Snippet: .. The I307N Rho PCR-fragment, when incubated with AflIII (New England Biolabs, Ipswich, MA, USA), liberates two fragments of approximately 150 and 110 base-pairs. .. The WT Rho PCR-fragment retina retains the full 260 base-pair length.

    Article Title: A tale of two haplotype groups: Evaluating the New World Junonia ring species hypothesis using the distribution of divergent COI haplotypes
    Article Snippet: .. PCR products of the correct size were evaluated by a triple digest using BseYI, AflIII, and BamHI restriction endonucleases (New England Biolabs, Ipswich, MA, USA) ( ). .. A 10 µL aliquot of the PCR product was incubated with 10 µL of the triple digest cocktail (2 µL of NEB Buffer3, 2 µL of BSA (10×, 1 mg/mL), 4 µL of ddH2 O, 0.5 µL of BseYI, 0.5 µL of AflIII and 1 µL of BamHI) for 1 hour in a 37°C water bath.

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  • 94
    New England Biolabs afl iii
    Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI <t>III</t> for Pfmdr1 gene (86Y) in chloroquine resistant Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is <t>Afl</t> III and (R1- R4) are chloroquine resistant P. falciparum isolates.
    Afl Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/afl iii/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    afl iii - by Bioz Stars, 2020-10
    94/100 stars
      Buy from Supplier

    Image Search Results


    Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (86Y) in chloroquine resistant Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (R1- R4) are chloroquine resistant P. falciparum isolates.

    Journal: PLoS ONE

    Article Title: Correlation of Molecular Markers, Pfmdr1-N86Y and Pfcrt-K76T, with In Vitro Chloroquine Resistant Plasmodium falciparum, Isolated in the Malaria Endemic States of Assam and Arunachal Pradesh, Northeast India

    doi: 10.1371/journal.pone.0103848

    Figure Lengend Snippet: Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (86Y) in chloroquine resistant Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (R1- R4) are chloroquine resistant P. falciparum isolates.

    Article Snippet: Restriction Digestion with ApoI and Afl III The finally amplified product was subjected to restriction digestion with Afl III (mutational allele) and Apo I (wild type allele) (New England Biolabs, UK) by incubating at 37°C for one hour with the one unit of each enzyme.

    Techniques: Polymerase Chain Reaction, Amplification

    Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (N86) in chloroquine sensitive Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (ST1-ST4) are chloroquine sensitive P. falciparum isolates.

    Journal: PLoS ONE

    Article Title: Correlation of Molecular Markers, Pfmdr1-N86Y and Pfcrt-K76T, with In Vitro Chloroquine Resistant Plasmodium falciparum, Isolated in the Malaria Endemic States of Assam and Arunachal Pradesh, Northeast India

    doi: 10.1371/journal.pone.0103848

    Figure Lengend Snippet: Representative photomicrograph showing the results of RFLP after digestion of PCR amplified product with endonucleases, Apo1 and AfI III for Pfmdr1 gene (N86) in chloroquine sensitive Plasmodium falciparum isolates. In photograph, (M) is 100 bp DNA ladder; (C) is undigested product as control; (P) is Apo1; (F) is Afl III and (ST1-ST4) are chloroquine sensitive P. falciparum isolates.

    Article Snippet: Restriction Digestion with ApoI and Afl III The finally amplified product was subjected to restriction digestion with Afl III (mutational allele) and Apo I (wild type allele) (New England Biolabs, UK) by incubating at 37°C for one hour with the one unit of each enzyme.

    Techniques: Polymerase Chain Reaction, Amplification

    Flow diagram of RCA in situ . Pretreatment prior to in situ RCA detection of the Tp53 gene. Restriction enzymes were used to cut ≈20 base pairs either 3′ or 5′ of the probe binding site. Afl III was used for digestion 5′ of the binding site, and Bbs I was used for digestion 3′ of the target. Cells were then treated with exonuclease III, which digests DNA 3′ → 5′ starting with 3′ hydroxyl left by the endonuclease, resulting in staggered single-stranded DNA. The DNA strand remaining following Afl III digestion in this case is the complement to the sense probe sequence. DNA remaining following Bbs I digestion is the complement to the antisense probe sequence. Bbs I digestion constitutes a negative control for the RCA process using the sense probe, and Afl III constitutes a negative control for the antisense probe. The two ends of the sense probe create an incomplete circle as they anneal to the complementary site on the DNA digested with Bbs I. The DNA strand digested with Afl III is complementary to the sense probe and allows it to anneal and ligate, completing the circle and locking the probe onto the target. Targets other than Tp53 may require different endonucleases.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Detection of DNA point mutations and mRNA expression levels by rolling circle amplification in individual cells

    doi: 10.1073/pnas.251383598

    Figure Lengend Snippet: Flow diagram of RCA in situ . Pretreatment prior to in situ RCA detection of the Tp53 gene. Restriction enzymes were used to cut ≈20 base pairs either 3′ or 5′ of the probe binding site. Afl III was used for digestion 5′ of the binding site, and Bbs I was used for digestion 3′ of the target. Cells were then treated with exonuclease III, which digests DNA 3′ → 5′ starting with 3′ hydroxyl left by the endonuclease, resulting in staggered single-stranded DNA. The DNA strand remaining following Afl III digestion in this case is the complement to the sense probe sequence. DNA remaining following Bbs I digestion is the complement to the antisense probe sequence. Bbs I digestion constitutes a negative control for the RCA process using the sense probe, and Afl III constitutes a negative control for the antisense probe. The two ends of the sense probe create an incomplete circle as they anneal to the complementary site on the DNA digested with Bbs I. The DNA strand digested with Afl III is complementary to the sense probe and allows it to anneal and ligate, completing the circle and locking the probe onto the target. Targets other than Tp53 may require different endonucleases.

    Article Snippet: Either Afl III or Bbs I (0.1 unit/μl, New England Biolabs) was applied for 12 h at 37°C.

    Techniques: Flow Cytometry, In Situ, Binding Assay, Sequencing, Negative Control