affinity purified goat anti mouse immunoglobulins  (Valiant)

 
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    Name:
    Anti mouse immunoglobulins IgG IgA IgM affinity purified goat antibody fluorescein conjugated
    Description:
    Product is fluorescein 5 isothiocyanate FITC Isomer I conjugated goat affinity purified antibody to mouse immunoglobulins IgG IgA IgM and buffer salts
    Catalog Number:
    0855521
    Price:
    480.6
    Category:
    Life Sciences Antibodies Secondary Antibodies
    Applications:
    Immunoassays, Immunohistochemistry (Paraffin; Acetone-Fixed; 4% PFA Frozen), Immunofluorescence assays (IFA), Flow Cytometry (FACS)
    Size:
    2 mg
    Buy from Supplier


    Structured Review

    Valiant affinity purified goat anti mouse immunoglobulins
    Anti mouse immunoglobulins IgG IgA IgM affinity purified goat antibody fluorescein conjugated
    Product is fluorescein 5 isothiocyanate FITC Isomer I conjugated goat affinity purified antibody to mouse immunoglobulins IgG IgA IgM and buffer salts
    https://www.bioz.com/result/affinity purified goat anti mouse immunoglobulins/product/Valiant
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    affinity purified goat anti mouse immunoglobulins - by Bioz Stars, 2021-05
    85/100 stars

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    Related Articles

    Incubation:

    Article Title: An alternative −1/+2 open reading frame exists within viral Npro(1–19) region of bovine viral diarrhea virus SD-1
    Article Snippet: 40 μg of cell lysates were separated on an 8–16% SDS-PAGE gel (Precise protein gel, Pierce) and transferred to a PVDF membrane (Sigma–Aldrich, South San Francisco, CA). .. The membrane was blocked for 1 h, incubated with mouse anti-GFP Mab JL-8 (1:1000 dilution, Clontech, Palo Alto, CA) for 2 h, washed with PBS followed incubation with alkaline phosphatase-conjugated, affinity purified goat anti mouse immunoglobulins (IgG, IgA, IgM) (1:1000 dilution, MP Biomedicals, Aurora, OH) for 1 h. After washing with PBS, green fluorescent protein was detected with an alkaline phosphatase substrate (Bio-Rad) for 10 min and recorded with a Canon Powershot A80 digital camera (Canon USA Inc., Lake Success, NY). .. 3.1 Rescue of an infectious reporter BVDV lacking a single nucleotide in its genome As reported previously , BSD1-Npro /eGFP2A is an engineered Npro -disrupted reporter BVDV derived from an infectious BAC cDNA clone, pBSD1-Npro /eGFP2A, with a NCP BVDV SD-1 background ( ).

    Affinity Purification:

    Article Title: An alternative −1/+2 open reading frame exists within viral Npro(1–19) region of bovine viral diarrhea virus SD-1
    Article Snippet: 40 μg of cell lysates were separated on an 8–16% SDS-PAGE gel (Precise protein gel, Pierce) and transferred to a PVDF membrane (Sigma–Aldrich, South San Francisco, CA). .. The membrane was blocked for 1 h, incubated with mouse anti-GFP Mab JL-8 (1:1000 dilution, Clontech, Palo Alto, CA) for 2 h, washed with PBS followed incubation with alkaline phosphatase-conjugated, affinity purified goat anti mouse immunoglobulins (IgG, IgA, IgM) (1:1000 dilution, MP Biomedicals, Aurora, OH) for 1 h. After washing with PBS, green fluorescent protein was detected with an alkaline phosphatase substrate (Bio-Rad) for 10 min and recorded with a Canon Powershot A80 digital camera (Canon USA Inc., Lake Success, NY). .. 3.1 Rescue of an infectious reporter BVDV lacking a single nucleotide in its genome As reported previously , BSD1-Npro /eGFP2A is an engineered Npro -disrupted reporter BVDV derived from an infectious BAC cDNA clone, pBSD1-Npro /eGFP2A, with a NCP BVDV SD-1 background ( ).

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  • 91
    Valiant peroxidase conjugated goat anti mouse igg
    Analysis of the expressed recombinant GST-tagged FIPV KU-2 nucleocapsid protein using western immunoblotting. <t>F19-1,</t> anti-FCoV M MAb <t>(IgG1);</t> E22-2, anti-FCoV N MAb (IgG1).
    Peroxidase Conjugated Goat Anti Mouse Igg, supplied by Valiant, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/peroxidase conjugated goat anti mouse igg/product/Valiant
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    peroxidase conjugated goat anti mouse igg - by Bioz Stars, 2021-05
    91/100 stars
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    95
    Valiant anti hamster igg whole molecule goat affinity purified antibody
    PPP2R2D deficiency in T cells mitigates imiquimod-induced lupus-like pathology in mice. Topical imiquimod was applied to the ear skin of R2D fl/fl and Lck Cre R2D fl/fl mice ( n = 7/group) for 8 weeks. ( A ) The representative picture and cumulative data of the weight of spleens. FACS analysis of the percentage of <t>CD3</t> + CD4 + IFN-γ + ( B ), CD3 + CD4 + IL-17A + ( C ) , CD3 + CD4 + IL-2 + ( D ), and CD3 + CD4 + FoxP3 + ( E ) cells in spleens. ( F and G ) The expression levels of Treg markers CTLA-4 ( F ) and GITR ( G ) in splenic Tregs (CD3 + CD4 + FoxP3 + ) were determined by FACS. ( H and I ) The anti-dsDNA <t>IgG</t> level in serum ( H ) and the levels of albumin and creatinine in urine ( I ) were measured by ELISA. ( J and K ) The deposition of complement 3 (C3) and IgG in glomeruli was determined by immunofluorescence staining. Representative figures ( J ) and cumulative data ( K ) depicting numbers of glomeruli with C3 and IgG double deposition in coronal sections of kidney. Scale bar: 50 μm. ( L ) Representative H E staining of kidney tissues. Scale bar: 20 μm. ( M ) Cumulative data elucidating the histopathologic scores for glomerulonephritis. ( N ) Kidney-infiltrating lymphocytes including total T cells (Thy1.2), CD4 + T cells, and CD8 + T cells were counted and analyzed by FACS. * P
    Anti Hamster Igg Whole Molecule Goat Affinity Purified Antibody, supplied by Valiant, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti hamster igg whole molecule goat affinity purified antibody/product/Valiant
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti hamster igg whole molecule goat affinity purified antibody - by Bioz Stars, 2021-05
    95/100 stars
      Buy from Supplier

    92
    Valiant higg
    Catabolism of <t>hIgG</t> in <t>TG32B</t> mice. Human IgG was injected i.v. at t = 0, followed by i.v. injections of test article at t = 24, 28, 72, and 96 h. Test article: vehicle (diamonds), 10 mg/kg SYN1327 (filled circles), and SYN1436 at 0.5 mg/kg (open circles),
    Higg, supplied by Valiant, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/higg/product/Valiant
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    higg - by Bioz Stars, 2021-05
    92/100 stars
      Buy from Supplier

    91
    Valiant anti igm
    Abrogation of signaling downstream of the BCRs and of CXCR4 and CXCR5 receptors. Displayed are immunoblots from <t>CLL</t> cells from 1 representative patient of 9 patients, that were either unstimulated or stimulated for 10 minutes with <t>anti-IgM</t> (αIgM,
    Anti Igm, supplied by Valiant, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti igm/product/Valiant
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti igm - by Bioz Stars, 2021-05
    91/100 stars
      Buy from Supplier

    Image Search Results


    Analysis of the expressed recombinant GST-tagged FIPV KU-2 nucleocapsid protein using western immunoblotting. F19-1, anti-FCoV M MAb (IgG1); E22-2, anti-FCoV N MAb (IgG1).

    Journal: Journal of Virological Methods

    Article Title: Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests

    doi: 10.1016/j.jviromet.2013.10.014

    Figure Lengend Snippet: Analysis of the expressed recombinant GST-tagged FIPV KU-2 nucleocapsid protein using western immunoblotting. F19-1, anti-FCoV M MAb (IgG1); E22-2, anti-FCoV N MAb (IgG1).

    Article Snippet: The membrane was blocked with 5% non-fat dry milk powder in TBST (20 mM Tris–HCl, 150 mM sodium chloride, and 0.05%Tween-20, pH 8.0) for 1 h at 37 °C, incubated for 1 h at 37 °C with MAb F19-1 or E22-2, and then incubated with peroxidase-conjugated goat anti-mouse IgG (MP Biomedicals, LLC-Cappel products, CA, USA) for 1 h at 37 °C.

    Techniques: Recombinant, Western Blot

    PPP2R2D deficiency in T cells mitigates imiquimod-induced lupus-like pathology in mice. Topical imiquimod was applied to the ear skin of R2D fl/fl and Lck Cre R2D fl/fl mice ( n = 7/group) for 8 weeks. ( A ) The representative picture and cumulative data of the weight of spleens. FACS analysis of the percentage of CD3 + CD4 + IFN-γ + ( B ), CD3 + CD4 + IL-17A + ( C ) , CD3 + CD4 + IL-2 + ( D ), and CD3 + CD4 + FoxP3 + ( E ) cells in spleens. ( F and G ) The expression levels of Treg markers CTLA-4 ( F ) and GITR ( G ) in splenic Tregs (CD3 + CD4 + FoxP3 + ) were determined by FACS. ( H and I ) The anti-dsDNA IgG level in serum ( H ) and the levels of albumin and creatinine in urine ( I ) were measured by ELISA. ( J and K ) The deposition of complement 3 (C3) and IgG in glomeruli was determined by immunofluorescence staining. Representative figures ( J ) and cumulative data ( K ) depicting numbers of glomeruli with C3 and IgG double deposition in coronal sections of kidney. Scale bar: 50 μm. ( L ) Representative H E staining of kidney tissues. Scale bar: 20 μm. ( M ) Cumulative data elucidating the histopathologic scores for glomerulonephritis. ( N ) Kidney-infiltrating lymphocytes including total T cells (Thy1.2), CD4 + T cells, and CD8 + T cells were counted and analyzed by FACS. * P

    Journal: JCI Insight

    Article Title: PPP2R2D suppresses IL-2 production and Treg function

    doi: 10.1172/jci.insight.138215

    Figure Lengend Snippet: PPP2R2D deficiency in T cells mitigates imiquimod-induced lupus-like pathology in mice. Topical imiquimod was applied to the ear skin of R2D fl/fl and Lck Cre R2D fl/fl mice ( n = 7/group) for 8 weeks. ( A ) The representative picture and cumulative data of the weight of spleens. FACS analysis of the percentage of CD3 + CD4 + IFN-γ + ( B ), CD3 + CD4 + IL-17A + ( C ) , CD3 + CD4 + IL-2 + ( D ), and CD3 + CD4 + FoxP3 + ( E ) cells in spleens. ( F and G ) The expression levels of Treg markers CTLA-4 ( F ) and GITR ( G ) in splenic Tregs (CD3 + CD4 + FoxP3 + ) were determined by FACS. ( H and I ) The anti-dsDNA IgG level in serum ( H ) and the levels of albumin and creatinine in urine ( I ) were measured by ELISA. ( J and K ) The deposition of complement 3 (C3) and IgG in glomeruli was determined by immunofluorescence staining. Representative figures ( J ) and cumulative data ( K ) depicting numbers of glomeruli with C3 and IgG double deposition in coronal sections of kidney. Scale bar: 50 μm. ( L ) Representative H E staining of kidney tissues. Scale bar: 20 μm. ( M ) Cumulative data elucidating the histopathologic scores for glomerulonephritis. ( N ) Kidney-infiltrating lymphocytes including total T cells (Thy1.2), CD4 + T cells, and CD8 + T cells were counted and analyzed by FACS. * P

    Article Snippet: Purified naive T cells were stimulated with plate-bound goat anti–hamster IgG (SKU 0856984, MP Biomedicals), soluble anti-CD3 (0.25 μg/mL,145-2C11; BioLegend), and anti-CD28 (0.5 μg/mL, 37.51; BioLegend) for Th0-nonpolarized condition culture.

    Techniques: Mouse Assay, FACS, Expressing, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Staining

    Catabolism of hIgG in TG32B mice. Human IgG was injected i.v. at t = 0, followed by i.v. injections of test article at t = 24, 28, 72, and 96 h. Test article: vehicle (diamonds), 10 mg/kg SYN1327 (filled circles), and SYN1436 at 0.5 mg/kg (open circles),

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Reduction of IgG in nonhuman primates by a peptide antagonist of the neonatal Fc receptor FcRn

    doi: 10.1073/pnas.0708960105

    Figure Lengend Snippet: Catabolism of hIgG in TG32B mice. Human IgG was injected i.v. at t = 0, followed by i.v. injections of test article at t = 24, 28, 72, and 96 h. Test article: vehicle (diamonds), 10 mg/kg SYN1327 (filled circles), and SYN1436 at 0.5 mg/kg (open circles),

    Article Snippet: TG32B mice (four animals per group) were injected i.v. with 500 mg/kg hIgG (pooled IgG subtypes; MP Biomedicals) at t = 0 h. The mice were then injected i.v. at t = 24, 48, 72, and 96 h with vehicle, 10 mg/kg of SYN1327, or SYN1436 at doses of 0.5, 1, 2.5, 5, or 10 mg/kg.

    Techniques: Mouse Assay, Injection

    Abrogation of signaling downstream of the BCRs and of CXCR4 and CXCR5 receptors. Displayed are immunoblots from CLL cells from 1 representative patient of 9 patients, that were either unstimulated or stimulated for 10 minutes with anti-IgM (αIgM,

    Journal: Blood

    Article Title: The Bruton tyrosine kinase inhibitor PCI-32765 thwarts chronic lymphocytic leukemia cell survival and tissue homing in vitro and in vivo

    doi: 10.1182/blood-2011-10-386417

    Figure Lengend Snippet: Abrogation of signaling downstream of the BCRs and of CXCR4 and CXCR5 receptors. Displayed are immunoblots from CLL cells from 1 representative patient of 9 patients, that were either unstimulated or stimulated for 10 minutes with anti-IgM (αIgM,

    Article Snippet: To determine the efficacy of the inhibitor to antagonize BCR-derived prosurvival signals after BCR triggering with anti-IgM, CLL samples (107 cells/mL) were preincubated in complete RPMI medium with or without PCI-32765 for 30 minutes at 37°C, and then stimulated by the addition of 10 μg/mL anti-IgM (polyclonal goat F(ab′)2 fragments to human IgM; MP Biomedicals).

    Techniques: Western Blot

    PCI-32765 inhibits anti-IgM and NLC-mediated prosurvival signals in CLL cells. (A) Contour plots of a representative CLL sample, depicting CLL cell viabilities after 24, 48, and 72 hours of incubation with anti-IgM in the presence or absence of PCI-32765,

    Journal: Blood

    Article Title: The Bruton tyrosine kinase inhibitor PCI-32765 thwarts chronic lymphocytic leukemia cell survival and tissue homing in vitro and in vivo

    doi: 10.1182/blood-2011-10-386417

    Figure Lengend Snippet: PCI-32765 inhibits anti-IgM and NLC-mediated prosurvival signals in CLL cells. (A) Contour plots of a representative CLL sample, depicting CLL cell viabilities after 24, 48, and 72 hours of incubation with anti-IgM in the presence or absence of PCI-32765,

    Article Snippet: To determine the efficacy of the inhibitor to antagonize BCR-derived prosurvival signals after BCR triggering with anti-IgM, CLL samples (107 cells/mL) were preincubated in complete RPMI medium with or without PCI-32765 for 30 minutes at 37°C, and then stimulated by the addition of 10 μg/mL anti-IgM (polyclonal goat F(ab′)2 fragments to human IgM; MP Biomedicals).

    Techniques: Incubation