affinity nickel nta agarose resin  (Gold Biotechnology Inc)


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    Gold Biotechnology Inc affinity nickel nta agarose resin
    Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from <t>NTA-His</t> column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.
    Affinity Nickel Nta Agarose Resin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity nickel nta agarose resin/product/Gold Biotechnology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    affinity nickel nta agarose resin - by Bioz Stars, 2022-12
    94/100 stars

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    1) Product Images from "Optimization and validation of a Protein Phosphatase inhibition assay for accessible microcystin detection"

    Article Title: Optimization and validation of a Protein Phosphatase inhibition assay for accessible microcystin detection

    Journal: bioRxiv

    doi: 10.1101/2022.08.16.502937

    Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.
    Figure Legend Snippet: Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.

    Techniques Used: Expressing, SDS Page, Protein Purification, Marker, Purification, Inhibition, Activity Assay

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  • 94
    Gold Biotechnology Inc affinity nickel nta agarose resin
    Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from <t>NTA-His</t> column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.
    Affinity Nickel Nta Agarose Resin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/affinity nickel nta agarose resin/product/Gold Biotechnology Inc
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    affinity nickel nta agarose resin - by Bioz Stars, 2022-12
    94/100 stars
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    Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.

    Journal: bioRxiv

    Article Title: Optimization and validation of a Protein Phosphatase inhibition assay for accessible microcystin detection

    doi: 10.1101/2022.08.16.502937

    Figure Lengend Snippet: Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.

    Article Snippet: Protein was purified in the same buffer with lower ionic strength (70 mM NaCl instead), through an affinity Nickel-NTA Agarose Resin (Gold Biotechnology, Missouri, United States) column by gravity.

    Techniques: Expressing, SDS Page, Protein Purification, Marker, Purification, Inhibition, Activity Assay