Affinity Nickel Nta Agarose Resin, supplied by Gold Biotechnology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Optimization and validation of a Protein Phosphatase inhibition assay for accessible microcystin detection"
Article Title: Optimization and validation of a Protein Phosphatase inhibition assay for accessible microcystin detection
Figure Legend Snippet: Expression, characterization and storage of PP1. A. SDS-PAGE showing protein purification after eluting from NTA-His column, against molecular marker ( NEB 1 Kb ) Left: Chaperones coeluted with PP1. Right: PP1 was purified from chaperones due to extensive washing with 20mM Imidazole. B. Inhibition profile displacement while diluting protein from 62.5 μg/mL (filled circles and thick line) in six exponential half dilutions down to 1.95 μg/mL (empty square and dotted line). C. Phosphatase activity decayes in time for four storage conditions. The left dark-gray bar of each treatment corresponds to t=0. The following gray bars correspond to weeks 1-4. Error bars correspond to SEM.
Techniques Used: Expressing, SDS Page, Protein Purification, Marker, Purification, Inhibition, Activity Assay