afb1 solution  (Millipore)


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  • 95
    Name:
    Aflatoxin B1 solution
    Description:
    Aflatoxin B1 AFB1 is a carcinogenic compound that induces transversion of G to T at codon 249 of the p53 tumor suppressor gene AFB1 is the most common in food and amongst the most potent genotoxic and carcinogenic aflatoxins It is produced by both Aspergillus flavus and A parasiticus
    Catalog Number:
    46323-u
    Price:
    None
    Applications:
    AFB1 solution was used as reference standard for the analysis of mycotoxins in paprika using LC-FD and LC-MS. AFB1 may be used as spiking standard solution to compare and determine AFB1 in corn and peanut samples using ultra-high-pressure liquid chromatography with UV detection.
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    Structured Review

    Millipore afb1 solution
    Aflatoxin B1 solution
    Aflatoxin B1 AFB1 is a carcinogenic compound that induces transversion of G to T at codon 249 of the p53 tumor suppressor gene AFB1 is the most common in food and amongst the most potent genotoxic and carcinogenic aflatoxins It is produced by both Aspergillus flavus and A parasiticus
    https://www.bioz.com/result/afb1 solution/product/Millipore
    Average 95 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    afb1 solution - by Bioz Stars, 2020-09
    95/100 stars

    Related Products / Commonly Used Together

    candidate isolates
    afb1
    fermented supernatant

    Images

    Related Articles

    Concentration Assay:

    Article Title: Aflatoxin B1 Degradation by Stenotrophomonas Maltophilia and Other Microbes Selected Using Coumarin Medium #
    Article Snippet: .. AFB1 standard solution (Sigma Chemical Co., Bellefonte, USA) was diluted with methanol (Beijing Chemical Inc., Beijing, China) to a stock solution of 500 ppb, of which 0.2 mL was added to microbial cultures of 0.8 mL for a final concentration of 100 ppb. .. The degradation tests were conducted in the dark at 37 °C without shaking for 72 h. After incubation, cells of microbes were removed by centrifugation at 10,000 g for 10 min (Beijing Medical Centrifugator Inc., China).

    Article Title: Hepatic Transcriptome Responses of Domesticated and Wild Turkey Embryos to Aflatoxin B1
    Article Snippet: .. Throughout the experiment, eggs were incubated at 37.0 ± 0.5 °C with approximately 40% humidity and rotation every 2 h. AFB1 -solution was made by directly suspending AFB1 (Sigma-Aldrich, St. Louis, MO, USA) in 100% EtOH and diluting to a final concentration of 5 μg/mL in 30% EtOH. .. In ovo AFB1 Exposure Eggs were candled prior to the start of the exposure period to verify viability.

    Article Title: In Vitro Efficacy of Myxococcus fulvus ANSM068 to Biotransform Aflatoxin B1
    Article Snippet: .. AFB1 standard solution (Sigma Chemical Co., Bellefonte, USA) was diluted with methanol (Beijing Chemical Inc., Beijing, China) to a stock solution of 500 ppb, of which 0.2 mL was added to bacterial cultures of 0.8 mL for a final concentration of 100 ppb. .. The biotransformation tests were conducted in the dark at 30 °C without shaking for 72 h. After incubation, bacterial cells were removed by centrifugation at 9,300 g for 10 min (TGL-16C centrifuge, Beijing Medical Centrifuge Inc., China).

    Incubation:

    Article Title: Hepatic Transcriptome Responses of Domesticated and Wild Turkey Embryos to Aflatoxin B1
    Article Snippet: .. Throughout the experiment, eggs were incubated at 37.0 ± 0.5 °C with approximately 40% humidity and rotation every 2 h. AFB1 -solution was made by directly suspending AFB1 (Sigma-Aldrich, St. Louis, MO, USA) in 100% EtOH and diluting to a final concentration of 5 μg/mL in 30% EtOH. .. In ovo AFB1 Exposure Eggs were candled prior to the start of the exposure period to verify viability.

    other:

    Article Title: Immobilized Saccharomyces Cerevisiae as a potential aflatoxin decontaminating agent in pistachio nuts
    Article Snippet: Chemicals and yeast Aflatoxin B1 solution was purchased from Sigma Co. (St. Louis, MO, USA).

    Cell Culture:

    Article Title: A novel strain of Cellulosimicrobium funkei can biologically detoxify aflatoxin B1 in ducklings
    Article Snippet: .. Initially, candidate isolates were cultured at 37°C in LB medium for 72 h, and then 950 μl fermented supernatant was taken and mixed with 50 μl 10 μg/ml AFB1 solution (Sigma Chemical Co., Bellefonte, USA) in a sterilized centrifuge tube, and then biodegradation tests were conducted at 37°C for 72 h. Finally, the reaction solution was centrifuged at 10 000 g at 4°C for 10 min to remove cells and the supernatant, and then it was collected for AFB1 quantification (Guan et al ., ). .. The AFB1 concentration was determined by HPLC (Teniola et al ., ) with a minor modification.

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    Millipore pronase
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    Millipore γ h2ax antibody millipore
    Genome instability generated by AFB 1 exposures in pol ζ-deficient cells. ( A ) DSB formation was assessed by measuring the accumulation and resolution of <t>γ-H2AX</t> foci in response to 200 nM AFB 1 +S9 for 1.5 h. Representative images of γ-H2AX
    γ H2ax Antibody Millipore, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore mtesr1
    Generation, self-renewal, and differentiation of hiHSCs. Generation, self-renewal, and differentiation of hiHSCs were schematically illustrated. hiHSCs were generated and expanded as a new type of hiPSC from human somatic cells by gene transfer of defined factors and with some modifications of their culture conditions. The autonomous hepatic specification of hiHSCs was due to their culture conditions (coculture with MEF in <t>mTeSR1</t> medium at a very high density) in self-renewal rather than in differentiation. Self-renewing hiHSCs expressed markers of both hESCs and hepatocytes and then autonomously differentiated to hepatocyte-like cells in a defined minimum medium without FGF-2 and TGF-β1in vitro. Otherwise, in vivo differentiated hiHSCs gave rise not only to hepatocyte-like cells but also to other multi-lineage cells and then secreted human ALB in mice.
    Mtesr1, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mtesr1/product/Millipore
    Average 93 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    mtesr1 - by Bioz Stars, 2020-09
    93/100 stars
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    Image Search Results


    Genome instability generated by AFB 1 exposures in pol ζ-deficient cells. ( A ) DSB formation was assessed by measuring the accumulation and resolution of γ-H2AX foci in response to 200 nM AFB 1 +S9 for 1.5 h. Representative images of γ-H2AX

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: DNA polymerase ζ limits chromosomal damage and promotes cell survival following aflatoxin exposure

    doi: 10.1073/pnas.1609024113

    Figure Lengend Snippet: Genome instability generated by AFB 1 exposures in pol ζ-deficient cells. ( A ) DSB formation was assessed by measuring the accumulation and resolution of γ-H2AX foci in response to 200 nM AFB 1 +S9 for 1.5 h. Representative images of γ-H2AX

    Article Snippet: The materials, reagents, and kits were purchased from the following suppliers: AFB1 , NADPH, and BrdU—Sigma-Aldrich; aroclor-induced rat liver microsome S9—Moltox; CellTiter-Glo luminescent cell viability assay (CTG)—Promega; Annexin V apoptosis detection kit and anti-BrdU antibody—eBioscience; γ-H2AX antibody—Millipore; media and reagents for cell culture, PI, and DNase I (Amplification Grade)—Life Technologies; FBS—Atlanta Biologicals; oligodeoxynucleotides—Integrated DNA Technologies; HiPerFect transfection reagent and RNeasy Mini kit—Qiagen; iScript cDNA synthesis kit— Bio-Rad; SYBR Green Supermix and 594-conjugated donkey α-mouse secondary antibody—Invitrogen; restriction endonucleases and S1 nuclease—New England Biolabs; and all other chemicals used for these experiments—Sigma-Aldrich or Fisher Scientific.

    Techniques: Generated, Ziehl-Neelsen Stain

    Generation, self-renewal, and differentiation of hiHSCs. Generation, self-renewal, and differentiation of hiHSCs were schematically illustrated. hiHSCs were generated and expanded as a new type of hiPSC from human somatic cells by gene transfer of defined factors and with some modifications of their culture conditions. The autonomous hepatic specification of hiHSCs was due to their culture conditions (coculture with MEF in mTeSR1 medium at a very high density) in self-renewal rather than in differentiation. Self-renewing hiHSCs expressed markers of both hESCs and hepatocytes and then autonomously differentiated to hepatocyte-like cells in a defined minimum medium without FGF-2 and TGF-β1in vitro. Otherwise, in vivo differentiated hiHSCs gave rise not only to hepatocyte-like cells but also to other multi-lineage cells and then secreted human ALB in mice.

    Journal: PLoS ONE

    Article Title: Human Induced Hepatic Lineage-Oriented Stem Cells: Autonomous Specification of Human iPS Cells toward Hepatocyte-Like Cells without Any Exogenous Differentiation Factors

    doi: 10.1371/journal.pone.0123193

    Figure Lengend Snippet: Generation, self-renewal, and differentiation of hiHSCs. Generation, self-renewal, and differentiation of hiHSCs were schematically illustrated. hiHSCs were generated and expanded as a new type of hiPSC from human somatic cells by gene transfer of defined factors and with some modifications of their culture conditions. The autonomous hepatic specification of hiHSCs was due to their culture conditions (coculture with MEF in mTeSR1 medium at a very high density) in self-renewal rather than in differentiation. Self-renewing hiHSCs expressed markers of both hESCs and hepatocytes and then autonomously differentiated to hepatocyte-like cells in a defined minimum medium without FGF-2 and TGF-β1in vitro. Otherwise, in vivo differentiated hiHSCs gave rise not only to hepatocyte-like cells but also to other multi-lineage cells and then secreted human ALB in mice.

    Article Snippet: The expanded clones AFB1-1, NGC1-1, and NGC1-2 were treated with a dissociation solution (0.25% trypsin—EDTA [Gibco] and 1% collagenase [Invitrogen]) or 0.25% trypsin—EDTA and passaged in mTeSR1 supplemented with 10–20 μM Y-27632 (Calbiochem and Wako) to avoid cell death.

    Techniques: Generated, In Vivo, Mouse Assay