Journal: Scientific Reports

Article Title: Deciphering the interaction between N -palmitoyl-D-glucosamine and the endocannabinoidome

doi: 10.1038/s41598-025-07103-5

Figure Lengend Snippet: Effect of PGA on the endogenous levels of AEA, 2-AG and PEA in HaCaT cells. A-C Concentrations of AEA, 2-AG and PEA in HaCaT cells treated with PGA (10 µM), or Vehicle for 40 min. Student’s t test was used for analysis. *** P < 0.001 and **** P < 0.0001: Vehicle vs. PGA. D-F Concentrations of AEA, 2-AG and PEA in HaCaT cells treated with PGA (10 µM), or Vehicle for 6 h. Student’s t test was used for analysis. * P < 0.05 and ** P < 0.01: Vehicle vs. PGA.

Article Snippet: Deuterated standards—[ 2 H] 8 -AEA, [ 2 H] 5 −2-AG and [ 2 H] 4 -PEA—were purchased from Cayman Chemical (Cabru, Arcore, Italy).

Techniques:

Journal: Scientific Reports

Article Title: Deciphering the interaction between N -palmitoyl-D-glucosamine and the endocannabinoidome

doi: 10.1038/s41598-025-07103-5

Figure Lengend Snippet: Effect of PGA on the endogenous levels of AEA, 2-AG and PEA in SH-SY5Y and RBL-2H3 cells. A-C Concentrations of AEA, 2-AG and PEA in SH-SY5Y cells treated with PGA (10 µM), or Vehicle for 40 min. Student’s t test was used for analysis. * P < 0.05 and ** P < 0.01: Vehicle vs. PGA. D-F Concentrations of AEA, 2-AG and PEA in RBL-2H3 cells treated with PGA (10 µM), or Vehicle for 40 min. Student’s t test was used for analysis. * P < 0.05: Vehicle vs. PGA.

Article Snippet: Deuterated standards—[ 2 H] 8 -AEA, [ 2 H] 5 −2-AG and [ 2 H] 4 -PEA—were purchased from Cayman Chemical (Cabru, Arcore, Italy).

Techniques:

Journal: Scientific Reports

Article Title: Deciphering the interaction between N -palmitoyl-D-glucosamine and the endocannabinoidome

doi: 10.1038/s41598-025-07103-5

Figure Lengend Snippet: Effect of Glucosamine on the endogenous levels of AEA, 2-AG and PEA in HaCaT cells. A-C Concentrations of AEA, 2-AG and PEA in HaCaT cells treated with Glucosamine (10 µM), or Vehicle for 40 min. D-F Concentrations of AEA, 2-AG and PEA in HaCaT cells treated with Glucosamine (10 µM), or Vehicle for 6 h. Student’s t test was used for analysis.

Article Snippet: Deuterated standards—[ 2 H] 8 -AEA, [ 2 H] 5 −2-AG and [ 2 H] 4 -PEA—were purchased from Cayman Chemical (Cabru, Arcore, Italy).

Techniques:

Journal: Scientific Reports

Article Title: Deciphering the interaction between N -palmitoyl-D-glucosamine and the endocannabinoidome

doi: 10.1038/s41598-025-07103-5

Figure Lengend Snippet: Effect of MAFP (10 µM) on the endogenous levels of AEA, 2-AG and PEA in PGA-treated HaCaT cells. A-C Concentrations of AEA, 2-AG and PEA in HaCaT cells co-treated with PGA (10 µM) and MAFP (10 µM), or Vehicle for 40 min. One-way ANOVA followed by Tukey’s multiple comparison test was used for analysis. ** P < 0.01 and **** P < 0.0001: Vehicle vs. PGA; ** P < 0.01 and **** P < 0.0001: Vehicle mix vs. PGA + MAFP.

Article Snippet: Deuterated standards—[ 2 H] 8 -AEA, [ 2 H] 5 −2-AG and [ 2 H] 4 -PEA—were purchased from Cayman Chemical (Cabru, Arcore, Italy).

Techniques: Comparison

Journal: Scientific Reports

Article Title: Deciphering the interaction between N -palmitoyl-D-glucosamine and the endocannabinoidome

doi: 10.1038/s41598-025-07103-5

Figure Lengend Snippet: Effect of PGA on the endogenous levels of AEA, 2-AG and PEA in ABHD4-silenced HaCaT cells. A Bar chart with individual points showing the mRNA expression levels of ABHD4 in control HaCaT cells (scramble) and siRNA ABHD4 transfected HaCaT cells. Student’s t test was used for analysis. **** P < 0.0001: scramble vs. siRNA. B-D Concentrations of AEA, 2-AG and PEA in ABHD4-silenced HaCaT cells treated with PGA (10 µM), or Vehicle for 40 min. Student’s t test was used for analysis. * P < 0.05 and *** P < 0.001: Vehicle vs. PGA.

Article Snippet: Deuterated standards—[ 2 H] 8 -AEA, [ 2 H] 5 −2-AG and [ 2 H] 4 -PEA—were purchased from Cayman Chemical (Cabru, Arcore, Italy).

Techniques: Expressing, Control, Transfection