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Becton Dickinson advantage genomic pcr kit
Advantage Genomic Pcr Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/advantage genomic pcr kit/product/Becton Dickinson
Average 88 stars, based on 2 article reviews
Price from $9.99 to $1999.99
advantage genomic pcr kit - by Bioz Stars, 2020-05
88/100 stars

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Polymerase Chain Reaction:

Article Title: Analysis of tomato gene promoters activated in syncytia induced in tomato and potato hairy roots by Globodera rostochiensis
Article Snippet: .. The 5′ upstream regions of these genes were amplified using a BD Advantage™ Genomic PCR kit (BD Biosciences Clontech) from adaptor-ligated tomato genomic libraries prepared by the GenomeWalker™ protocol (BD Bioscences Clontech). .. Genomic DNA was isolated from frozen tomato leaves using the CTAB method.

Article Title: Cell-Specific Expression of Homospermidine Synthase, the Entry Enzyme of the Pyrrolizidine Alkaloid Pathway in Senecio vernalis, in Comparison with Its Ancestor, Deoxyhypusine Synthase 1
Article Snippet: .. The 2.0-kb fragment of the genomic DNA directly upstream of the open reading frame was amplified by PCR using the Advantage Genomic PCR Kit (BD Biosciences Clontech, Palo Alto, CA) and two specific primers, with the forward primer including a native Hin dIII restriction site 1,871 pb upstream of the start-ATG of the DHS gene and the reverse primer encompassing a Spe I site directly behind the start-ATG of the open reading frame. .. The Hin dIII-/ Spe I-digested PCR product was inserted into pCAMBIA1304, replacing the cauliflower mosaic virus 35S promoter directly in front of an mGFP5*/gusA fusion.

Amplification:

Article Title: Analysis of tomato gene promoters activated in syncytia induced in tomato and potato hairy roots by Globodera rostochiensis
Article Snippet: .. The 5′ upstream regions of these genes were amplified using a BD Advantage™ Genomic PCR kit (BD Biosciences Clontech) from adaptor-ligated tomato genomic libraries prepared by the GenomeWalker™ protocol (BD Bioscences Clontech). .. Genomic DNA was isolated from frozen tomato leaves using the CTAB method.

Article Title: Cell-Specific Expression of Homospermidine Synthase, the Entry Enzyme of the Pyrrolizidine Alkaloid Pathway in Senecio vernalis, in Comparison with Its Ancestor, Deoxyhypusine Synthase 1
Article Snippet: .. The 2.0-kb fragment of the genomic DNA directly upstream of the open reading frame was amplified by PCR using the Advantage Genomic PCR Kit (BD Biosciences Clontech, Palo Alto, CA) and two specific primers, with the forward primer including a native Hin dIII restriction site 1,871 pb upstream of the start-ATG of the DHS gene and the reverse primer encompassing a Spe I site directly behind the start-ATG of the open reading frame. .. The Hin dIII-/ Spe I-digested PCR product was inserted into pCAMBIA1304, replacing the cauliflower mosaic virus 35S promoter directly in front of an mGFP5*/gusA fusion.

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    Becton Dickinson advantage rt for pcr kit
    Expression levels of CXCL10 and IFN-γ genes in skin biopsy specimens from leprosy patients. (A to H) Real-time quantitative <t>RT-PCR</t> was performed on <t>cDNA</t> obtained from sequential skin biopsy specimens from individual leprosy patients who were placed
    Advantage Rt For Pcr Kit, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/advantage rt for pcr kit/product/Becton Dickinson
    Average 92 stars, based on 46 article reviews
    Price from $9.99 to $1999.99
    advantage rt for pcr kit - by Bioz Stars, 2020-05
    92/100 stars
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    Expression levels of CXCL10 and IFN-γ genes in skin biopsy specimens from leprosy patients. (A to H) Real-time quantitative RT-PCR was performed on cDNA obtained from sequential skin biopsy specimens from individual leprosy patients who were placed

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Increased CXC Ligand 10 Levels and Gene Expression in Type 1 Leprosy Reactions ▿

    doi: 10.1128/CVI.00042-11

    Figure Lengend Snippet: Expression levels of CXCL10 and IFN-γ genes in skin biopsy specimens from leprosy patients. (A to H) Real-time quantitative RT-PCR was performed on cDNA obtained from sequential skin biopsy specimens from individual leprosy patients who were placed

    Article Snippet: RNA was converted to cDNA using the Advantage cDNA Polymerase Mix and Advantage RT-for-PCR Kit (BD Bio-Sciences, San Jose, CA) according to the manufacturer's recommendations with oligo(dT) primer.

    Techniques: Expressing, Quantitative RT-PCR

    HIF-1α mRNA transcription under the impact of CoCl 2 or GSNO. (A) HEK293 cells were stimulated with 1 mM GSNO or 100 μM CoCl 2 for 2 h. HIF-1α mRNA was quantified by reverse transcription and PCR (RT-PCR). DNA products were visualized with ethidium bromide on 2% agarose gels. (B) Cells were preincubated for 30 min with the indicated concentrations of actinomycin D followed by incubations with 1 mM GSNO for 4 h. Western analysis with an anti–HIF-1α mAb was used to detect HIF-1α protein. Each experiment was performed at least three times and representative data are shown.

    Journal: Molecular Biology of the Cell

    Article Title: Nitric Oxide Impairs Normoxic Degradation of HIF-1? by Inhibition of Prolyl Hydroxylases

    doi: 10.1091/mbc.E02-12-0791

    Figure Lengend Snippet: HIF-1α mRNA transcription under the impact of CoCl 2 or GSNO. (A) HEK293 cells were stimulated with 1 mM GSNO or 100 μM CoCl 2 for 2 h. HIF-1α mRNA was quantified by reverse transcription and PCR (RT-PCR). DNA products were visualized with ethidium bromide on 2% agarose gels. (B) Cells were preincubated for 30 min with the indicated concentrations of actinomycin D followed by incubations with 1 mM GSNO for 4 h. Western analysis with an anti–HIF-1α mAb was used to detect HIF-1α protein. Each experiment was performed at least three times and representative data are shown.

    Article Snippet: HIF-1α antibodies, Advantage RT-for-PCR kit, AdvanTaq PCR kit were purchased from Becton Dickinson (Heidelberg, Germany).

    Techniques: Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Western Blot

    RT-PCR analysis of MTMR13 expression in several human tissues. A, RT-PCR performed using primers amplifying a fragment corresponding to the first 1,250 bp of the MTMR13 ORF, starting from the ATG. A similar expression pattern was demonstrated for the remaining four overlapping fragments into which the ORF was divided (not shown). B, G3PDH was used as positive control for the quality of RNA and cDNA synthesis. −MMLV is a control reaction in which the MMLV reverse transcriptase was omitted. RT corresponds to the negative control of the PCR performed by omitting the cDNA thereafter.

    Journal: American Journal of Human Genetics

    Article Title: Mutations in MTMR13, a New Pseudophosphatase Homologue of MTMR2 and Sbf1, in Two Families with an Autosomal Recessive Demyelinating Form of Charcot-Marie-Tooth Disease Associated with Early-Onset Glaucoma

    doi:

    Figure Lengend Snippet: RT-PCR analysis of MTMR13 expression in several human tissues. A, RT-PCR performed using primers amplifying a fragment corresponding to the first 1,250 bp of the MTMR13 ORF, starting from the ATG. A similar expression pattern was demonstrated for the remaining four overlapping fragments into which the ORF was divided (not shown). B, G3PDH was used as positive control for the quality of RNA and cDNA synthesis. −MMLV is a control reaction in which the MMLV reverse transcriptase was omitted. RT corresponds to the negative control of the PCR performed by omitting the cDNA thereafter.

    Article Snippet: First-strand cDNA was prepared from 1 μg of RNA, through use of the Advantage RT-for-PCR kit (Becton Dickinson; Clontech).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Positive Control, Negative Control, Polymerase Chain Reaction

    Predominant expression in tumor cells of CML66-L but not CML66-S. A , The tissue expression of CML66-S in tumor cell lines vs testis was examined by semiquantitative RT-PCR revealed by 1% agarose gel followed by Southern blot hybridization (not shown). In addition to RNA samples from cells, no RNA control was used as a negative control in RT-PCR. CML66-S-specific transcripts were amplified in RT-PCR with 35 cycles to detect CML66-S transcripts at very low levels in tumor cell lines. As controls, CML66-L-specific transcripts and GAPDH transcripts were also examined in RT-PCR with 30 cycles. The lower panels show the ratios of CML66-S signal over GAPDH signal (internal housekeeping gene control) as the normalized quantitation (relative densitometric units) of CML66-S expression in each sample, respectively. The expression of CML66-S was mostly found in testis but was expressed in very low levels in tumor cell lines even after 35 cycles of PCR amplification. In contrast, the CML66-L transcript was detected in four tumor cell lines and human testis with 30 cycles of PCR amplification. B , The expression of CML66-L in tumor samples from patients. The hybridization analyses of the matched tumor/normal expression array (BD Clontech) with 32 P-labeled CML66-L-specific probe, CML66-S-specific probe, and ubiquitin probe were performed, respectively. The densitometric ratios of CML66-L expression in the matched tumor samples vs that in matched normal tissues were calculated. The statistical mean ( X ̄ ) plus 3 SD (1.135) of CML66-L expression signals among normal samples is shown as the variation range of CML66 signal among the normal samples. CML66-L expression signals in tumors higher than this upper limit of normal range are considered positive.

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: A Novel Mechanism of Alternative Promoter and Splicing Regulates the Epitope Generation of Tumor Antigen CML66-L

    doi:

    Figure Lengend Snippet: Predominant expression in tumor cells of CML66-L but not CML66-S. A , The tissue expression of CML66-S in tumor cell lines vs testis was examined by semiquantitative RT-PCR revealed by 1% agarose gel followed by Southern blot hybridization (not shown). In addition to RNA samples from cells, no RNA control was used as a negative control in RT-PCR. CML66-S-specific transcripts were amplified in RT-PCR with 35 cycles to detect CML66-S transcripts at very low levels in tumor cell lines. As controls, CML66-L-specific transcripts and GAPDH transcripts were also examined in RT-PCR with 30 cycles. The lower panels show the ratios of CML66-S signal over GAPDH signal (internal housekeeping gene control) as the normalized quantitation (relative densitometric units) of CML66-S expression in each sample, respectively. The expression of CML66-S was mostly found in testis but was expressed in very low levels in tumor cell lines even after 35 cycles of PCR amplification. In contrast, the CML66-L transcript was detected in four tumor cell lines and human testis with 30 cycles of PCR amplification. B , The expression of CML66-L in tumor samples from patients. The hybridization analyses of the matched tumor/normal expression array (BD Clontech) with 32 P-labeled CML66-L-specific probe, CML66-S-specific probe, and ubiquitin probe were performed, respectively. The densitometric ratios of CML66-L expression in the matched tumor samples vs that in matched normal tissues were calculated. The statistical mean ( X ̄ ) plus 3 SD (1.135) of CML66-L expression signals among normal samples is shown as the variation range of CML66 signal among the normal samples. CML66-L expression signals in tumors higher than this upper limit of normal range are considered positive.

    Article Snippet: A cDNA fragment encoding the 38 aa in CML66-L-specific N terminus was generated by PCR using the Advantage HF (high-fidelity) PCR kit (BD Clontech), sense primer 385 (5′- CCGGGAATTCA GAGGTGGCGGCTAATTGCTCCCTA-3′), and antisense primer 383 (5′- CCGGGAATTCCCGGG TGCGTCAAGCTCCAGCTGGTAACA-3′).

    Techniques: Expressing, Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis, Southern Blot, Hybridization, Negative Control, Amplification, Quantitation Assay, Polymerase Chain Reaction, Labeling