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Advanced Analytical Inc advanced analytical fragment analyzer
Advanced Analytical Fragment Analyzer, supplied by Advanced Analytical Inc, used in various techniques. Bioz Stars score: 89/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/advanced analytical fragment analyzer/product/Advanced Analytical Inc
Average 89 stars, based on 5 article reviews
Price from $9.99 to $1999.99
advanced analytical fragment analyzer - by Bioz Stars, 2020-09
89/100 stars

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Real-time Polymerase Chain Reaction:

Article Title: Transcriptomic analysis reveals specific metabolic pathways of enterohemorrhagic Escherichia coli O157:H7 in bovine digestive contents
Article Snippet: .. Library quality was assessed using an Advanced Analytical Fragment Analyzer and libraries were quantified by qPCR using the Kapa Library Quantification kit. .. RNA-seq experiments were performed on an Illumina HiSeq3000 using a paired-end read length of 2x150 bp with the Illumina HiSeq3000 chemistry.

Electrophoresis:

Article Title: Miniaturization and optimization of 384-well compatible RNA sequencing library preparation
Article Snippet: .. To check the quality of libraries prepared using the miniaturized protocol, we used a parallel capillary electrophoresis assay to process up to 95 samples simultaneously (Advanced Analytical Fragment Analyzer). .. Libraries were assessed for distribution of cDNA fragment size, estimated molarity and concentration, and for the presence of primer- and adaptor-dimers.

Quantitation Assay:

Article Title: Whole blood transcriptome analysis reveals footprints of cattle adaptation to sub‐arctic conditions
Article Snippet: .. The high quality of the libraries was confirmed with an Advanced Analytical Fragment Analyzer, and the concentrations of the libraries were quantified using Qubit® Fluorometric Quantitation (Life Technologies). .. The average RNA‐seq library fragments were in the range of 250–350 bp.

other:

Article Title: Poly(A)-specific ribonuclease (PARN) mediates 3′-end maturation of the telomerase RNA component
Article Snippet: The quality and quantity of each library was determined on an Advanced Analytical Fragment Analyzer.

Article Title: Effective CRISPR interference of an endogenous gene via a single transgene in mice
Article Snippet: The quality and quantity of input RNA was determined by use of the Advanced Analytical Fragment Analyzer (AATI) and Qubit (Life Technologies) instruments respectively.

Article Title: Up in Arms: Immune and Nervous System Response to Sea Star Wasting Disease
Article Snippet: RNA quality was assessed using an Advanced Analytical Fragment Analyzer.

Sequencing:

Article Title: Genome-resolved metagenomics of eukaryotic populations during early colonization of premature infants and in hospital rooms
Article Snippet: .. Final sequence ready libraries were visualized and quantified on the Advanced Analytical Fragment Analyzer, pooled into 11 subpools based on mass, and checked for pooling accuracy by sequencing on Illumina MiSeq Nano sequencing runs. .. Libraries were then further purified using 1.5% Pippin Prep gel size selection assays collecting library pools from 500 to 700 bp.

Molecular Weight:

Article Title: Pharmacologic Reprogramming Designed to Induce a Warburg Effect in Porcine Fetal Fibroblasts Alters Gene Expression and Quantities of Metabolites from Conditioned Media Without Increased Cell Proliferation
Article Snippet: .. Total RNA quality was assessed at the University of Missouri DNA core facility by using the Advanced Analytical Fragment Analyzer and RNA quality scores were assigned based on (1) the presence of discrete 18S and 28S rRNA bands, (2) the mass ratio between the 28S and 18S rRNA, (3) the absence of fragments in the pre-18S and 28S regions, and (4) absence of contaminating high molecular weight fragments. ..

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    Advanced Analytical Inc fragment analyzer total rna
    EndoU-deficient <t>MHV</t> induces activation of the OAS-RNase L pathway, resulting in early breakdown of ribosomal <t>RNA.</t> ( a ) Analysis of rRNA integrity in bone marrow-derived macrophages derived from wild type C57BL/6, Mda5 -/- , RNase L -/- , and IFNAR -/- mice following infection with MHV-A59 and MHV H277A (MOI = 1). Total RNA was isolated at indicated time points and degradation of ribosomal RNA as marker for RNase L activation was assessed with a Fragment Analyzer. One representative picture and migration of 18S and 28S ribosomal RNA is displayed. The RNA Quality Number (RQN) is indicated. ( b ) The integrity of rRNA from MHV-A59 and MHV H277A infected (MOI = 1) L929 cells, with or without IFN-I pre-treatment (12.5 U of IFN-I 16h prior to infection). Analysis was performed as in panel ( a ) and one representative image out of five is displayed.
    Fragment Analyzer Total Rna, supplied by Advanced Analytical Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fragment analyzer total rna/product/Advanced Analytical Inc
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fragment analyzer total rna - by Bioz Stars, 2020-09
    91/100 stars
      Buy from Supplier

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    EndoU-deficient MHV induces activation of the OAS-RNase L pathway, resulting in early breakdown of ribosomal RNA. ( a ) Analysis of rRNA integrity in bone marrow-derived macrophages derived from wild type C57BL/6, Mda5 -/- , RNase L -/- , and IFNAR -/- mice following infection with MHV-A59 and MHV H277A (MOI = 1). Total RNA was isolated at indicated time points and degradation of ribosomal RNA as marker for RNase L activation was assessed with a Fragment Analyzer. One representative picture and migration of 18S and 28S ribosomal RNA is displayed. The RNA Quality Number (RQN) is indicated. ( b ) The integrity of rRNA from MHV-A59 and MHV H277A infected (MOI = 1) L929 cells, with or without IFN-I pre-treatment (12.5 U of IFN-I 16h prior to infection). Analysis was performed as in panel ( a ) and one representative image out of five is displayed.

    Journal: PLoS Pathogens

    Article Title: Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication

    doi: 10.1371/journal.ppat.1006195

    Figure Lengend Snippet: EndoU-deficient MHV induces activation of the OAS-RNase L pathway, resulting in early breakdown of ribosomal RNA. ( a ) Analysis of rRNA integrity in bone marrow-derived macrophages derived from wild type C57BL/6, Mda5 -/- , RNase L -/- , and IFNAR -/- mice following infection with MHV-A59 and MHV H277A (MOI = 1). Total RNA was isolated at indicated time points and degradation of ribosomal RNA as marker for RNase L activation was assessed with a Fragment Analyzer. One representative picture and migration of 18S and 28S ribosomal RNA is displayed. The RNA Quality Number (RQN) is indicated. ( b ) The integrity of rRNA from MHV-A59 and MHV H277A infected (MOI = 1) L929 cells, with or without IFN-I pre-treatment (12.5 U of IFN-I 16h prior to infection). Analysis was performed as in panel ( a ) and one representative image out of five is displayed.

    Article Snippet: Measurement of RNA quality with Fragment Analyzer Total RNA isolated from MHV-infected macrophages and L929 cells was analysed with a Fragment Analyzer (Labgene) using the DNF-471 standard sensitivity RNA analysis kit (15nt lower marker, Advanced Analytical Technologies).

    Techniques: Activation Assay, Derivative Assay, Mouse Assay, Infection, Isolation, Marker, Migration

    Replication of EndoU-deficient MHV is partially restored in IFNAR -/- macrophages and EndoU mutants display a pronounced sensitivity to IFN-I treatment. ( a ) Replication kinetics of MHV-A59 and MHV H277A (left panel; titers in pfu) and cell-associated viral RNA (right panel; qRT-PCR) following infection of IFNAR -/- bone marrow-derived macrophages (MOI = 1). Data represent four independent experiments, each performed in two to three replicas. Mean and SEM are depicted. The 95% confidence band is highlighted in grey. The differences in peak levels of viral titers (MHV-A59: 6.0, MHV H277A : 5.2) and RNA copies (MHV-A59: 9.7, MHV H277A : 9.3) were statistically significant (p

    Journal: PLoS Pathogens

    Article Title: Early endonuclease-mediated evasion of RNA sensing ensures efficient coronavirus replication

    doi: 10.1371/journal.ppat.1006195

    Figure Lengend Snippet: Replication of EndoU-deficient MHV is partially restored in IFNAR -/- macrophages and EndoU mutants display a pronounced sensitivity to IFN-I treatment. ( a ) Replication kinetics of MHV-A59 and MHV H277A (left panel; titers in pfu) and cell-associated viral RNA (right panel; qRT-PCR) following infection of IFNAR -/- bone marrow-derived macrophages (MOI = 1). Data represent four independent experiments, each performed in two to three replicas. Mean and SEM are depicted. The 95% confidence band is highlighted in grey. The differences in peak levels of viral titers (MHV-A59: 6.0, MHV H277A : 5.2) and RNA copies (MHV-A59: 9.7, MHV H277A : 9.3) were statistically significant (p

    Article Snippet: Measurement of RNA quality with Fragment Analyzer Total RNA isolated from MHV-infected macrophages and L929 cells was analysed with a Fragment Analyzer (Labgene) using the DNF-471 standard sensitivity RNA analysis kit (15nt lower marker, Advanced Analytical Technologies).

    Techniques: Quantitative RT-PCR, Infection, Derivative Assay