Journal: Frontiers in Behavioral Neuroscience

Article Title: Strain-dependent regulation of hippocampal long-term potentiation by dopamine D1/D5 receptors in mice

doi: 10.3389/fnbeh.2022.1023361

Figure Lengend Snippet: Expression of D1 and D5 receptors are different in CaOlaHsd and C57Bl/6 mice. (A,B) The graphs depict the optical density of D1R (A) and D5R (B) expression in different layers of CA1 in CaOlaHsd (open black circles) and C57Bl/6 (closed gray circles) mice. D1 receptor expression is significantly higher in CaOlaHsd mice compared to C57Bl/6 mice in SLM (A) . The CA1 sublayers assessed show no differences with regard to D5 receptor expression in CaOlaHsd mice compared to C57Bl/6 mice (B) . SLM: stratum lacunosum moleculare, SO: stratum oriens, SP: stratum pyramidale, SR: stratum radiatum. (C,D) Immunostainings of the different layers in the CA1 region of CaOlaHsd and C57Bl/6 mice. The dotted magenta lines in (C) outline the stratum lacunosum moleculare, where D1R (C) showed increased expression in CaOlaHsd mice. No differences in expression of D5R were detected between strains (D) . Scale bar = 200 μm. Significant values are denoted by an asterisk. * p < 0.05.

Article Snippet: Subsequently, the sections were incubated overnight at room temperature with the primary antibody solution, which contained 20% biotin (avidin-biotin blocking kit, Vector Laboratories, Newark, CA, USA), 1% blocking serum, 0.2% Tx, and the primary antibody with these concentrations: D1R (1:100, rabbit polyclonal, ADR-005, Alomone Labs, Jerusalem, Israel), D5R (1:500, rabbit polyclonal, AB1765P, Sigma-Aldrich, Darmstadt, Germany).

Techniques: Expressing

Journal: Fluids and Barriers of the CNS

Article Title: Modulation of striatal glutamatergic, dopaminergic and cholinergic neurotransmission pathways concomitant with motor disturbance in rats with kaolin-induced hydrocephalus

doi: 10.1186/s12987-022-00393-1

Figure Lengend Snippet: Changes in the expressions of the striatal dopaminergic and cholinergic neurotransmitter markers in hydrocephalic rats. A shows representative Western blots of the expression of TH in the striatum of hydrocephalic and age-matched control ( n = 4–12 rats per group). B Representative TH immunolabeling from the dorsolateral striatum of the acute and chronic hydrocephalic ( b1 and b2 , respectively) and corresponding age-matched controls ( b3 and b4 ) ( n = 3 rats for each group). C – E are representative Western blots and corresponding analyses for the expressions of striatal DR1 and 2 and ChAT ( n = 4–11 rats per group). The expression of the GAPDH is the internal gel-loading control. The expression of each protein was normalized to that GAPDH and presented as a ratio to that of the age-matched sham-operated control animals. Con, control; Hydro, hydrocephalus; TH, tyrosine hydroxylase; DR1 and DR2, type 1 and type 2 dopamine receptors; ChAT, choline acetyltransferase; GAPDH, glyceraldehyde-3-phosphate dehydrogenase. * P < 0.05 and ** P < 0.001 between the marked and the corresponding age-matched control. Scale bar = 100 μm

Article Snippet: Primary antibodies employed included mouse anti-synaptophysin (MAB5258; Millipore Billerica, MA, USA), mouse anti-VGLUT1 (MAB5502; Millipore), mouse anti-PSD95 (MAB1598; Millipore), mouse anti-NR1 (32-0500; Thermo-Fisher Scientific, Pittsburgh, PA, USA), rabbit anti-GluR1 (PA1-37776; Thermo-Fisher Scientific), rabbit anti-GluR2/3 (PA1-4660; Thermo-Fisher Scientific), rabbit anti-TH (ab117112; Abcam), rabbit anti-DR1 (ab20066; Abcam), rabbit anti-DR2 (ADR-002; Alomone Labs, Jerusalem, Israel), goat anti-ChAT (AB144P; Millipore) and rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (sc-25778; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Western Blot, Expressing, Immunolabeling

Journal: Fluids and Barriers of the CNS

Article Title: Modulation of striatal glutamatergic, dopaminergic and cholinergic neurotransmission pathways concomitant with motor disturbance in rats with kaolin-induced hydrocephalus

doi: 10.1186/s12987-022-00393-1

Figure Lengend Snippet: Radar plots summarizing the effects of hydrocephalus on the striatal marker proteins that were investigated. Two-dimensional radar plots was used to depict the expressions of multiple proteins in acute and chronic hydrocephalus in reference to the expressions of their age-matched sham-operated control counterparts. Each axis shows the mean of the hydrocephalic (filled circle) and the corresponding control (empty circle) with 0 expression at the center and full expression out to the periphery. For each data point, the expression of the named marker protein was first standardized to that of the corresponding internal gel-loading control and then to the mean of the age-matched sham-operated group. The area enclosed by the lines connecting between the hydrocephalic or control data points of the adjacent axes represents the relative magnitudes of the expressions of the proteins of the hydrocephalic and age-matched sham-operated control rats respectively. The area enclosed by the data points of hydrocephalic group was shaded darker for easier visualization. Glutamatergic-related protein expressions are grouped and plotted in A and B , while dopaminergic and cholinergic modulatory action-related are shown in C and D . The expressions under acute and chronic hydrocephalus were plotted separately as indicated. Hydrocephalus affected glutamatergic connections instantly at the acute stage and persisted to the chronic phase. The effect on DR2 expression was however delayed and showed up during chronic hydrocephalus. VGLUT1, vesicular glutamate transporter 1; PSD95, postsynaptic density protein 95; GluR2/3, AMPA glutamate receptor subunit 2/3; DR1 and DR2, type 1 and type 2 dopamine receptors; ChAT, choline acetyltransferase

Article Snippet: Primary antibodies employed included mouse anti-synaptophysin (MAB5258; Millipore Billerica, MA, USA), mouse anti-VGLUT1 (MAB5502; Millipore), mouse anti-PSD95 (MAB1598; Millipore), mouse anti-NR1 (32-0500; Thermo-Fisher Scientific, Pittsburgh, PA, USA), rabbit anti-GluR1 (PA1-37776; Thermo-Fisher Scientific), rabbit anti-GluR2/3 (PA1-4660; Thermo-Fisher Scientific), rabbit anti-TH (ab117112; Abcam), rabbit anti-DR1 (ab20066; Abcam), rabbit anti-DR2 (ADR-002; Alomone Labs, Jerusalem, Israel), goat anti-ChAT (AB144P; Millipore) and rabbit anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (sc-25778; Santa Cruz Biotechnology, Santa Cruz, CA, USA) antibodies.

Techniques: Marker, Expressing

Journal: International Journal of Molecular Sciences

Article Title: Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review

doi: 10.3390/ijms222312932

Figure Lengend Snippet: Animal studies included in the analysis. Abbreviations: 6-OHDA: 2,4,5-trihydroxyphenethylamine; DAI: disease activity index; DNBF: 2,4-dinitrofluorobenzene; DSS: dextran sulphate sodium; IA: iodoacetamide; MPO: mieloperoxidase; MPTP: 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine; TNBS: 2,4,6-trinitrobenzene sulfonic acid; routes of administration: i.p.: intraperitoneal; i.g.: intragastric; i.v.: intravenous; s.c.: subcutaneous.

Article Snippet: Ugalde et al., 2021 [ ] , Hematoxylin and eosin, Alcian blue, Zombie Aqua (Biolegend, San Diego, CA, USA), Cell trace violet (Invitrogen, USA) , Anti-FoxP3 (clone FJK-16S), -IFNγ (clone XMG1.2), -α4β7 (clone DATK32), -CCR9 (clone CW.1.2) (eBioscience, USA); anti-CD4 (clone GK1.5),-CD25 (clone PC61), -CD44 (clone IM7), -CD62L (clone MEL14), -IL-17A (clone TC11-181710.1), -CD45.2 (clone 104), -CD45.1 (clone A20), -TCRVα2 (clone B20.1), -TCRVβ5 (clone MR9-4), -CD28 (clone 37.51),-CD3ε (clone 145-2C11), -IFNγ (clone AN-18) (Biolegend, USA); anti-DRD3 (ADR-003) (Alomone labs).

Techniques: Activity Assay, Generated, Ex Vivo, shRNA, Concentration Assay, Knock-Out, Phagocytosis Assay

Journal: International Journal of Molecular Sciences

Article Title: Gastrointestinal Dopamine in Inflammatory Bowel Diseases: A Systematic Review

doi: 10.3390/ijms222312932

Figure Lengend Snippet: Dyes and antibodies used for in vivo and in vitro histological analysis and/or immunostaining (immunohistochemistry, immunoblotting, flow cytometry, enzyme-linked immunosorbent assay/ELISA).

Article Snippet: Ugalde et al., 2021 [ ] , Hematoxylin and eosin, Alcian blue, Zombie Aqua (Biolegend, San Diego, CA, USA), Cell trace violet (Invitrogen, USA) , Anti-FoxP3 (clone FJK-16S), -IFNγ (clone XMG1.2), -α4β7 (clone DATK32), -CCR9 (clone CW.1.2) (eBioscience, USA); anti-CD4 (clone GK1.5),-CD25 (clone PC61), -CD44 (clone IM7), -CD62L (clone MEL14), -IL-17A (clone TC11-181710.1), -CD45.2 (clone 104), -CD45.1 (clone A20), -TCRVα2 (clone B20.1), -TCRVβ5 (clone MR9-4), -CD28 (clone 37.51),-CD3ε (clone 145-2C11), -IFNγ (clone AN-18) (Biolegend, USA); anti-DRD3 (ADR-003) (Alomone labs).

Techniques: In Vivo, In Vitro, Immunostaining, Immunohistochemistry, Western Blot, Flow Cytometry, Enzyme-linked Immunosorbent Assay