adenosine tri phosphate atp  (Millipore)

 
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  • 99
    Name:
    Adenosine 5 triphosphate ATP disodium salt hydrate
    Description:

    Catalog Number:
    FLAAS
    Price:
    None
    Applications:
    Adenosine 5′-triphosphate (ATP) disodium salt hydrate has been:. used as a standard to measure ATP by bioluminescence method. used in kinase buffer to assess the interaction between death-associated protein kinases (DAPK2) and 14-3-3-β. used as a standard in ATP colorimetric assay
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    Structured Review

    Millipore adenosine tri phosphate atp
    Adenosine 5 triphosphate ATP disodium salt hydrate

    https://www.bioz.com/result/adenosine tri phosphate atp/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    adenosine tri phosphate atp - by Bioz Stars, 2021-09
    99/100 stars

    Images

    1) Product Images from "Mitochondrial respiratory chain adaptations in macrophages contribute to antibacterial host defence"

    Article Title: Mitochondrial respiratory chain adaptations in macrophages contribute to antibacterial host defence

    Journal: Nature immunology

    doi: 10.1038/ni.3509

    Detection of bacteria induces changes in mitochondrial ETC complex activities and influences mitochondrial respiration and glycolysis. ( a ) Spectrophotometric activities of the indicated mitochondrial respiratory complexes, normalized to citrate synthase (CS) activity in mitochondria isolated from BMDMs treated or not with E. coli for 1.5h. Specific activities (in IU/mg protein) corresponding to 100% activity are 0.105±0.013, 0.040±0.008, 0.055±0.01, 0.1±0.03, 0.23±0.01, 0.02±0.003, 0.3±0.01 for CI, CII, CIII, CIV, CI+III, CII+III and CS, respectively. ( b ) Effect of EC-stimulation on WT BMDMs glutamate+malate-driven ATP synthesis. 100% activity corresponds to a rate of 153±24.9 nmol ATP/min/mg protein. ( c-f ) Oxygen consumption rate (OCR) upon sequential treatment of oligomycin (olig.), CCCP, and rotenone+antimycin (Rot.+Ant.) ( c ) spare respiratory capacity (SRC) ( d ), basal extracellular acidification rate (ECAR) ( e ) and extracellular lactate concentration ( f ) in WT BMDMs treated or not with EC for 2h. NS, not significant; *P
    Figure Legend Snippet: Detection of bacteria induces changes in mitochondrial ETC complex activities and influences mitochondrial respiration and glycolysis. ( a ) Spectrophotometric activities of the indicated mitochondrial respiratory complexes, normalized to citrate synthase (CS) activity in mitochondria isolated from BMDMs treated or not with E. coli for 1.5h. Specific activities (in IU/mg protein) corresponding to 100% activity are 0.105±0.013, 0.040±0.008, 0.055±0.01, 0.1±0.03, 0.23±0.01, 0.02±0.003, 0.3±0.01 for CI, CII, CIII, CIV, CI+III, CII+III and CS, respectively. ( b ) Effect of EC-stimulation on WT BMDMs glutamate+malate-driven ATP synthesis. 100% activity corresponds to a rate of 153±24.9 nmol ATP/min/mg protein. ( c-f ) Oxygen consumption rate (OCR) upon sequential treatment of oligomycin (olig.), CCCP, and rotenone+antimycin (Rot.+Ant.) ( c ) spare respiratory capacity (SRC) ( d ), basal extracellular acidification rate (ECAR) ( e ) and extracellular lactate concentration ( f ) in WT BMDMs treated or not with EC for 2h. NS, not significant; *P

    Techniques Used: Activity Assay, Isolation, Concentration Assay

    2) Product Images from "A Metabotropic-Like Flux-Independent NMDA Receptor Regulates Ca2+ Exit from Endoplasmic Reticulum and Mitochondrial Membrane Potential in Cultured Astrocytes"

    Article Title: A Metabotropic-Like Flux-Independent NMDA Receptor Regulates Ca2+ Exit from Endoplasmic Reticulum and Mitochondrial Membrane Potential in Cultured Astrocytes

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0126314

    Genistein effect on i Ca 2+ response to NMDA and ATP response. ( A ) ΔF/F 0 averaged response in Fluo-4-AM-labeled rCCA perfused with 1 mM NMDA in the presence of genistein (GX). ( B ) ΔF/F 0 averaged response in Fluo-4-AM-labeled rCCA perfused with ATP. Line above traces indicates perfusion with 1 mM NMDA after 120 sec basal recording. Line below (if applicable) indicates the inhibitor used throughout the recording time. ( C ) ∫(ΔF/F 0 ) distribution histogram for cell population responses in genistein and ATP conditions; the NMDA treated population distribution is included for comparison. The RTV value is indicated by the black arrowhead (see text). Statistical analysis for these distributions with the number of cells and the number of experiments are shown in Table 1 . Representative images from i Ca 2+ responses are shown in S8 Fig .
    Figure Legend Snippet: Genistein effect on i Ca 2+ response to NMDA and ATP response. ( A ) ΔF/F 0 averaged response in Fluo-4-AM-labeled rCCA perfused with 1 mM NMDA in the presence of genistein (GX). ( B ) ΔF/F 0 averaged response in Fluo-4-AM-labeled rCCA perfused with ATP. Line above traces indicates perfusion with 1 mM NMDA after 120 sec basal recording. Line below (if applicable) indicates the inhibitor used throughout the recording time. ( C ) ∫(ΔF/F 0 ) distribution histogram for cell population responses in genistein and ATP conditions; the NMDA treated population distribution is included for comparison. The RTV value is indicated by the black arrowhead (see text). Statistical analysis for these distributions with the number of cells and the number of experiments are shown in Table 1 . Representative images from i Ca 2+ responses are shown in S8 Fig .

    Techniques Used: Labeling, Size-exclusion Chromatography

    Related Articles

    other:

    Article Title: Coronin-1 is phosphorylated at Thr-412 by protein kinase Cα in human phagocytic cells
    Article Snippet: 2.1 Reagents Adenosine 5’-triphosphate (ATP) disodium salt hydrate, 1,4-diazabicyclo-2,2,2-octane, Ficoll PM400, human serum (blood group AB), phosphatidylserine, poly-l-lysine, rhodamine-conjugated phalloidin, Triton X-100 and zymosan A were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Article Title: Antioxidant and Cryoprotective Effects of Bone Hydrolysates from Bighead Carp (Aristichthys nobilis) in Freeze-Thawed Fish Fillets
    Article Snippet: Hippuryl-histidyl-leucine (HHL), bacitracin, aprotinin, cytochrome C, carbonic anhydrase, adenosine triphosphate (ATP), 5,5-dithio-bis (2-nitrobenzoic acid) (DTNB) and 2,4-dinitrophenylhydrazine (DNPH) were obtained from Sigma-Aldrich (Milwaukee, WI, USA).

    Article Title: Topical inflammasome inhibition with disulfiram prevents irritant contact dermatitis, et al. Topical inflammasome inhibition with disulfiram prevents irritant contact dermatitis
    Article Snippet: Upon inflammasome stimulation by activators such as adenosine triphosphate (ATP) or nigericin, ASC assembles into a detectable cytosolic multi‐protein complex named ASC “speck”.

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  • 99
    Millipore adenosine tri phosphate atp
    Detection of bacteria induces changes in mitochondrial ETC complex activities and influences mitochondrial respiration and glycolysis. ( a ) Spectrophotometric activities of the indicated mitochondrial respiratory complexes, normalized to citrate synthase (CS) activity in mitochondria isolated from BMDMs treated or not with E. coli for 1.5h. Specific activities (in IU/mg protein) corresponding to 100% activity are 0.105±0.013, 0.040±0.008, 0.055±0.01, 0.1±0.03, 0.23±0.01, 0.02±0.003, 0.3±0.01 for CI, CII, CIII, CIV, CI+III, CII+III and CS, respectively. ( b ) Effect of EC-stimulation on WT BMDMs glutamate+malate-driven <t>ATP</t> synthesis. 100% activity corresponds to a rate of 153±24.9 nmol ATP/min/mg protein. ( c-f ) Oxygen consumption rate (OCR) upon sequential treatment of oligomycin (olig.), <t>CCCP,</t> and rotenone+antimycin (Rot.+Ant.) ( c ) spare respiratory capacity (SRC) ( d ), basal extracellular acidification rate (ECAR) ( e ) and extracellular lactate concentration ( f ) in WT BMDMs treated or not with EC for 2h. NS, not significant; *P
    Adenosine Tri Phosphate Atp, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adenosine tri phosphate atp/product/Millipore
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    adenosine tri phosphate atp - by Bioz Stars, 2021-09
    99/100 stars
      Buy from Supplier

    97
    Millipore atp regenerating system
    Detection of bacteria induces changes in mitochondrial ETC complex activities and influences mitochondrial respiration and glycolysis. ( a ) Spectrophotometric activities of the indicated mitochondrial respiratory complexes, normalized to citrate synthase (CS) activity in mitochondria isolated from BMDMs treated or not with E. coli for 1.5h. Specific activities (in IU/mg protein) corresponding to 100% activity are 0.105±0.013, 0.040±0.008, 0.055±0.01, 0.1±0.03, 0.23±0.01, 0.02±0.003, 0.3±0.01 for CI, CII, CIII, CIV, CI+III, CII+III and CS, respectively. ( b ) Effect of EC-stimulation on WT BMDMs glutamate+malate-driven <t>ATP</t> synthesis. 100% activity corresponds to a rate of 153±24.9 nmol ATP/min/mg protein. ( c-f ) Oxygen consumption rate (OCR) upon sequential treatment of oligomycin (olig.), <t>CCCP,</t> and rotenone+antimycin (Rot.+Ant.) ( c ) spare respiratory capacity (SRC) ( d ), basal extracellular acidification rate (ECAR) ( e ) and extracellular lactate concentration ( f ) in WT BMDMs treated or not with EC for 2h. NS, not significant; *P
    Atp Regenerating System, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atp regenerating system/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atp regenerating system - by Bioz Stars, 2021-09
    97/100 stars
      Buy from Supplier

    Image Search Results


    Detection of bacteria induces changes in mitochondrial ETC complex activities and influences mitochondrial respiration and glycolysis. ( a ) Spectrophotometric activities of the indicated mitochondrial respiratory complexes, normalized to citrate synthase (CS) activity in mitochondria isolated from BMDMs treated or not with E. coli for 1.5h. Specific activities (in IU/mg protein) corresponding to 100% activity are 0.105±0.013, 0.040±0.008, 0.055±0.01, 0.1±0.03, 0.23±0.01, 0.02±0.003, 0.3±0.01 for CI, CII, CIII, CIV, CI+III, CII+III and CS, respectively. ( b ) Effect of EC-stimulation on WT BMDMs glutamate+malate-driven ATP synthesis. 100% activity corresponds to a rate of 153±24.9 nmol ATP/min/mg protein. ( c-f ) Oxygen consumption rate (OCR) upon sequential treatment of oligomycin (olig.), CCCP, and rotenone+antimycin (Rot.+Ant.) ( c ) spare respiratory capacity (SRC) ( d ), basal extracellular acidification rate (ECAR) ( e ) and extracellular lactate concentration ( f ) in WT BMDMs treated or not with EC for 2h. NS, not significant; *P

    Journal: Nature immunology

    Article Title: Mitochondrial respiratory chain adaptations in macrophages contribute to antibacterial host defence

    doi: 10.1038/ni.3509

    Figure Lengend Snippet: Detection of bacteria induces changes in mitochondrial ETC complex activities and influences mitochondrial respiration and glycolysis. ( a ) Spectrophotometric activities of the indicated mitochondrial respiratory complexes, normalized to citrate synthase (CS) activity in mitochondria isolated from BMDMs treated or not with E. coli for 1.5h. Specific activities (in IU/mg protein) corresponding to 100% activity are 0.105±0.013, 0.040±0.008, 0.055±0.01, 0.1±0.03, 0.23±0.01, 0.02±0.003, 0.3±0.01 for CI, CII, CIII, CIV, CI+III, CII+III and CS, respectively. ( b ) Effect of EC-stimulation on WT BMDMs glutamate+malate-driven ATP synthesis. 100% activity corresponds to a rate of 153±24.9 nmol ATP/min/mg protein. ( c-f ) Oxygen consumption rate (OCR) upon sequential treatment of oligomycin (olig.), CCCP, and rotenone+antimycin (Rot.+Ant.) ( c ) spare respiratory capacity (SRC) ( d ), basal extracellular acidification rate (ECAR) ( e ) and extracellular lactate concentration ( f ) in WT BMDMs treated or not with EC for 2h. NS, not significant; *P

    Article Snippet: 3-nitropropionic acid (NPA), succinate, succinate hexahydrate, glutamate, malate disodium-salt, fumarate, dimethyl-fumarate, dimethyl succinate, dimethyl malonate (DM), itaconic acid, thenoyltrifluoroacetone (TTFA), carbonilcyanide p-triflouromethoxyphenylhydrazone (FCCP), CCCP, oligomycin, rotenone, antimycin A, ubiquinone, sn-glycerol 3-phosphate, oxidized cytochrome c, adenosine tri-phosphate (ATP), adenosine di-phosphate (ADP), phenazine methosulfate (PMS) and digitonin were all from Sigma.

    Techniques: Activity Assay, Isolation, Concentration Assay

    Genistein effect on i Ca 2+ response to NMDA and ATP response. ( A ) ΔF/F 0 averaged response in Fluo-4-AM-labeled rCCA perfused with 1 mM NMDA in the presence of genistein (GX). ( B ) ΔF/F 0 averaged response in Fluo-4-AM-labeled rCCA perfused with ATP. Line above traces indicates perfusion with 1 mM NMDA after 120 sec basal recording. Line below (if applicable) indicates the inhibitor used throughout the recording time. ( C ) ∫(ΔF/F 0 ) distribution histogram for cell population responses in genistein and ATP conditions; the NMDA treated population distribution is included for comparison. The RTV value is indicated by the black arrowhead (see text). Statistical analysis for these distributions with the number of cells and the number of experiments are shown in Table 1 . Representative images from i Ca 2+ responses are shown in S8 Fig .

    Journal: PLoS ONE

    Article Title: A Metabotropic-Like Flux-Independent NMDA Receptor Regulates Ca2+ Exit from Endoplasmic Reticulum and Mitochondrial Membrane Potential in Cultured Astrocytes

    doi: 10.1371/journal.pone.0126314

    Figure Lengend Snippet: Genistein effect on i Ca 2+ response to NMDA and ATP response. ( A ) ΔF/F 0 averaged response in Fluo-4-AM-labeled rCCA perfused with 1 mM NMDA in the presence of genistein (GX). ( B ) ΔF/F 0 averaged response in Fluo-4-AM-labeled rCCA perfused with ATP. Line above traces indicates perfusion with 1 mM NMDA after 120 sec basal recording. Line below (if applicable) indicates the inhibitor used throughout the recording time. ( C ) ∫(ΔF/F 0 ) distribution histogram for cell population responses in genistein and ATP conditions; the NMDA treated population distribution is included for comparison. The RTV value is indicated by the black arrowhead (see text). Statistical analysis for these distributions with the number of cells and the number of experiments are shown in Table 1 . Representative images from i Ca 2+ responses are shown in S8 Fig .

    Article Snippet: Reagents and antibodies Salts, reagents, NMDA, adenosine tri-phosphate (ATP), D(-)-2-amino-5-phosphonopentanoic acid (APV), Kynurenic acid (KYNA), Dizocilpine (MK-801), XestosponginC (XesC), Ryanodine (Ry), Genistein (GX) and carbonyl cyanide 3-chlorophenylhydrazone (CCCP) were from Sigma Chemical Co. (St. Louis, MO, USA).

    Techniques: Labeling, Size-exclusion Chromatography