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Addgene inc addgene pkl ps113
Identification of CDK as an Upstream Kinase for <t>PKL</t> <t>pS113</t> (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p
Addgene Pkl Ps113, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production"

Article Title: Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production

Journal: Cell reports

doi: 10.1016/j.celrep.2019.11.009

Identification of CDK as an Upstream Kinase for PKL pS113 (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p
Figure Legend Snippet: Identification of CDK as an Upstream Kinase for PKL pS113 (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p

Techniques Used:

PKL S113 Is Phosphorylated in the Nucleus, Leading to Nuclear Retention and a Distinct Pool of PKL from the Cytosolic pS12 Population (A and B) Rat hepatoma cells (A) and primary hepatocytes (B) were immunostained for PKL pS113 and show strong nuclear localization (4‘,6-diamidino-2-phenylindole [DAPI]) of the pS113 signal. Scale bar represents 50 μm. (C–E) Rat hepatoma cells (C), primary hepatocytes (D), and samples from control, HFD, and PKCε ASO rat liver lysates (E) were fractionated into nuclear and cytosolic fractions, and then blotted for pS113, pS12, total PKL, total PKM2, and Histone H3 (as a nuclear marker). (F) Nuclear and cytosolic fractions from rat hepatoma cells treated overnight with DMSO or 40 μM importazole. (G) Model of PKL translocation and phosphorylation.
Figure Legend Snippet: PKL S113 Is Phosphorylated in the Nucleus, Leading to Nuclear Retention and a Distinct Pool of PKL from the Cytosolic pS12 Population (A and B) Rat hepatoma cells (A) and primary hepatocytes (B) were immunostained for PKL pS113 and show strong nuclear localization (4‘,6-diamidino-2-phenylindole [DAPI]) of the pS113 signal. Scale bar represents 50 μm. (C–E) Rat hepatoma cells (C), primary hepatocytes (D), and samples from control, HFD, and PKCε ASO rat liver lysates (E) were fractionated into nuclear and cytosolic fractions, and then blotted for pS113, pS12, total PKL, total PKM2, and Histone H3 (as a nuclear marker). (F) Nuclear and cytosolic fractions from rat hepatoma cells treated overnight with DMSO or 40 μM importazole. (G) Model of PKL translocation and phosphorylation.

Techniques Used: Allele-specific Oligonucleotide, Marker, Translocation Assay

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Article Title:
Article Snippet: .. Plasmids generated in this study have been deposited to Addgene: PKL pS113 and PKL pS12 antibodies generated in this study will be made available on request but we may require a payment and/or a completed Materials Transfer Agreement if there is potential for commercial application. ..

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    Addgene inc addgene pkl ps113
    Identification of CDK as an Upstream Kinase for <t>PKL</t> <t>pS113</t> (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p
    Addgene Pkl Ps113, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/addgene pkl ps113/product/Addgene inc
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    addgene pkl ps113 - by Bioz Stars, 2021-01
    92/100 stars
      Buy from Supplier

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    Identification of CDK as an Upstream Kinase for PKL pS113 (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p

    Journal: Cell reports

    Article Title: Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production

    doi: 10.1016/j.celrep.2019.11.009

    Figure Lengend Snippet: Identification of CDK as an Upstream Kinase for PKL pS113 (A) Primary hepatocytes treated with glucose and the gluconeogenic precursors pyruvate and lactate showed increased PKL pS113 compared to just glucose or glucose with the PKA activator IBMX. ), revealing a CDK-like motif (+1P, +3–5 K/R). Residues shaded in gray are fixed, and the size of the residue correlates with the enrichment of that residue at a position. Residues taller than the red lines represent statistical significance (p

    Article Snippet: Plasmids generated in this study have been deposited to Addgene: PKL pS113 and PKL pS12 antibodies generated in this study will be made available on request but we may require a payment and/or a completed Materials Transfer Agreement if there is potential for commercial application.

    Techniques:

    PKL S113 Is Phosphorylated in the Nucleus, Leading to Nuclear Retention and a Distinct Pool of PKL from the Cytosolic pS12 Population (A and B) Rat hepatoma cells (A) and primary hepatocytes (B) were immunostained for PKL pS113 and show strong nuclear localization (4‘,6-diamidino-2-phenylindole [DAPI]) of the pS113 signal. Scale bar represents 50 μm. (C–E) Rat hepatoma cells (C), primary hepatocytes (D), and samples from control, HFD, and PKCε ASO rat liver lysates (E) were fractionated into nuclear and cytosolic fractions, and then blotted for pS113, pS12, total PKL, total PKM2, and Histone H3 (as a nuclear marker). (F) Nuclear and cytosolic fractions from rat hepatoma cells treated overnight with DMSO or 40 μM importazole. (G) Model of PKL translocation and phosphorylation.

    Journal: Cell reports

    Article Title: Distinct Hepatic PKA and CDK Signaling Pathways Control Activity-Independent Pyruvate Kinase Phosphorylation and Hepatic Glucose Production

    doi: 10.1016/j.celrep.2019.11.009

    Figure Lengend Snippet: PKL S113 Is Phosphorylated in the Nucleus, Leading to Nuclear Retention and a Distinct Pool of PKL from the Cytosolic pS12 Population (A and B) Rat hepatoma cells (A) and primary hepatocytes (B) were immunostained for PKL pS113 and show strong nuclear localization (4‘,6-diamidino-2-phenylindole [DAPI]) of the pS113 signal. Scale bar represents 50 μm. (C–E) Rat hepatoma cells (C), primary hepatocytes (D), and samples from control, HFD, and PKCε ASO rat liver lysates (E) were fractionated into nuclear and cytosolic fractions, and then blotted for pS113, pS12, total PKL, total PKM2, and Histone H3 (as a nuclear marker). (F) Nuclear and cytosolic fractions from rat hepatoma cells treated overnight with DMSO or 40 μM importazole. (G) Model of PKL translocation and phosphorylation.

    Article Snippet: Plasmids generated in this study have been deposited to Addgene: PKL pS113 and PKL pS12 antibodies generated in this study will be made available on request but we may require a payment and/or a completed Materials Transfer Agreement if there is potential for commercial application.

    Techniques: Allele-specific Oligonucleotide, Marker, Translocation Assay