adcc assay human peripheral blood mononuclear cells pbmc  (AllCells LLC)

 
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    Structured Review

    AllCells LLC adcc assay human peripheral blood mononuclear cells pbmc
    <t>ADCC</t> of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of <t>PBMC</t> effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.
    Adcc Assay Human Peripheral Blood Mononuclear Cells Pbmc, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adcc assay human peripheral blood mononuclear cells pbmc/product/AllCells LLC
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    adcc assay human peripheral blood mononuclear cells pbmc - by Bioz Stars, 2020-09
    85/100 stars

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    rpmi1640 medium

    Images

    1) Product Images from "Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey"

    Article Title: Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0196422

    ADCC of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of PBMC effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.
    Figure Legend Snippet: ADCC of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of PBMC effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.

    Techniques Used: Multiple Displacement Amplification, Labeling, Incubation

    2) Product Images from "Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers"

    Article Title: Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers

    Journal: Molecular Cancer

    doi: 10.1186/1476-4598-12-60

    ADCC of anti-Nectin-2 mAbs against OV-90 cells. OV-90 cells pre-labeled with 51 Cr were incubated with anti-Nectin-2 mAbs at a ratio of 1:50 with PBMC effector cells for 4 h at 37°C, followed by the measurement of 51 Cr that was released into the culture supernatant. Specific cell lysis was calculated as described in Methods. The numbers in parentheses indicate the epitope bin of each antibody. The results are the mean ± S.D. of triplicate assays.
    Figure Legend Snippet: ADCC of anti-Nectin-2 mAbs against OV-90 cells. OV-90 cells pre-labeled with 51 Cr were incubated with anti-Nectin-2 mAbs at a ratio of 1:50 with PBMC effector cells for 4 h at 37°C, followed by the measurement of 51 Cr that was released into the culture supernatant. Specific cell lysis was calculated as described in Methods. The numbers in parentheses indicate the epitope bin of each antibody. The results are the mean ± S.D. of triplicate assays.

    Techniques Used: Labeling, Incubation, Lysis

    ADCC of Y-443 and its IgG 4 against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with 51 Cr were incubated with Y-443 (IgG 1 ), Y-443 IgG 4 , or control human IgG at a ratio of 1:50 with PBMC effector cells for 4 h at 37°C, followed by measurement of 51 Cr that was released into the culture supernatant. Specific cell lysis was calculated as described in Methods. The results are the mean ± S.D. of triplicate assays.
    Figure Legend Snippet: ADCC of Y-443 and its IgG 4 against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with 51 Cr were incubated with Y-443 (IgG 1 ), Y-443 IgG 4 , or control human IgG at a ratio of 1:50 with PBMC effector cells for 4 h at 37°C, followed by measurement of 51 Cr that was released into the culture supernatant. Specific cell lysis was calculated as described in Methods. The results are the mean ± S.D. of triplicate assays.

    Techniques Used: Multiple Displacement Amplification, Labeling, Incubation, Lysis

    Related Articles

    Multiple Displacement Amplification:

    Article Title: Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers
    Article Snippet: .. ADCC assay Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2-mercaptoethanol for 24 h. PBMC were incubated with 51 Cr-labeled OV-90 cells or 51 Cr-labeled MDA-MB-231 cells at a ratio of 50:1 in the presence of anti-Nectin-2 mAb for 4 h at 37°C. .. After incubation, the radioactivity of 51 Cr that had been released into the culture medium from the target cells was measured by using an AccuFLEX γ 7000 (Aloka).

    Incubation:

    Article Title: Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers
    Article Snippet: .. ADCC assay Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2-mercaptoethanol for 24 h. PBMC were incubated with 51 Cr-labeled OV-90 cells or 51 Cr-labeled MDA-MB-231 cells at a ratio of 50:1 in the presence of anti-Nectin-2 mAb for 4 h at 37°C. .. After incubation, the radioactivity of 51 Cr that had been released into the culture medium from the target cells was measured by using an AccuFLEX γ 7000 (Aloka).

    ADCC Assay:

    Article Title: Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey
    Article Snippet: .. ADCC assay Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 (DIACLONE Research) and 55 μM 2-mercaptoethanol for 24 hours. .. PBMC were incubated with Calcein AM-labeled MDA-MB-231 cells at a ratio of 50:1 in the presence of anti-Nectin-2 mAb for 4 hours at 37°C.

    Article Title: Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers
    Article Snippet: .. ADCC assay Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2-mercaptoethanol for 24 h. PBMC were incubated with 51 Cr-labeled OV-90 cells or 51 Cr-labeled MDA-MB-231 cells at a ratio of 50:1 in the presence of anti-Nectin-2 mAb for 4 h at 37°C. .. After incubation, the radioactivity of 51 Cr that had been released into the culture medium from the target cells was measured by using an AccuFLEX γ 7000 (Aloka).

    Cell Culture:

    Article Title: Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey
    Article Snippet: .. ADCC assay Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 (DIACLONE Research) and 55 μM 2-mercaptoethanol for 24 hours. .. PBMC were incubated with Calcein AM-labeled MDA-MB-231 cells at a ratio of 50:1 in the presence of anti-Nectin-2 mAb for 4 hours at 37°C.

    Article Title: Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers
    Article Snippet: .. ADCC assay Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2-mercaptoethanol for 24 h. PBMC were incubated with 51 Cr-labeled OV-90 cells or 51 Cr-labeled MDA-MB-231 cells at a ratio of 50:1 in the presence of anti-Nectin-2 mAb for 4 h at 37°C. .. After incubation, the radioactivity of 51 Cr that had been released into the culture medium from the target cells was measured by using an AccuFLEX γ 7000 (Aloka).

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  • 85
    AllCells LLC adcc assay human peripheral blood mononuclear cells pbmc
    <t>ADCC</t> of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of <t>PBMC</t> effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.
    Adcc Assay Human Peripheral Blood Mononuclear Cells Pbmc, supplied by AllCells LLC, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adcc assay human peripheral blood mononuclear cells pbmc/product/AllCells LLC
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    adcc assay human peripheral blood mononuclear cells pbmc - by Bioz Stars, 2020-09
    85/100 stars
      Buy from Supplier

    Image Search Results


    ADCC of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of PBMC effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.

    Journal: PLoS ONE

    Article Title: Fc engineering of anti-Nectin-2 antibody improved thrombocytopenic adverse event in monkey

    doi: 10.1371/journal.pone.0196422

    Figure Lengend Snippet: ADCC of Y-443 and Fc-mutated antibodies against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with Calcein AM were incubated with different concentrations of Y-443 or the Fc mutants, followed by addition of PBMC effector cells at a ratio of 1:50. The cell mixture was incubated for 4 hours at 37°C, and Calcein AM intensity in cells was detected by Acumen eX3 (TTP labtech). The results are the mean ± S.D. of dead cell ratio.

    Article Snippet: ADCC assay Human peripheral blood mononuclear cells (PBMC) purchased from AllCells, LLC and were cultured in RPMI1640 medium containing 10% fetal bovine serum, 0.1 nM human IL-2 (DIACLONE Research) and 55 μM 2-mercaptoethanol for 24 hours.

    Techniques: Multiple Displacement Amplification, Labeling, Incubation

    ADCC of anti-Nectin-2 mAbs against OV-90 cells. OV-90 cells pre-labeled with 51 Cr were incubated with anti-Nectin-2 mAbs at a ratio of 1:50 with PBMC effector cells for 4 h at 37°C, followed by the measurement of 51 Cr that was released into the culture supernatant. Specific cell lysis was calculated as described in Methods. The numbers in parentheses indicate the epitope bin of each antibody. The results are the mean ± S.D. of triplicate assays.

    Journal: Molecular Cancer

    Article Title: Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers

    doi: 10.1186/1476-4598-12-60

    Figure Lengend Snippet: ADCC of anti-Nectin-2 mAbs against OV-90 cells. OV-90 cells pre-labeled with 51 Cr were incubated with anti-Nectin-2 mAbs at a ratio of 1:50 with PBMC effector cells for 4 h at 37°C, followed by the measurement of 51 Cr that was released into the culture supernatant. Specific cell lysis was calculated as described in Methods. The numbers in parentheses indicate the epitope bin of each antibody. The results are the mean ± S.D. of triplicate assays.

    Article Snippet: ADCC assay Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2-mercaptoethanol for 24 h. PBMC were incubated with 51 Cr-labeled OV-90 cells or 51 Cr-labeled MDA-MB-231 cells at a ratio of 50:1 in the presence of anti-Nectin-2 mAb for 4 h at 37°C.

    Techniques: Labeling, Incubation, Lysis

    ADCC of Y-443 and its IgG 4 against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with 51 Cr were incubated with Y-443 (IgG 1 ), Y-443 IgG 4 , or control human IgG at a ratio of 1:50 with PBMC effector cells for 4 h at 37°C, followed by measurement of 51 Cr that was released into the culture supernatant. Specific cell lysis was calculated as described in Methods. The results are the mean ± S.D. of triplicate assays.

    Journal: Molecular Cancer

    Article Title: Nectin-2 is a potential target for antibody therapy of breast and ovarian cancers

    doi: 10.1186/1476-4598-12-60

    Figure Lengend Snippet: ADCC of Y-443 and its IgG 4 against MDA-MB-231 cells. MDA-MB-231 cells pre-labeled with 51 Cr were incubated with Y-443 (IgG 1 ), Y-443 IgG 4 , or control human IgG at a ratio of 1:50 with PBMC effector cells for 4 h at 37°C, followed by measurement of 51 Cr that was released into the culture supernatant. Specific cell lysis was calculated as described in Methods. The results are the mean ± S.D. of triplicate assays.

    Article Snippet: ADCC assay Human peripheral blood mononuclear cells (PBMC) from AllCells were cultured in RPMI1640 medium containing 10% FBS, 0.1 nM human IL-2 (DIACLONE Research), and 55 μM 2-mercaptoethanol for 24 h. PBMC were incubated with 51 Cr-labeled OV-90 cells or 51 Cr-labeled MDA-MB-231 cells at a ratio of 50:1 in the presence of anti-Nectin-2 mAb for 4 h at 37°C.

    Techniques: Multiple Displacement Amplification, Labeling, Incubation, Lysis