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Illumina Inc adaptor oligos
Adaptor Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adaptor oligos/product/Illumina Inc
Average 93 stars, based on 3 article reviews
Price from $9.99 to $1999.99
adaptor oligos - by Bioz Stars, 2020-04
93/100 stars

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Related Articles

Functional Assay:

Article Title: HNF6 and Rev-erbα integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Article Snippet: DNA was amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and a PCR purification kit and MinElute kit from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core of the Pennsylvania Diabetes Research Center using the Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core (J. Schug and K. Kaestner) of the Penn Diabetes Research Center using the Illumina Genome Analyzer IIx and Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Amplification:

Article Title: Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge
Article Snippet: .. Adaptor oligos and primer sequences from Illumina were utilized for library construction and amplification. .. Prior to PCR library amplification, size selection of adaptor ligated DNA was performed using Agencourt AMPure XP Beads (Beckman Coulter).

Article Title: HNF6 and Rev-erbα integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Article Snippet: .. DNA was amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and a PCR purification kit and MinElute kit from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core of the Pennsylvania Diabetes Research Center using the Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: .. The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core (J. Schug and K. Kaestner) of the Penn Diabetes Research Center using the Illumina Genome Analyzer IIx and Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Sample Prep:

Article Title: Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge
Article Snippet: Enzymes from New England Biolabs were used to generate libraries according to the ChIP Sequencing Sample Preparation Guide provided by Illumina. .. Adaptor oligos and primer sequences from Illumina were utilized for library construction and amplification.

Article Title: HNF6 and Rev-erbα integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Article Snippet: .. DNA was amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and a PCR purification kit and MinElute kit from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core of the Pennsylvania Diabetes Research Center using the Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: .. The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core (J. Schug and K. Kaestner) of the Penn Diabetes Research Center using the Illumina Genome Analyzer IIx and Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Polymerase Chain Reaction:

Article Title: Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge
Article Snippet: Adaptor oligos and primer sequences from Illumina were utilized for library construction and amplification. .. Prior to PCR library amplification, size selection of adaptor ligated DNA was performed using Agencourt AMPure XP Beads (Beckman Coulter).

Article Title: HNF6 and Rev-erbα integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Article Snippet: .. DNA was amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and a PCR purification kit and MinElute kit from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core of the Pennsylvania Diabetes Research Center using the Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: .. The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core (J. Schug and K. Kaestner) of the Penn Diabetes Research Center using the Illumina Genome Analyzer IIx and Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Mutagenesis:

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: The lin-4 transgenic strains were derived by segregating lines of transgene-carrying animals homozygous for the chromosomal lin-4 (e912) mutation from the lin-4 (e912)/mIn1 transgenic lines created from the injection of lin4_m constructs. .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ).

Isolation:

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: Small RNAs were isolated from these frozen pellets using the mirVana Isolation Kit (Ambion) and used for library preparation following the 5′-monophosphate method that enriches for microRNAs, as previously described ( ). .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ).

Construct:

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: The let-7 transgenic strains were derived by isolating let-7 (mn112) unc-3(e151) animals (uncoordinated animals expressing p mec-7::gfp ) from the mnDp1/+; let-7 (mn112) unc-3(e151) transgenic lines created from the injection of let7_m constructs. .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ).

Purification:

Article Title: Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge
Article Snippet: Adaptor oligos and primer sequences from Illumina were utilized for library construction and amplification. .. PCR Purification and MinElute (QIAGEN) kits were used for library purification steps.

Article Title: HNF6 and Rev-erbα integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Article Snippet: .. DNA was amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and a PCR purification kit and MinElute kit from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core of the Pennsylvania Diabetes Research Center using the Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: .. The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core (J. Schug and K. Kaestner) of the Penn Diabetes Research Center using the Illumina Genome Analyzer IIx and Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Sequencing:

Article Title: Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge
Article Snippet: Enzymes from New England Biolabs were used to generate libraries according to the ChIP Sequencing Sample Preparation Guide provided by Illumina. .. Adaptor oligos and primer sequences from Illumina were utilized for library construction and amplification.

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ). .. Thirty-six nucleotide reads were generated from the small RNA libraries using the Illumina Genome Analyzer system.

Article Title: HNF6 and Rev-erbα integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Article Snippet: .. DNA was amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and a PCR purification kit and MinElute kit from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core of the Pennsylvania Diabetes Research Center using the Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: .. The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core (J. Schug and K. Kaestner) of the Penn Diabetes Research Center using the Illumina Genome Analyzer IIx and Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Transgenic Assay:

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: The let-7 transgenic strains were derived by isolating let-7 (mn112) unc-3(e151) animals (uncoordinated animals expressing p mec-7::gfp ) from the mnDp1/+; let-7 (mn112) unc-3(e151) transgenic lines created from the injection of let7_m constructs. .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ).

Selection:

Article Title: Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge
Article Snippet: Adaptor oligos and primer sequences from Illumina were utilized for library construction and amplification. .. Prior to PCR library amplification, size selection of adaptor ligated DNA was performed using Agencourt AMPure XP Beads (Beckman Coulter).

ChIP-sequencing:

Article Title: Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge
Article Snippet: Paragraph title: ChIP-seq library preparation ... Adaptor oligos and primer sequences from Illumina were utilized for library construction and amplification.

Article Title: HNF6 and Rev-erbα integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Article Snippet: Paragraph title: ChIP-seq ... DNA was amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and a PCR purification kit and MinElute kit from Qiagen.

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: Paragraph title: ChIP-seq ... The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen.

Expressing:

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: The let-7 transgenic strains were derived by isolating let-7 (mn112) unc-3(e151) animals (uncoordinated animals expressing p mec-7::gfp ) from the mnDp1/+; let-7 (mn112) unc-3(e151) transgenic lines created from the injection of let7_m constructs. .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ).

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen. .. All data are available in Gene Expression Omnibus (GEO) under accession number , and all previously published data ( ) are available under accession number .

Modification:

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ). .. Thirty-six nucleotide reads were generated from the small RNA libraries using the Illumina Genome Analyzer system.

Injection:

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: The let-7 transgenic strains were derived by isolating let-7 (mn112) unc-3(e151) animals (uncoordinated animals expressing p mec-7::gfp ) from the mnDp1/+; let-7 (mn112) unc-3(e151) transgenic lines created from the injection of let7_m constructs. .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ).

Mouse Assay:

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: ChIP experiments were performed independently on liver samples from four different mice harvested at 5:00 a.m. or 5:00 p.m. ChIP of Rev-erbα in Rev-erbα-null mice was performed using the Cell Signaling Technology antibody (#2124). .. The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen.

Chromatin Immunoprecipitation:

Article Title: Histone Deacetylase 3 Prepares Brown Adipose Tissue For Acute Thermogenic Challenge
Article Snippet: Enzymes from New England Biolabs were used to generate libraries according to the ChIP Sequencing Sample Preparation Guide provided by Illumina. .. Adaptor oligos and primer sequences from Illumina were utilized for library construction and amplification.

Article Title: HNF6 and Rev-erbα integrate hepatic lipid metabolism by overlapping and distinct transcriptional mechanisms
Article Snippet: .. DNA was amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and a PCR purification kit and MinElute kit from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core of the Pennsylvania Diabetes Research Center using the Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Article Title: Rev-erb? and Rev-erb? coordinately protect the circadian clock and normal metabolic function
Article Snippet: .. The precipitated DNA was subsequently pooled and amplified according to the ChIP Sequencing Sample Preparation Guide provided by Illumina using adaptor oligos and primers from Illumina, enzymes from New England Biolabs, and the PCR purification kit (#28104) and MinElute kit (#28004) from Qiagen. .. Deep sequencing was performed by the Functional Genomics Core (J. Schug and K. Kaestner) of the Penn Diabetes Research Center using the Illumina Genome Analyzer IIx and Illumina HiSeq 2000, and sequences were obtained using the Solexa Analysis Pipeline.

Derivative Assay:

Article Title: Functional relevance of “seed” and “non-seed” sequences in microRNA-mediated promotion of C. elegans developmental progression
Article Snippet: The let-7 transgenic strains were derived by isolating let-7 (mn112) unc-3(e151) animals (uncoordinated animals expressing p mec-7::gfp ) from the mnDp1/+; let-7 (mn112) unc-3(e151) transgenic lines created from the injection of let7_m constructs. .. The exception was that modified 5′-adaptor oligonucleotides, which included 4-nt barcodes, were utilized to enable pooling of several libraries for Illumina sequencing ( ).

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    Illumina Inc pcr primer adaptors
    Schematic diagram of the methylated CpG island amplification microarray (MCAM) method. Enrichment for methylated <t>DNA</t> and reduction of genome complexity is achieved by serial digestion with Sma I (methylation sensitive) and Xma I (methylation insensitive) restriction enzymes, followed by ligation of adaptors and <t>PCR</t> amplification. The resulting amplicons, representative of the methylated fraction of tumor and normal cells, are labeled and co-hybridized in a microarray platform. Image acquisition and data analysis allow identification of methylated and non-methylated genes by comparing intensity values of Cy5 and Cy3 dyes for each pair of tumor and control samples. In this example, the M - A plot of normalized data from the cancer cell line MDA-MB-435 compared to normal peripheral blood is presented, from which amplicons were co-hybridized to a custom Agilent microarray containing 44,000 olinucleotide probes targeting human promoter CpG islands.
    Pcr Primer Adaptors, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer adaptors/product/Illumina Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr primer adaptors - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    96
    Illumina Inc illumina adaptor primer
    Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with <t>Illumina</t> adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.
    Illumina Adaptor Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina adaptor primer/product/Illumina Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    illumina adaptor primer - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    94
    Illumina Inc paired end adaptor oligonucleotides
    Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with <t>Illumina</t> adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.
    Paired End Adaptor Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paired end adaptor oligonucleotides/product/Illumina Inc
    Average 94 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    paired end adaptor oligonucleotides - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    85
    Illumina Inc rna dna chimeric oligonucleotide adaptors
    Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with <t>Illumina</t> adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.
    Rna Dna Chimeric Oligonucleotide Adaptors, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna dna chimeric oligonucleotide adaptors/product/Illumina Inc
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rna dna chimeric oligonucleotide adaptors - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results


    Schematic diagram of the methylated CpG island amplification microarray (MCAM) method. Enrichment for methylated DNA and reduction of genome complexity is achieved by serial digestion with Sma I (methylation sensitive) and Xma I (methylation insensitive) restriction enzymes, followed by ligation of adaptors and PCR amplification. The resulting amplicons, representative of the methylated fraction of tumor and normal cells, are labeled and co-hybridized in a microarray platform. Image acquisition and data analysis allow identification of methylated and non-methylated genes by comparing intensity values of Cy5 and Cy3 dyes for each pair of tumor and control samples. In this example, the M - A plot of normalized data from the cancer cell line MDA-MB-435 compared to normal peripheral blood is presented, from which amplicons were co-hybridized to a custom Agilent microarray containing 44,000 olinucleotide probes targeting human promoter CpG islands.

    Journal: Genome Medicine

    Article Title: Tackling the methylome: recent methodological advances in genome-wide methylation profiling

    doi: 10.1186/gm106

    Figure Lengend Snippet: Schematic diagram of the methylated CpG island amplification microarray (MCAM) method. Enrichment for methylated DNA and reduction of genome complexity is achieved by serial digestion with Sma I (methylation sensitive) and Xma I (methylation insensitive) restriction enzymes, followed by ligation of adaptors and PCR amplification. The resulting amplicons, representative of the methylated fraction of tumor and normal cells, are labeled and co-hybridized in a microarray platform. Image acquisition and data analysis allow identification of methylated and non-methylated genes by comparing intensity values of Cy5 and Cy3 dyes for each pair of tumor and control samples. In this example, the M - A plot of normalized data from the cancer cell line MDA-MB-435 compared to normal peripheral blood is presented, from which amplicons were co-hybridized to a custom Agilent microarray containing 44,000 olinucleotide probes targeting human promoter CpG islands.

    Article Snippet: To do this, two groups fragmented the genomic DNA by sonication prior to ligation of PCR primer adaptors and bisulfite conversion, and performed shotgun sequencing using the Illumina Solexa platform.

    Techniques: Methylation, Amplification, Microarray, Ligation, Polymerase Chain Reaction, Labeling, Multiple Displacement Amplification

    Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with Illumina adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.

    Journal: Nucleic Acids Research

    Article Title: Simple, multiplexed, PCR-based barcoding of DNA enables sensitive mutation detection in liquid biopsies using sequencing

    doi: 10.1093/nar/gkw224

    Figure Lengend Snippet: Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with Illumina adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.

    Article Snippet: The second round of PCR was performed in 40 μl using 1× Q5 Hot Start High-Fidelity Master Mix (New England BioLabs), 400 nM of each Illumina adaptor primer and 10 μl PCR products from the first round of PCR.

    Techniques: Polymerase Chain Reaction, Amplification, Incubation, Purification, Magnetic Beads, Concentration Assay