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Illumina Inc adapter oligos
Adapter Oligos, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adapter oligos/product/Illumina Inc
Average 96 stars, based on 2 article reviews
Price from $9.99 to $1999.99
adapter oligos - by Bioz Stars, 2020-04
96/100 stars

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High Performance Liquid Chromatography:

Article Title: Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications
Article Snippet: .. Alternatively, adapter oligos and PCR primers compatible with Illumina sequencing can be purchased elsewhere; HPLC purification is recommended. .. The paired end DNA oligonucleotides are more universal since the resulting library can be sequenced with either single end or paired end sequencing primers.

Real-time Polymerase Chain Reaction:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: DNA fragments were analyzed by qPCR using SYBR Green Master Mix (Kapa Biosciences) on an ABI 7300 instrument (Applied Biosystems). .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified.

Amplification:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified. .. Sequencing was performed at the Center for Cancer Computational Biology at Dana Farber Cancer Institute using an Illumina HiSeq2000 instrument.

Ligation:

Article Title: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing
Article Snippet: Adapter oligonucleotides from Illumina (single reads) were ligated to the ends. .. Subsequently, ligation was confirmed by four-cycle PCR using a high-fidelity polymerase with primers containing a custom-synthesized barcode sequence (8 bp) as a sample index signature.

Isolation:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: DNA was isolated using PCR Cleanup Kit (Qiagen) and quantitated using Quant-IT as per the manufacturer’s instructions (Thermo Scientific). .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified.

Next-Generation Sequencing:

Article Title: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing
Article Snippet: Paragraph title: Targeted capturing and next generation sequencing ... Adapter oligonucleotides from Illumina (single reads) were ligated to the ends.

Incubation:

Article Title: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing
Article Snippet: According to standard Illumina protocols, terminal A residues were added following a brief incubation with the Klenow 3′-5′ exo-enzyme and dATP. .. Adapter oligonucleotides from Illumina (single reads) were ligated to the ends.

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: Chromatin complexes were immunoprecipitated by an overnight incubation at 4 °C with 2 µg anti-ER antibodies (Santa Cruz, HC-20 and Thermo Fisher, AB-10), followed by a 45 min incubation with 40 µl 1:1 mix of protein A and G Dynabeads (Invitrogen). .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified.

Purification:

Article Title: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing
Article Snippet: Genomic DNA was fragmented ranging from 200 bp to 250 bp and purified, followed by treatment with T4 DNA polymerase, T4 phosphonucleotide kinase and Klenow fragment of DNA polymerase to fill 5′ overhangs and to remove 3′ overhangs. .. Adapter oligonucleotides from Illumina (single reads) were ligated to the ends.

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified. .. Sequencing was performed at the Center for Cancer Computational Biology at Dana Farber Cancer Institute using an Illumina HiSeq2000 instrument.

Article Title: Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications
Article Snippet: .. Alternatively, adapter oligos and PCR primers compatible with Illumina sequencing can be purchased elsewhere; HPLC purification is recommended. .. The paired end DNA oligonucleotides are more universal since the resulting library can be sequenced with either single end or paired end sequencing primers.

SYBR Green Assay:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: DNA fragments were analyzed by qPCR using SYBR Green Master Mix (Kapa Biosciences) on an ABI 7300 instrument (Applied Biosystems). .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified.

Immunoprecipitation:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: Chromatin complexes were immunoprecipitated by an overnight incubation at 4 °C with 2 µg anti-ER antibodies (Santa Cruz, HC-20 and Thermo Fisher, AB-10), followed by a 45 min incubation with 40 µl 1:1 mix of protein A and G Dynabeads (Invitrogen). .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified.

Generated:

Article Title: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing
Article Snippet: Adapter oligonucleotides from Illumina (single reads) were ligated to the ends. .. PCR generated a library for further analysis, and the indexed fragments and DNA adapter-ligated were pooled and hybridized to the capture array.

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified. .. ChIP-seq SPMR (signal per million reads) traces were generated using MACS2.1 followed by conversion to BigWig format using the bedGraphToBigWig tool.

Polymerase Chain Reaction:

Article Title: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing
Article Snippet: Adapter oligonucleotides from Illumina (single reads) were ligated to the ends. .. Subsequently, ligation was confirmed by four-cycle PCR using a high-fidelity polymerase with primers containing a custom-synthesized barcode sequence (8 bp) as a sample index signature.

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: DNA was isolated using PCR Cleanup Kit (Qiagen) and quantitated using Quant-IT as per the manufacturer’s instructions (Thermo Scientific). .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified.

Article Title: Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications
Article Snippet: .. Alternatively, adapter oligos and PCR primers compatible with Illumina sequencing can be purchased elsewhere; HPLC purification is recommended. .. The paired end DNA oligonucleotides are more universal since the resulting library can be sequenced with either single end or paired end sequencing primers.

Sequencing:

Article Title: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing
Article Snippet: Adapter oligonucleotides from Illumina (single reads) were ligated to the ends. .. Subsequently, ligation was confirmed by four-cycle PCR using a high-fidelity polymerase with primers containing a custom-synthesized barcode sequence (8 bp) as a sample index signature.

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified. .. Sequencing was performed at the Center for Cancer Computational Biology at Dana Farber Cancer Institute using an Illumina HiSeq2000 instrument.

Article Title: Using ChIP-Seq Technology to Generate High-Resolution Profiles of Histone Modifications
Article Snippet: .. Alternatively, adapter oligos and PCR primers compatible with Illumina sequencing can be purchased elsewhere; HPLC purification is recommended. .. The paired end DNA oligonucleotides are more universal since the resulting library can be sequenced with either single end or paired end sequencing primers.

Lysis:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: Beads were then washed four times with RIPA lysis buffer (50 mM HEPES, 1 mM EDTA, 0.7% deoxycholate, 1% NP-40, and 0.5 M LiCl) and twice with Tris-EDTA (TE) buffer. .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified.

Binding Assay:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified. .. Reads were mapped to the human genome using STAR and peaks indicating ER binding were called comparing ChIP and corresponding Chromatin Input libraries using MACS2.1 and a threshold of p < 10−5 .

Chromatin Immunoprecipitation:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: Paragraph title: ChIP assay ... For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified.

ChIP-sequencing:

Article Title: The actin cytoskeletal architecture of estrogen receptor positive breast cancer cells suppresses invasion
Article Snippet: .. For ChIP-seq, purified DNA was then end repaired and ligated to adapter oligos (Illumina) and amplified. .. Sequencing was performed at the Center for Cancer Computational Biology at Dana Farber Cancer Institute using an Illumina HiSeq2000 instrument.

Hybridization:

Article Title: Novel mutations identified in Chinese families with autosomal dominant congenital cataracts by targeted next-generation sequencing
Article Snippet: Adapter oligonucleotides from Illumina (single reads) were ligated to the ends. .. After hybridization and enrichment, the DNA sample was sequenced on Illumina HiSeq2000 Analyzers to generate paired end reads (90 bps) [ ].

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  • 86
    Illumina Inc pcr primer adaptors
    Schematic diagram of the methylated CpG island amplification microarray (MCAM) method. Enrichment for methylated <t>DNA</t> and reduction of genome complexity is achieved by serial digestion with Sma I (methylation sensitive) and Xma I (methylation insensitive) restriction enzymes, followed by ligation of adaptors and <t>PCR</t> amplification. The resulting amplicons, representative of the methylated fraction of tumor and normal cells, are labeled and co-hybridized in a microarray platform. Image acquisition and data analysis allow identification of methylated and non-methylated genes by comparing intensity values of Cy5 and Cy3 dyes for each pair of tumor and control samples. In this example, the M - A plot of normalized data from the cancer cell line MDA-MB-435 compared to normal peripheral blood is presented, from which amplicons were co-hybridized to a custom Agilent microarray containing 44,000 olinucleotide probes targeting human promoter CpG islands.
    Pcr Primer Adaptors, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr primer adaptors/product/Illumina Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pcr primer adaptors - by Bioz Stars, 2020-04
    86/100 stars
      Buy from Supplier

    96
    Illumina Inc illumina adaptor primer
    Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with <t>Illumina</t> adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.
    Illumina Adaptor Primer, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/illumina adaptor primer/product/Illumina Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    illumina adaptor primer - by Bioz Stars, 2020-04
    96/100 stars
      Buy from Supplier

    94
    Illumina Inc paired end adaptor oligonucleotides
    Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with <t>Illumina</t> adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.
    Paired End Adaptor Oligonucleotides, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paired end adaptor oligonucleotides/product/Illumina Inc
    Average 94 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    paired end adaptor oligonucleotides - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

    85
    Illumina Inc rna dna chimeric oligonucleotide adaptors
    Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with <t>Illumina</t> adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.
    Rna Dna Chimeric Oligonucleotide Adaptors, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rna dna chimeric oligonucleotide adaptors/product/Illumina Inc
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    rna dna chimeric oligonucleotide adaptors - by Bioz Stars, 2020-04
    85/100 stars
      Buy from Supplier

    Image Search Results


    Schematic diagram of the methylated CpG island amplification microarray (MCAM) method. Enrichment for methylated DNA and reduction of genome complexity is achieved by serial digestion with Sma I (methylation sensitive) and Xma I (methylation insensitive) restriction enzymes, followed by ligation of adaptors and PCR amplification. The resulting amplicons, representative of the methylated fraction of tumor and normal cells, are labeled and co-hybridized in a microarray platform. Image acquisition and data analysis allow identification of methylated and non-methylated genes by comparing intensity values of Cy5 and Cy3 dyes for each pair of tumor and control samples. In this example, the M - A plot of normalized data from the cancer cell line MDA-MB-435 compared to normal peripheral blood is presented, from which amplicons were co-hybridized to a custom Agilent microarray containing 44,000 olinucleotide probes targeting human promoter CpG islands.

    Journal: Genome Medicine

    Article Title: Tackling the methylome: recent methodological advances in genome-wide methylation profiling

    doi: 10.1186/gm106

    Figure Lengend Snippet: Schematic diagram of the methylated CpG island amplification microarray (MCAM) method. Enrichment for methylated DNA and reduction of genome complexity is achieved by serial digestion with Sma I (methylation sensitive) and Xma I (methylation insensitive) restriction enzymes, followed by ligation of adaptors and PCR amplification. The resulting amplicons, representative of the methylated fraction of tumor and normal cells, are labeled and co-hybridized in a microarray platform. Image acquisition and data analysis allow identification of methylated and non-methylated genes by comparing intensity values of Cy5 and Cy3 dyes for each pair of tumor and control samples. In this example, the M - A plot of normalized data from the cancer cell line MDA-MB-435 compared to normal peripheral blood is presented, from which amplicons were co-hybridized to a custom Agilent microarray containing 44,000 olinucleotide probes targeting human promoter CpG islands.

    Article Snippet: To do this, two groups fragmented the genomic DNA by sonication prior to ligation of PCR primer adaptors and bisulfite conversion, and performed shotgun sequencing using the Illumina Solexa platform.

    Techniques: Methylation, Amplification, Microarray, Ligation, Polymerase Chain Reaction, Labeling, Multiple Displacement Amplification

    Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with Illumina adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.

    Journal: Nucleic Acids Research

    Article Title: Simple, multiplexed, PCR-based barcoding of DNA enables sensitive mutation detection in liquid biopsies using sequencing

    doi: 10.1093/nar/gkw224

    Figure Lengend Snippet: Schematic library construction workflow. In the first PCR consisting of three cycles, target DNA is amplified with hairpin protected barcode primers. The reaction is terminated with an incubation step that is a combined dilution and protease treatment step. In the second PCR that consists of 18–30 cycles, all individual amplicons are amplified to generate PCR products with Illumina adapter primers. Final libraries are purified with magnetic beads, normalized for concentration differences between samples and sequenced.

    Article Snippet: The second round of PCR was performed in 40 μl using 1× Q5 Hot Start High-Fidelity Master Mix (New England BioLabs), 400 nM of each Illumina adaptor primer and 10 μl PCR products from the first round of PCR.

    Techniques: Polymerase Chain Reaction, Amplification, Incubation, Purification, Magnetic Beads, Concentration Assay