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Illumina Inc adapter 2
Adapter 2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/adapter 2/product/Illumina Inc
Average 99 stars, based on 6 article reviews
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adapter 2 - by Bioz Stars, 2019-10
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Related Articles

Amplification:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library. .. The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library.

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library. .. After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library.

Article Title: Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål
Article Snippet: After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags. .. After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags.

Article Title: RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens
Article Snippet: After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags. .. After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags.

Article Title: Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent
Article Snippet: After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments. .. After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends. .. A 15 cycle PCR was performed with two primers (primer GX1 and primer GX2) that anneal to the ends of the adapters to enrich the adapter-ligated cDNA construct.

Article Title: DeepSAGE Based Differential Gene Expression Analysis under Cold and Freeze Stress in Seabuckthorn (Hippophae rhamnoides L.)
Article Snippet: Illumina adapter 2 was ligated to the 3’ ends of the tags to generate a tag library by acquiring different adaptors at both ends. .. Illumina adapter 2 was ligated to the 3’ ends of the tags to generate a tag library by acquiring different adaptors at both ends.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Synthesized:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: First strand cDNA was synthesized with oligo (dT) on the bead. .. The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library.

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: First- and second-strand cDNA was synthesized, and bead-bound cDNA was subsequently digested with NlaIII, which recognizes CATG sites. .. After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library.

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: First- and second-strand cDNA was synthesized and then digested with the restriction enzyme NlaIII, digesting 3′ of CATG sites. .. Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end.

Article Title: Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål
Article Snippet: RNA quality was evaluated using a 2100 Bioanalyzer (Agilent) according to the Illumina instructions. mRNA was isolated from 6 μg of total RNA from each sample using oligo (dT) magnetic beads, and then double-stranded cDNA was synthesized after reverse transcription using oligo (dT) primers bound to magnetic beads. .. After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags.

Article Title: RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens
Article Snippet: For BPH, mRNA was isolated form 6 μg of total RNA from each sample using oligo (dT) magnetic beads, and then double-stranded cDNA was synthesized after reverse transcription using oligo (dT) primer bound to magnetic beads. .. After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: Double strand cDNA were synthesized using Oligo dT beads. .. The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends.

Article Title: Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd)
Article Snippet: Double-stranded cDNA was directly synthesized on the beads and subsequently digested with NlaIII. .. The Illumina adapter 2 was then ligated to the 3′ ends of the tag.

Article Title: Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Article Snippet: First- and second-strand cDNA was synthesized. .. Illumina adapter 2 was ligated to the 3′-end of the cDNA tag after removing the 3′ fragments by magnetic bead precipitation.

Construct:

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: Three DGE libraries were constructed from total RNA from monoploid, diploid and triploid plants. .. Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end.

Article Title: Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis
Article Snippet: Four DGE libraries were constructed from the total RNA sample of the worms exposed to 50 μM sulfide for 0, 6, 24 or 48 h. For all libraries, mRNA was purified from 6 μg of total RNA sample using oligo(dT) magnetic beads and was reverse-transcribed into double-stranded cDNA (SuperScript II, Invitrogen). .. After removing the 3′ fragment via magnetic bead precipitation, Illumina adapter 2 was introduced at the site of MmeI cleavage, resulting in a library of tagged cDNA containing different adaptors on each end.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends. .. A 15 cycle PCR was performed with two primers (primer GX1 and primer GX2) that anneal to the ends of the adapters to enrich the adapter-ligated cDNA construct.

Adsorption:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: Six micrograms of total RNA were extracted and the mRNA was purified via Biotin-Oligo (dT) magnetic bead adsorption. .. The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library.

Article Title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani
Article Snippet: 20 μg of total RNA and 6 μg of mRNA were obtained and purified by adsorption of biotin Oligo(dT) magnetic beads. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Real-time Polymerase Chain Reaction:

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends. .. The amplified cDNA construct was purified from a 6% TBE PAGE gel.

Microarray:

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: Using the differential gene expression (DGE) method, which generates absolute rather than relative gene expression measurements and avoids many of the inherent limitations of microarray analyses, the gene expression variations were analyzed between the samples exposed to EMF and the control groups. .. After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library.

Incubation:

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: Total RNA from the three samples was incubated with magnetic oligo (dT) beads. .. Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end.

Formalin-fixed Paraffin-Embedded:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Total RNA was extracted from fixed cells using High pure FFPE RNA Micro Kit (Roche Diagnostic Corp., Indianapolis, IN) and submitted to BGI-Shenzhen (China) for lllumina sequencing. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Expressing:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: Paragraph title: Digital expression library construction and solexa sequencing ... The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library.

Article Title: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content
Article Snippet: Paragraph title: RNA sample preparation and digital gene expression ... Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed.

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: Using the differential gene expression (DGE) method, which generates absolute rather than relative gene expression measurements and avoids many of the inherent limitations of microarray analyses, the gene expression variations were analyzed between the samples exposed to EMF and the control groups. .. After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library.

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: Paragraph title: Digital gene expression profile (DGE) library construction and sequencing ... Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end.

Article Title: Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål
Article Snippet: Paragraph title: Analysis of JGM-induced gene expression profiles ... After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags.

Article Title: RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens
Article Snippet: Paragraph title: Analysis of JGM-induced gene expression profiles ... After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags.

Article Title: Whole Genome Wide Expression Profiles on Germination of Verticillium dahliae Microsclerotia
Article Snippet: Paragraph title: RNA extraction and digital expression library preparation ... After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′-end of the cDNA tag.

Article Title: Identification of drought stress-responsive transcription factors in ramie (Boehmeria nivea L. Gaud)
Article Snippet: Paragraph title: Preparation of digital expression libraries ... After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Article Title: Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent
Article Snippet: Paragraph title: Construction of the digital gene expression library, and Solexa sequencing ... After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments.

Article Title: Integrated analysis of miRNA and mRNA expression profiles in response to Cd exposure in rice seedlings
Article Snippet: Paragraph title: Differential gene expression (DGE) library construction and Illumina sequencing ... Illumina adapter 2 was introduced at the site of Mme I cleavage after removing the 3’ fragment via magnetic bead precipitation.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: Paragraph title: Digital Gene Expression (DGE) library preparation and sequencing ... The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends.

Article Title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani
Article Snippet: Paragraph title: Preparation of digital expression libraries ... After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Article Title: Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd)
Article Snippet: Paragraph title: Digital gene expression (DGE) library construction and sequencing ... The Illumina adapter 2 was then ligated to the 3′ ends of the tag.

Article Title: Whole genome wide expression profiles of Vitis amurensis grape responding to downy mildew by using Solexa sequencing technology
Article Snippet: Paragraph title: Preparation of Digital Expression Libraries ... After removing 3' fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3' end of the cDNA tag.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Paragraph title: DGE and differential expression analysis ... Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

RNA Binding Assay:

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: In brief, mRNA was isolated from 4 μg of total RNA by binding the mRNA to a magnetic Oligo dT beads. .. The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends.

Derivative Assay:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library. .. After double strand denaturation, the single chain molecules were fixed onto the Solexa Sequencing Chip (flow cell).

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: Subsequently, the cDNA samples were digested using the restriction enzyme Nla III, which recognizes and cuts the most 3′ “CATG.” Once digested into fragments, cDNA were ligated to Illumina adapter 1, which contains a recognition site for enzyme Mme I, and the enzyme Mme I was used to create the 17 bp tag. .. The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends. .. A 15 cycle PCR was performed with two primers (primer GX1 and primer GX2) that anneal to the ends of the adapters to enrich the adapter-ligated cDNA construct.

Flow Cytometry:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library. .. The resulting 85 base fragments were purified by 6% TBE PAGE Gel electrophoresis.

Article Title: Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent
Article Snippet: After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments. .. The resultant 85 base strips were purified by 6% TBE-PAGE gel electrophoresis.

Article Title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani
Article Snippet: After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Ligation:

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Generated:

Article Title: Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis
Article Snippet: The junction of Illumina adapter 1 and CATG generated the recognition site for MmeI. .. After removing the 3′ fragment via magnetic bead precipitation, Illumina adapter 2 was introduced at the site of MmeI cleavage, resulting in a library of tagged cDNA containing different adaptors on each end.

Article Title: DeepSAGE Based Differential Gene Expression Analysis under Cold and Freeze Stress in Seabuckthorn (Hippophae rhamnoides L.)
Article Snippet: The 5’ ends of the tags were generated by digestion of bead bound cDNA with restriction enzyme Nla III having CATG recognition site. .. Illumina adapter 2 was ligated to the 3’ ends of the tags to generate a tag library by acquiring different adaptors at both ends.

Polymerase Chain Reaction:

Article Title: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content
Article Snippet: Adapter 1 (Illumina) was then ligated to the 5′-end, and the restriction enzyme Mme I was then added, which can recognize the nucleotide sequences composed of adapter 1 and CATG, and then cut at the downstream 17 bp site, to produce tags labelled with adapter 1. .. Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed. .. PCR products were run on 6% TBE PAGE and purified, which were then submitted to Illumina HiSeq™ 2000 for digital gene expression analysis (BGI, Shengzhen).

Article Title: Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent
Article Snippet: After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments. .. After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Binding Assay:

Article Title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani
Article Snippet: After mRNA’s binding, cDNA synthesis was performed. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Nucleic Acid Electrophoresis:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library. .. The cDNA fragments with GEX adapters 1 and 2 were subject to 15 cycles of linear PCR amplification by Phusion polymerase (Finnzymes, Espoo, Finland).

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: The quality and quantity of RNA were examined by gel electrophoresis and spectrophotometry. .. Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end.

Article Title: Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent
Article Snippet: After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments. .. A linear PCR amplification with 15 cycles was performed with primers complementary to the adapter sequences, to enrich the samples with the desired fragments, using Phusion polymerase (Finnzymes, Espoo, Finland).

Fluorescence:

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library. .. Each molecule was grown into a single-molecule cluster sequencing template through in situ amplification.

Magnetic Beads:

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: The cDNA fragments with 3' ends were then purified with magnetic beads, and Illumina adapter 1 was added to their 5' ends. .. After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library.

Article Title: Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål
Article Snippet: MmeI was used to cut the cDNA fragments 17 bp downstream of the CATG site. .. After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags. .. After fifteen cycles of linear PCR amplification, 105 bp fragments were purified on 6% TBE PAGE.

Article Title: RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens
Article Snippet: MmeI was used to cut the cDNA fragments at 17 bp downstream of the CATG site. .. After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags. .. After fifteen cycles of linear PCR amplification, 105-bp fragments were purified on 6% TBE PAGE.

Article Title: Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis
Article Snippet: Four DGE libraries were constructed from the total RNA sample of the worms exposed to 50 μM sulfide for 0, 6, 24 or 48 h. For all libraries, mRNA was purified from 6 μg of total RNA sample using oligo(dT) magnetic beads and was reverse-transcribed into double-stranded cDNA (SuperScript II, Invitrogen). .. After removing the 3′ fragment via magnetic bead precipitation, Illumina adapter 2 was introduced at the site of MmeI cleavage, resulting in a library of tagged cDNA containing different adaptors on each end.

Article Title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani
Article Snippet: 20 μg of total RNA and 6 μg of mRNA were obtained and purified by adsorption of biotin Oligo(dT) magnetic beads. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Article Title: Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd)
Article Snippet: All cDNA fragments with 3′ ends were purified with magnetic beads before their 5′ ends were ligated to Illumina adapter 1. .. The Illumina adapter 2 was then ligated to the 3′ ends of the tag.

Article Title: Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Article Snippet: First, poly(A)-containing mRNA molecules were purified from total RNA using poly(T) oligo-attached magnetic beads. .. Illumina adapter 2 was ligated to the 3′-end of the cDNA tag after removing the 3′ fragments by magnetic bead precipitation.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Briefly, mRNA was first isolated from the total RNA by using Oligo(dT) magnetic beads and then applied as template for cDNA synthesis. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Isolation:

Article Title: Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål
Article Snippet: RNA quality was evaluated using a 2100 Bioanalyzer (Agilent) according to the Illumina instructions. mRNA was isolated from 6 μg of total RNA from each sample using oligo (dT) magnetic beads, and then double-stranded cDNA was synthesized after reverse transcription using oligo (dT) primers bound to magnetic beads. .. After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags.

Article Title: RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens
Article Snippet: For BPH, mRNA was isolated form 6 μg of total RNA from each sample using oligo (dT) magnetic beads, and then double-stranded cDNA was synthesized after reverse transcription using oligo (dT) primer bound to magnetic beads. .. After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: In brief, mRNA was isolated from 4 μg of total RNA by binding the mRNA to a magnetic Oligo dT beads. .. The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Briefly, mRNA was first isolated from the total RNA by using Oligo(dT) magnetic beads and then applied as template for cDNA synthesis. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Labeling:

Article Title: DeepSAGE Based Differential Gene Expression Analysis under Cold and Freeze Stress in Seabuckthorn (Hippophae rhamnoides L.)
Article Snippet: Illumina adapter 2 was ligated to the 3’ ends of the tags to generate a tag library by acquiring different adaptors at both ends. .. Each molecule grows into a single-molecule cluster-sequencing template through an in situ amplification process.

Article Title: Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Article Snippet: Illumina adapter 2 was ligated to the 3′-end of the cDNA tag after removing the 3′ fragments by magnetic bead precipitation. .. Fragments were then digested and the single-chain molecules were fixed onto the Solexa Sequencing Chip.

Purification:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: These cDNA fragments were purified with magnetic bead precipitation and Illumina adapter 1(GEX adapter 1) was added to newly formed 5′ sticky end of cDNA fragments. .. The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library.

Article Title: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content
Article Snippet: Messenger RNAs were purified from 6 μg total RNA, and reversely transcribed into cDNA, which was then digested using the restriction enzyme Nla III (recognition sites: 5′-…CATG/GTAC…-3′). .. Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed.

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: The cDNA fragments with 3' ends were then purified with magnetic beads, and Illumina adapter 1 was added to their 5' ends. .. After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library.

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end. .. PCR amplification with 15 cycles was performed using primers complementary to the adapter sequences.

Article Title: Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis
Article Snippet: Four DGE libraries were constructed from the total RNA sample of the worms exposed to 50 μM sulfide for 0, 6, 24 or 48 h. For all libraries, mRNA was purified from 6 μg of total RNA sample using oligo(dT) magnetic beads and was reverse-transcribed into double-stranded cDNA (SuperScript II, Invitrogen). .. After removing the 3′ fragment via magnetic bead precipitation, Illumina adapter 2 was introduced at the site of MmeI cleavage, resulting in a library of tagged cDNA containing different adaptors on each end.

Article Title: Whole Genome Wide Expression Profiles on Germination of Verticillium dahliae Microsclerotia
Article Snippet: Double stranded cDNA was digested with the Nla III endonuclease to produce a bead-bound cDNA fragment containing sequence from the 3′-most CATG to the poly (A)-tail, and these 3′ cDNA fragments were purified using magnetic bead precipitation. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′-end of the cDNA tag.

Article Title: Identification of drought stress-responsive transcription factors in ramie (Boehmeria nivea L. Gaud)
Article Snippet: These 3′ cDNA fragments were purified by using magnetic bead precipitation and the Illumina adapter 1 (GEX adapter 1) was added to new 5′ end. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Article Title: Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent
Article Snippet: These 3′ cDNA fragments were purified using magnetic bead precipitation, and Illumina adapter 1 (GEX adapter 1) was added to the newly formed 5′ sticky end of the cDNA fragments. .. After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends. .. A 15 cycle PCR was performed with two primers (primer GX1 and primer GX2) that anneal to the ends of the adapters to enrich the adapter-ligated cDNA construct.

Article Title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani
Article Snippet: These 3′ cDNA fragments were purified using magnetic bead precipitation and the Illumina adapter 1 (GEX adapter 1) was added to new 5′ end. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Article Title: Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd)
Article Snippet: All cDNA fragments with 3′ ends were purified with magnetic beads before their 5′ ends were ligated to Illumina adapter 1. .. The Illumina adapter 2 was then ligated to the 3′ ends of the tag.

Article Title: Whole genome wide expression profiles of Vitis amurensis grape responding to downy mildew by using Solexa sequencing technology
Article Snippet: These 3' cDNA fragments were purified using magnetic bead precipitation and the Illumina adapter 1 (GEX adapter 1) was added to new 5' end. .. After removing 3' fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3' end of the cDNA tag.

Article Title: Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Article Snippet: While on the beads, double-stranded cDNA was digested with NlaIII endonuclease to produce a bead-bound cDNA fragment containing the sequence from the 3′-most CATG to the poly(A)-tail. cDNA fragments with 3′-ends were purified by magnetic bead precipitation, and Illumina adapter 1 was added to the 5′-ends. .. Illumina adapter 2 was ligated to the 3′-end of the cDNA tag after removing the 3′ fragments by magnetic bead precipitation.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: The resultant cDNA was endonuclease digested ( Nla III), which recognizes and cuts the most 3′ ‘CATG’, and ligated to Illumina adapter 1. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system. .. Raw image data were transformed by base calling into raw sequence data and then transformed into clean tags for alignment to the human reference genome hg19.

Sequencing:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: Paragraph title: Digital expression library construction and solexa sequencing ... The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library.

Article Title: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content
Article Snippet: Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed. .. Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed.

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: Paragraph title: DEG library preparation and sequencing ... After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library.

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: Paragraph title: Digital gene expression profile (DGE) library construction and sequencing ... Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end.

Article Title: Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål
Article Snippet: After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags. .. After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags.

Article Title: RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens
Article Snippet: After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags. .. After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags.

Article Title: Transcriptional response to sulfide in the Echiuran Worm Urechis unicinctus by digital gene expression analysis
Article Snippet: Paragraph title: DGE library construction, sequencing and annotation ... After removing the 3′ fragment via magnetic bead precipitation, Illumina adapter 2 was introduced at the site of MmeI cleavage, resulting in a library of tagged cDNA containing different adaptors on each end.

Article Title: Whole Genome Wide Expression Profiles on Germination of Verticillium dahliae Microsclerotia
Article Snippet: Mme I cuts 17 bp downstream of the CATG site, producing 17 bp cDNA sequence tags with the adapter 1. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′-end of the cDNA tag.

Article Title: Identification of drought stress-responsive transcription factors in ramie (Boehmeria nivea L. Gaud)
Article Snippet: The enzyme cuts 17 bp downstream of the CATG site, producing 17 bp cDNA sequence tags with adapter 1. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Article Title: Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent
Article Snippet: Paragraph title: Construction of the digital gene expression library, and Solexa sequencing ... After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments.

Article Title: Integrated analysis of miRNA and mRNA expression profiles in response to Cd exposure in rice seedlings
Article Snippet: Paragraph title: Differential gene expression (DGE) library construction and Illumina sequencing ... Illumina adapter 2 was introduced at the site of Mme I cleavage after removing the 3’ fragment via magnetic bead precipitation.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: Paragraph title: Digital Gene Expression (DGE) library preparation and sequencing ... The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends.

Article Title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani
Article Snippet: After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Article Title: DeepSAGE Based Differential Gene Expression Analysis under Cold and Freeze Stress in Seabuckthorn (Hippophae rhamnoides L.)
Article Snippet: Paragraph title: Tag Preparation and Illumina Sequencing ... Illumina adapter 2 was ligated to the 3’ ends of the tags to generate a tag library by acquiring different adaptors at both ends.

Article Title: Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd)
Article Snippet: Paragraph title: Digital gene expression (DGE) library construction and sequencing ... The Illumina adapter 2 was then ligated to the 3′ ends of the tag.

Article Title: Whole genome wide expression profiles of Vitis amurensis grape responding to downy mildew by using Solexa sequencing technology
Article Snippet: The enzyme cuts 17 bp downstream of the CATG site, producing 17 bp cDNA sequence tags with adapter 1. .. After removing 3' fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3' end of the cDNA tag.

Article Title: Analysis of the Transcriptional Differences between Indigenous and Invasive Whiteflies Reveals Possible Mechanisms of Whitefly Invasion
Article Snippet: Paragraph title: DGE Library Preparation and Sequencing ... After removing 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at 3′ ends of tags, acquiring 21 bp tags with different adapters at both ends to form a tag library.

Article Title: Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Article Snippet: Paragraph title: DGE sequencing and tag annotation ... Illumina adapter 2 was ligated to the 3′-end of the cDNA tag after removing the 3′ fragments by magnetic bead precipitation.

Article Title: Histone methylation mediates plasticity of human FOXP3+ regulatory T cells by modulating signature gene expressions
Article Snippet: Total RNA was extracted from fixed cells using High pure FFPE RNA Micro Kit (Roche Diagnostic Corp., Indianapolis, IN) and submitted to BGI-Shenzhen (China) for lllumina sequencing. .. Following a sequential digestion of the 5′ end with Mme I and ligation to Illumina adapter 2, the DNA fragments (library) were amplified by PCR, gel purified and sequenced on the HiSeq™ 2000 system.

Polyacrylamide Gel Electrophoresis:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library. .. The cDNA fragments with GEX adapters 1 and 2 were subject to 15 cycles of linear PCR amplification by Phusion polymerase (Finnzymes, Espoo, Finland).

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end. .. PCR amplification with 15 cycles was performed using primers complementary to the adapter sequences.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends. .. A 15 cycle PCR was performed with two primers (primer GX1 and primer GX2) that anneal to the ends of the adapters to enrich the adapter-ligated cDNA construct.

Article Title: Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Article Snippet: Illumina adapter 2 was ligated to the 3′-end of the cDNA tag after removing the 3′ fragments by magnetic bead precipitation. .. These adapter-ligated cDNA tags were enriched using PCR primers that anneal to the adaptor ends.

Chromatin Immunoprecipitation:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library. .. The resulting 85 base fragments were purified by 6% TBE PAGE Gel electrophoresis.

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library. .. After 15 cycles of linear PCR amplification, 85-base strips were purified by 6% Tris-borate-EDTA (TBE)-PAGE gel electrophoresis.

Article Title: Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål
Article Snippet: After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags. .. After fifteen cycles of linear PCR amplification, 105 bp fragments were purified on 6% TBE PAGE.

Article Title: RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens
Article Snippet: After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags. .. After fifteen cycles of linear PCR amplification, 105-bp fragments were purified on 6% TBE PAGE.

Article Title: Genome-Wide Transcriptional Profiles of the Berry Skin of Two Red Grape Cultivars (Vitis vinifera) in Which Anthocyanin Synthesis Is Sunlight-Dependent or -Independent
Article Snippet: After removing the 3′ fragments by magnetic bead precipitation, Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragments. .. The resultant 85 base strips were purified by 6% TBE-PAGE gel electrophoresis.

Article Title: Genome expression profile analysis of the maize sheath in response to inoculation to R. solani
Article Snippet: After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag. .. After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′ end of the cDNA tag.

Article Title: DeepSAGE Based Differential Gene Expression Analysis under Cold and Freeze Stress in Seabuckthorn (Hippophae rhamnoides L.)
Article Snippet: Illumina adapter 2 was ligated to the 3’ ends of the tags to generate a tag library by acquiring different adaptors at both ends. .. After 15 cycles of linear PCR amplification, 105 bp fragments were purified by 6% TBE—PAGE (Tris/Borate/EDTA—Poly Acrylamide Gel Electrophoresis).

Article Title: Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd)
Article Snippet: The Illumina adapter 2 was then ligated to the 3′ ends of the tag. .. The library was amplified by PCR for 15 cycles and 90 bp strips were purified by PAGE gel electrophoresis.

Article Title: Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Article Snippet: Illumina adapter 2 was ligated to the 3′-end of the cDNA tag after removing the 3′ fragments by magnetic bead precipitation. .. The resulting 85-bp fragments were purified by 6% TBE PAGE.

RNA Extraction:

Article Title: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content
Article Snippet: Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed. .. Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed.

Article Title: Whole Genome Wide Expression Profiles on Germination of Verticillium dahliae Microsclerotia
Article Snippet: Paragraph title: RNA extraction and digital expression library preparation ... After removing 3′ fragments with magnetic bead precipitation, the Illumina adapter 2 (GEX adapter 2) was ligated to 3′-end of the cDNA tag.

Sample Prep:

Article Title: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content
Article Snippet: Paragraph title: RNA sample preparation and digital gene expression ... Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed.

Article Title: Integrated analysis of miRNA and mRNA expression profiles in response to Cd exposure in rice seedlings
Article Snippet: The DGE libraries for five samples were processed in parallel using Illumina sample preparation kits. .. Illumina adapter 2 was introduced at the site of Mme I cleavage after removing the 3’ fragment via magnetic bead precipitation.

Article Title: Dynamic gene expression profiles during postnatal development of porcine subcutaneous adipose
Article Snippet: The DGE libraries preparation for HLB was carried out using Illumina Gene Expression Sample Prep Kit according to the manufacturer’s protocol. .. The Illumina adapter 2 was ligated after Mme I digestion and obtains 21 bp tag derived from a single transcript with Illumina adapters attached to both ends.

Article Title: Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd)
Article Snippet: The tag library was prepared using the Illumina gene expression sample prep kit. .. The Illumina adapter 2 was then ligated to the 3′ ends of the tag.

In Situ:

Article Title: Genome-Wide Transcriptional Profile Analysis of Prunus persica in Response to Low Sink Demand after Fruit Removal
Article Snippet: The 3′ fragments were removed using magnetic bead precipitation; and the Illumina adapter 2 (GEX adapter 2) was ligated to the new 3′ end of the cDNA fragment, which represented the tag library. .. After double strand denaturation, the single chain molecules were fixed onto the Solexa Sequencing Chip (flow cell).

Article Title: Exposure of ELF-EMF and RF-EMF Increase the Rate of Glucose Transport and TCA Cycle in Budding Yeast
Article Snippet: After removing the 3′ fragments with magnetic bead precipitation, Illumina adapter 2 was introduced at the 3′ ends of the tags, resulting in the acquisition of tags with different adapters at each end to form a tag library. .. These strips were then digested, and the single-chain molecules were fixed on an Illumina chip for sequencing.

Article Title: Adipose triglyceride lipase (Atgl) mediates the antibiotic jinggangmycin-stimulated reproduction in the brown planthopper, Nilaparvata lugens Stål
Article Snippet: After collecting the cDNA fraction with magnetic beads, Illumina adapter 2 was ligated at the 3′ ends of the tags. .. After denaturation, the single-strand sequences were fixed onto an Illumina HiSeq TM 2000 chip.

Article Title: RNAi knockdown of acetyl-CoA carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, Nilaparvata lugens
Article Snippet: After removing the cDNA fraction with magnetic beads, the Illumina adapter 2 was ligated at the 3′ end of the tags. .. After denaturation, the single-stranded sequences were fixed onto an Illumina HiSeq TM 2000 chip.

Article Title: DeepSAGE Based Differential Gene Expression Analysis under Cold and Freeze Stress in Seabuckthorn (Hippophae rhamnoides L.)
Article Snippet: Illumina adapter 2 was ligated to the 3’ ends of the tags to generate a tag library by acquiring different adaptors at both ends. .. After denaturation, the single chain molecules were fixed onto the Illumina Sequencing Chip (flowcell).

Spectrophotometry:

Article Title: Global Methylation Patterns and Their Relationship with Gene Expression and Small RNA in Rice Lines with Different Ploidy
Article Snippet: The quality and quantity of RNA were examined by gel electrophoresis and spectrophotometry. .. Following digestion with MmeI the adapter 1 ligated tags were detached from the beads and the Illumina adapter 2 was ligated to the 3′ end.

Produced:

Article Title: Transcriptome profiling of fruit development and maturation in Chinese white pear (Pyrus bretschneideri Rehd)
Article Snippet: A 21 bp tag with the adaptor 1 was produced after digested with MmeI (an enzyme that recognizes the junction of the Illumina adaptor 1 and the CATG site). .. The Illumina adapter 2 was then ligated to the 3′ ends of the tag.

Concentration Assay:

Article Title: Embryonic transcriptome and proteome analyses on hepatic lipid metabolism in chickens divergently selected for abdominal fat content
Article Snippet: Total RNAs from the 10 samples were extracted using Trizol (Invitrogen), and RNA quality and concentration were evaluated, to ensure RNA integrity number (RIN) ≥ 9. .. Then adapter 2 (Illumina) was ligated with these tags, and 15 cycles of PCR were performed.

High Throughput Screening Assay:

Article Title: Transcriptome Profiling Analysis Reveals That Flavonoid and Ascorbate-Glutathione Cycle Are Important during Anther Development in Upland Cotton
Article Snippet: At least 6 µg of total RNA ( > 300 ng·µL−1 ) from each sample of CCRI 040029 was sent to the “Beijing Genomics Institute” (BGI, Shenzhen, China) for high-throughput Solexa sequencing. .. Illumina adapter 2 was ligated to the 3′-end of the cDNA tag after removing the 3′ fragments by magnetic bead precipitation.

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  • 79
    Illumina Inc adapter ad 2
    Schematic of DR-Seq for sequencing gDNA and mRNA from the same cell. (a) gDNA and mRNA/cDNA are shown in red and green, respectively. Following single-cell lysis and RT using adapter Ad-1x (purple), gDNA and single stranded cDNA are amplified by <t>Ad-2</t> (blue) using a quasilinear amplification strategy. The majority of the short amplicons contain Ad-2 at both ends and cDNA-derived amplicons contain Ad-2 at one end and Ad-1x at the other end. The sample is then split into two halves and processed separately to amplify and sequence gDNA or cDNA. (b) Distribution of reads within 100 nucleotides of the gene Dppa5a for two single cells (red and black) as a function of the random priming location by adapter Ad-2. The unique length-based identifiers found in the two cells can be used to count the original number of cDNA molecules within each cell and minimize amplification biases. The figure shows that distinct positions are randomly primed within each cell with high affinity binding sites being preferentially primed. The size of the dots indicate the binding propensity of each location. For most genes such as Dppa5a , the number of theoretical binding sites far exceed the number of length-based identifiers detected, thereby enabling length-based identifiers to accurately estimate the original number of cDNA molecules.
    Adapter Ad 2, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/adapter ad 2/product/Illumina Inc
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    adapter ad 2 - by Bioz Stars, 2019-10
    79/100 stars
      Buy from Supplier

    76
    Illumina Inc truseq3 pe 2 adapter sequences
    Schematic of DR-Seq for sequencing gDNA and mRNA from the same cell. (a) gDNA and mRNA/cDNA are shown in red and green, respectively. Following single-cell lysis and RT using adapter Ad-1x (purple), gDNA and single stranded cDNA are amplified by <t>Ad-2</t> (blue) using a quasilinear amplification strategy. The majority of the short amplicons contain Ad-2 at both ends and cDNA-derived amplicons contain Ad-2 at one end and Ad-1x at the other end. The sample is then split into two halves and processed separately to amplify and sequence gDNA or cDNA. (b) Distribution of reads within 100 nucleotides of the gene Dppa5a for two single cells (red and black) as a function of the random priming location by adapter Ad-2. The unique length-based identifiers found in the two cells can be used to count the original number of cDNA molecules within each cell and minimize amplification biases. The figure shows that distinct positions are randomly primed within each cell with high affinity binding sites being preferentially primed. The size of the dots indicate the binding propensity of each location. For most genes such as Dppa5a , the number of theoretical binding sites far exceed the number of length-based identifiers detected, thereby enabling length-based identifiers to accurately estimate the original number of cDNA molecules.
    Truseq3 Pe 2 Adapter Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 76/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/truseq3 pe 2 adapter sequences/product/Illumina Inc
    Average 76 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    truseq3 pe 2 adapter sequences - by Bioz Stars, 2019-10
    76/100 stars
      Buy from Supplier

    91
    Illumina Inc partial illumina adaptor sequences
    Graphic representation of library preparation. Ig-seq library preparation consists of 4 steps: (1) mRNA purification and reverse transcription, (2) IGH-specific double strand cDNA synthesis with multiplex primers, both while bound to poly-T magnetic beads, () two step semi-nested PCR, and (1) <t>Illumina</t> indexing.
    Partial Illumina Adaptor Sequences, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/partial illumina adaptor sequences/product/Illumina Inc
    Average 91 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    partial illumina adaptor sequences - by Bioz Stars, 2019-10
    91/100 stars
      Buy from Supplier

    Image Search Results


    Schematic of DR-Seq for sequencing gDNA and mRNA from the same cell. (a) gDNA and mRNA/cDNA are shown in red and green, respectively. Following single-cell lysis and RT using adapter Ad-1x (purple), gDNA and single stranded cDNA are amplified by Ad-2 (blue) using a quasilinear amplification strategy. The majority of the short amplicons contain Ad-2 at both ends and cDNA-derived amplicons contain Ad-2 at one end and Ad-1x at the other end. The sample is then split into two halves and processed separately to amplify and sequence gDNA or cDNA. (b) Distribution of reads within 100 nucleotides of the gene Dppa5a for two single cells (red and black) as a function of the random priming location by adapter Ad-2. The unique length-based identifiers found in the two cells can be used to count the original number of cDNA molecules within each cell and minimize amplification biases. The figure shows that distinct positions are randomly primed within each cell with high affinity binding sites being preferentially primed. The size of the dots indicate the binding propensity of each location. For most genes such as Dppa5a , the number of theoretical binding sites far exceed the number of length-based identifiers detected, thereby enabling length-based identifiers to accurately estimate the original number of cDNA molecules.

    Journal: Nature biotechnology

    Article Title: Integrated genome and transcriptome sequencing from the same cell

    doi: 10.1038/nbt.3129

    Figure Lengend Snippet: Schematic of DR-Seq for sequencing gDNA and mRNA from the same cell. (a) gDNA and mRNA/cDNA are shown in red and green, respectively. Following single-cell lysis and RT using adapter Ad-1x (purple), gDNA and single stranded cDNA are amplified by Ad-2 (blue) using a quasilinear amplification strategy. The majority of the short amplicons contain Ad-2 at both ends and cDNA-derived amplicons contain Ad-2 at one end and Ad-1x at the other end. The sample is then split into two halves and processed separately to amplify and sequence gDNA or cDNA. (b) Distribution of reads within 100 nucleotides of the gene Dppa5a for two single cells (red and black) as a function of the random priming location by adapter Ad-2. The unique length-based identifiers found in the two cells can be used to count the original number of cDNA molecules within each cell and minimize amplification biases. The figure shows that distinct positions are randomly primed within each cell with high affinity binding sites being preferentially primed. The size of the dots indicate the binding propensity of each location. For most genes such as Dppa5a , the number of theoretical binding sites far exceed the number of length-based identifiers detected, thereby enabling length-based identifiers to accurately estimate the original number of cDNA molecules.

    Article Snippet: Next, to remove adapter Ad-2 from the PCR product prior to preparing Illumina libraries, another PCR was done starting with 80 ng of product from the previous step.

    Techniques: Sequencing, Lysis, Amplification, Derivative Assay, Binding Assay

    Graphic representation of library preparation. Ig-seq library preparation consists of 4 steps: (1) mRNA purification and reverse transcription, (2) IGH-specific double strand cDNA synthesis with multiplex primers, both while bound to poly-T magnetic beads, () two step semi-nested PCR, and (1) Illumina indexing.

    Journal: Frontiers in Immunology

    Article Title: Novel Method for High-Throughput Full-Length IGHV-D-J Sequencing of the Immune Repertoire from Bulk B-Cells with Single-Cell Resolution

    doi: 10.3389/fimmu.2017.01157

    Figure Lengend Snippet: Graphic representation of library preparation. Ig-seq library preparation consists of 4 steps: (1) mRNA purification and reverse transcription, (2) IGH-specific double strand cDNA synthesis with multiplex primers, both while bound to poly-T magnetic beads, () two step semi-nested PCR, and (1) Illumina indexing.

    Article Snippet: After RNase H treatment, second-strand synthesis was performed in solid phase in 10 µl using Q5 Polymerase (NEB) and a mix of 13 primers covering all IGHV leader sequence segments reported in the IMGT database with a maximum of one mismatch, containing 13 to 16 random nt and partial Illumina adaptor sequences (37°C 20 min, 98°C 30 s, 62°C 2 min, and 72°C 10 min).

    Techniques: Purification, Multiplex Assay, Magnetic Beads, Nested PCR