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ad169 hcmv strain  (ATCC)


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    ATCC ad169 hcmv strain
    Ad169 Hcmv Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ad169 hcmv strain/product/ATCC
    Average 94 stars, based on 1 article reviews
    ad169 hcmv strain - by Bioz Stars, 2025-03
    94/100 stars

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    Comparison of HCMV <t>(AD169)</t> infection between NHDFCs and hiPSCs. (A–C) NHDFCs. (A) Control (uninfected). (B) 3 days post-HCMV infection at an MOI of 10. (C) Seven days post-HCMV infection at an MOI of 10. Arrows indicate cytopathic changes. (D–F) hiPSCs. (C) Control (uninfected). (D) Three days post-HCMV infection at an MOI of 10. (E) Seven days post-HCMV infection at an MOI of 10. Black lines represent the border of the hiPSC colonies. Scale bar: 100 µm. (G–K) HCMV pp65 expression in NHDFCs and hiPSCs 12 h post-infection. (G and I) HCMV pp65 antigen-positive (green) NHDFCs (G) and hiPSCs (I). (H and J) DAPI staining (blue) of NHDFCs (H) and hiPSCs (J). Scale bar: 50 µm. (K) Comparison of the HCMV pp65-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (L–P) HCMV IE1/2 + pUL44 expression in NHDFCs and iPSCs 12 h post-infection. (L and N) HCMV IE1/2 + pUL44 antigen-positive (green) NHDFCs (L) and hiPSCs (N). (M and O) DAPI staining (blue) of NHDFCs (M) and hiPSCs (O). Scale bar: 40 µm. (K) Comparison of the HCMV IE1/2 + pUL44-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (Q–S) NHDFCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (Q), Immunofluorescence images of pp65 (R) and DAPI (S). (T–V) hiPSCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (T), Immunofluorescence images of pp65 (U) and DAPI (V). Scale bar: 20 µm. HCMV, human cytomegalovirus; hiPSC, human induced pluripotent stem cell; IE1/2, immediate-early 1/2; MOI, multiplicity of infection; NHDFC, neonatal human dermal fibroblast cell.
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    Comparison of HCMV <t>(AD169)</t> infection between NHDFCs and hiPSCs. (A–C) NHDFCs. (A) Control (uninfected). (B) 3 days post-HCMV infection at an MOI of 10. (C) Seven days post-HCMV infection at an MOI of 10. Arrows indicate cytopathic changes. (D–F) hiPSCs. (C) Control (uninfected). (D) Three days post-HCMV infection at an MOI of 10. (E) Seven days post-HCMV infection at an MOI of 10. Black lines represent the border of the hiPSC colonies. Scale bar: 100 µm. (G–K) HCMV pp65 expression in NHDFCs and hiPSCs 12 h post-infection. (G and I) HCMV pp65 antigen-positive (green) NHDFCs (G) and hiPSCs (I). (H and J) DAPI staining (blue) of NHDFCs (H) and hiPSCs (J). Scale bar: 50 µm. (K) Comparison of the HCMV pp65-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (L–P) HCMV IE1/2 + pUL44 expression in NHDFCs and iPSCs 12 h post-infection. (L and N) HCMV IE1/2 + pUL44 antigen-positive (green) NHDFCs (L) and hiPSCs (N). (M and O) DAPI staining (blue) of NHDFCs (M) and hiPSCs (O). Scale bar: 40 µm. (K) Comparison of the HCMV IE1/2 + pUL44-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (Q–S) NHDFCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (Q), Immunofluorescence images of pp65 (R) and DAPI (S). (T–V) hiPSCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (T), Immunofluorescence images of pp65 (U) and DAPI (V). Scale bar: 20 µm. HCMV, human cytomegalovirus; hiPSC, human induced pluripotent stem cell; IE1/2, immediate-early 1/2; MOI, multiplicity of infection; NHDFC, neonatal human dermal fibroblast cell.
    Hcmv Strain Ad169, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Comparison of HCMV <t>(AD169)</t> infection between NHDFCs and hiPSCs. (A–C) NHDFCs. (A) Control (uninfected). (B) 3 days post-HCMV infection at an MOI of 10. (C) Seven days post-HCMV infection at an MOI of 10. Arrows indicate cytopathic changes. (D–F) hiPSCs. (C) Control (uninfected). (D) Three days post-HCMV infection at an MOI of 10. (E) Seven days post-HCMV infection at an MOI of 10. Black lines represent the border of the hiPSC colonies. Scale bar: 100 µm. (G–K) HCMV pp65 expression in NHDFCs and hiPSCs 12 h post-infection. (G and I) HCMV pp65 antigen-positive (green) NHDFCs (G) and hiPSCs (I). (H and J) DAPI staining (blue) of NHDFCs (H) and hiPSCs (J). Scale bar: 50 µm. (K) Comparison of the HCMV pp65-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (L–P) HCMV IE1/2 + pUL44 expression in NHDFCs and iPSCs 12 h post-infection. (L and N) HCMV IE1/2 + pUL44 antigen-positive (green) NHDFCs (L) and hiPSCs (N). (M and O) DAPI staining (blue) of NHDFCs (M) and hiPSCs (O). Scale bar: 40 µm. (K) Comparison of the HCMV IE1/2 + pUL44-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (Q–S) NHDFCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (Q), Immunofluorescence images of pp65 (R) and DAPI (S). (T–V) hiPSCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (T), Immunofluorescence images of pp65 (U) and DAPI (V). Scale bar: 20 µm. HCMV, human cytomegalovirus; hiPSC, human induced pluripotent stem cell; IE1/2, immediate-early 1/2; MOI, multiplicity of infection; NHDFC, neonatal human dermal fibroblast cell.
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    Image Search Results


    Comparison of HCMV (AD169) infection between NHDFCs and hiPSCs. (A–C) NHDFCs. (A) Control (uninfected). (B) 3 days post-HCMV infection at an MOI of 10. (C) Seven days post-HCMV infection at an MOI of 10. Arrows indicate cytopathic changes. (D–F) hiPSCs. (C) Control (uninfected). (D) Three days post-HCMV infection at an MOI of 10. (E) Seven days post-HCMV infection at an MOI of 10. Black lines represent the border of the hiPSC colonies. Scale bar: 100 µm. (G–K) HCMV pp65 expression in NHDFCs and hiPSCs 12 h post-infection. (G and I) HCMV pp65 antigen-positive (green) NHDFCs (G) and hiPSCs (I). (H and J) DAPI staining (blue) of NHDFCs (H) and hiPSCs (J). Scale bar: 50 µm. (K) Comparison of the HCMV pp65-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (L–P) HCMV IE1/2 + pUL44 expression in NHDFCs and iPSCs 12 h post-infection. (L and N) HCMV IE1/2 + pUL44 antigen-positive (green) NHDFCs (L) and hiPSCs (N). (M and O) DAPI staining (blue) of NHDFCs (M) and hiPSCs (O). Scale bar: 40 µm. (K) Comparison of the HCMV IE1/2 + pUL44-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (Q–S) NHDFCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (Q), Immunofluorescence images of pp65 (R) and DAPI (S). (T–V) hiPSCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (T), Immunofluorescence images of pp65 (U) and DAPI (V). Scale bar: 20 µm. HCMV, human cytomegalovirus; hiPSC, human induced pluripotent stem cell; IE1/2, immediate-early 1/2; MOI, multiplicity of infection; NHDFC, neonatal human dermal fibroblast cell.

    Journal: Journal of Virology

    Article Title: Human induced pluripotent stem cells are resistant to human cytomegalovirus infection primarily at the attachment level due to the reduced expression of cell-surface heparan sulfate

    doi: 10.1128/jvi.01278-23

    Figure Lengend Snippet: Comparison of HCMV (AD169) infection between NHDFCs and hiPSCs. (A–C) NHDFCs. (A) Control (uninfected). (B) 3 days post-HCMV infection at an MOI of 10. (C) Seven days post-HCMV infection at an MOI of 10. Arrows indicate cytopathic changes. (D–F) hiPSCs. (C) Control (uninfected). (D) Three days post-HCMV infection at an MOI of 10. (E) Seven days post-HCMV infection at an MOI of 10. Black lines represent the border of the hiPSC colonies. Scale bar: 100 µm. (G–K) HCMV pp65 expression in NHDFCs and hiPSCs 12 h post-infection. (G and I) HCMV pp65 antigen-positive (green) NHDFCs (G) and hiPSCs (I). (H and J) DAPI staining (blue) of NHDFCs (H) and hiPSCs (J). Scale bar: 50 µm. (K) Comparison of the HCMV pp65-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (L–P) HCMV IE1/2 + pUL44 expression in NHDFCs and iPSCs 12 h post-infection. (L and N) HCMV IE1/2 + pUL44 antigen-positive (green) NHDFCs (L) and hiPSCs (N). (M and O) DAPI staining (blue) of NHDFCs (M) and hiPSCs (O). Scale bar: 40 µm. (K) Comparison of the HCMV IE1/2 + pUL44-positive cell ratio between NHDFCs and hiPSCs. ★★P < 0.01. (Q–S) NHDFCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (Q), Immunofluorescence images of pp65 (R) and DAPI (S). (T–V) hiPSCs after HCMV infection at an MOI of 10, 12 h post-infection (high magnification). Phase contrast image (T), Immunofluorescence images of pp65 (U) and DAPI (V). Scale bar: 20 µm. HCMV, human cytomegalovirus; hiPSC, human induced pluripotent stem cell; IE1/2, immediate-early 1/2; MOI, multiplicity of infection; NHDFC, neonatal human dermal fibroblast cell.

    Article Snippet: The HCMV strain AD169, provided by the Department of Regenerative and Infectious pathology, Hamamatsu University School of Medicine, was cultivated in human embryonic lung fibroblasts using an MOI of 0.01.

    Techniques: Comparison, Infection, Expressing, Staining, Immunofluorescence

    Visualization of the HCMV genome through in situ hybridization in NHDFCs and hiPSCs. (A–C) NHDFCs. (A) Control (uninfected). (B) HCMV (AD169) infection at an MOI of 1, 1 h post-infection. (C) HCMV (AD169) infection at an MOI of 10. The HCMV genome was visualized with GFP (green). (D–F) hiPSCs. (D) Control (uninfected). (E) HCMV (AD169) infection at an MOI of 1, 1 h post-infection. (F) HCMV (AD169) infection at an MOI of 10. The HCMV genome was visualized with GFP (green). Scale bar: 25 µm. GFP, green fluorescent protein; HCMV, human cytomegalovirus; hiPSC, human induced pluripotent stem cell; MOI, multiplicity of infection; NHDFC, neonatal human dermal fibroblast cell.

    Journal: Journal of Virology

    Article Title: Human induced pluripotent stem cells are resistant to human cytomegalovirus infection primarily at the attachment level due to the reduced expression of cell-surface heparan sulfate

    doi: 10.1128/jvi.01278-23

    Figure Lengend Snippet: Visualization of the HCMV genome through in situ hybridization in NHDFCs and hiPSCs. (A–C) NHDFCs. (A) Control (uninfected). (B) HCMV (AD169) infection at an MOI of 1, 1 h post-infection. (C) HCMV (AD169) infection at an MOI of 10. The HCMV genome was visualized with GFP (green). (D–F) hiPSCs. (D) Control (uninfected). (E) HCMV (AD169) infection at an MOI of 1, 1 h post-infection. (F) HCMV (AD169) infection at an MOI of 10. The HCMV genome was visualized with GFP (green). Scale bar: 25 µm. GFP, green fluorescent protein; HCMV, human cytomegalovirus; hiPSC, human induced pluripotent stem cell; MOI, multiplicity of infection; NHDFC, neonatal human dermal fibroblast cell.

    Article Snippet: The HCMV strain AD169, provided by the Department of Regenerative and Infectious pathology, Hamamatsu University School of Medicine, was cultivated in human embryonic lung fibroblasts using an MOI of 0.01.

    Techniques: In Situ Hybridization, Infection