Journal: Infectious Agents and Cancer

Article Title: Is human cytomegalovirus associated with breast cancer progression?

doi: 10.1186/1750-9378-8-12

Figure Lengend Snippet: Quality control for breast carcinomas and fibroadenomas. Multiplex PCR for GADPH. ( A ) Lines 1−9 and 19−26 breast carcinomas; 10−18 fibroadenomas. ( B ) Lines 27, 37−45 breast carcinomas; 19−30, 46 and 47 fibroadenomas. Base pairs (bp); control positive (+), HCMV strain AD-169 (ATCC® VR-583); control negative (−), DNA white blood cells from healthy individual (blood donor) and reaction mix without DNA.

Article Snippet: Positive control was done with HCMV strain AD-169 (ATCC VR-583).

Techniques: Multiplex Assay

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Inhibition activity of dendrimers against HCMV AD−169 . Plaque reduction assays were performed in MRC-5 cells for 6 days treatments. MRC-5 cells were pre-treated for 1 h at 37 ºC with increased concentrations of G1-S4, G2-S16 and G2-S24P PCDs (1, 5 and 10 µM) and infected with 60 PFU at 37 ºC. Plaques were counted after 6 days with a methylene blue dye. Percentage of infection is represented as the number of plaques in treatments in regard of the control of infection. CI, Control of Infection; NT, non-treated control. The mean values (mean ± SD) of at least four independent experiments are shown (* p < 0.05; ** p < 0.005; *** p < 0.001)

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Inhibition, Activity Assay, Infection

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Representative images of HCMV AD - 169 plaques in treatments with G2-S16 dendrimer. a Optical and b confocal representative images of MRC-5 cells. Briefly, MRC-5 cells were plated and treated with G2-S16 dendrimer (10 µM) for 1 h and infected with 60 PFU for 2 h at 37 ºC. After 6 days incubation a MRC-5 cells were dyed with methylene blue and images were taken on a DMIL Leica microscope at 4X augment. b MRC-5 cells were treated with HCMVpp65 ALEXA FLUOR® 488 Antibody Conjugated for viral particles visualization, phalloidin ALEXA FLUOR® 555 Conjugated and DAPI for membrane and nucleus localization. NT: non-treated; CI: control of infection

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Infection, Incubation, Microscopy

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Activity of PCDs at cell membrane-virus attachment. Plaque reduction assays were performed in MRC-5 cells for 6 days treatments. MRC-5 cells were pre-cooled at 4 ºC and pre-treated for 1 h at 4 ºC with increased concentrations of G1-S4, G2-S16 and G2-S24P PCDs (1, 5 and 10 µM) and infected with 60 PFU at 4 ºC. Plaques were counted after 6 days with a methylene blue dye. Percentage of infection is represented as the number of plaques in treatments in regard of the control of HCMV AD−169 infection. CI: Control of Infection NT = non-treated control. The mean values (mean ± SD) of at least four independent experiments are shown (* p < 0.05; ** p < 0.005; *** p < 0.001)

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Activity Assay, Infection

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Virucidal activity of G2-S16 or G2-S24P dendrimers against HCMV AD−169 . Plaque reduction assays were performed in MRC-5 cells for 6 days treatments. G2-S16 or G2-S24P dendrimer at 10 µM was incubated with 60 PFU for 2 h at 37 ºC. By several centrifugations at 12,000 RPM virus was pelleted, resuspended in fresh DMEM and added to MRC-5 cells for plaque reduction assays. Plaques were counted after 6 days with a methylene blue dye. Percentage of HCMV AD−169 infection is represented as the number of plaques in treatments in regard of the control of infection. Triton X-100 was used as a positive control of virus membrane disruption. CI: Control of Infection, NT: non-treated control. The mean values (mean ± SD) of at least four independent experiments are shown (* p < 0.05; ** p < 0.005; *** p < 0.001)

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Activity Assay, Incubation, Infection, Positive Control

Journal: Journal of Nanobiotechnology

Article Title: Nanotechnology against human cytomegalovirus in vitro: polyanionic carbosilane dendrimers as antiviral agents

doi: 10.1186/s12951-021-00809-4

Figure Lengend Snippet: Time of addition of G2-S16 or G2-S24P dendrimer and their combinations with ganciclovir. Plaque reduction assays were performed in MRC-5 cells for 6 days. MRC-5 cells were seeded, infected with 60 PFU and treated at different time points (0, 24, 48, 72 and 96 h post-infection) with either a ganciclovir, G2-S16 dendrimer and G2-S16 dendrimer + ganciclovir combination b ganciclovir, G2-S24P dendrimer, and G2-S24P + ganciclovir. Plaques were counted after 6 days with a methylene blue dye. Percentage of HCMV AD−169 infection is represented as the number of plaques in treatments in regard of the control of HCMV AD−169 infection. CI: Control of infection, NT: non-treated control. The mean values (mean ± SD) of at least four independent experiments are shown (* p < 0.05; ** p < 0.005; *** p < 0.001)

Article Snippet: The viral strain HCMV AD−169 (ATCC VR-538) was expanded and titrated in MRC-5 cell line by plaque assay with serial dilutions.

Techniques: Infection