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acute tnf toxicity  (Hycult Biotech)


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    Structured Review

    Hycult Biotech acute tnf toxicity
    L929 cells were incubated with recombinant mouse <t>TNF</t> in the presence or absence of 10 <t>µg/ml</t> <t>anti-TNFR1</t> ( A ) or with anti-TNFR1 in the presence of 0.1 ng/ml mouse TNF ( B ) and cell survival was determined by crystal violet staining. C, D : Female C57BL/6 wild type mice were pretreated with PBS or anti-TNFR1 (10 mg/kg, i.p.). After 2 h, PBS or murine TNF were injected i.v. (1 mg/kg) and body temperature ( C ) as well as survival of animals ( D ) were determined.
    Acute Tnf Toxicity, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acute tnf toxicity/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    acute tnf toxicity - by Bioz Stars, 2025-07
    92/100 stars

    Images

    1) Product Images from "Antibody-Mediated Inhibition of TNFR1 Attenuates Disease in a Mouse Model of Multiple Sclerosis"

    Article Title: Antibody-Mediated Inhibition of TNFR1 Attenuates Disease in a Mouse Model of Multiple Sclerosis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0090117

    L929 cells were incubated with recombinant mouse TNF in the presence or absence of 10 µg/ml anti-TNFR1 ( A ) or with anti-TNFR1 in the presence of 0.1 ng/ml mouse TNF ( B ) and cell survival was determined by crystal violet staining. C, D : Female C57BL/6 wild type mice were pretreated with PBS or anti-TNFR1 (10 mg/kg, i.p.). After 2 h, PBS or murine TNF were injected i.v. (1 mg/kg) and body temperature ( C ) as well as survival of animals ( D ) were determined.
    Figure Legend Snippet: L929 cells were incubated with recombinant mouse TNF in the presence or absence of 10 µg/ml anti-TNFR1 ( A ) or with anti-TNFR1 in the presence of 0.1 ng/ml mouse TNF ( B ) and cell survival was determined by crystal violet staining. C, D : Female C57BL/6 wild type mice were pretreated with PBS or anti-TNFR1 (10 mg/kg, i.p.). After 2 h, PBS or murine TNF were injected i.v. (1 mg/kg) and body temperature ( C ) as well as survival of animals ( D ) were determined.

    Techniques Used: Incubation, Recombinant, Staining, Injection



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    92
    Hycult Biotech acute tnf toxicity
    L929 cells were incubated with recombinant mouse <t>TNF</t> in the presence or absence of 10 <t>µg/ml</t> <t>anti-TNFR1</t> ( A ) or with anti-TNFR1 in the presence of 0.1 ng/ml mouse TNF ( B ) and cell survival was determined by crystal violet staining. C, D : Female C57BL/6 wild type mice were pretreated with PBS or anti-TNFR1 (10 mg/kg, i.p.). After 2 h, PBS or murine TNF were injected i.v. (1 mg/kg) and body temperature ( C ) as well as survival of animals ( D ) were determined.
    Acute Tnf Toxicity, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acute tnf toxicity/product/Hycult Biotech
    Average 92 stars, based on 1 article reviews
    acute tnf toxicity - by Bioz Stars, 2025-07
    92/100 stars
      Buy from Supplier

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    L929 cells were incubated with recombinant mouse TNF in the presence or absence of 10 µg/ml anti-TNFR1 ( A ) or with anti-TNFR1 in the presence of 0.1 ng/ml mouse TNF ( B ) and cell survival was determined by crystal violet staining. C, D : Female C57BL/6 wild type mice were pretreated with PBS or anti-TNFR1 (10 mg/kg, i.p.). After 2 h, PBS or murine TNF were injected i.v. (1 mg/kg) and body temperature ( C ) as well as survival of animals ( D ) were determined.

    Journal: PLoS ONE

    Article Title: Antibody-Mediated Inhibition of TNFR1 Attenuates Disease in a Mouse Model of Multiple Sclerosis

    doi: 10.1371/journal.pone.0090117

    Figure Lengend Snippet: L929 cells were incubated with recombinant mouse TNF in the presence or absence of 10 µg/ml anti-TNFR1 ( A ) or with anti-TNFR1 in the presence of 0.1 ng/ml mouse TNF ( B ) and cell survival was determined by crystal violet staining. C, D : Female C57BL/6 wild type mice were pretreated with PBS or anti-TNFR1 (10 mg/kg, i.p.). After 2 h, PBS or murine TNF were injected i.v. (1 mg/kg) and body temperature ( C ) as well as survival of animals ( D ) were determined.

    Article Snippet: To evaluate the capability of anti-TNFR1 treatment to block acute TNF toxicity we used the antibody HM1097 (Hycult Biotech, Uden the Netherlands), which has been described previously as a specific antagonist for mouse TNFR1 (55R-170), .

    Techniques: Incubation, Recombinant, Staining, Injection