acui  (New England Biolabs)


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    New England Biolabs acui
    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    acui  (New England Biolabs)


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    New England Biolabs acui
    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs acui
    Development and validation of <t>One-pot</t> <t>DTECT</t> (A) Schematic representation of the four independent steps required to capture signatures with DTECTv1. (B) Measure of the capture efficiency of the SARS-CoV-2 E484K signature using a variant-specific adaptor or a nonspecific adaptor as a control using DTECTv1, or with the omission of <t>AcuI,</t> bead isolation, or ligase, as indicated. Error bars represent SD of two independent experiments. Individual data points are shown. (C) Measure of the capture specificity of the SARS-CoV-2 E484 or E484K signatures. DTECTv1 was used as a control (yellow). Experiments were conducted with or without AcuI, bead isolation, or DNA ligase, as indicated. Error bars represent SD of two independent experiments. Individual data points are shown. (D) Time-course experiment of AcuI digestion in DTECTv1. Error bars represent SD of two independent experiments. (E) Time-course experiment of heat inactivation of AcuI in DTECTv1. Error bars represent SD of two independent experiments. (F) Time-course experiment of adaptor ligation in DTECTv1. Error bars represent SD of two independent experiments. (G) Schematic representation of the experiment presented in (H). (H) Comparison of the capture specificity between DTECTv2 and One-pot DTECT using the indicated adaptor concentration. Error bars represent SD of three independent experiments. Individual data points are shown. (I) Modification of buffer composition in One-pot DTECT. Error bars represent SD of two independent experiments. (J) Capture efficiency of One-pot DTECT containing a DNA competitor fragment (dark green and dark red) or a control DNA fragment (light green and light red). Error bars represent SD of three independent experiments. (K) Schematic representation of the different modifications applied to DTECTv2 to allow the development of One-pot DTECT. (L) Schematic representation of the different steps in DTECTv1, DTECTv2, and One-pot DTECT. The approximate time required for each step is also indicated.
    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    acui - by Bioz Stars, 2024-06
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    1) Product Images from "One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics"

    Article Title: One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2024.100698

    Development and validation of One-pot DTECT (A) Schematic representation of the four independent steps required to capture signatures with DTECTv1. (B) Measure of the capture efficiency of the SARS-CoV-2 E484K signature using a variant-specific adaptor or a nonspecific adaptor as a control using DTECTv1, or with the omission of AcuI, bead isolation, or ligase, as indicated. Error bars represent SD of two independent experiments. Individual data points are shown. (C) Measure of the capture specificity of the SARS-CoV-2 E484 or E484K signatures. DTECTv1 was used as a control (yellow). Experiments were conducted with or without AcuI, bead isolation, or DNA ligase, as indicated. Error bars represent SD of two independent experiments. Individual data points are shown. (D) Time-course experiment of AcuI digestion in DTECTv1. Error bars represent SD of two independent experiments. (E) Time-course experiment of heat inactivation of AcuI in DTECTv1. Error bars represent SD of two independent experiments. (F) Time-course experiment of adaptor ligation in DTECTv1. Error bars represent SD of two independent experiments. (G) Schematic representation of the experiment presented in (H). (H) Comparison of the capture specificity between DTECTv2 and One-pot DTECT using the indicated adaptor concentration. Error bars represent SD of three independent experiments. Individual data points are shown. (I) Modification of buffer composition in One-pot DTECT. Error bars represent SD of two independent experiments. (J) Capture efficiency of One-pot DTECT containing a DNA competitor fragment (dark green and dark red) or a control DNA fragment (light green and light red). Error bars represent SD of three independent experiments. (K) Schematic representation of the different modifications applied to DTECTv2 to allow the development of One-pot DTECT. (L) Schematic representation of the different steps in DTECTv1, DTECTv2, and One-pot DTECT. The approximate time required for each step is also indicated.
    Figure Legend Snippet: Development and validation of One-pot DTECT (A) Schematic representation of the four independent steps required to capture signatures with DTECTv1. (B) Measure of the capture efficiency of the SARS-CoV-2 E484K signature using a variant-specific adaptor or a nonspecific adaptor as a control using DTECTv1, or with the omission of AcuI, bead isolation, or ligase, as indicated. Error bars represent SD of two independent experiments. Individual data points are shown. (C) Measure of the capture specificity of the SARS-CoV-2 E484 or E484K signatures. DTECTv1 was used as a control (yellow). Experiments were conducted with or without AcuI, bead isolation, or DNA ligase, as indicated. Error bars represent SD of two independent experiments. Individual data points are shown. (D) Time-course experiment of AcuI digestion in DTECTv1. Error bars represent SD of two independent experiments. (E) Time-course experiment of heat inactivation of AcuI in DTECTv1. Error bars represent SD of two independent experiments. (F) Time-course experiment of adaptor ligation in DTECTv1. Error bars represent SD of two independent experiments. (G) Schematic representation of the experiment presented in (H). (H) Comparison of the capture specificity between DTECTv2 and One-pot DTECT using the indicated adaptor concentration. Error bars represent SD of three independent experiments. Individual data points are shown. (I) Modification of buffer composition in One-pot DTECT. Error bars represent SD of two independent experiments. (J) Capture efficiency of One-pot DTECT containing a DNA competitor fragment (dark green and dark red) or a control DNA fragment (light green and light red). Error bars represent SD of three independent experiments. (K) Schematic representation of the different modifications applied to DTECTv2 to allow the development of One-pot DTECT. (L) Schematic representation of the different steps in DTECTv1, DTECTv2, and One-pot DTECT. The approximate time required for each step is also indicated.

    Techniques Used: Variant Assay, Isolation, Ligation, Comparison, Concentration Assay, Modification

    Development and validation of One-pot DTECT-LAMP (A) Schematic representation of One-pot DTECT-LAMP. One-pot DTECT-LAMP follows the same amplification and capture procedure as One-pot DTECT with only small modification variations in the AcuI-tagging primer and adaptor sequences. AcuI-tagging primers contain F2 and F3 LAMP sequences at their 5′ end, and adaptors contain F1, B1, B2, and B3 LAMP sequences. (B) One-pot DTECT-LAMP reactions were conducted to capture the SARS-CoV-2 E484 or E484K signatures. (C) Capture of the SARS-CoV-2 E484K variant using One-pot DTECT-LAMP with real-time quantification of changes in absorbance.
    Figure Legend Snippet: Development and validation of One-pot DTECT-LAMP (A) Schematic representation of One-pot DTECT-LAMP. One-pot DTECT-LAMP follows the same amplification and capture procedure as One-pot DTECT with only small modification variations in the AcuI-tagging primer and adaptor sequences. AcuI-tagging primers contain F2 and F3 LAMP sequences at their 5′ end, and adaptors contain F1, B1, B2, and B3 LAMP sequences. (B) One-pot DTECT-LAMP reactions were conducted to capture the SARS-CoV-2 E484 or E484K signatures. (C) Capture of the SARS-CoV-2 E484K variant using One-pot DTECT-LAMP with real-time quantification of changes in absorbance.

    Techniques Used: Amplification, Modification, Variant Assay


    Figure Legend Snippet:

    Techniques Used: Recombinant, SYBR Green Assay, DNA Extraction, Staining, Plasmid Preparation, Magnetic Beads, Next-Generation Sequencing, Clone Assay, Real-time Polymerase Chain Reaction, Imaging

    acui  (New England Biolabs)


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    New England Biolabs acui

    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics"

    Article Title: One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2024.100698


    Figure Legend Snippet:

    Techniques Used: Recombinant, SYBR Green Assay, DNA Extraction, Staining, Plasmid Preparation, Magnetic Beads, Next-Generation Sequencing, Clone Assay, Real-time Polymerase Chain Reaction, Imaging

    acui  (New England Biolabs)


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    New England Biolabs acui

    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics"

    Article Title: One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2024.100698


    Figure Legend Snippet:

    Techniques Used: Recombinant, SYBR Green Assay, DNA Extraction, Staining, Plasmid Preparation, Magnetic Beads, Next-Generation Sequencing, Clone Assay, Real-time Polymerase Chain Reaction, Imaging

    acui  (New England Biolabs)


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    New England Biolabs acui
    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    acui  (New England Biolabs)


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    New England Biolabs acui

    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acui/product/New England Biolabs
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    1) Product Images from "One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics"

    Article Title: One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2024.100698


    Figure Legend Snippet:

    Techniques Used: Recombinant, SYBR Green Assay, DNA Extraction, Staining, Plasmid Preparation, Magnetic Beads, Next-Generation Sequencing, Clone Assay, Real-time Polymerase Chain Reaction, Imaging

    acui  (New England Biolabs)


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    New England Biolabs acui

    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics"

    Article Title: One-pot DTECT enables rapid and efficient capture of genetic signatures for precision genome editing and clinical diagnostics

    Journal: Cell Reports Methods

    doi: 10.1016/j.crmeth.2024.100698


    Figure Legend Snippet:

    Techniques Used: Recombinant, SYBR Green Assay, DNA Extraction, Staining, Plasmid Preparation, Magnetic Beads, Next-Generation Sequencing, Clone Assay, Real-time Polymerase Chain Reaction, Imaging

    acui  (New England Biolabs)


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    Acui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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