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TaKaRa activation domain fusion vectors pgbkt7
Mutation of the NLS within DUSP5 does not affect the ability of DUSP5 to either interact with or dephosphorylate ERK2. (A) Yeast two-hybrid assays. Either wild-type (WT) <t>pGBKT7.DUSP5</t> or the indicated mutants were transformed into PJ69-4A and mated with
Activation Domain Fusion Vectors Pgbkt7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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1) Product Images from "Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5"

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5

Journal:

doi: 10.1128/MCB.25.5.1830-1845.2005

Mutation of the NLS within DUSP5 does not affect the ability of DUSP5 to either interact with or dephosphorylate ERK2. (A) Yeast two-hybrid assays. Either wild-type (WT) pGBKT7.DUSP5 or the indicated mutants were transformed into PJ69-4A and mated with
Figure Legend Snippet: Mutation of the NLS within DUSP5 does not affect the ability of DUSP5 to either interact with or dephosphorylate ERK2. (A) Yeast two-hybrid assays. Either wild-type (WT) pGBKT7.DUSP5 or the indicated mutants were transformed into PJ69-4A and mated with

Techniques Used: Mutagenesis, Transformation Assay

DUSP5 interacts specifically and directly with the ERK1 and ERK2 MAP kinases. (A) Yeast two-hybrid assays. pGBKT7.DUSP5 was transformed into PJ69-4A and mated with PJ69-4α expressing the GAL4 activation domain (AD) fusions pGADT7.ERK1, pGADT7.ERK2,
Figure Legend Snippet: DUSP5 interacts specifically and directly with the ERK1 and ERK2 MAP kinases. (A) Yeast two-hybrid assays. pGBKT7.DUSP5 was transformed into PJ69-4A and mated with PJ69-4α expressing the GAL4 activation domain (AD) fusions pGADT7.ERK1, pGADT7.ERK2,

Techniques Used: Transformation Assay, Expressing, Activation Assay

Related Articles

Clone Assay:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. GFP expression constructs were generated by cloning the DUSP5 cDNA as an XhoI-KpnI fragment into vector pECFP-N1 (Clontech).

Amplification:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: The human DUSP5 cDNA was amplified by PCR with Image clone 5808876 ( ) as a template. .. The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech).

Polymerase Chain Reaction:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: .. The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. The DUSP5 cDNA was ligated into mammalian expression vector pSG5 (Stratagene) in frame with the Myc tag sequence by using EcoRI and XhoI restriction sites.

Construct:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: Paragraph title: DNA constructs. ... The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech).

Produced:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. The various point mutations of DUSP5 were produced by overlap extension PCR ( ) with the following complementary primers (only the forward primer is shown) (mutations are in bold type): C263S, 5′-AAGGTCCTGGTCCAC AGT GAGGCTGGGATCTCCCGT-3′; D232N, 5′-CACTACAAATGGATCCCTGTGGAA AAC AGCCACACG-3′; R53,54A, 5′-GTCAACCTCAACTCGGTGGTGCTG GCGGCG GCCCGGGGCGGCGCGGTGTCG-3′; R53A, 5′-GTCAACCTCAACTCGGTGGTGCTG GCG CGGGCCCGGGGCGGCGCGGTGTCG-3′; and R54A, 5′-GTCAACCTCAACTCGGTGGTGCTGCGG GCG GCCCGGGGCGGCGCGGTGTCG-3′; appropriate external flanking primers also were used.

Activation Assay:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: .. The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. The DUSP5 cDNA was ligated into mammalian expression vector pSG5 (Stratagene) in frame with the Myc tag sequence by using EcoRI and XhoI restriction sites.

Generated:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. GFP expression constructs were generated by cloning the DUSP5 cDNA as an XhoI-KpnI fragment into vector pECFP-N1 (Clontech).

Expressing:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: .. The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. The DUSP5 cDNA was ligated into mammalian expression vector pSG5 (Stratagene) in frame with the Myc tag sequence by using EcoRI and XhoI restriction sites.

Sequencing:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. The DUSP5 cDNA was ligated into mammalian expression vector pSG5 (Stratagene) in frame with the Myc tag sequence by using EcoRI and XhoI restriction sites.

Binding Assay:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: .. The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. The DUSP5 cDNA was ligated into mammalian expression vector pSG5 (Stratagene) in frame with the Myc tag sequence by using EcoRI and XhoI restriction sites.

Plasmid Preparation:

Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5
Article Snippet: .. The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech). .. The DUSP5 cDNA was ligated into mammalian expression vector pSG5 (Stratagene) in frame with the Myc tag sequence by using EcoRI and XhoI restriction sites.

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    TaKaRa activation domain fusion vectors pgbkt7
    Mutation of the NLS within DUSP5 does not affect the ability of DUSP5 to either interact with or dephosphorylate ERK2. (A) Yeast two-hybrid assays. Either wild-type (WT) <t>pGBKT7.DUSP5</t> or the indicated mutants were transformed into PJ69-4A and mated with
    Activation Domain Fusion Vectors Pgbkt7, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/activation domain fusion vectors pgbkt7/product/TaKaRa
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    activation domain fusion vectors pgbkt7 - by Bioz Stars, 2020-02
    79/100 stars
      Buy from Supplier

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    Mutation of the NLS within DUSP5 does not affect the ability of DUSP5 to either interact with or dephosphorylate ERK2. (A) Yeast two-hybrid assays. Either wild-type (WT) pGBKT7.DUSP5 or the indicated mutants were transformed into PJ69-4A and mated with

    Journal:

    Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5

    doi: 10.1128/MCB.25.5.1830-1845.2005

    Figure Lengend Snippet: Mutation of the NLS within DUSP5 does not affect the ability of DUSP5 to either interact with or dephosphorylate ERK2. (A) Yeast two-hybrid assays. Either wild-type (WT) pGBKT7.DUSP5 or the indicated mutants were transformed into PJ69-4A and mated with

    Article Snippet: The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech).

    Techniques: Mutagenesis, Transformation Assay

    DUSP5 interacts specifically and directly with the ERK1 and ERK2 MAP kinases. (A) Yeast two-hybrid assays. pGBKT7.DUSP5 was transformed into PJ69-4A and mated with PJ69-4α expressing the GAL4 activation domain (AD) fusions pGADT7.ERK1, pGADT7.ERK2,

    Journal:

    Article Title: Specific Inactivation and Nuclear Anchoring of Extracellular Signal-Regulated Kinase 2 by the Inducible Dual-Specificity Protein Phosphatase DUSP5

    doi: 10.1128/MCB.25.5.1830-1845.2005

    Figure Lengend Snippet: DUSP5 interacts specifically and directly with the ERK1 and ERK2 MAP kinases. (A) Yeast two-hybrid assays. pGBKT7.DUSP5 was transformed into PJ69-4A and mated with PJ69-4α expressing the GAL4 activation domain (AD) fusions pGADT7.ERK1, pGADT7.ERK2,

    Article Snippet: The PCR product was subcloned as an NdeI-XhoI fragment into bacterial expression vector pET15b (Novagen) and Matchmaker System 3 DNA binding domain and activation domain fusion vectors pGBKT7 and pGADT7 (Clontech).

    Techniques: Transformation Assay, Expressing, Activation Assay